ViewGene
Locus Name:
GeneID:
Location:

GeneID:998
TrivialName: PA14_63160
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63160
PAO1 Ortholog Locus:PA4777
Sequence Length:1434
Protein Length:477
Start: 5636684
Stop: 5638117
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08163.1|AE004891_5 (AE004891) probable two-component sensor [Pseudomonas aeruginosa PAO1]
Identities = 472/477 (98%), Positives = 474/477 (99%)
Sequence:
ATGCCGCGTACCGCCGTCCCCTCCGTCCGCCGGCGCCTGCTGATCAACCTGCTGGTCGGCTTCGTGCTGTGC
TGGCTGAGCGTGGCGGCGCTGACCTACCACCTCTCGCTGAAGCAGGTGAACCGCCTGTTCGACGACGACATG
GTGGACTTCGGCGAAGCCGCCCTGCGCCTGCTCGACCTTGCCACCGAAGACCAGGCCAGCGAGGACGGCTCG
ATCACCGAGATCATCGAACGCAGCCGCGAGGCGATCCaGGGCCTGCCCCTGCTGCGCCGCGAAAGCGCCCTC
GGCTACGCCCTGTGGCGCGACGGCCAGCCGCTGCTGTCGAGCCTCAACCTGCCGCCGGAGATCACGGCCCAG
GGCCCCGGCTTCAGCACCGTGGAAGCCCAGGGCACCCACTGGCGGGTGCTCCAGCTGAACATCGACGGCTTC
CAGATCTGGATCAGCGAAAACCTGATCTACCGCCAGCACACCATGAACCTGCTGCTGTTCTATTCGCTGTTC
CCGCTGCTGCTGGCGCTGCCGTTGCTCGGCGGCCTGGTCTGGTTCGGCGTTGCCCGCGGCCTGGCGCCGCTA
CGCGAAGTGCAGGCCGAGGTCCAGCAGCGCTCCGCGCGACACCTGCAGCCGATCGCGGTGGAGGCGGTGCCG
CTGGAGATCCGCGGCCTCATCGACGAACTCAACCTCCTGCTGGAGCGCCTGCGCACCGCCCTCGAGGCCGAA
CGCCGACTGACCAGCGACGCCGCCCATGAGATCCGCACGCCGCTGGCCAGCCTGCGCACCCATGCCCAGGTC
GCGCTGCGTTCGGAAGACCCCAAGGCCCACGCCCGCGGCCTGCTGCAAGTCAGTCGCAGCGTCGAGCGGATC
AGCACCTTGATGGAGCAGATCCTGCTTCTCGCCCGCCTCGACGGCGACGCCCTGCTGGAGCAATTCCACCCG
GTCAACCTCGCCACCCTGGCCGAAGACGTACTCTCCGAACTGGCGCGCCAGGCCATCGACAAGGACATCGAG
CTGTCGTTGCACCAGGAGACCGTGCACGTGATGGGCATCGACCTGTGGCTGAAGGCGATGGTCGGCAACCTG
GTGGGCAACGCCCTGCGCTACACACCGGCCGGGGGCCAGGTCGAGATCCGCGTCGAGAATCGCGCCCAGCAC
GCCGTGCTGCGGGTGCGCGACAACGGCCCCGGGGTCGCCCTGGAAGAGCAGCAGGCGATCTTCACCCGCTTC
TACCGCAGCCCCGCCACCAGCAGCGGCGAGGGCAGCGGCCTGGGCCTGCCGATCGTCAAGCGCATCGTCGAA
CTGCACTTCGGCAGTATCGGCCTGGGCAAGGGACTGGAGGGCAAAGGGCTGGAAGTACAGGTGTTCCTGCCG
AAGACCCAGCCGGACGCGACGCGGCCGCCGGCCAGAGGTCCGGACAGCGGGCGGTCACATATCTGA
Translation:
MPRTAVPSVRRRLLINLLVGFVLCWLSVAALTYHLSLKQVNRLFDDDMVDFGEAALRLLDLATEDQASEDGS
ITEIIERSREAIQGLPLLRRESALGYALWRDGQPLLSSLNLPPEITAQGPGFSTVEAQGTHWRVLQLNIDGF
QIWISENLIYRQHTMNLLLFYSLFPLLLALPLLGGLVWFGVARGLAPLREVQAEVQQRSARHLQPIAVEAVP
LEIRGLIDELNLLLERLRTALEAERRLTSDAAHEIRTPLASLRTHAQVALRSEDPKAHARGLLQVSRSVERI
STLMEQILLLARLDGDALLEQFHPVNLATLAEDVLSELARQAIDKDIELSLHQETVHVMGIDLWLKAMVGNL
VGNALRYTPAGGQVEIRVENRAQHAVLRVRDNGPGVALEEQQAIFTRFYRSPATSSGEGSGLGLPIVKRIVE
LHFGSIGLGKGLEGKGLEVQVFLPKTQPDATRPPARGPDSGRSHI*
AnnotationID:1007GeneID:998
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:56
Gene Name:pmrB
Confidence Code:1
GeneProduct:two-component sensor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0642,smart00387,pfam02518
Secondary Functional Category(ies):antibiotic resistance and susceptibility
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
COG0642, BaeS, Signal transduction histidine kinase 
[Signal transduction mechanisms]
CD-Length = 336 residues, 100.0% aligned
Score =  150 bits (378), Expect = 4e-37
Identities = 111/342 (32%), Positives = 164/342 (47%), Gaps = 9/342 (2%)

smart00387, HATPase_c, Histidine kinase-like ATPases
CD-Length = 112 residues, 100.0% aligned
Score =  102 bits (255), Expect = 1e-22
Identities = 43/113 (38%), Positives = 65/113 (57%), Gaps = 2/113 (1%)

pfam02518, HATPase_c, Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase. 
CD-Length = 111 residues, 100.0% aligned
Score = 95.1 bits (236), Expect = 2e-20
Identities = 39/112 (34%), Positives = 64/112 (57%), Gaps = 2/112 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1999




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19141




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37685




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:998_cdd.html


    		




 
URL:

















    ReferenceID:37704




  
Author/Investigator(s):
        Moskowitz SM, Ernst RK, Miller SI.





