ViewGene
Locus Name:
GeneID:
Location:

GeneID:98
TrivialName: PA14_18250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18250
PAO1 Ortholog Locus:PA3562
Sequence Length:2871
Protein Length:956
Start: 1563719
Stop: 1566589
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06950.1|AE004777_3 (AE004777) probable phosphotransferase system enzyme I [Pseudomonas aeruginosa PAO1]
Identities = 953/956 (99%), Positives = 953/956 (99%)
Sequence:
ATGCTCGAACTCGATACCCGGCAGATCCGCATGGGCCAGCGCGCCGCGGACAAGGCCGAAGCCCTGCGCCTG
CTCGGCGCGGCGCTGGTCGCCGACGGCCTGGCCGCGCCCGGCTATGCCGAGGGCCTGAAGGCGCGCGAGGCA
CAAGGCTCCACCTACCTCGGCCAGGGCATCGCCATACCCCATGGGACCCCGGATACCCGCGAGCTGGTCTTC
AGCACCGGTGTGCGCCTGCTGCAATTCCCCGAGGGAGTGGACTGGGGCGATGGCCAGCAGGTCTACCTGGCC
ATCGGCATCGCGGCGAAGTCCGACGAACACCTGCAGCTCCTGCAATTGCTCACCCGCGCCCTCGGCGAGGCC
GACCTGGGACCGGCGCTGAGCGCGGCCGCCAGCGCCGAGGAAGTCCTCGGCCTGTTGCAGGGCGCGCCGCAG
GAACTGGCGCTGGACGCACAACTGGTCGGCCTCGGCCAGAACGCCGAGGACCTCGACGAACTGGCCTGGCTC
GGCGCGCGCCTGTTGAAGAAGGCCGGCTGCGTGGAGAACGGCTTCGCCGCCGTGCTCCAGCAGACCGAACCG
TTGCCGCTGGGCGACGGGCTGTGCTGGTTGCACAGCGAACAATTGGTGAAGCGCCCCGGGCTGGCCTTCGTT
ACTCCGGCGCAACCGTTGCAGCACCAGGGGCAACCGGTCACCGGGCTGTTCTGCCTGGCCAGCCTGGGCGAA
GCGCACCAGGCGCTGCTCGAACGCCTGTGCGACCTGTTGCTGGAAGGGCGTGGCGCCGAGCTGGTGCGCGCC
ACCAGCAGCCGCAGCGTACTCGCGGCGCTCGGTGGCGAGCTGCCGCCGGACTGGCCCAGCGCGCGAGCGGTC
CTGGCCAACCCGCATGGCCTGCATGCCCGCCCGGCCCAGGCGCTGGCGCAGCTGGCCAAggGCTtCGCCGGC
GAgATCCGGGTACGCctggccGACAGCGAggcggcCCCCgtctcGgcGAAGAGCCTGAGCAAGCTGCTCGCC
CTCGGCGCGCGACGCGGCCAGACGCTGGAGTTCAGCGCCGAGCCGGCGATCGCCGAGGACGCGCTGCCAGCC
TTGCTGGCCGCGGTGCGCGAGGGGCTCGGCGAGGAGGTCGAAGCGCTGGCGGAGGAGGCCTTGCCCGATGCC
GTCGGCGAGGCGGAAGAGGATGCCCGGCCGGCGCCGCTGCGCGCCGGCGAGCGCCTGCAGGCGATCGCCGCG
TCGCCGGGCATCGCCAGTGGTCCGGCGCATGTCCAGGTGGCGCAGCGCTTCGAGTTCCAGCCGCGTGGCGAG
TCGCCGGCCCATGAACGCGAGCGCCTGCTGCGGGCCAAGCGGGCGGTGGACGAGGAGATCGTCGGCCTGGTC
GAACGCAGCACGGTGAAGGCGATCCGCGAGATTTTCGTCACCCACCGGGAGATGCTCGACGATCCGGAACTG
GCCGAGCAGGTGCAACTGCGCCTGAATCGCGGCGAAAGCGCCGAAGCGGCCTGGAGTCGGGTAGTGGAAGAC
AGCGCGGCGCAGCAGGAGGCGCTGCACGACACCTTGCTCGCCGAGCGCGCCGCCGACCTCCGCGACCTCGGC
CGGCGGGTGCTGGCCAGGCTCTGCGGGGTGGAGGCGCCGCGCGAGCCGGAGCAGCCGTACATCCTGGTGATG
GACGAGGTCGGCCCCTCCGATGTCGCCCGCCTCGACGCCCAGCGGGTCGCCGGCATCCTCACGGCGCGCGGC
GGCGCCACTTCGCACAGCGCGATCATCGCCCGCGCCCTCGGCATTCCGGCATTGGTCGGCGCCGGCGCGGCG
GTGCTCGGCCTGGAGCCGGGCACGGCACTGCTGCTGGATGGCGAACACGGCTGGCTGCAGGTGGCGCCGAGC
ACCGAACAGTTGCAGCAGGCCGCCGCCGAGCGCGACGCCCGCCAGCAGCGCCAGGCCCGCGCCGACGCGCAG
CGTCTGGAGCCTGCGCGGACCCGCGACGGCCACGCGGTGGAAGTCTGCGCCAACCTTGGCGACACCGCCGGC
