ViewGene
Locus Name:
GeneID:
Location:

GeneID:986
TrivialName: PA14_71650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71650
PAO1 Ortholog Locus:PA5429
Sequence Length:1425
Protein Length:474
Start: 6382183
Stop: 6383607
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08814.1|AE004955_8 (AE004955) aspartate ammonia-lyase [Pseudomonas aeruginosa PAO1]
Identities = 474/474 (100%), Positives = 474/474 (100%)
Sequence:
ATGTCCCCCGTTGCATCTTCCCGTATCGAGAAAGACCTGCTCGGCACCCTCGAAGTGCCCGCTGACGCCTAC
TACGGCATCCAGACCCTGCGCGCCGTGAACAACTTCCGCCTCTCCGGGGTGCCGCTGTCGCACTACCCGAAG
CTGGTCGTTGCCCTGGCCATGGTCAAGCAGGCCGCCGCCGACGCCAACCGTCAGCTCGGCCATCTCCCGGAA
GACAAGCACGCCGCCATCAGCGAGGCCTGCGCCCGCCTGATCCGCGGCGATTTCCACGAGCAGTTCGTGGTG
GACATGATCCAGGGCGGCGCCGGTACCTCCACCAACATGAACGCGAACGAAGTGATCGCGAACATCGCGCTG
GAAGCCATGGGCCATACCAAGGGTGAGTACAAGTACCTGCACCCGAACAACGATGTGAACATGGCGCAGTCG
ACCAACGACGCCTACCCGACCGCCATCCGCCTGGGCCTGCTGCTCGGCCACGACACCCTGCTGGCCAGCCTC
GACAGCCTGATCCAGGCCTTCGCCGCCAAGGGCGTCGAATTCGCCGGCGTACTGAAGATGGGCCGCACCCAA
TTGCAGGACGCCGTGCCGATGACCCTCGGCCAGGAATTCCACGCTTTCGCCACCACCCTCGGCGAAGACCTC
GATCGCCTGCGTCGCCTGGCGCCGGAGCTGCTCACCGAAGTGAACCTCGGCGGCACCGCCATCGGCACCGGC
ATCAACGCCGACCCCGGCTACCAGAAGCTCGCCGTCGAGCGCCTGGCCGCCATCAGCGGGCAGCCGCTGAAA
CCGGCCGCCGACCTGATCGAAGCCACCTCCGACATGGGCGCCTTCGTGCTGTTCTCCGGCATGCTCAAGCGC
ACCGCGGTCAAGCTGTCGAAGATCTGCAACGACCTGCGCCTGCTCTCCAGCGGCCCGCGCACCGGCATCAAC
GAGATCAACCTGCCGCCGCGCCAGCCGGGCAGTTCGATCATGCCGGGCAAGGTCAACCCGGTGATCCCGGAA
GCGGTCAACCAGGTCGCCTTCGAAGTGATCGGCAACGACCTCGCCCTGACCCTGGCGGCCGAAGGCGGCCAG
TTGCAACTGAACGTGATGGAGCCGCTGATCGCCTACAAGATCTTCGACTCGATCCGCCTGCTGCAACGCGCC
ATGGACATGCTCCGCGAGCACTGCATCACCGGCATCACCGCCAACGTCGAGCGCTGCCACGAACTGGTCGAG
CACAGCATCGGCCTGGTCACCGCGCTGAACCCGTACATCGGCTACGAGAACTCCACGCGGATCGCCAAGACC
GCCCTGGAGAGCGGCCGCGGCGTACTGGAACTGGTACGCGAGGAAAAGCTGCTGGACGAAGCCACCCTGGCC
GACATCCTGCTGCCGGAAAACATGATCGCGCCGCGCCTGATTCCGCTGCGCGCCTGA
Translation:
MSPVASSRIEKDLLGTLEVPADAYYGIQTLRAVNNFRLSGVPLSHYPKLVVALAMVKQAAADANRQLGHLPE
DKHAAISEACARLIRGDFHEQFVVDMIQGGAGTSTNMNANEVIANIALEAMGHTKGEYKYLHPNNDVNMAQS
TNDAYPTAIRLGLLLGHDTLLASLDSLIQAFAAKGVEFAGVLKMGRTQLQDAVPMTLGQEFHAFATTLGEDL
DRLRRLAPELLTEVNLGGTAIGTGINADPGYQKLAVERLAAISGQPLKPAADLIEATSDMGAFVLFSGMLKR
TAVKLSKICNDLRLLSSGPRTGINEINLPPRQPGSSIMPGKVNPVIPEAVNQVAFEVIGNDLALTLAAEGGQ
LQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCITGITANVERCHELVEHSIGLVTALNPYIGYENSTRIAKT
ALESGRGVLELVREEKLLDEATLADILLPENMIAPRLIPLRA*
AnnotationID:995GeneID:986
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 15:03:33
Gene Name:aspA
Confidence Code:2
GeneProduct:aspartate ammonia-lyase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:aspartase
Functional Category Confidence Code:5
COGs:COG1027,COG0114,pfam00206
Secondary Functional Category(ies):
EC Number:4.3.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70903.1|AE016734_7 (AE016794) aspartate ammonia-lyase [Pseudomonas putida KT2440]
          Length = 478

