ViewGene
Locus Name:
GeneID:
Location:

GeneID:977
TrivialName: PA14_69130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69130
PAO1 Ortholog Locus:PA5235
Sequence Length:1347
Protein Length:448
Start: 6165123
Stop: 6166469
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08620.1|AE004936_3 (AE004936) glycerol-3-phosphate transporter [Pseudomonas aeruginosa PAO1]
Identities = 448/448 (100%), Positives = 448/448 (100%)
Sequence:
ATGTTCGGAATCTTTCGTCCCGCGCCACACCAGGCGCCACTGCCCGATGCCGAGGTGGACAGCACCTACCGG
CGCCTGCGCTGGCAGATCTTCGCCGGTATCTTCATCGGCTACGCGGGTTACTACCTGCTGCGCAAGAACTTC
TCCCTGGCCATGCCCTACCTCATCGAGGACGGCTACAGTCGCGGCCAGCTGGGCGTCGCGATGTCGGCCATC
GCCATCGCCTACGGCCTGTCGAAATTCATCATGGGCCTGGTCTCGGACCGTTCCAACCCACGGGTCTTCCTG
CCGTTCGGCCTGTTGGTGTCGGCGGCGGTGATGTTCATTTTCGGATTCGCGCACTGGGCTACCTCCAGCGTC
GCCATCATGTTCGTCCTGCTCTTCATTAACGGCTGGGCCCAGGGCATGGGCTGGCCGCCGAGCGGACGGACC
ATGGTCCACTGGTGGTCGCAGAAGGAGCGCGGCAGCGTGGTCTCGGTGTGGAACGTCGCCCACAATGTCGGC
GGCGGCCTGATCGGCCCGCTGTTCCTGCTCGGCCTGGCGCTGTTCGGCGACTGGCGCTCGGCCTTCTACGTG
CCGGCCATCGTCGCCGTGGGCGTAGCCGTGTTCGCCTATTTCGCCATGCGCGACACCCCGCAGTCCTGCGGC
CTGCCGCCGATCGAGAAGCATAAGGACGACTACCCCGAAGGCTACGACGAACACCACGAGCGCGAGCTGTCG
ACCAAGGAGATCTTCGTCACCTACGTGCTGCGCAACAAGCTGCTCTGGTACATCGCCCTGGCCAACGTGTTC
GTCTACCTGCTGCGCTACGGTGTGCTCGACTGGGCGCCGACCTACCTCAAGGAGGCCAAGCACTTCAACGTC
GACAAGTCGTCCTGGGCGTACTTCTTCTACGAATGGGCGGGGATTCCCGGCACCCTGCTCTGCGGCTGGCTG
TCGGACAAGCTGTTCAAGGGCAATCGCGGCGCCACCGGCGTGGTGTTCATGATCCTGGTGACCGTCGGCACC
CTGATCTACTGGCTGAACCCGGCCGGCAACCCGGCGATCGACATGGCCGCGCTGATCATGATCGGCTTCCTG
ATCTACGGCCCGGTGATGCTGATCGGCTTGCAGGCCCTGGAGCTGGCGCCGAAGAAAGCGGCCGGCACCGCC
GCCGGCTTCACCGGTCTGTTCGGCTACCTCGGCGGCTCGGTGGCAGCCAACGCCCTGGTCGGCTACACGGTG
GACCACTTCGGCTGGGACGGTGGCTTCAAGCTGCTGATCGGCGCCTGCCTGCTGGCCATCCTGTTCCTCTGC
CTGACCCTGCGCAAACCCGCGGTCCAGGCCGCGCCGCAGCAAGCCGGCTGA
Translation:
MFGIFRPAPHQAPLPDAEVDSTYRRLRWQIFAGIFIGYAGYYLLRKNFSLAMPYLIEDGYSRGQLGVAMSAI
AIAYGLSKFIMGLVSDRSNPRVFLPFGLLVSAAVMFIFGFAHWATSSVAIMFVLLFINGWAQGMGWPPSGRT
MVHWWSQKERGSVVSVWNVAHNVGGGLIGPLFLLGLALFGDWRSAFYVPAIVAVGVAVFAYFAMRDTPQSCG
LPPIEKHKDDYPEGYDEHHERELSTKEIFVTYVLRNKLLWYIALANVFVYLLRYGVLDWAPTYLKEAKHFNV
DKSSWAYFFYEWAGIPGTLLCGWLSDKLFKGNRGATGVVFMILVTVGTLIYWLNPAGNPAIDMAALIMIGFL
IYGPVMLIGLQALELAPKKAAGTAAGFTGLFGYLGGSVAANALVGYTVDHFGWDGGFKLLIGACLLAILFLC
LTLRKPAVQAAPQQAG*
AnnotationID:986GeneID:977
AnnotatorUID: diggins
Modification Date/Time: 2005-03-16 06:06:18
Gene Name:glpT
Confidence Code:2
GeneProduct:glycerol-3-phosphate transporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:glycerol-3-phosphate permease
Functional Category Confidence Code:5
COGs:COG2271
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58664.1| (AE016875) glycerol-3-phosphate transporter [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 449