GCCGCGCGCGCGGTGGAGCTGGGCGCCGAGGGCGTTGGCCTGCTGCGCACCGAGTTCGTCTTCATGAACAAC
GCCCGCGCGCCGGACCTGGCGACCCAGGAAGCGGAATACCGTCGGGTGCTCGACGCCCTCGACGGACGTCCG
CTGGTGGCGCGGACCCTGGATGTCGGCGGGGACAAGCCGCTGCCGTACTGGCCGATTTCCCACGAAGAGAAT
CCCTATCTGGGCCTGCGCGGTATCCGCTTGACCCTGCAGCGACCGCAGATCCTCGAGACCCAGTTGCGCGCG
CTGTTCCGCGCCGCCGGGGAGCGACCGCTGCGGGTGATGTTTCCGATGGTCGGCAGCCTCGACGAATGGCGC
CAGGCGCGTGACCTGGCGCTGCGCCTGCGCGAGGAAATCCCGCTGGCCGACCTGCAACTGGGGATCATGGTT
GAGGTGCCCTCGGCGGCGTTGCTCGCGCCGGTGCTGGCCCGCGAGGTGGATTTCTTCAGCGTCGGCACCAAC
GACCTGACCCAGTACACCCTGGCCATCGATCGCGGCCACCCGAGTCTTTCGGCCCAGGCCGACGGCCTGCAT
CCGGCGGTGCTGCAACTGATCGACATGACCGTCCGCGCCGCCCATGCCGAGGGCAAGTGGGTCGGCGTCTGC
GGCGAACTGGCGGCCGACCCGCTGGCGTTGCCGCTGCTGGTCGGCCTGGGAGTGGACGAGCTGAGCGTCTCG
GCGCGCAGCATCGCCCTGGTCAAGGCCGGCGTGCGCGAACTGCAGCTGGTCGCTGCGCGCGGCCTGGCCCGC
AAAGCCCTGGGCCTGGCGAGCGCCGCCGAGGTCCGGGCCCTGGTGGAAGCGGAGGTGCAGTAA
Translation:
MLELDTRQIRMGQRAADKAEALRLLGAALVADGLAAPGYAEGLKAREAQGSTYLGQGIAIPHGTPDTRELVF
STGVRLLQFPEGVDWGDGQQVYLAIGIAAKSDEHLQLLQLLTRALGEADLGPALSAAASAEEVLGLLQGAPQ
ELALDAQLVGLGQNAEDLDELAWLGARLLKKAGCVENGFAAVLQQTEPLPLGDGLCWLHSEQLVKRPGLAFV
TPAQPLQHQGQPVTGLFCLASLGEAHQALLERLCDLLLEGRGAELVRATSSRSVLAALGGELPPDWPSARAV
LANPHGLHARPAQALAQLAKGFAGEIRVRLADSEAAPVSAKSLSKLLALGARRGQTLEFSAEPAIAEDALPA
LLAAVREGLGEEVEALAEEALPDAVGEAEEDARPAPLRAGERLQAIAASPGIASGPAHVQVAQRFEFQPRGE
SPAHERERLLRAKRAVDEEIVGLVERSTVKAIREIFVTHREMLDDPELAEQVQLRLNRGESAEAAWSRVVED
SAAQQEALHDTLLAERAADLRDLGRRVLARLCGVEAPREPEQPYILVMDEVGPSDVARLDAQRVAGILTARG
GATSHSAIIARALGIPALVGAGAAVLGLEPGTALLLDGEHGWLQVAPSTEQLQQAAAERDARQQRQARADAQ
RLEPARTRDGHAVEVCANLGDTAGAARAVELGAEGVGLLRTEFVFMNNARAPDLATQEAEYRRVLDALDGRP
LVARTLDVGGDKPLPYWPISHEENPYLGLRGIRLTLQRPQILETQLRALFRAAGERPLRVMFPMVGSLDEWR
QARDLALRLREEIPLADLQLGIMVEVPSAALLAPVLAREVDFFSVGTNDLTQYTLAIDRGHPSLSAQADGLH
PAVLQLIDMTVRAAHAEGKWVGVCGELAADPLALPLLVGLGVDELSVSARSIALVKAGVRELQLVAARGLAR
KALGLASAAEVRALVEAEVQ*
AnnotationID:108GeneID:98
AnnotatorUID: diggins
Modification Date/Time: 2005-07-10 10:10:13
Gene Name:
Confidence Code:3
GeneProduct:putative phosphotransferase system, fructose-specific component
Cell Localization:(1) Cytoplasmic
Synonyms:fruB
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1080,COG3605
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.7.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66418.1|AE016269_5 (AE016777) phosphotransferase system, fructose-specific EI/HPr/EIIA
           components [Pseudomonas putida KT2440]
          Length = 950