 Score =  831 bits (2147), Expect = 0.0
 Identities = 425/474 (89%), Positives = 444/474 (93%)

>gb|AAO58918.1| (AE016876) aspartate ammonia-lyase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 474

 Score =  828 bits (2138), Expect = 0.0
 Identities = 429/474 (90%), Positives = 444/474 (93%)

>emb|CAA05764.1| (AJ002933) aspartate ammonia-lyase [Wolinella succinogenes]
          Length = 471

 Score =  560 bits (1444), Expect = e-159
 Identities = 286/469 (60%), Positives = 352/469 (75%), Gaps = 1/469 (0%)











 
Structural Features:
COG1027, AspA, Aspartate ammonia-lyase [Amino acid transport and metabolism]
CD-Length = 471 residues,  98.3% aligned
Score =  746 bits (1927), Expect = 0.0
Identities = 307/464 (66%), Positives = 360/464 (77%), Gaps = 1/464 (0%)

COG0114, FumC, Fumarase [Energy production and conversion]
CD-Length = 462 residues, 100.0% aligned
Score =  506 bits (1305), Expect = 2e-144
Identities = 214/465 (46%), Positives = 288/465 (61%), Gaps = 4/465 (0%)

pfam00206, Lyase_1, Lyase.
CD-Length = 312 residues, 100.0% aligned
Score =  328 bits (843), Expect = 6e-91
Identities = 152/335 (45%), Positives = 191/335 (57%), Gaps = 25/335 (7%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1975




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19117




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34681




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:986_cdd.html


    		




 
URL:

















    ReferenceID:35631




  
Author/Investigator(s):
        Asai Y, Inui M, Vertes A, Kobayashi M, Yukawa H.





  
Title:
        Cloning and sequence determination of the aspartase-encoding gene from Brevibacterium flavum MJ233.




  
PubMed:
        7789816


MedLine:
        




    
Source:
Gene. 1995 May 26;158(1):87-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7789816

















    ReferenceID:35632




  
Author/Investigator(s):
        Whitby PW, Landon M, Coleman G.





  
Title:
        The cloning and nucleotide sequence of the serine protease gene (aspA) of Aeromonas salmonicida ssp. salmonicida.




  
PubMed:
        1468618


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1992 Nov 15;78(1):65-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1468618

















    ReferenceID:35633




  
Author/Investigator(s):
        Takagi JS, Tokushige M, Shimura Y.





  
Title:
        Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.




  
PubMed:
        3096982


MedLine:
        




    
Source:
J Biochem (Tokyo). 1986 Sep;100(3):697-705.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3096982

















    ReferenceID:35634




  
Author/Investigator(s):
        Takagi JS, Ida N, Tokushige M, Sakamoto H, Shimura Y.





  
Title:
        Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.




