ViewGene
Locus Name:
GeneID:
Location:

GeneID:994
TrivialName: PA14_44240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44240
PAO1 Ortholog Locus:PA1566
Sequence Length:1365
Protein Length:454
Start: 3935985
Stop: 3937349
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04955.1|AE004585_3 (AE004585) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 450/454 (99%), Positives = 452/454 (99%)
Sequence:
ATGAACTTCGCCTGCGAACAAGAAGCCCAGGATTTCCTGGCCGCCAATCCCGATATCGAACTCTTCGAACTG
TTCATCCTCGACGCCAATGGCGTGCCACGCGGCAAACTGCTGCACCGCGACGAACTGCTGGCCGTCTACCGC
AGCGGCCGGCCGCTGCCGAGCACCATCCTGGGGCTGACAATGAACGGCGAGGATGTCGAGGACTCCGGCCTG
GTCTGGGACGTCGGCGACATCGACTGCCGCGCCTACCCCCTGTCCGGCAGCCTGGTGCGCCTGCCCTGGCGG
CAGATCCCGACGGCGGCGGTGCAGGTCAGCATGCACCCGAGCGAGGGCCTGCCCGCCAGCGTCGCCGATCCG
CGCCACCTCCTGGTGCGCACCATCGACGCGCTGAAGAGCGAGGGCTATCACCCGGTGATGGCCGCCGAGCTG
GAGTTCTACCTGCTCGACCGTCAGCGCGACGCCAACGGCCGCCCGCAACCGGCTCGCGACGCCGATGGCGGC
CGCCCGCGGGCGACCCAGGTCTACGGCCTGCGCGAACTGGAGCAGATCGAGCCGTTCCTCGCCGACCTCTAT
GCCGCATGCAAGGCGCAGGGCCTGCCGGCACGCACGGCGATCTCGGAATACGCCCCGGGCCAGGTGGAAATC
ACCCTCGACCACGGCGACGCCCTAGTCGCCATGGACCAGGCGATCCGCTACAAGCGCCTGGTCAAGGGCGTC
GCGCACAAGCACGGAATGCTCGCCTGCTTCATGGCCAAGCCGTTCGACGACCTGGCCGGCACCGGCATGCAC
CTGCACGTCAGCCTGGCCGACGAACAAGGCCACAACCTGTTCGCCAGCGACGACCCCGCCGGCACGCCGTTG
CTGCGCCAGGCGGTCGGCGGCATGCTCGCCAGCCTGCTCGACTCGCTCCTGCTGTTCTGTCCCAACGCCAAC
TCCTACCGGCGCTTCCAGGCCAACAGCTACGCGCCGCTGGCGCAGACCTGGGGCGTCGACAACCGCACCGTG
AGCCTGCGCGTGCCGGGCGGCCCGGCCTCCAGTCGGCATATCGAGCACCGCATCTGCGGCGCCGACGCCAAC
CCCTACCTGGCCGCCGCGGCGCTTCTCGCCGGGGTCCACCGCGGCATCCGCGAAGGCATCGATCCGGGCGCG
CCGGTGGAGGGCAATGGCTACGCCCAGGCCGGCAAGCGCCTGCCCACCGACTGGCTGACCGCCCTCGACGCC
CTGCAAGGCTCCGAGTGGGCGCGGGAAGCCTTCGGCGAGCCGTTCCTCGGCGTCTACCTGGCGGTGAAGCGC
GCGGAATACCGGCAATTCATGGGCGAGGTCGGCGAGCAGGACTGGCGCTGGTACCTGACCCAGGCCTGA
Translation:
MNFACEQEAQDFLAANPDIELFELFILDANGVPRGKLLHRDELLAVYRSGRPLPSTILGLTMNGEDVEDSGL
VWDVGDIDCRAYPLSGSLVRLPWRQIPTAAVQVSMHPSEGLPASVADPRHLLVRTIDALKSEGYHPVMAAEL
EFYLLDRQRDANGRPQPARDADGGRPRATQVYGLRELEQIEPFLADLYAACKAQGLPARTAISEYAPGQVEI
TLDHGDALVAMDQAIRYKRLVKGVAHKHGMLACFMAKPFDDLAGTGMHLHVSLADEQGHNLFASDDPAGTPL
LRQAVGGMLASLLDSLLLFCPNANSYRRFQANSYAPLAQTWGVDNRTVSLRVPGGPASSRHIEHRICGADAN
PYLAAAALLAGVHRGIREGIDPGAPVEGNGYAQAGKRLPTDWLTALDALQGSEWAREAFGEPFLGVYLAVKR
AEYRQFMGEVGEQDWRWYLTQA*
AnnotationID:1003GeneID:994
AnnotatorUID: diggins
Modification Date/Time: 2005-02-25 12:12:57
Gene Name:
Confidence Code:3
GeneProduct:putative glutamine synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00120
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70120.1|AE016652_1 (AE016791) glutamine synthetase, putative [Pseudomonas putida
           KT2440]
          Length = 454

 Score =  794 bits (2051), Expect = 0.0
 Identities = 385/454 (84%), Positives = 418/454 (92%)

>gb|AAK25100.1| (AE005977) glutamine synthetase family protein [Caulobacter
           crescentus CB15]
          Length = 460

 Score =  437 bits (1125), Expect = e-122
 Identities = 229/453 (50%), Positives = 292/453 (64%), Gaps = 6/453 (1%)

