ViewGene
Locus Name:
GeneID:
Location:

GeneID:984
TrivialName: PA14_11770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11770
PAO1 Ortholog Locus:PA4024
Sequence Length:1395
Protein Length:464
Start: 1020741
Stop: 1019347
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07411.1|AE004819_4 (AE004819) ethanolamine ammonia-lyase large subunit [Pseudomonas aeruginosa PAO1]
Identities = 464/464 (100%), Positives = 464/464 (100%)
Sequence:
ATGGCACGTTTCACCCATAGCGTCGGCGGCGAGACCTACCGCTTCGACAGCCTCAAGGACGTGATGGCCAAG
GCCAGCCCCGCGCGCTCCGGCGATTTCCTCGCCGGCGTCGCGGCGAGCAACGACGGCGAGCGGGTCGCCGCG
CAGATGGCCCTGGCCGACATCCCGCTCAAACACTTCCTCGACGAGGCGCTGATTCCCTACGAGGACGACGAG
GTCACCCGCCTGATCATCGACACCCACCAGCGCGACGCCTTCGCCCCGGTGTCCCATCTCACCGTCGGCGGC
TTCCGCGACTGGCTGCTCGGCGACGCCGCCGACGAGGCCAGCCTGCGCGCCCTGGCTCCGGGCCTGACGCCG
GAAATGGCAGCGGCGGTATCGAAGATCATGCGTGTCCAGGACCTGGTCCTGGTGGCGCAGAAGATCCGCGTG
GTCACCCGCTTCCGCAACACCCTCGGCCTTCGCGGCCGACTCTCCACCCGCCTGCAACCGAACCACCCGACC
GACGACCCGGCCGGAATCGCCGCGAGCATCCTCGACGGCCTGCTGTTCGGCAATGGCGACGCGATGCTCGGG
ATCAACCCGGCCACCGACAGCATGGCCTCGATCTGCGCCCTGCTGGAGATGCTCGACGCGATCATCCAGCGC
TACGAGATTCCCACCCAGGCCTGCGTGCTGACCCACGTCACCAGCTCCATCGAGGCGATCAACCGCGGCGTG
CCGCTGGACCTGGTGTTCCAGTCGATCGCCGGCACCGAGGCGGCCAATGCCAGCTTCGGCATCAGCCTGAAG
ATCCTCCAGGAAGGCTACGAGGCCGGGCTCAGCCAGAAGCGCGGCACCCTCGGCAACAACCTGATGTACTTC
GAGACCGGCCAGGGCAGCGCGCTCTCGGCCAACGCCCATCACGGCGTCGACCAGCAGACCTGCGAGACCCGC
GCCTATGCGGTGGCTCGTCACTTCAAGCCGTTCCTGGTGAACACCGTGGTCGGCTTCATCGGTCCCGAGTAC
CTATACAACGGCAAGCAGATCATCCGCGCCGGCCTGGAAGACCACTTCTGCGGCAAGCTGCTCGGCGTGCCG
ATGGGTTGCGACATCTGCTACACCAACCATGCCGAGGCCGACCAGGACGACATGGACATGCTCCTGACCCTG
CTGGGCGTCGCCGGGATCAACTTCATCATGGGCATTCCCGGCTCCGACGACGTGATGCTCAACTACCAGACC
ACCTCCTTCCACGACGCCCTCTATGCGCGCCAGACGCTGGGCCTGAAGCCGGCGCCGGAATTCGAGGACTGG
TTGCAGCGGATGGGAATCTTCACCCAGGCCGACGGGCGCATCCGCTTCGGCGACGAGCTACCGCCGGCGTTC
CGCCAGGCCCTGGCGCAACTGGCCTGA
Translation:
MARFTHSVGGETYRFDSLKDVMAKASPARSGDFLAGVAASNDGERVAAQMALADIPLKHFLDEALIPYEDDE
VTRLIIDTHQRDAFAPVSHLTVGGFRDWLLGDAADEASLRALAPGLTPEMAAAVSKIMRVQDLVLVAQKIRV
VTRFRNTLGLRGRLSTRLQPNHPTDDPAGIAASILDGLLFGNGDAMLGINPATDSMASICALLEMLDAIIQR
YEIPTQACVLTHVTSSIEAINRGVPLDLVFQSIAGTEAANASFGISLKILQEGYEAGLSQKRGTLGNNLMYF
ETGQGSALSANAHHGVDQQTCETRAYAVARHFKPFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVP
MGCDICYTNHAEADQDDMDMLLTLLGVAGINFIMGIPGSDDVMLNYQTTSFHDALYARQTLGLKPAPEFEDW
LQRMGIFTQADGRIRFGDELPPAFRQALAQLA*
AnnotationID:993GeneID:984
AnnotatorUID: danlee
Modification Date/Time: 2006-01-27 16:04:00
Gene Name:eutB
Confidence Code:2
GeneProduct:ethanolamine ammonia-lyase large subunit, EutB
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4303,pfam06751
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gb|AAC37136.1|

# Aligned_sequences: 2
# 1: EutB_pa
# 2: AAC37136.1
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 480
# Identity:     319/480 (66.5%)
# Similarity:   372/480 (77.5%)
# Gaps:          22/480 ( 4.6%)
# Score: 1593.5

gb|NP_461393.1|
# Aligned_sequences: 2
# 1: EutB_pa
# 2: NP_461393.1
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 466
# Identity:     226/466 (48.5%)
# Similarity:   299/466 (64.2%)
# Gaps:          15/466 ( 3.2%)
# Score: 1117.5







 
Structural Features:
gnl|CDD|13550 COG4303, EutB, Ethanolamine ammonia-lyase, large subunit
[Amino acid transport and metabolism]
Length = 453
Score =  784 bits (2025), Expect = 0.0
Identities = 267/456 (58%), Positives = 320/456 (70%), Gaps = 3/456 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1971




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19113




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34391




  
Author/Investigator(s):
        De Mot R, Nagy I, Schoofs G, Vanderleyden J.





