ViewGene
Locus Name:
GeneID:
Location:

GeneID:964
TrivialName: PA14_31990
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31990
PAO1 Ortholog Locus:PA2521
Sequence Length:1455
Protein Length:484
Start: 2785380
Stop: 2786834
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05909.1|AE004679_3 (AE004679) RND divalent metal cation efflux membrane fusion protein CzcB precursor [Pseudomonas aeruginosa PAO1]
Identities = 484/484 (100%), Positives = 484/484 (100%)
Sequence:
ATGGGCAAGAAGACGATTATCGCGGTGACCGCACTGGCACTGCTCGGCCTGGGCGGCGGCGCCTACCTGGAG
CTGGGCGGCAAGGCGCCGGCCGGCGCCGGCGAAGCGGCCGCCGAGGGCGAGGGCAGCGGACCGCGCGGCGGC
ATGTTGTTGCGCCAGGGCGACCTGACCCTGGAACTGCTGGTGCCGGAGGACGGCAGCCCGCTCAAGGCCTGG
GTCGAGCGCGGCGGCAAGGCGCTGGCGCCGCAGGAGGTGGTCCTCGGCGCGGACGTCGAGCGCGCCACCGGC
GAGGTCGAGGACCTGCTGTTCAAGGCCAGCGGCGATGGCCTGGTGGCCAATGCCGGGATCGCCGAGCCGCAT
GCCTTCACCGCGCGCCTCAAGCTGATGGCCGGCAAGCAGGGCTACGACTTCGCCTTCACCCGCGAGGAGGGC
AAGCTGGAGCTGGATGCCGAGCAGATCGAGGCCGCCGGCATCACCCTCGATACGGCGCGGACGATCAGCCTG
GCCCAGGTGGTCTCGCTGCCCGGCGAGATCCGCTTCAACGAGGACCGCACCGCGCATATCGTCCCGCGCCTG
CCGGGGATCGTCGACAGCGTGCCGGCCAACCTTGGCCAGGCGGTGAAGCAGGGCGAGCTGCTGGCGGTGATC
AGCAGCCCGCAGTTGTCCGACCAGCGCAGCGAATTCGCCGCCGCCCAGCGCCGCCTGAGCCTGGCGCAGAGC
ACCTACAAGCGCGAGCAGCAGCTGTGGAAGGAGGGCATCTCCGCGGAGCAGGAATTCCTGCTCGCCCGCCAG
GGTTTGCAGGAAGCCGAGATCGCGCTGAACAACGCGCGGGCGAAGATCGCCGCGCTCGGCGGCAATCCCAGC
CTGCAGGGCGGCAACCGCTACGAGCTGCGCGCGCCGTTCGCCGGGGTGCTGGTGGAAAAGCACCTGACCCAG
GGCGAGCCGGTGGACGGCACCGCCAACGTCTTCACCCTGTCCGACCTGTCCTCGGTCTGGGCCACCTTCAAC
GTGCCCGCGCAACTGCTCGGCCAGGTCCGCGTCGGCAGCAAGGTCAAGGTGCTCGCCCAGGCGCTGGATAGC
GAAGTGGAGGGCACCGTGTCCTACATCGGCGACCTGCTCGGCGAGCAGACCCGCGCGGCCACCGCCCGGGTC
ACCCTGAGCAACCCCGAGAGCACCTGGCGCCCGGGCCTGTTCGTTTCCGTGCAGGTGGCCGAGGCGACCCGC
AAGGAAGTCCTGACGGTCGCCGACGGCGCGGTGCAGAACGTCGACGGCGAGGACGTGGTGTTCGTCCGCGTG
GCCGACGGCTTCGTGGTCCAGCCGGTCAAGCTGGGCATCAGCGACGGCCAGCGCGTCGAGGTCCTCGAGGGC
CTGCGCGCCGGCAGCCAGGTCGCCGCCAGCGGCAGCTTCATCCTCAAATCCGAACTGGGCAAGGGCTCGGCG
GAACACGGTCACTGA
Translation:
MGKKTIIAVTALALLGLGGGAYLELGGKAPAGAGEAAAEGEGSGPRGGMLLRQGDLTLELLVPEDGSPLKAW
VERGGKALAPQEVVLGADVERATGEVEDLLFKASGDGLVANAGIAEPHAFTARLKLMAGKQGYDFAFTREEG
KLELDAEQIEAAGITLDTARTISLAQVVSLPGEIRFNEDRTAHIVPRLPGIVDSVPANLGQAVKQGELLAVI
SSPQLSDQRSEFAAAQRRLSLAQSTYKREQQLWKEGISAEQEFLLARQGLQEAEIALNNARAKIAALGGNPS
LQGGNRYELRAPFAGVLVEKHLTQGEPVDGTANVFTLSDLSSVWATFNVPAQLLGQVRVGSKVKVLAQALDS
EVEGTVSYIGDLLGEQTRAATARVTLSNPESTWRPGLFVSVQVAEATRKEVLTVADGAVQNVDGEDVVFVRV
ADGFVVQPVKLGISDGQRVEVLEGLRAGSQVAASGSFILKSELGKGSAEHGH*
AnnotationID:973GeneID:964
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 18:06:51
Gene Name:czcB
Confidence Code:2
GeneProduct:cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB famil
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0845
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK52284.1| (AY026914) cation efflux system membrane protein CzrB [Pseudomonas
           putida]
          Length = 227

