ViewGene
Locus Name:
GeneID:
Location:

GeneID:976
TrivialName: PA14_62560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62560
PAO1 Ortholog Locus:PA4727
Sequence Length:1404
Protein Length:467
Start: 5581256
Stop: 5582659
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08113.1|AE004886_5 (AE004886) poly(A) polymerase [Pseudomonas aeruginosa PAO1]
Identities = 466/467 (99%), Positives = 466/467 (99%)
Sequence:
ATGCTGAAAAAGCTGATCCAGACCATTCGTTCCCCCCTGCGCCGTCCGCGCGCCGTCCGTACAACCCCGGAA
GTCATCGGTAACAACCAGCACTCCCTGCGCCGGGACCAGTTCAGCCGGAACGCCGTCAAGGTCGTCGAGATC
CTCCAGCGGGCCGGCTACCAGGCCTACGTGGTCGGGGGCTGCGTACGCGACCAGATGCTCGGCATCGCTCCC
AAGGACTTCGATGTCGCCACCAGCGCCACGCCGGAGCAGGTCCGCGCCGAATTCCGCAACGCGCGGATCATC
GGCCGCCGCTTCAAACTGGTACACGTGCACTTCGGCCGCGAGATCATCGAGGTCGCCACCTTCCGCGCCCAT
CATCCGGAAGGCCAGGACGATGGCGATAGCCGCGCGTCCAGCAACGAAAGCGGCCGCATCCTGCGCGACAAC
GTCTATGGCAGCCTGGAAGACGACGCCCAGCGCCGCGACTTCACCATCAATGCCCTGTACTTCGACGTCACC
AGCGAGCGGCTGCTGGACTACGCCAATGGCGTTCACGACATCCGCAATCGCCTGATCCGCCTGATCGGCGAT
CCGGAGCAGCGTTATCTGGAAGACCCGGTGCGGATGCTGCGGGCGGTACGTTTCGCCGCCAAGCTCGACTTC
GAGATCGAAAAGCACAGCGCCACGCCGATTCGCCGGCTGGCCCCGCTGCTGCGGGAAATCCCTTCGGCGCGC
CTGTTCGACGAGGTGCTCAAGCTGTTCCTCGCCGGCCGGGCCGAGCGCACTTTCGAACTGCTGGTCGAGTAC
GAACTGTTCGCGCCGCTGTTCCCGGCCAGCGCCAAGGCACTGCAGGCCAACCCCGACTACACCGGCAAGCTG
ATCCGCCAGGCCCTGGCCAATACCGATGCGCGAATTCGCCAGGGCAAGCCGGTAACCCCGGCCTTCCTCTTC
GCCGCACTGCTCTGGCCGGCCCTGCCGGCACGCGTCGCGCAGTTGCAGGAGAAAGGCATGCCGGCCATCCCG
GCGATGCAGGAAGCCGCCCACGAACTGATCAGCGAACAGTGCCAGCGGATCGCCATTCCCAAGCGTTTCACC
CTGCCGATCCGCGAGATCTGGGATATGCAGGAACGTCTGCCGCGACGCCAGGGCAAGCGCGCCGACCTGCTC
CTGGAAAACCCGCGCTTCCGCGCCGGCTACGACTTCCTCCTGCTGCGCGAAAGTGCCGGCGAGGAAACCGAA
GGACTCGGCCAATGGTGGACCGATTACCAGGAAGTCAGCGACAGCGAGCGGCGCAACATGATCCGCGACCTG
GTCAGCCAGGAAGACGGCAGCGCCCCGCGCAAGCGTCGCCGCGGCGGCAACAGCGGCCGTCGGCGCCGCGGG
CCGCGCAAGGAAGGCAGCGGCGGAAGCGGCGAATGA
Translation:
MLKKLIQTIRSPLRRPRAVRTTPEVIGNNQHSLRRDQFSRNAVKVVEILQRAGYQAYVVGGCVRDQMLGIAP
KDFDVATSATPEQVRAEFRNARIIGRRFKLVHVHFGREIIEVATFRAHHPEGQDDGDSRASSNESGRILRDN
VYGSLEDDAQRRDFTINALYFDVTSERLLDYANGVHDIRNRLIRLIGDPEQRYLEDPVRMLRAVRFAAKLDF
EIEKHSATPIRRLAPLLREIPSARLFDEVLKLFLAGRAERTFELLVEYELFAPLFPASAKALQANPDYTGKL
IRQALANTDARIRQGKPVTPAFLFAALLWPALPARVAQLQEKGMPAIPAMQEAAHELISEQCQRIAIPKRFT
LPIREIWDMQERLPRRQGKRADLLLENPRFRAGYDFLLLRESAGEETEGLGQWWTDYQEVSDSERRNMIRDL
VSQEDGSAPRKRRRGGNSGRRRRGPRKEGSGGSGE*
AnnotationID:985GeneID:976
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 06:06:48
Gene Name:pcnB
Confidence Code:2
GeneProduct:poly(A) polymerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:polynucleotide adenyltransferase
Functional Category Confidence Code:5
COGs:COG0617,pfam01743
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:2.7.7.19
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO60158.1| (AY210414) polynucleotide adenylyltransferase; PcnB [Pseudomonas
           fluorescens]
          Length = 465

 Score =  721 bits (1860), Expect = 0.0
 Identities = 357/458 (77%), Positives = 406/458 (88%), Gaps = 2/458 (0%)

>gb|AAO54497.1| (AE016859) poly(A) polymerase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 467

 Score =  715 bits (1845), Expect = 0.0
 Identities = 366/471 (77%), Positives = 409/471 (86%), Gaps = 8/471 (1%)

>gb|AAM84375.1|AE013681_5 (AE013681) poly(A) polymerase I [Yersinia pestis KIM]
          Length = 491