  
Title:
        Sequence of Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease.




  
PubMed:
        8069783


MedLine:
        94349216




    
Source:
Can J Microbiol. 1994 May;40(5):403-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=94349216&dopt=Citation

















    ReferenceID:34392




  
Author/Investigator(s):
        Roof DM, Roth JR.





  
Title:
        Functions required for vitamin B12-dependent ethanolamine utilization in Salmonella typhimurium.




  
PubMed:
        2656649


MedLine:
        




    
Source:
J Bacteriol. 1989 Jun;171(6):3316-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2656649









           
Homologs By Global Alignment 

           

       



     
Gene ID:984
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1758
                           PA4024_tr
                           translation of PA4024
                           464
                           99.99
                           99.99
                           0
                           2383.0
     

     
     
61785
                           gb|AAG07411.1|AE004819_4
                           (AE004819) ethanolamine ammonia-lyase large subunit [Pseudomonas aeruginosa PAO1]
                           464
                           99.99
                           99.99
                           0
                           2383.0
     

     
     
61786
                           gb|AAN66170.1|AE016244_11
                           (AE016776) ethanolamine ammonia-lyase, heavy subunit [Pseudomonas putida KT2440]
                           464
                           90.30
                           94.18
                           0
                           2164.0
     

     
     
61787
                           gb|AAO54269.1|
                           (AE016858) ethanolamine ammonia-lyase, heavy subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           464
                           88.79
                           94.61
                           0
                           2162.0
     

     
     
61788
                           emb|CAD16836.1|
                           (AL646073) PUTATIVE ETHANOLAMINE AMMONIA-LYASE HEAVY CHAIN PROTEIN [Ralstonia solanacearum]
                           464
                           80.17
                           91.37
                           0
                           1987.0
     

     






      

          
GC ORFID: 43986How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4024Glimmer Score: 

          
Start: 3933196Stop: 3934590

          
Length: 1395


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4024 translation of PA4024Homolog Bit Score 922.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3625 (ORF: BLASTX 845.0), t_PA4024 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACGTTTCACCCATAGCGTCGGCGGCGAGACCTACCGCTTCGACAGCCTCAAGGACGTGATGGCCAAG 
GCCAGCCCCGCGCGCTCCGGCGATTTCCTCGCCGGCGTCGCGGCGAGCAACGACGGCGAGCGGGTCGCCGCG 
CAGATGGCCCTGGCCGACATCCCGCTCAAACACTTCCTCGACGAGGCGCTGATTCCCTACGAGGACGACGAG 
GTCACCCGCCTGATCATCGACACCCACCAGCGCGACGCCTTCGCCCCGGTGTCCCATCTCACCGTCGGCGGC 
TTCCGCGACTGGCTGCTCGGCGACGCCGCCGACGAGGCCAGCCTGCGCGCCCTGGCTCCGGGCCTGACGCCG 
GAAATGGCAGCGGCGGTATCGAAGATCATGCGTGTCCAGGACCTGGTCCTGGTGGCGCAGAAGATCCGCGTG 
GTCACCCGCTTCCGCAACACCCTCGGCCTTCGCGGCCGACTCTCCACCCGCCTGCAACCGAACCACCCGACC 
GACGACCCGGCCGGAATCGCCGCGAGCATCCTCGACGGCCTGCTGTTCGGCAATGGCGACGCGATGCTCGGG 
ATCAACCCGGCCACCGACAGCATGGCCTCGATCTGCGCCCTGCTGGAGATGCTCGACGCGATCATCCAGCGC 
TACGAGATTCCCACCCAGGCCTGCGTGCTGACCCACGTCACCAGCTCCATCGAGGCGATCAACCGCGGCGTG 
CCGCTGGACCTGGTGTTCCAGTCGATCGCCGGCACCGAGGCGGCCAATGCCAGCTTCGGCATCAGCCTGAAG 
ATCCTCCAGGAAGGCTACGAGGCCGGGCTCAGCCAGAAGCGCGGCACCCTCGGCAACAACCTGATGTACTTC 
GAGACCGGCCAGGGCAGCGCGCTCTCGGCCAACGCCCATCACGGCGTCGACCAGCAGACCTGCGAGACCCGC 
GCCTATGCGGTGGCTCGTCACTTCAAGCCGTTCCTGGTGAACACCGTGGTCGGCTTCATCGGTCCCGAGTAC 
CTATACAACGGCAAGCAGATCATCCGCGCCGGCCTGGAAGACCACTTCTGCGGCAAGCTGCTCGGCGTGCCG 
ATGGGTTGCGACATCTGCTACACCAACCATGCCGAGGCCGACCAGGACGACATGGACATGCTCCTGACCCTG 
CTGGGCGTCGCCGGGATCAACTTCATCATGGGCATTCCCGGCTCCGACGACGTGATGCTCAACTACCAGACC 
ACCTCCTTCCACGACGCCCTCTATGCGCGCCAGACGCTGGGCCTGAAGCCGGCGCCGGAATTCGAGGACTGG 
TTGCAGCGGATGGGAATCTTCACCCAGGCCGACGGGCGCATCCGCTTCGGCGACGAGCTACCGCCGGCGTTC 
CGCCAGGCCCTGGCGCAACTGGCCTGA