>dbj|BAB51646.1| (AP003005) probable glutamine synthetase [Mesorhizobium loti]
          Length = 466

 Score =  418 bits (1074), Expect = e-116
 Identities = 217/450 (48%), Positives = 302/450 (67%), Gaps = 8/450 (1%)








 
Structural Features:
pfam00120, Gln-synt_C, Glutamine synthetase, catalytic domain.
CD-Length = 260 residues,  99.6% aligned
Score =  265 bits (680), Expect = 5e-72
Identities = 103/262 (39%), Positives = 133/262 (50%), Gaps = 6/262 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1991




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19133




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37242




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:994_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:994
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1786
                           PA1566_tr
                           translation of PA1566
                           454
                           99.11
                           99.55
                           0
                           2375.0
     

     
     
62659
                           gb|AAG04955.1|AE004585_3
                           (AE004585) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           454
                           99.11
                           99.55
                           0
                           2375.0
     

     
     
62660
                           gb|AAN70120.1|AE016652_1
                           (AE016791) glutamine synthetase, putative [Pseudomonas putida KT2440]
                           454
                           84.80
                           92.07
                           0
                           2051.0
     

     
     
1787
                           PA5522_tr
                           translation of PA5522
                           455
                           75.60
                           87.03
                           1
                           1849.0
     

     
     
62661
                           gb|AAG08907.1|AE004964_10
                           (AE004964) probable glutamine synthetase [Pseudomonas aeruginosa PAO1]
                           455
                           75.60
                           87.03
                           1
                           1849.0
     

     






      

          
GC ORFID: 43996How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1566Glimmer Score: 

          
Start: 1017924Stop: 1016560

          
Length: 1365


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1566 translation of PA1566Homolog Bit Score 919.0

          
Other Homologs:  t_PA5522 (ORF: BLASTX 716.0), t_PA0296 (ORF: BLASTX 376.0), psyr_15may02_Scaffold6_revised_gene6921 (ORF: BLASTX 369.0), t_PA2040 (ORF: BLASTX 368.0), t_PA1566 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACTTCGCCTGCGAACAAGAAGCCCAGGATTTCCTGGCCGCCAATCCCGATATCGAACTCTTCGAACTG 
TTCATCCTCGACGCCAATGGCGTGCCACGCGGCAAACTGCTGCACCGCGACGAACTGCTGGCCGTCTACCGC 
AGCGGCCGGCCGCTGCCGAGCACCATCCTGGGGCTGACAATGAACGGCGAGGATGTCGAGGACTCCGGCCTG 
GTCTGGGACGTCGGCGACATCGACTGCCGCGCCTACCCCCTGTCCGGCAGCCTGGTGCGCCTGCCCTGGCGG 
CAGATCCCGACGGCGGCGGTGCAGGTCAGCATGCACCCGAGCGAGGGCCTGCCCGCCAGCGTCGCCGATCCG 
CGCCACCTCCTGGTGCGCACCATCGACGCGCTGAAGAGCGAGGGCTATCACCCGGTGATGGCCGCCGAGCTG 
GAGTTCTACCTGCTCGACCGTCAGCGCGACGCCAACGGCCGCCCGCAACCGGCTCGCGACGCCGATGGCGGC 
CGCCCGCGGGCGACCCAGGTCTACGGCCTGCGCGAACTGGAGCAGATCGAGCCGTTCCTCGCCGACCTCTAT 
GCCGCATGCAAGGCGCAGGGCCTGCCGGCACGCACGGCGATCTCGGAATACGCCCCGGGCCAGGTGGAAATC 
ACCCTCGACCACGGCGACGCCCTAGTCGCCATGGACCAGGCGATCCGCTACAAGCGCCTGGTCAAGGGCGTC 
GCGCACAAGCACGGAATGCTCGCCTGCTTCATGGCCAAGCCGTTCGACGACCTGGCCGGCACCGGCATGCAC 
CTGCACGTCAGCCTGGCCGACGAACAAGGCCACAACCTGTTCGCCAGCGACGACCCCGCCGGCACGCCGTTG 
CTGCGCCAGGCGGTCGGCGGCATGCTCGCCAGCCTGCTCGACTCGCTCCTGCTGTTCTGTCCCAACGCCAAC 
TCCTACCGGCGCTTCCAGGCCAACAGCTACGCGCCGCTGGCGCAGACCTGGGGCGTCGACAACCGCACCGTG 
AGCCTGCGCGTGCCGGGCGGCCCGGCCTCCAGTCGGCATATCGAGCACCGCATCTGCGGCGCCGACGCCAAC 
CCCTACCTGGCCGCCGCGGCGCTTCTCGCCGGGGTCCACCGCGGCATCCGCGAAGGCATCGATCCGGGCGCG 
CCGGTGGAGGGCAATGGCTACGCCCAGGCCGGCAAGCGCCTGCCCACCGACTGGCTGACCGCCCTCGACGCC 
CTGCAAGGCTCCGAGTGGGCGCGGGAAGCCTTCGGCGAGCCGTTCCTCGGCGTCTACCTGGCGGTGAAGCGC 
GCGGAATACCGGCAATTCATGGGCGAGGTCGGCGAGCAGGACTGGCGCTGGTACCTGACCCAGGCCTGA