 Score =  782 bits (2019), Expect = 0.0
 Identities = 369/440 (83%), Positives = 399/440 (90%)

>gb|AAP17655.1| (AE016986) sn-glycerol-3-phosphate permease [Shigella flexneri 2a
           str. 2457T]
          Length = 452

 Score =  698 bits (1801), Expect = 0.0
 Identities = 320/435 (73%), Positives = 376/435 (86%)

>gb|AAG57371.1|AE005456_8 (AE005456) sn-glycerol-3-phosphate permease [Escherichia coli
           O157:H7 EDL933]
          Length = 452

 Score =  698 bits (1801), Expect = 0.0
 Identities = 320/435 (73%), Positives = 376/435 (86%)








 
Structural Features:
COG2271, UhpC, Sugar phosphate permease [Carbohydrate transport and metabolism]
CD-Length = 448 residues,  97.1% aligned
Score =  560 bits (1444), Expect = 2e-160
Identities = 243/439 (55%), Positives = 309/439 (70%), Gaps = 7/439 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1957




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19099




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34858




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:977_cdd.html


    		




 
URL:

















    ReferenceID:35751




  
Author/Investigator(s):
        Eiglmeier K, Boos W, Cole ST.





  
Title:
        Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system.




  
PubMed:
        3329281


MedLine:
        




    
Source:
Mol Microbiol. 1987 Nov;1(3):251-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3329281

















    ReferenceID:35752




  
Author/Investigator(s):
        Nilsson RP, Beijer L, Rutberg B.





  
Title:
        The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis.




  
PubMed:
        8012593


MedLine:
        




    
Source:
Microbiology. 1994 Apr;140 ( Pt 4):723-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8012593

















    ReferenceID:35753




  
Author/Investigator(s):
        Haga K, Liu H, Takahashi H, Yoshikawa H.





  
Title:
        Sequence analysis of a 45-kb segment in the 19 degrees-23 degrees region of the Bacillus subtilis chromosome containing glpT and mpr loci.




  
PubMed:
        9455481


MedLine:
        




    
Source:
DNA Res. 1997 Oct 31;4(5):329-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9455481









           
Homologs By Global Alignment 

           

       



     
Gene ID:977
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1746
                           PA5235_tr
                           translation of PA5235
                           448
                           99.99
                           99.99
                           0
                           2385.0
     

     
     
61570
                           gb|AAG08620.1|AE004936_3
                           (AE004936) glycerol-3-phosphate transporter [Pseudomonas aeruginosa PAO1]
                           448
                           99.99
                           99.99
                           0
                           2385.0
     

     
     
61571
                           gb|AAO58664.1|
                           (AE016875) glycerol-3-phosphate transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           449
                           82.18
                           89.30
                           1
                           2016.0
     