 Score =  419 bits (1078), Expect = e-116
 Identities = 225/443 (50%), Positives = 297/443 (67%), Gaps = 12/443 (2%)






 
Structural Features:
COG0617, PcnB, tRNA nucleotidyltransferase/poly(A) polymerase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 412 residues,  95.6% aligned
Score =  306 bits (784), Expect = 4e-84
Identities = 153/406 (37%), Positives = 212/406 (52%), Gaps = 19/406 (4%)

pfam01743, PolyA_pol, Poly A polymerase family. 
CD-Length = 158 residues,  98.1% aligned
Score =  151 bits (384), Expect = 1e-37
Identities = 70/165 (42%), Positives = 94/165 (56%), Gaps = 11/165 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1955




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19097




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37640




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:976_cdd.html


    		




 
URL:

















    ReferenceID:37741




  
Author/Investigator(s):
        Liu JD, Parkinson JS.





  
Title:
        Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control.




  
PubMed:
        2537812


MedLine:
        




    
Source:
J Bacteriol. 1989 Mar;171(3):1254-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2537812









           
Homologs By Global Alignment 

           

       



     
Gene ID:976
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1745
                           PA4727_tr
                           translation of PA4727
                           467
                           99.78
                           99.78
                           0
                           2385.0
     

     
     
61528
                           gb|AAG08113.1|AE004886_5
                           (AE004886) poly(A) polymerase [Pseudomonas aeruginosa PAO1]
                           467
                           99.78
                           99.78
                           0
                           2385.0
     

     
     
61530
                           gb|AAO54497.1|
                           (AE016859) poly(A) polymerase [Pseudomonas syringae pv. tomato str. DC3000]
                           471
                           77.70
                           86.83
                           8
                           1856.0
     

     
     
61529
                           gb|AAO60158.1|
                           (AY210414) polynucleotide adenylyltransferase; PcnB [Pseudomonas fluorescens]
                           470
                           76.38
                           86.80
                           8
                           1865.0
     

     
     
61531
                           gb|AAN70270.1|AE016667_3
                           (AE016791) poly(A) polymerase [Pseudomonas putida KT2440]
                           472
                           73.72
                           82.62
                           17
                           1779.0
     

     






      

          
GC ORFID: 43978How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4727Glimmer Score: 

          
Start: 4844936Stop: 4846339

          
Length: 1404


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4727 translation of PA4727Homolog Bit Score 923.0

          
Other Homologs:  t_PA4727 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGAAAAAGCTGATCCAGACCATTCGTTCCCCCCTGCGCCGTCCGCGCGCCGTCCGTACAACCCCGGAA 
GTCATCGGTAACAACCAGCACTCCCTGCGCCGGGACCAGTTCAGCCGGAACGCCGTCAAGGTCGTCGAGATC 
CTCCAGCGGGCCGGCTACCAGGCCTACGTGGTCGGGGGCTGCGTACGCGACCAGATGCTCGGCATCGCTCCC 
AAGGACTTCGATGTCGCCACCAGCGCCACGCCGGAGCAGGTCCGCGCCGAATTCCGCAACGCGCGGATCATC 
GGCCGCCGCTTCAAACTGGTACACGTGCACTTCGGCCGCGAGATCATCGAGGTCGCCACCTTCCGCGCCCAT 
CATCCGGAAGGCCAGGACGATGGCGATAGCCGCGCGTCCAGCAACGAAAGCGGCCGCATCCTGCGCGACAAC 
GTCTATGGCAGCCTGGAAGACGACGCCCAGCGCCGCGACTTCACCATCAATGCCCTGTACTTCGACGTCACC 
AGCGAGCGGCTGCTGGACTACGCCAATGGCGTTCACGACATCCGCAATCGCCTGATCCGCCTGATCGGCGAT 
CCGGAGCAGCGTTATCTGGAAGACCCGGTGCGGATGCTGCGGGCGGTACGTTTCGCCGCCAAGCTCGACTTC 
GAGATCGAAAAGCACAGCGCCACGCCGATTCGCCGGCTGGCCCCGCTGCTGCGGGAAATCCCTTCGGCGCGC 
CTGTTCGACGAGGTGCTCAAGCTGTTCCTCGCCGGCCGGGCCGAGCGCACTTTCGAACTGCTGGTCGAGTAC 
GAACTGTTCGCGCCGCTGTTCCCGGCCAGCGCCAAGGCACTGCAGGCCAACCCCGACTACACCGGCAAGCTG 
ATCCGCCAGGCCCTGGCCAATACCGATGCGCGAATTCGCCAGGGCAAGCCGGTAACCCCGGCCTTCCTCTTC 
GCCGCACTGCTCTGGCCGGCCCTGCCGGCACGCGTCGCGCAGTTGCAGGAGAAAGGCATGCCGGCCATCCCG 
GCGATGCAGGAAGCCGCCCACGAACTGATCAGCGAACAGTGCCAGCGGATCGCCATTCCCAAGCGTTTCACC 
CTGCCGATCCGCGAGATCTGGGATATGCAGGAACGTCTGCCGCGACGCCAGGGCAAGCGCGCCGACCTGCTC 
CTGGAAAACCCGCGCTTCCGCGCCGGCTACGACTTCCTCCTGCTGCGCGAAAGTGCCGGCGAGGAAACCGAA 
GGACTCGGCCAATGGTGGACCGATTACCAGGAAGTCAGCGACAGCGAGCGGCGCAACATGATCCGCGACCTG 
GTCAGCCAGGAAGACGGCAGCGCCCCGCGCAAGCGTCGCCGCGGCGGCAACAGCGGCCGTCGGCGCCGCGGG 
CCGCGCAAGGAAGGCAGCGGCGGAAGCGGCGAATGA