 Score = 1318 bits (3410), Expect = 0.0
 Identities = 691/952 (72%), Positives = 786/952 (82%), Gaps = 4/952 (0%)

>emb|CAA85482.1| (Z37113) fructose phosphotransferase Multiphosphoryltransfer
           Protein MTP [Xanthomonas campestris]
          Length = 837

 Score =  494 bits (1272), Expect = e-138
 Identities = 330/840 (39%), Positives = 460/840 (54%), Gaps = 44/840 (5%)







 
Structural Features:
COG1080, PtsA, Phosphoenolpyruvate-protein kinase 
(PTS system EI component in bacteria) 
[Carbohydrate transport and metabolism]
CD-Length = 574 residues,  99.0% aligned
Score =  626 bits (1616), Expect = 8e-180
Identities = 279/568 (49%), Positives = 359/568 (63%), Gaps = 15/568 (2%)

COG3605, PtsP, Signal transduction protein containing GAF and PtsI domains 
[Signal transduction mechanisms]
CD-Length = 756 residues,  79.9% aligned
Score =  408 bits (1051), Expect = 3e-114
Identities = 232/607 (38%), Positives = 326/607 (53%), Gaps = 27/607 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:199




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17341




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31982




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:98_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:98
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
190
                           PA3562_tr
                           translation of PA3562
                           956
                           99.68
                           99.68
                           0
                           4738.0
     

     
     
14575
                           gb|AAG06950.1|AE004777_3
                           (AE004777) probable phosphotransferase system enzyme I [Pseudomonas aeruginosa PAO1]
                           956
                           99.68
                           99.68
                           0
                           4738.0
     

     
     
14576
                           gb|AAN66418.1|AE016269_5
                           (AE016777) phosphotransferase system, fructose-specific EI/HPr/EIIA components [Pseudomonas putida KT2440]
                           956
                           72.28
                           82.21
                           6
                           3412.0
     