  
PubMed:
        2987841


MedLine:
        




    
Source:
Nucleic Acids Res. 1985 Mar 25;13(6):2063-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2987841









           
Homologs By Global Alignment 

           

       



     
Gene ID:986
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1763
                           PA5429_tr
                           translation of PA5429
                           474
                           99.99
                           99.99
                           0
                           2380.0
     

     
     
61827
                           gb|AAG08814.1|AE004955_8
                           (AE004955) aspartate ammonia-lyase [Pseudomonas aeruginosa PAO1]
                           474
                           99.99
                           99.99
                           0
                           2380.0
     

     
     
61831
                           gb|AAO58918.1|
                           (AE016876) aspartate ammonia-lyase [Pseudomonas syringae pv. tomato str. DC3000]
                           474
                           90.50
                           93.67
                           0
                           2138.0
     

     
     
61829
                           emb|CAA28037.1|
                           (X04441) unnamed protein product [Pseudomonas fluorescens]
                           478
                           89.53
                           92.67
                           4
                           2141.0
     

     
     
61830
                           dbj|BAA00062.1|
                           (D00100) aspartase [Pseudomonas fluorescens]
                           478
                           89.53
                           92.67
                           4
                           2141.0
     

     






      

          
GC ORFID: 43988How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5429Glimmer Score: 

          
Start: 5645872Stop: 5647296

          
Length: 1425


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5429 translation of PA5429Homolog Bit Score 921.0

          
Other Homologs:  t_PA0854 (ORF: BLASTX 347.0), t_PA4470 (ORF: BLASTX 317.0), t_PA5429 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCCCGTTGCATCTTCCCGTATCGAGAAAGACCTGCTCGGCACCCTCGAAGTGCCCGCTGACGCCTAC 
TACGGCATCCAGACCCTGCGCGCCGTGAACAACTTCCGCCTCTCCGGGGTGCCGCTGTCGCACTACCCGAAG 
CTGGTCGTTGCCCTGGCCATGGTCAAGCAGGCCGCCGCCGACGCCAACCGTCAGCTCGGCCATCTCCCGGAA 
GACAAGCACGCCGCCATCAGCGAGGCCTGCGCCCGCCTGATCCGCGGCGATTTCCACGAGCAGTTCGTGGTG 
GACATGATCCAGGGCGGCGCCGGTACCTCCACCAACATGAACGCGAACGAAGTGATCGCGAACATCGCGCTG 
GAAGCCATGGGCCATACCAAGGGTGAGTACAAGTACCTGCACCCGAACAACGATGTGAACATGGCGCAGTCG 
ACCAACGACGCCTACCCGACCGCCATCCGCCTGGGCCTGCTGCTCGGCCACGACACCCTGCTGGCCAGCCTC 
GACAGCCTGATCCAGGCCTTCGCCGCCAAGGGCGTCGAATTCGCCGGCGTACTGAAGATGGGCCGCACCCAA 
TTGCAGGACGCCGTGCCGATGACCCTCGGCCAGGAATTCCACGCTTTCGCCACCACCCTCGGCGAAGACCTC 
GATCGCCTGCGTCGCCTGGCGCCGGAGCTGCTCACCGAAGTGAACCTCGGCGGCACCGCCATCGGCACCGGC 
ATCAACGCCGACCCCGGCTACCAGAAGCTCGCCGTCGAGCGCCTGGCCGCCATCAGCGGGCAGCCGCTGAAA 
CCGGCCGCCGACCTGATCGAAGCCACCTCCGACATGGGCGCCTTCGTGCTGTTCTCCGGCATGCTCAAGCGC 
ACCGCGGTCAAGCTGTCGAAGATCTGCAACGACCTGCGCCTGCTCTCCAGCGGCCCGCGCACCGGCATCAAC 
GAGATCAACCTGCCGCCGCGCCAGCCGGGCAGTTCGATCATGCCGGGCAAGGTCAACCCGGTGATCCCGGAA 
GCGGTCAACCAGGTCGCCTTCGAAGTGATCGGCAACGACCTCGCCCTGACCCTGGCGGCCGAAGGCGGCCAG 
TTGCAACTGAACGTGATGGAGCCGCTGATCGCCTACAAGATCTTCGACTCGATCCGCCTGCTGCAACGCGCC 
ATGGACATGCTCCGCGAGCACTGCATCACCGGCATCACCGCCAACGTCGAGCGCTGCCACGAACTGGTCGAG 
CACAGCATCGGCCTGGTCACCGCGCTGAACCCGTACATCGGCTACGAGAACTCCACGCGGATCGCCAAGACC 
GCCCTGGAGAGCGGCCGCGGCGTACTGGAACTGGTACGCGAGGAAAAGCTGCTGGACGAAGCCACCCTGGCC 
GACATCCTGCTGCCGGAAAACATGATCGCGCCGCGCCTGATTCCGCTGCGCGCCTGA