ViewGene
Locus Name:
GeneID:
Location:

GeneID:968
TrivialName: PA14_62540
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62540
PAO1 Ortholog Locus:PA4726
Sequence Length:1437
Protein Length:478
Start: 5578920
Stop: 5580356
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08112.1|AE004886_4 (AE004886) two-component response regulator CbrB [Pseudomonas aeruginosa PAO1]
Identities = 477/478 (99%), Positives = 477/478 (99%)
Sequence:
ATGGCACATATTCTGATCGTCGAAGACGAAACCATTATCCGATCTGCATTGCGACGCCTGCTGGAGCGCAAC
CAGTACCAGGTCAGCGAAGCCGGCTCGGTGCAGGAAGCCCAGGAGCGCTACACCATCCCCTCCTTCGACATG
GTGGTCAGCGACCTGCGCCTGCCGGGCGCCCCCGGCACCGAACTGATCAAGCTCGCCGAAGGCATTCCGGTG
CTGATCATGACCAGCTACGCCAGCCTGCGTTCGGCGGTGGATTCGATGAAGATGGGCGCGGTGGACTACATC
GCCAAGCCTTTCGACCACGACGAAATGCTCCAGGCCGTGGCGCGCATCCTCAAGGACCGCCAGGAGAACCGC
AGCGCGGCCCCGGTCTCCGCCAACGGCGGCAAGGCCGGCGGCGAACGCGGCGCCAGCCCGGCGGCAGCCGAC
GGCGAGATAGGCATCATCGGCTCCTGTGCGCCGATGCAAGAGCTGTACAGCAAGATTCGCAAGGTCGCGCCG
ACCGACTCCACGGTACTGATCCAGGGCGAGTCCGGCACCGGCAAGGAACTGGTCGCCCGCGCCCTGCACAAC
CTGTCGAAGCGCGCCAAGGCGCCGCTGATCTCGGTGAACTGTGCGGCGATTCCGGAAACCCTGATCGAATCC
GAGCTGTTCGGCCATGAAAAAGGCGCCTTCACCGGTGCCAGCGCCGGGCGCGCCGGGCTGGTCGAAGCAGCT
GACGGCGGCACCCTGTTCCTCGACGAGATCGGCGAACTGCCGCTGGAGGCCCAGGCCCGCCTGCTGCGCGTA
TTGCAGGAAGGCGAGATCCGCCGGGTCGGCTCGGTGCAATCGCAGAAGGTCGACGTGCGCCTGATCGCCGCC
ACCCACCGCGACCTGAAGACCCTGGCCAAGACCGGGCAATTCCGCGAAGACCTCTATTACCGCCTGCATGTG
ATCTCGCTGAAGCTGCCCGCCCTGCGCGAGCGTGGCAACGACGTGATGGAGATCGCCCGCGCCTTTCTGGCT
CGCCAGTGCACCCGGATGGGGCGCGCGCCGCTCAGCTTCGCCCACGACGCCGAACAGGCGATCCGCCATTAC
CCATGGCCGGGCAACGTGCGCGAGCTGGAAAACGCCATCGAGCGGGCGGTGATCCTCAGCGAGAGCCAGGAA
ATCCACGCCGACCTGCTGGGTATCGACATTGAGCTGGACGACCTCGAGGACGACTTCGCCGCCGACCTCGGC
CTCGGCCCGGCGGGCGCCGCGGCCAGCAACCACGAGCCCACCGAGGACCTTTCGCTGGAAGACTACTTCCAG
CACTTCGTCCTCGAGCACCAGGACCACATGACCGAGACCGAGCTGGCCCGCAAGCTGGGCATCAGCCGCAAA
TGCCTGTGGGAACGCCGCCAGCGCCTGGGCATTCCGCGACGCAAATCAGGGGCCTCGGCCGACTCGTAA
Translation:
MAHILIVEDETIIRSALRRLLERNQYQVSEAGSVQEAQERYTIPSFDMVVSDLRLPGAPGTELIKLAEGIPV
LIMTSYASLRSAVDSMKMGAVDYIAKPFDHDEMLQAVARILKDRQENRSAAPVSANGGKAGGERGASPAAAD
GEIGIIGSCAPMQELYSKIRKVAPTDSTVLIQGESGTGKELVARALHNLSKRAKAPLISVNCAAIPETLIES
ELFGHEKGAFTGASAGRAGLVEAADGGTLFLDEIGELPLEAQARLLRVLQEGEIRRVGSVQSQKVDVRLIAA
THRDLKTLAKTGQFREDLYYRLHVISLKLPALRERGNDVMEIARAFLARQCTRMGRAPLSFAHDAEQAIRHY
PWPGNVRELENAIERAVILSESQEIHADLLGIDIELDDLEDDFAADLGLGPAGAAASNHEPTEDLSLEDYFQ
HFVLEHQDHMTETELARKLGISRKCLWERRQRLGIPRRKSGASADS*
AnnotationID:977GeneID:968
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 07:07:42
Gene Name:cbrB
Confidence Code:1
GeneProduct:two-component response regulator CbrB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3829,pfam00158,COG1221
Secondary Functional Category(ies):signal transduction mechanisms
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70269.1|AE016667_2 (AE016791) nitrogen regulation protein NR(I) [Pseudomonas putida
           KT2440]
          Length = 480