     
     
14577
                           gb|AAO54488.1|
                           (AE016859) phosphoenolpyruvate-protein phosphotransferase,EI/HPr/EIIA components [Pseudomonas syringae pv. tomato str. DC3000]
                           958
                           71.50
                           81.73
                           5
                           3382.0
     

     
     
14579
                           emb|CAD16568.1|
                           (AL646072) PROBABLE MULTIPHOSPHORYL TRANSFER PROTEIN MTP [Ralstonia solanacearum]
                           996
                           39.65
                           49.49
                           192
                           1527.0
     

     






      

          
GC ORFID: 43100How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3562Glimmer Score: 

          
Start: 3390217Stop: 3387347

          
Length: 2871


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3562 translation of PA3562Homolog Bit Score 1829.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2494 (ORF: BLASTX 1317.0), t_PA0337 (ORF: BLASTX 320.0), psyr_15may02_Scaffold6_revised_gene6532 (ORF: BLASTX 319.0), t_PA3562 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGAACTCGATACCCGGCAGATCCGCATGGGCCAGCGCGCCGCGGACAAGGCCGAAGCCCTGCGCCTG 
CTCGGCGCGGCGCTGGTCGCCGACGGCCTGGCCGCGCCCGGCTATGCCGAGGGCCTGAAGGCGCGCGAGGCA 
CAAGGCTCCACCTACCTCGGCCAGGGCATCGCCATACCCCATGGGACCCCGGATACCCGCGAGCTGGTCTTC 
AGCACCGGTGTGCGCCTGCTGCAATTCCCCGAGGGAGTGGACTGGGGCGATGGCCAGCAGGTCTACCTGGCC 
ATCGGCATCGCGGCGAAGTCCGACGAACACCTGCAGCTCCTGCAATTGCTCACCCGCGCCCTCGGCGAGGCC 
GACCTGGGACCGGCGCTGAGCGCGGCCGCCAGCGCCGAGGAAGTCCTCGGCCTGTTGCAGGGCGCGCCGCAG 
GAACTGGCGCTGGACGCACAACTGGTCGGCCTCGGCCAGAACGCCGAGGACCTCGACGAACTGGCCTGGCTC 
GGCGCGCGCCTGTTGAAGAAGGCCGGCTGCGTGGAGAACGGCTTCGCCGCCGTGCTCCAGCAGACCGAACCG 
TTGCCGCTGGGCGACGGGCTGTGCTGGTTGCACAGCGAACAATTGGTGAAGCGCCCCGGGCTGGCCTTCGTT 
ACTCCGGCGCAACCGTTGCAGCACCAGGGGCAACCGGTCACCGGGCTGTTCTGCCTGGCCAGCCTGGGCGAA 
GCGCACCAGGCGCTGCTCGAACGCCTGTGCGACCTGTTGCTGGAAGGGCGTGGCGCCGAGCTGGTGCGCGCC 
ACCAGCAGCCGCAGCGTACTCGCGGCGCTCGGTGGCGAGCTGCCGCCGGACTGGCCCAGCGCGCGAGCGGTC 