 Score =  436 bits (1122), Expect = e-121
 Identities = 227/227 (100%), Positives = 227/227 (100%)

>emb|CAA67083.1| (X98451) membrane fusion protein [Ralstonia metallidurans]
          Length = 520

 Score =  434 bits (1116), Expect = e-121
 Identities = 231/445 (51%), Positives = 309/445 (69%), Gaps = 5/445 (1%)

>emb|CAD17643.1| (AL646079) PROBABLE COBALT-ZINC-CADMIUM RESISTANCE (CATION EFFLUX
           SYSTEM PROTEIN) TRANSMEMBRANE [Ralstonia solanacearum]
          Length = 404

 Score =  332 bits (852), Expect = 2e-90
 Identities = 180/339 (53%), Positives = 233/339 (68%), Gaps = 1/339 (0%)






 
Structural Features:
COG0845, AcrA, Membrane-fusion protein 
[Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  89.2% aligned
Score = 95.6 bits (236), Expect = 1e-20
Identities = 93/334 (27%), Positives = 149/334 (44%), Gaps = 9/334 (2%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1931




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19073




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33083




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:964_cdd.html


    		




 
URL:

















    ReferenceID:34526




  
Author/Investigator(s):
        Grosse C, Grass G, Anton A, Franke S, Santos AN, Lawley B, Brown NL, Nies DH.





  
Title:
        Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus.




  
PubMed:
        10198000


MedLine:
        




    
Source:
J Bacteriol. 1999 Apr;181(8):2385-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10198000









           
Homologs By Global Alignment 

           

       



     
Gene ID:964
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1707
                           PA2521_tr
                           translation of PA2521
                           484
                           99.99
                           99.99
                           0
                           2396.0
     

     
     
60581
                           gb|AAG05909.1|AE004679_3
                           (AE004679) RND divalent metal cation efflux membrane fusion protein CzcB precursor [Pseudomonas aeruginosa PAO1]
                           484
                           99.99
                           99.99
                           0
                           2396.0
     

     
     
60582
                           emb|CAB56470.1|
                           (Y14018) czrB protein [Pseudomonas aeruginosa]
                           484
                           82.85
                           83.47
                           79
                           1964.0
     

     
     
60584
                           emb|CAA67083.1|
                           (X98451) membrane fusion protein [Ralstonia metallidurans]
                           521
                           47.40
                           64.29
                           38
                           1143.0
     

     
     
