ViewGene
Locus Name:
GeneID:
Location:

GeneID:967
TrivialName: PA14_25390
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25390
PAO1 Ortholog Locus:PA2991
Sequence Length:1395
Protein Length:464
Start: 2219113
Stop: 2220507
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06379.1|AE004724_8 (AE004724) soluble pyridine nucleotide transhydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 464/464 (100%), Positives = 464/464 (100%)
Sequence:
ATGGCTGTCTACAACTACGACGTGGTGATTTTGGGCACGGGTCCGGCGGGCGAAGGGGCGGCGATGAACGCC
TCCAAGTACGGACGCAAGCTGGCGGTAGTCGACAGTCGTCGCGTGGTCGGAGGCAACTGCACGCACCTCGGC
ACCATTCCTTCCAAGGCCCTGCGCCACTCGGTGAAGCAGATCATCGAGTTCAACACCAACCCGATGTTCCGC
CAGATCGGCGAGCCGCGCTGGTTCTCCTTCCCCGACGTGCTGAAGAGCGCCGACCGGGTGATCTCCAAGCAG
GTCGCCTCGCGTACCGGCTACTACGCGCGCAATCGCATCGACATGTTCACTGGCACCGCCAGCTTCGTCGAC
GAACGCACCGTCGAAGTGGTGACGCCGAGCGGCGCGGTGGAGCGCCTGGTCGCCGACCAGTTCGTGATCGCC
ACCGGCTCGCGCCCGTATCGTCCTTCGGACATCAATTTCAACCACCCGCGGGTCTACGACAGCGACACCATC
CTGTCCCTGAGCCACACCCCGCGCCGGCTGATCATCTACGGAGCCGGGGTGATCGGCTGCGAATACGCGTCG
ATCTTCAGCGGCCTGGGCGTGCTGGTGGACCTGATCGATACGCGCGACCAGTTGCTCAGCTTCCTCGATGAC
GAGATTTCCGATGCGCTGAGCTACCACCTGCGCAACAACAACGTGCTGATCCGCCACAACGAGGAGTACGAG
CGCGTCGAGGGGCTGGATAACGGCGTCATCCTGCACCTGAAGTCGGGCAAGAAGATCAAGGCGGACGCGCTG
CTCTGGTGCAACGGCCGGACCGGCAATACCGACAAGCTCGGCCTGGAGAACGTCGGCATCAAGGTCAACAGC
CGTGGCCAGATCGAGGTGGACGAGAACTACCGCACTTCGGTGAGCAACATCTTCGCCGCCGGCGACGTGATC
GGCTGGCCGAGCCTGGCCAGCGCTGCCTACGACCAGGGCCGCTCGGCCGCCGGCAACATCGTCGAGAGCGAC
AGCTGGCGCTTCGTCAACGATGTGCCGACCGGCATCTACACCATTCCGGAGATCAGCTCGATCGGCAAGAAC
GAAAGCGAACTGACCGCGGCGAAGATTCCCTACGAAGTGGGCAAGGCGTTCTTCAAGGGCATGGCCCGGGCG
CAGATTTCCAACGAGCCGGTGGGCATGCTGAAGATCCTGTTCCATCGCGAGACTCTGGAGATCCTCGGCGTG
CACTGCTTCGGCGACCAGGCTTCGGAAATCGTCCACATCGGCCAGGCGATCATGAACCAGCCGGGCGAGCTG
AATACCCTGAAGTACTTCGTCAACACCACCTTCAACTACCCGACCATGGCGGAAGCCTATCGGGTAGCGGCG
TTCGACGGCCTCAACCGGCTTTTTTGA
Translation:
MAVYNYDVVILGTGPAGEGAAMNASKYGRKLAVVDSRRVVGGNCTHLGTIPSKALRHSVKQIIEFNTNPMFR
QIGEPRWFSFPDVLKSADRVISKQVASRTGYYARNRIDMFTGTASFVDERTVEVVTPSGAVERLVADQFVIA
TGSRPYRPSDINFNHPRVYDSDTILSLSHTPRRLIIYGAGVIGCEYASIFSGLGVLVDLIDTRDQLLSFLDD
EISDALSYHLRNNNVLIRHNEEYERVEGLDNGVILHLKSGKKIKADALLWCNGRTGNTDKLGLENVGIKVNS
RGQIEVDENYRTSVSNIFAAGDVIGWPSLASAAYDQGRSAAGNIVESDSWRFVNDVPTGIYTIPEISSIGKN
ESELTAAKIPYEVGKAFFKGMARAQISNEPVGMLKILFHRETLEILGVHCFGDQASEIVHIGQAIMNQPGEL
NTLKYFVNTTFNYPTMAEAYRVAAFDGLNRLF*
AnnotationID:976GeneID:967
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 13:01:04
Gene Name:sth
Confidence Code:2
GeneProduct:soluble pyridine nucleotide transhydrogenase
Cell Localization:()
Synonyms:sthA,udhA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1249,pfam00070
Secondary Functional Category(ies):
EC Number:1.6.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAB50562.1| (U91523) soluble pyridine nucleotide transhydrogenase [Pseudomonas
           fluorescens]
          Length = 464

 Score =  820 bits (2118), Expect = 0.0
 Identities = 395/464 (85%), Positives = 435/464 (93%)

>gb|AAD40691.1|AF159108_1 (AF159108) soluble pyridine nucleotide transhydrogenase
           [Azotobacter vinelandii]
          Length = 464

 Score =  813 bits (2099), Expect = 0.0
 Identities = 398/464 (85%), Positives = 430/464 (92%)

>gb|AAN83351.1|AE016770_151 (AE016770) Soluble pyridine nucleotide transhydrogenase
           [Escherichia coli CFT073]
          Length = 466

 Score =  602 bits (1552), Expect = e-171
 Identities = 288/462 (62%), Positives = 368/462 (79%), Gaps = 1/462 (0%)






 
Structural Features:
COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase 
(E3) component, and related enzymes 
[Energy production and conversion]

pfam00070, Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase. 








