ViewGene
Locus Name:
GeneID:
Location:

GeneID:995
TrivialName: PA14_38140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38140
PAO1 Ortholog Locus:PA2040
Sequence Length:1377
Protein Length:458
Start: 3403511
Stop: 3402135
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05428.1|AE004630_5 (AE004630) probable glutamine synthetase [Pseudomonas aeruginosa PAO1]
Identities = 455/458 (99%), Positives = 457/458 (99%)
Sequence:
ATGTCGGTACCCCTGCGTGCCGTTCAGCTCACTGAGCCGAGCCTGTTCCTTCAGGAACATCCCGAGGTCCAG
TTCGTCGACCTTCTGATTTCAGACATGAATGGCGTGGTTCGCGGCAAGCGAATCGAACGCAACAGTCTGCCC
AAAGTCTTCGAAAAAGGTATCAACCTTCCCGCTTCCCTGTTCGCCCTCGATATCACCGGCTCCACCGTAGAG
AGTACCGGCCTGGGTCTCGACATCGGCGACGCCGACCGGATCTGCTACCCGATCCCCGGCACCCTCTCCATG
GAACCCTGGCAGAAGCGGCCAACCGCGCAACTGCTGATGACCATGCATGAGCTGGAAGGTGATCCCTTCTTC
GCCGACCCCCGCGAAGTCCTGCGCCAGGTGGTGGCCAGGTTCACCGAGATGGAACTGACCATCGTCGCCGCC
TTCGAGCTGGAGTTCTACCTGATCGACCAGGAGAACGTGAACGGCCGGCCGCAGCCGCCGCGCTCGCCGATC
TCCGGCAAACGTCCGCAGTCGGTGCAGGTGTATTCCATCGACGACCTCGACGAATACGTCGAGTGCCTCCAG
GACATCATCGACGGCGCCCGCGCCCAGGGCATCCCGGCCGACGCCATCGTCGCCGAATCCGCACCGGCGCAG
TTCGAGGTCAACCTGCATCACGTCGCCGACCCGATGAAGGCCTGCGACTATGCGGTGCTGCTCAAGCGCCTG
ATCAAGAACATCGCCTACGACCACGAGATGGACACCACCTTCATGGCCAAGCCCTATCCGGGCCAGGCTGGG
AACGGACTGCACGTGCACATCTCGCTGCTCGACAAGCACGGCAACAACATCTTCACCGGCGAGGATCCCGAG
CAGAACGCCGCATTGCGCCATGCGATCGGCGGCGTGCTCGAGACCCTGCCGGCCTCCATGGCCTTCCTCTGC
CCGAACGTCAACTCCTACCGCCGCTTCGGTTCGCAGTTCTACGTGCCGAACGCGCCGAGCTGGGGCCTGGAC
AACCGCACCGTGGCCCTGCGCGTGCCCACCGGCAGCCCGGACGCGGTACGCCTGGAACATCGCGTCGCCGGC
GCCGACGCCAACCCCTACCTGCTGCTGGCCTCGGTGCTGGCCGGGGTACACCACGGCCTGACCAACCAGGTC
GAGCCGGGCGCGCCGATCGAAGGCAACTCCTACGAGCAGTTGGAGCCGAGCCTGCCGAACAACCTGCGCGAC
GCCCTGCGTGAACTGGACGACAGCGAGATCCTGGCGAAGTACATCGATCCGAAGTACATCGACATCTTCGTC
GCCTGCAAGGAAAGCGAGCTGGAGGAGTTCGAGTACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCAC
ACCGTGTGA
Translation:
MSVPLRAVQLTEPSLFLQEHPEVQFVDLLISDMNGVVRGKRIERNSLPKVFEKGINLPASLFALDITGSTVE
STGLGLDIGDADRICYPIPGTLSMEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVARFTEMELTIVAA
FELEFYLIDQENVNGRPQPPRSPISGKRPQSVQVYSIDDLDEYVECLQDIIDGARAQGIPADAIVAESAPAQ
FEVNLHHVADPMKACDYAVLLKRLIKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISLLDKHGNNIFTGEDPE
QNAALRHAIGGVLETLPASMAFLCPNVNSYRRFGSQFYVPNAPSWGLDNRTVALRVPTGSPDAVRLEHRVAG
ADANPYLLLASVLAGVHHGLTNQVEPGAPIEGNSYEQLEPSLPNNLRDALRELDDSEILAKYIDPKYIDIFV
ACKESELEEFEYSISDLEYNWYLHTV*
AnnotationID:1004GeneID:995
AnnotatorUID: danlee
Modification Date/Time: 2005-09-27 17:05:01
Gene Name:
Confidence Code:3
GeneProduct:putative glutamine synthetase
Cell Localization:()
Synonyms:PA2040
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0174,pfam00120
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70749.1|AE016718_8 (AE016793) glutamine synthetase, putative [Pseudomonas putida
           KT2440]
          Length = 458

 Score =  839 bits (2168), Expect = 0.0
 Identities = 410/458 (89%), Positives = 433/458 (94%)

>gb|AAN70864.1|AE016730_10 (AE016794) glutamine synthetase, putative [Pseudomonas putida
           KT2440]
          Length = 460

 Score =  776 bits (2003), Expect = 0.0
 Identities = 380/458 (82%), Positives = 415/458 (90%), Gaps = 1/458 (0%)

>gb|AAC74379.1| (AE000228) putative glutamine synthetase (EC 6.3.1.2) [Escherichia
           coli K12]
          Length = 498