CTGGCCAACCCGCATGGCCTGCATGCCCGCCCGGCCCAGGCGCTGGCGCAGCTGGCCAAggGCTtCGCCGGC 
GAgATCCGGGTACGCctggccGACAGCGAggcggcCCCCgtctcGgcGAAGAGCCTGAGCAAGCTGCTCGCC 
CTCGGCGCGCGACGCGGCCAGACGCTGGAGTTCAGCGCCGAGCCGGCGATCGCCGAGGACGCGCTGCCAGCC 
TTGCTGGCCGCGGTGCGCGAGGGGCTCGGCGAGGAGGTCGAAGCGCTGGCGGAGGAGGCCTTGCCCGATGCC 
GTCGGCGAGGCGGAAGAGGATGCCCGGCCGGCGCCGCTGCGCGCCGGCGAGCGCCTGCAGGCGATCGCCGCG 
TCGCCGGGCATCGCCAGTGGTCCGGCGCATGTCCAGGTGGCGCAGCGCTTCGAGTTCCAGCCGCGTGGCGAG 
TCGCCGGCCCATGAACGCGAGCGCCTGCTGCGGGCCAAGCGGGCGGTGGACGAGGAGATCGTCGGCCTGGTC 
GAACGCAGCACGGTGAAGGCGATCCGCGAGATTTTCGTCACCCACCGGGAGATGCTCGACGATCCGGAACTG 
GCCGAGCAGGTGCAACTGCGCCTGAATCGCGGCGAAAGCGCCGAAGCGGCCTGGAGTCGGGTAGTGGAAGAC 
AGCGCGGCGCAGCAGGAGGCGCTGCACGACACCTTGCTCGCCGAGCGCGCCGCCGACCTCCGCGACCTCGGC 
CGGCGGGTGCTGGCCAGGCTCTGCGGGGTGGAGGCGCCGCGCGAGCCGGAGCAGCCGTACATCCTGGTGATG 
GACGAGGTCGGCCCCTCCGATGTCGCCCGCCTCGACGCCCAGCGGGTCGCCGGCATCCTCACGGCGCGCGGC 
GGCGCCACTTCGCACAGCGCGATCATCGCCCGCGCCCTCGGCATTCCGGCATTGGTCGGCGCCGGCGCGGCG 
GTGCTCGGCCTGGAGCCGGGCACGGCACTGCTGCTGGATGGCGAACACGGCTGGCTGCAGGTGGCGCCGAGC 
ACCGAACAGTTGCAGCAGGCCGCCGCCGAGCGCGACGCCCGCCAGCAGCGCCAGGCCCGCGCCGACGCGCAG 
CGTCTGGAGCCTGCGCGGACCCGCGACGGCCACGCGGTGGAAGTCTGCGCCAACCTTGGCGACACCGCCGGC 
GCCGCGCGCGCGGTGGAGCTGGGCGCCGAGGGCGTTGGCCTGCTGCGCACCGAGTTCGTCTTCATGAACAAC 
GCCCGCGCGCCGGACCTGGCGACCCAGGAAGCGGAATACCGTCGGGTGCTCGACGCCCTCGACGGACGTCCG 
CTGGTGGCGCGGACCCTGGATGTCGGCGGGGACAAGCCGCTGCCGTACTGGCCGATTTCCCACGAAGAGAAT 
CCCTATCTGGGCCTGCGCGGTATCCGCTTGACCCTGCAGCGACCGCAGATCCTCGAGACCCAGTTGCGCGCG 
CTGTTCCGCGCCGCCGGGGAGCGACCGCTGCGGGTGATGTTTCCGATGGTCGGCAGCCTCGACGAATGGCGC 
CAGGCGCGTGACCTGGCGCTGCGCCTGCGCGAGGAAATCCCGCTGGCCGACCTGCAACTGGGGATCATGGTT 
GAGGTGCCCTCGGCGGCGTTGCTCGCGCCGGTGCTGGCCCGCGAGGTGGATTTCTTCAGCGTCGGCACCAAC 
GACCTGACCCAGTACACCCTGGCCATCGATCGCGGCCACCCGAGTCTTTCGGCCCAGGCCGACGGCCTGCAT 
CCGGCGGTGCTGCAACTGATCGACATGACCGTCCGCGCCGCCCATGCCGAGGGCAAGTGGGTCGGCGTCTGC 
GGCGAACTGGCGGCCGACCCGCTGGCGTTGCCGCTGCTGGTCGGCCTGGGAGTGGACGAGCTGAGCGTCTCG 
GCGCGCAGCATCGCCCTGGTCAAGGCCGGCGTGCGCGAACTGCAGCTGGTCGCTGCGCGCGGCCTGGCCCGC 
AAAGCCCTGGGCCTGGCGAGCGCCGCCGAGGTCCGGGCCCTGGTGGAAGCGGAGGTGCAGTAA