  
Title:
        PmrAB, a two-component regulatory system of Pseudomonas aeruginosa that modulates resistance to cationic antimicrobial peptides and addition of aminoarabinose to lipid A.




  
PubMed:
        14702327


MedLine:
        




    
Source:
J Bacteriol. 2004 Jan;186(2):575-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=14702327

















    ReferenceID:37707




  
Author/Investigator(s):
        McPhee JB, Lewenza S, Hancock RE.





  
Title:
        Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa.




  
PubMed:
        14507375


MedLine:
        




    
Source:
Mol Microbiol. 2003 Oct;50(1):205-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14507375









           
Homologs By Global Alignment 

           

       



     
Gene ID:998
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1797
                           PA4777_tr
                           translation of PA4777
                           477
                           98.95
                           99.37
                           0
                           2372.0
     

     
     
62753
                           gb|AAG08163.1|AE004891_5
                           (AE004891) probable two-component sensor [Pseudomonas aeruginosa PAO1]
                           477
                           98.95
                           99.37
                           0
                           2372.0
     

     
     
62754
                           dbj|BAB53616.1|
                           (AP003013) two-component system, sensor protein [Mesorhizobium loti]
                           519
                           30.44
                           42.77
                           109
                           496.0
     

     






      

          
GC ORFID: 44000How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4777Glimmer Score: 

          
Start: 4900364Stop: 4901797

          
Length: 1434


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4777 translation of PA4777Homolog Bit Score 918.0

          
Other Homologs:  t_PA4777 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGCGTACCGCCGTCCCCTCCGTCCGCCGGCGCCTGCTGATCAACCTGCTGGTCGGCTTCGTGCTGTGC 
TGGCTGAGCGTGGCGGCGCTGACCTACCACCTCTCGCTGAAGCAGGTGAACCGCCTGTTCGACGACGACATG 
GTGGACTTCGGCGAAGCCGCCCTGCGCCTGCTCGACCTTGCCACCGAAGACCAGGCCAGCGAGGACGGCTCG 
ATCACCGAGATCATCGAACGCAGCCGCGAGGCGATCCaGGGCCTGCCCCTGCTGCGCCGCGAAAGCGCCCTC 
GGCTACGCCCTGTGGCGCGACGGCCAGCCGCTGCTGTCGAGCCTCAACCTGCCGCCGGAGATCACGGCCCAG 
GGCCCCGGCTTCAGCACCGTGGAAGCCCAGGGCACCCACTGGCGGGTGCTCCAGCTGAACATCGACGGCTTC 
CAGATCTGGATCAGCGAAAACCTGATCTACCGCCAGCACACCATGAACCTGCTGCTGTTCTATTCGCTGTTC 
CCGCTGCTGCTGGCGCTGCCGTTGCTCGGCGGCCTGGTCTGGTTCGGCGTTGCCCGCGGCCTGGCGCCGCTA 
CGCGAAGTGCAGGCCGAGGTCCAGCAGCGCTCCGCGCGACACCTGCAGCCGATCGCGGTGGAGGCGGTGCCG 
CTGGAGATCCGCGGCCTCATCGACGAACTCAACCTCCTGCTGGAGCGCCTGCGCACCGCCCTCGAGGCCGAA 
CGCCGACTGACCAGCGACGCCGCCCATGAGATCCGCACGCCGCTGGCCAGCCTGCGCACCCATGCCCAGGTC 
GCGCTGCGTTCGGAAGACCCCAAGGCCCACGCCCGCGGCCTGCTGCAAGTCAGTCGCAGCGTCGAGCGGATC 
AGCACCTTGATGGAGCAGATCCTGCTTCTCGCCCGCCTCGACGGCGACGCCCTGCTGGAGCAATTCCACCCG 
GTCAACCTCGCCACCCTGGCCGAAGACGTACTCTCCGAACTGGCGCGCCAGGCCATCGACAAGGACATCGAG 
CTGTCGTTGCACCAGGAGACCGTGCACGTGATGGGCATCGACCTGTGGCTGAAGGCGATGGTCGGCAACCTG 
GTGGGCAACGCCCTGCGCTACACACCGGCCGGGGGCCAGGTCGAGATCCGCGTCGAGAATCGCGCCCAGCAC 
GCCGTGCTGCGGGTGCGCGACAACGGCCCCGGGGTCGCCCTGGAAGAGCAGCAGGCGATCTTCACCCGCTTC 
TACCGCAGCCCCGCCACCAGCAGCGGCGAGGGCAGCGGCCTGGGCCTGCCGATCGTCAAGCGCATCGTCGAA 
CTGCACTTCGGCAGTATCGGCCTGGGCAAGGGACTGGAGGGCAAAGGGCTGGAAGTACAGGTGTTCCTGCCG 
AAGACCCAGCCGGACGCGACGCGGCCGCCGGCCAGAGGTCCGGACAGCGGGCGGTCACATATCTGA