     
     
61572
                           gb|AAQ57932.1|
                           (AE016910) glycerol-3-phosphate permease [Chromobacterium violaceum ATCC 12472]
                           448
                           76.11
                           85.26
                           1
                           1892.0
     

     
     
61575
                           dbj|BAB36548.1|
                           (AP002561) sn-glycerol-3-phosphate permease [Escherichia coli O157:H7]
                           452
                           71.46
                           84.07
                           4
                           1794.0
     

     






      

          
GC ORFID: 43979How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5235Glimmer Score: 

          
Start: 5428810Stop: 5430156

          
Length: 1347


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5235 translation of PA5235Homolog Bit Score 923.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3866 (ORF: BLASTX 780.0), t_PA5235 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCGGAATCTTTCGTCCCGCGCCACACCAGGCGCCACTGCCCGATGCCGAGGTGGACAGCACCTACCGG 
CGCCTGCGCTGGCAGATCTTCGCCGGTATCTTCATCGGCTACGCGGGTTACTACCTGCTGCGCAAGAACTTC 
TCCCTGGCCATGCCCTACCTCATCGAGGACGGCTACAGTCGCGGCCAGCTGGGCGTCGCGATGTCGGCCATC 
GCCATCGCCTACGGCCTGTCGAAATTCATCATGGGCCTGGTCTCGGACCGTTCCAACCCACGGGTCTTCCTG 
CCGTTCGGCCTGTTGGTGTCGGCGGCGGTGATGTTCATTTTCGGATTCGCGCACTGGGCTACCTCCAGCGTC 
GCCATCATGTTCGTCCTGCTCTTCATTAACGGCTGGGCCCAGGGCATGGGCTGGCCGCCGAGCGGACGGACC 
ATGGTCCACTGGTGGTCGCAGAAGGAGCGCGGCAGCGTGGTCTCGGTGTGGAACGTCGCCCACAATGTCGGC 
GGCGGCCTGATCGGCCCGCTGTTCCTGCTCGGCCTGGCGCTGTTCGGCGACTGGCGCTCGGCCTTCTACGTG 
CCGGCCATCGTCGCCGTGGGCGTAGCCGTGTTCGCCTATTTCGCCATGCGCGACACCCCGCAGTCCTGCGGC 
CTGCCGCCGATCGAGAAGCATAAGGACGACTACCCCGAAGGCTACGACGAACACCACGAGCGCGAGCTGTCG 
ACCAAGGAGATCTTCGTCACCTACGTGCTGCGCAACAAGCTGCTCTGGTACATCGCCCTGGCCAACGTGTTC 
GTCTACCTGCTGCGCTACGGTGTGCTCGACTGGGCGCCGACCTACCTCAAGGAGGCCAAGCACTTCAACGTC 
GACAAGTCGTCCTGGGCGTACTTCTTCTACGAATGGGCGGGGATTCCCGGCACCCTGCTCTGCGGCTGGCTG 
TCGGACAAGCTGTTCAAGGGCAATCGCGGCGCCACCGGCGTGGTGTTCATGATCCTGGTGACCGTCGGCACC 
CTGATCTACTGGCTGAACCCGGCCGGCAACCCGGCGATCGACATGGCCGCGCTGATCATGATCGGCTTCCTG 
ATCTACGGCCCGGTGATGCTGATCGGCTTGCAGGCCCTGGAGCTGGCGCCGAAGAAAGCGGCCGGCACCGCC 
GCCGGCTTCACCGGTCTGTTCGGCTACCTCGGCGGCTCGGTGGCAGCCAACGCCCTGGTCGGCTACACGGTG 
GACCACTTCGGCTGGGACGGTGGCTTCAAGCTGCTGATCGGCGCCTGCCTGCTGGCCATCCTGTTCCTCTGC 
CTGACCCTGCGCAAACCCGCGGTCCAGGCCGCGCCGCAGCAAGCCGGCTGA