 Score =  385 bits (988), Expect = e-106
 Identities = 196/445 (44%), Positives = 287/445 (64%), Gaps = 2/445 (0%)






 
Structural Features:
COG0174, GlnA, Glutamine synthetase [Amino acid transport and metabolism]
CD-Length = 443 residues,  99.3% aligned
Score =  410 bits (1056), Expect = 1e-115
Identities = 172/460 (37%), Positives = 254/460 (55%), Gaps = 34/460 (7%)

pfam00120, Gln-synt_C, Glutamine synthetase, catalytic domain. 
CD-Length = 260 residues,  99.6% aligned
Score =  253 bits (649), Expect = 2e-68
Identities = 96/263 (36%), Positives = 135/263 (51%), Gaps = 7/263 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1993




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19135




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32666




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:995_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:995
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1790
                           PA2040_tr
                           translation of PA2040
                           458
                           99.34
                           99.78
                           0
                           2375.0
     

     
     
62672
                           gb|AAG05428.1|AE004630_5
                           (AE004630) probable glutamine synthetase [Pseudomonas aeruginosa PAO1]
                           458
                           99.34
                           99.78
                           0
                           2375.0
     

     
     
1791
                           PA0296_tr
                           translation of PA0296
                           458
                           95.85
                           98.68
                           0
                           2300.0
     

     
     
62673
                           gb|AAG03685.1|AE004467_7
                           (AE004467) probable glutamine synthetase [Pseudomonas aeruginosa PAO1]
                           458
                           95.85
                           98.68
                           0
                           2300.0
     

     
     
62674
                           gb|AAN70749.1|AE016718_8
                           (AE016793) glutamine synthetase, putative [Pseudomonas putida KT2440]
                           458
                           89.51
                           94.54
                           0
                           2168.0
     

     






      

          
GC ORFID: 43997How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2040Glimmer Score: 

          
Start: 1550399Stop: 1551775

          
Length: 1377


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2040 translation of PA2040Homolog Bit Score 919.0

          
Other Homologs:  t_PA0296 (ORF: BLASTX 890.0), psyr_15may02_Scaffold6_revised_gene6921 (ORF: BLASTX 825.0), t_PA5522 (ORF: BLASTX 377.0), t_PA1566 (ORF: BLASTX 367.0), t_PA2040 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGGTACCCCTGCGTGCCGTTCAGCTCACTGAGCCGAGCCTGTTCCTTCAGGAACATCCCGAGGTCCAG 
TTCGTCGACCTTCTGATTTCAGACATGAATGGCGTGGTTCGCGGCAAGCGAATCGAACGCAACAGTCTGCCC 
AAAGTCTTCGAAAAAGGTATCAACCTTCCCGCTTCCCTGTTCGCCCTCGATATCACCGGCTCCACCGTAGAG 
AGTACCGGCCTGGGTCTCGACATCGGCGACGCCGACCGGATCTGCTACCCGATCCCCGGCACCCTCTCCATG 
GAACCCTGGCAGAAGCGGCCAACCGCGCAACTGCTGATGACCATGCATGAGCTGGAAGGTGATCCCTTCTTC 
GCCGACCCCCGCGAAGTCCTGCGCCAGGTGGTGGCCAGGTTCACCGAGATGGAACTGACCATCGTCGCCGCC 
TTCGAGCTGGAGTTCTACCTGATCGACCAGGAGAACGTGAACGGCCGGCCGCAGCCGCCGCGCTCGCCGATC 
TCCGGCAAACGTCCGCAGTCGGTGCAGGTGTATTCCATCGACGACCTCGACGAATACGTCGAGTGCCTCCAG 
GACATCATCGACGGCGCCCGCGCCCAGGGCATCCCGGCCGACGCCATCGTCGCCGAATCCGCACCGGCGCAG 
TTCGAGGTCAACCTGCATCACGTCGCCGACCCGATGAAGGCCTGCGACTATGCGGTGCTGCTCAAGCGCCTG 
ATCAAGAACATCGCCTACGACCACGAGATGGACACCACCTTCATGGCCAAGCCCTATCCGGGCCAGGCTGGG 
AACGGACTGCACGTGCACATCTCGCTGCTCGACAAGCACGGCAACAACATCTTCACCGGCGAGGATCCCGAG 
CAGAACGCCGCATTGCGCCATGCGATCGGCGGCGTGCTCGAGACCCTGCCGGCCTCCATGGCCTTCCTCTGC 
CCGAACGTCAACTCCTACCGCCGCTTCGGTTCGCAGTTCTACGTGCCGAACGCGCCGAGCTGGGGCCTGGAC 
AACCGCACCGTGGCCCTGCGCGTGCCCACCGGCAGCCCGGACGCGGTACGCCTGGAACATCGCGTCGCCGGC 
GCCGACGCCAACCCCTACCTGCTGCTGGCCTCGGTGCTGGCCGGGGTACACCACGGCCTGACCAACCAGGTC 
GAGCCGGGCGCGCCGATCGAAGGCAACTCCTACGAGCAGTTGGAGCCGAGCCTGCCGAACAACCTGCGCGAC 
GCCCTGCGTGAACTGGACGACAGCGAGATCCTGGCGAAGTACATCGATCCGAAGTACATCGACATCTTCGTC 
GCCTGCAAGGAAAGCGAGCTGGAGGAGTTCGAGTACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCAC 
ACCGTGTGA