60587
                           gb|AAN65678.1|AE016194_8
                           (AE016774) cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family [Pseudomonas putida KT2440]
                           486
                           47.11
                           59.87
                           72
                           1051.0
     

     






      

          
GC ORFID: 43966How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2521Glimmer Score: 

          
Start: 2168546Stop: 2167092

          
Length: 1455


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2521 translation of PA2521Homolog Bit Score 927.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6876 (ORF: BLASTX 378.0), t_PA2521 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCAAGAAGACGATTATCGCGGTGACCGCACTGGCACTGCTCGGCCTGGGCGGCGGCGCCTACCTGGAG 
CTGGGCGGCAAGGCGCCGGCCGGCGCCGGCGAAGCGGCCGCCGAGGGCGAGGGCAGCGGACCGCGCGGCGGC 
ATGTTGTTGCGCCAGGGCGACCTGACCCTGGAACTGCTGGTGCCGGAGGACGGCAGCCCGCTCAAGGCCTGG 
GTCGAGCGCGGCGGCAAGGCGCTGGCGCCGCAGGAGGTGGTCCTCGGCGCGGACGTCGAGCGCGCCACCGGC 
GAGGTCGAGGACCTGCTGTTCAAGGCCAGCGGCGATGGCCTGGTGGCCAATGCCGGGATCGCCGAGCCGCAT 
GCCTTCACCGCGCGCCTCAAGCTGATGGCCGGCAAGCAGGGCTACGACTTCGCCTTCACCCGCGAGGAGGGC 
AAGCTGGAGCTGGATGCCGAGCAGATCGAGGCCGCCGGCATCACCCTCGATACGGCGCGGACGATCAGCCTG 
GCCCAGGTGGTCTCGCTGCCCGGCGAGATCCGCTTCAACGAGGACCGCACCGCGCATATCGTCCCGCGCCTG 
CCGGGGATCGTCGACAGCGTGCCGGCCAACCTTGGCCAGGCGGTGAAGCAGGGCGAGCTGCTGGCGGTGATC 
AGCAGCCCGCAGTTGTCCGACCAGCGCAGCGAATTCGCCGCCGCCCAGCGCCGCCTGAGCCTGGCGCAGAGC 
ACCTACAAGCGCGAGCAGCAGCTGTGGAAGGAGGGCATCTCCGCGGAGCAGGAATTCCTGCTCGCCCGCCAG 
GGTTTGCAGGAAGCCGAGATCGCGCTGAACAACGCGCGGGCGAAGATCGCCGCGCTCGGCGGCAATCCCAGC 
CTGCAGGGCGGCAACCGCTACGAGCTGCGCGCGCCGTTCGCCGGGGTGCTGGTGGAAAAGCACCTGACCCAG 
GGCGAGCCGGTGGACGGCACCGCCAACGTCTTCACCCTGTCCGACCTGTCCTCGGTCTGGGCCACCTTCAAC 
GTGCCCGCGCAACTGCTCGGCCAGGTCCGCGTCGGCAGCAAGGTCAAGGTGCTCGCCCAGGCGCTGGATAGC 
GAAGTGGAGGGCACCGTGTCCTACATCGGCGACCTGCTCGGCGAGCAGACCCGCGCGGCCACCGCCCGGGTC 
ACCCTGAGCAACCCCGAGAGCACCTGGCGCCCGGGCCTGTTCGTTTCCGTGCAGGTGGCCGAGGCGACCCGC 
AAGGAAGTCCTGACGGTCGCCGACGGCGCGGTGCAGAACGTCGACGGCGAGGACGTGGTGTTCGTCCGCGTG 
GCCGACGGCTTCGTGGTCCAGCCGGTCAAGCTGGGCATCAGCGACGGCCAGCGCGTCGAGGTCCTCGAGGGC 
CTGCGCGCCGGCAGCCAGGTCGCCGCCAGCGGCAGCTTCATCCTCAAATCCGAACTGGGCAAGGGCTCGGCG 
GAACACGGTCACTGA