 Score =  774 bits (1998), Expect = 0.0
 Identities = 402/480 (83%), Positives = 432/480 (90%), Gaps = 3/480 (0%)

>gb|AAO54498.1| (AE016859) sigma-54 dependent transcriptional regulator/response
           regulator [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 478

 Score =  745 bits (1924), Expect = 0.0
 Identities = 387/476 (81%), Positives = 422/476 (88%), Gaps = 1/476 (0%)








 
Structural Features:
COG2204, AtoC, Response regulator containing CheY-like receiver, 
AAA-type ATPase, and DNA-binding domains 
[Signal transduction mechanisms]
CD-Length = 464 residues,  99.4% aligned
Score =  480 bits (1238), Expect = 1e-136
Identities = 218/479 (45%), Positives = 284/479 (59%), Gaps = 22/479 (4%)

pfam00158, Sigma54_activat, Sigma-54 interaction domain.
CD-Length = 222 residues, 100.0% aligned
Score =  318 bits (817), Expect = 8e-88
Identities = 135/222 (60%), Positives = 165/222 (74%)

COG1221, PspF, Transcriptional regulators containing an 
AAA-type ATPase domain and a DNA-binding domain 
[Transcription / Signal transduction mechanisms]
CD-Length = 403 residues,  79.4% aligned
Score =  276 bits (708), Expect = 3e-75
Identities = 145/324 (44%), Positives = 191/324 (58%), Gaps = 8/324 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1939




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19081




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37651




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:968_cdd.html


    		




 
URL:

















    ReferenceID:37743




  
Author/Investigator(s):
        Nishijyo T, Haas D, Itoh Y.





  
Title:
        The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa.




  
PubMed:
        11401699


MedLine:
        




    
Source:
Mol Microbiol. 2001 May;40(4):917-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11401699









           
Homologs By Global Alignment 

           

       



     
Gene ID:968
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1715
                           PA4726_tr
                           translation of PA4726
                           478
                           99.79
                           99.79
                           0
                           2394.0
     

     
     
60777
                           dbj|BAB20867.1|
                           (AB045630) CbrB [Pseudomonas aeruginosa]
                           478
                           99.79
                           99.79
                           0
                           2394.0
     

     
     
60776
                           gb|AAG08112.1|AE004886_4
                           (AE004886) two-component response regulator CbrB [Pseudomonas aeruginosa PAO1]
                           478
                           99.79
                           99.79
                           0
                           2394.0
     

     
     
60778
                           gb|AAN70269.1|AE016667_2
                           (AE016791) nitrogen regulation protein NR(I) [Pseudomonas putida KT2440]
                           481
                           83.57
                           89.81
                           4
                           2001.0
     

     
     
60779
                           gb|AAO54498.1|
                           (AE016859) sigma-54 dependent transcriptional regulator/response regulator [Pseudomonas syringae pv. tomato str. DC3000]
                           486
                           79.83
                           87.24
                           16
                           1927.0
     

     






      

          
GC ORFID: 43970How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4726Glimmer Score: 