   
Genomic Context:






   
Comment:


















    ReferenceID:1937




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19079




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36063




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:967_cdd.html


    		




 
URL:

















    ReferenceID:36452




  
Author/Investigator(s):
        French CE, Boonstra B, Bufton KA, Bruce NC.





  
Title:
        Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens.




  
PubMed:
        9098078


MedLine:
        




    
Source:
J Bacteriol. 1997 Apr;179(8):2761-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9098078

















    ReferenceID:36453




  
Author/Investigator(s):
        Boonstra B, Bjorklund L, French CE, Wainwright I, Bruce NC.





  
Title:
        Cloning of the sth gene from Azotobacter vinelandii and construction of chimeric soluble pyridine nucleotide transhydrogenases.




  
PubMed:
        11004404


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2000 Oct 1;191(1):87-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11004404









           
Homologs By Global Alignment 

           

       



     
Gene ID:967
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1714
                           PA2991_tr
                           translation of PA2991
                           464
                           99.99
                           99.99
                           0
                           2394.0
     

     
     
60746
                           gb|AAG06379.1|AE004724_8
                           (AE004724) soluble pyridine nucleotide transhydrogenase [Pseudomonas aeruginosa PAO1]
                           464
                           99.99
                           99.99
                           0
                           2394.0
     

     
     
60750
                           gb|AAD40691.1|AF159108_1
                           (AF159108) soluble pyridine nucleotide transhydrogenase [Azotobacter vinelandii]
                           464
                           85.77
                           92.67
                           0
                           2099.0
     

     
     
60749
                           gb|AAN67764.1|AE016408_2
                           (AE016782) soluble pyridine nucleotide transhydrogenase [Pseudomonas putida KT2440]
                           464
                           85.34
                           93.10
                           0
                           2108.0
     

     
     
60747
                           gb|AAB50562.1|
                           (U91523) soluble pyridine nucleotide transhydrogenase [Pseudomonas fluorescens]
                           464
                           85.12
                           93.75
                           0
                           2118.0
     

     






      

          
GC ORFID: 43969How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2991Glimmer Score: 

          
Start: 2734812Stop: 2733418

          
Length: 1395


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2991 translation of PA2991Homolog Bit Score 926.0

          
Other Homologs:  t_PA2991 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGTCTACAACTACGACGTGGTGATTTTGGGCACGGGTCCGGCGGGCGAAGGGGCGGCGATGAACGCC 
TCCAAGTACGGACGCAAGCTGGCGGTAGTCGACAGTCGTCGCGTGGTCGGAGGCAACTGCACGCACCTCGGC 
ACCATTCCTTCCAAGGCCCTGCGCCACTCGGTGAAGCAGATCATCGAGTTCAACACCAACCCGATGTTCCGC 
CAGATCGGCGAGCCGCGCTGGTTCTCCTTCCCCGACGTGCTGAAGAGCGCCGACCGGGTGATCTCCAAGCAG 
GTCGCCTCGCGTACCGGCTACTACGCGCGCAATCGCATCGACATGTTCACTGGCACCGCCAGCTTCGTCGAC 
GAACGCACCGTCGAAGTGGTGACGCCGAGCGGCGCGGTGGAGCGCCTGGTCGCCGACCAGTTCGTGATCGCC 
ACCGGCTCGCGCCCGTATCGTCCTTCGGACATCAATTTCAACCACCCGCGGGTCTACGACAGCGACACCATC 
CTGTCCCTGAGCCACACCCCGCGCCGGCTGATCATCTACGGAGCCGGGGTGATCGGCTGCGAATACGCGTCG 
ATCTTCAGCGGCCTGGGCGTGCTGGTGGACCTGATCGATACGCGCGACCAGTTGCTCAGCTTCCTCGATGAC 
GAGATTTCCGATGCGCTGAGCTACCACCTGCGCAACAACAACGTGCTGATCCGCCACAACGAGGAGTACGAG 
CGCGTCGAGGGGCTGGATAACGGCGTCATCCTGCACCTGAAGTCGGGCAAGAAGATCAAGGCGGACGCGCTG 
CTCTGGTGCAACGGCCGGACCGGCAATACCGACAAGCTCGGCCTGGAGAACGTCGGCATCAAGGTCAACAGC 
CGTGGCCAGATCGAGGTGGACGAGAACTACCGCACTTCGGTGAGCAACATCTTCGCCGCCGGCGACGTGATC 
GGCTGGCCGAGCCTGGCCAGCGCTGCCTACGACCAGGGCCGCTCGGCCGCCGGCAACATCGTCGAGAGCGAC 
AGCTGGCGCTTCGTCAACGATGTGCCGACCGGCATCTACACCATTCCGGAGATCAGCTCGATCGGCAAGAAC 
GAAAGCGAACTGACCGCGGCGAAGATTCCCTACGAAGTGGGCAAGGCGTTCTTCAAGGGCATGGCCCGGGCG 
CAGATTTCCAACGAGCCGGTGGGCATGCTGAAGATCCTGTTCCATCGCGAGACTCTGGAGATCCTCGGCGTG 
CACTGCTTCGGCGACCAGGCTTCGGAAATCGTCCACATCGGCCAGGCGATCATGAACCAGCCGGGCGAGCTG 
AATACCCTGAAGTACTTCGTCAACACCACCTTCAACTACCCGACCATGGCGGAAGCCTATCGGGTAGCGGCG 
TTCGACGGCCTCAACCGGCTTTTTTGA