          
Start: 4842600Stop: 4844036

          
Length: 1437


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4726 translation of PA4726Homolog Bit Score 926.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2516 (ORF: BLASTX 760.0), psyr_15may02_Scaffold8_revised_gene7916 (ORF: BLASTX 314.0), psyr_15may02_Scaffold6_revised_gene6894 (ORF: BLASTX 304.0), t_PA1099 (ORF: BLASTX 286.0), t_PA2359 (ORF: BLASTX 250.0), psyr_15may02_Scaffold1_revised_gene1290 (ORF: BLASTX 246.0), t_PA1097 (ORF: BLASTX 245.0), psyr_15may02_Scaffold2_revised_gene4053 (ORF: BLASTX 235.0), t_PA1196 (ORF: BLASTX 206.0), t_PA4726 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACATATTCTGATCGTCGAAGACGAAACCATTATCCGATCTGCATTGCGACGCCTGCTGGAGCGCAAC 
CAGTACCAGGTCAGCGAAGCCGGCTCGGTGCAGGAAGCCCAGGAGCGCTACACCATCCCCTCCTTCGACATG 
GTGGTCAGCGACCTGCGCCTGCCGGGCGCCCCCGGCACCGAACTGATCAAGCTCGCCGAAGGCATTCCGGTG 
CTGATCATGACCAGCTACGCCAGCCTGCGTTCGGCGGTGGATTCGATGAAGATGGGCGCGGTGGACTACATC 
GCCAAGCCTTTCGACCACGACGAAATGCTCCAGGCCGTGGCGCGCATCCTCAAGGACCGCCAGGAGAACCGC 
AGCGCGGCCCCGGTCTCCGCCAACGGCGGCAAGGCCGGCGGCGAACGCGGCGCCAGCCCGGCGGCAGCCGAC 
GGCGAGATAGGCATCATCGGCTCCTGTGCGCCGATGCAAGAGCTGTACAGCAAGATTCGCAAGGTCGCGCCG 
ACCGACTCCACGGTACTGATCCAGGGCGAGTCCGGCACCGGCAAGGAACTGGTCGCCCGCGCCCTGCACAAC 
CTGTCGAAGCGCGCCAAGGCGCCGCTGATCTCGGTGAACTGTGCGGCGATTCCGGAAACCCTGATCGAATCC 
GAGCTGTTCGGCCATGAAAAAGGCGCCTTCACCGGTGCCAGCGCCGGGCGCGCCGGGCTGGTCGAAGCAGCT 
GACGGCGGCACCCTGTTCCTCGACGAGATCGGCGAACTGCCGCTGGAGGCCCAGGCCCGCCTGCTGCGCGTA 
TTGCAGGAAGGCGAGATCCGCCGGGTCGGCTCGGTGCAATCGCAGAAGGTCGACGTGCGCCTGATCGCCGCC 
ACCCACCGCGACCTGAAGACCCTGGCCAAGACCGGGCAATTCCGCGAAGACCTCTATTACCGCCTGCATGTG 
ATCTCGCTGAAGCTGCCCGCCCTGCGCGAGCGTGGCAACGACGTGATGGAGATCGCCCGCGCCTTTCTGGCT 
CGCCAGTGCACCCGGATGGGGCGCGCGCCGCTCAGCTTCGCCCACGACGCCGAACAGGCGATCCGCCATTAC 
CCATGGCCGGGCAACGTGCGCGAGCTGGAAAACGCCATCGAGCGGGCGGTGATCCTCAGCGAGAGCCAGGAA 
ATCCACGCCGACCTGCTGGGTATCGACATTGAGCTGGACGACCTCGAGGACGACTTCGCCGCCGACCTCGGC 
CTCGGCCCGGCGGGCGCCGCGGCCAGCAACCACGAGCCCACCGAGGACCTTTCGCTGGAAGACTACTTCCAG 
CACTTCGTCCTCGAGCACCAGGACCACATGACCGAGACCGAGCTGGCCCGCAAGCTGGGCATCAGCCGCAAA 
TGCCTGTGGGAACGCCGCCAGCGCCTGGGCATTCCGCGACGCAAATCAGGGGCCTCGGCCGACTCGTAA