ViewGene
Locus Name:
GeneID:
Location:

GeneID:963
TrivialName: PA14_43970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43970
PAO1 Ortholog Locus:PA1587
Sequence Length:1437
Protein Length:478
Start: 3915093
Stop: 3913657
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04976.1|AE004586_14 (AE004586) lipoamide dehydrogenase-glc [Pseudomonas aeruginosa PAO1]
Identities = 478/478 (100%), Positives = 478/478 (100%)
Sequence:
ATGAGCCAGAAATTCGACGTGGTAGTGATTGGTGCCGGCCCCGGCGGTTACGTTGCCGCCATCCGCGCTGCC
CAGCTGGGCCTGAAGACCGCCTGCATCGAGAAGTACATCGGCAAGGAAGGCAAGGTGGCGCTCGGCGGTACC
TGCCTGAACGTTGGCTGCATCCCGTCCAAGGCGCTGCTGGACAGCTCCTACAAGTACCATGAGGCCAAAGAG
GCCTTCAAAGTCCATGGTATCGAAGCCAAGGGCGTCACCATCGACGTTCCGGCGATGGTTGCGCGCAAGGCC
AACATCGTCAAGAACCTGACCGGCGGCATCGCTACCCTGTTCAAGGCCAACGGCGTGACCTCCTTCGAAGGC
CACGGCAAGCTGCTGGCCAACAAGCAGGTGGAAGTGACCGGTCTGGACGGCAAGACCCAGGTCCTGGAAGCC
GAGAACGTGATCATCGCTTCCGGCTCCCGCCCGGTGGAGATCCCGCCGGCCCCGCTGACCGACGACATCATC
GTCGACTCCACCGGCGCCCTGGAATTCCAGGCCGTGCCGAAGAAGCTGGGTGTGATCGGCGCCGGCGTCATT
GGCCTGGAGCTGGGTTCGGTATGGGCCCGCCTGGGTGCCGAGGTGACCGTGCTGGAAGCCCTGGACAAGTTC
CTCCCGGCTGCCGACGAGCAGATCGCCAAGGAAGCCCTGAAGGTCCTGACCAAGCAAGGCCTGAACATTCGC
CTGGGCGCCCGCGTGACCGCTTCGGAAGTGAAGAAGAAGCAGGTCACCGTGACCTTCACCGATGCCAACGGC
GAGCAGAAGGAAACCTTCGACAAGCTGATCGTCGCGGTCGGCCGTCGCCCGGTGACCACCGACCTGCTGGCC
GCCGACAGCGGCGTGACCCTGGACGAGCGTGGCTTCATCTACGTCGACGACCACTGCAAGACCAGCGTTCCG
GGCGTCTTCGCCATCGGTGACGTGGTTCGCGGCGCGATGCTCGCGCACAAGGCCTCGGAAGAGGGCGTGATG
GTCGCCGAGCGCATCGCCGGCCACAAGGCGCAGATGAACTACGACCTGATCCCGTCGGTCATCTACACCCAC
CCGGAAATCGCCTGGGTCGGCAAGACCGAGCAGACCCTCAAGGCCGAGGGCGTCGAAGTCAACGTCGGCACC
TTCCCGTTCGCCGCCAGCGGTCGCGCGATGGCTGCCAACGACACCACCGGCCTGGTCAAGGTCATCGCCGAT
GCGAAGACCGACCGCGTCCTGGGCGTCCACGTGATCGGCCCGAGCGCCGCCGAGCTGGTACAGCAAGGCGCG
ATCGGCATGGAATTCGGCACCAGCGCCGAAGACCTGGGCATGATGGTCTTCTCCCACCCGACGCTGTCCGAG
GCCCTGCACGAAGCTGCCCTGGCGGTGAATGGCCATGCCATCCACATCGCCAACCGCAAGAAGCGCTGA
Translation:
MSQKFDVVVIGAGPGGYVAAIRAAQLGLKTACIEKYIGKEGKVALGGTCLNVGCIPSKALLDSSYKYHEAKE
AFKVHGIEAKGVTIDVPAMVARKANIVKNLTGGIATLFKANGVTSFEGHGKLLANKQVEVTGLDGKTQVLEA
ENVIIASGSRPVEIPPAPLTDDIIVDSTGALEFQAVPKKLGVIGAGVIGLELGSVWARLGAEVTVLEALDKF
LPAADEQIAKEALKVLTKQGLNIRLGARVTASEVKKKQVTVTFTDANGEQKETFDKLIVAVGRRPVTTDLLA
ADSGVTLDERGFIYVDDHCKTSVPGVFAIGDVVRGAMLAHKASEEGVMVAERIAGHKAQMNYDLIPSVIYTH
PEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTTGLVKVIADAKTDRVLGVHVIGPSAAELVQQGA
IGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGHAIHIANRKKR*
AnnotationID:972GeneID:963
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 06:06:53
Gene Name:lpdG
Confidence Code:2
GeneProduct:lipoamide dehydrogenase-glc
Cell Localization:()
Synonyms:lpdA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:2-oxoglutarate dehydrogenase, E3 component, lipoamide dehydrogenase
Functional Category Confidence Code:5
COGs:COG1249
Secondary Functional Category(ies):
EC Number:1.8.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA99234.1| (M28356) dihydrolipoamide dehydrogenase [Pseudomonas fluorescens]
          Length = 478

 Score =  926 bits (2392), Expect = 0.0
 Identities = 477/478 (99%), Positives = 478/478 (100%)

>gb|AAA22139.1| (M37307) lipoamide dehydrogenase [Azotobacter vinelandii]
          Length = 477

 Score =  795 bits (2053), Expect = 0.0
 Identities = 402/477 (84%), Positives = 434/477 (90%)

>gb|AAA96437.1| (M80189) lipoamide dehydrogenase [Pseudomonas putida]
          Length = 478

 Score =  792 bits (2045), Expect = 0.0
 Identities = 403/478 (84%), Positives = 436/478 (91%)








 
Structural Features:
COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex,
dihydrolipoamide dehydrogenase (E3) component, and related enzymes 
[Energy production and conversion]
CD-Length = 454 residues, 100.0% aligned
Score =  500 bits (1289), Expect = 1e-142
Identities = 229/463 (49%), Positives = 302/463 (65%), Gaps = 10/463 (2%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1929




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19071




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37245




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:963_cdd.html


    		




 
URL:

















    ReferenceID:37910




  
Author/Investigator(s):
        Palmer JA, Hatter K, Sokatch JR.





  
Title:
        Cloning and sequence analysis of the LPD-glc structural gene of Pseudomonas putida.




  
PubMed:
        1902462


MedLine:
        




    
Source:
J Bacteriol. 1991 May;173(10):3109-16.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1902462

















    ReferenceID:37911




  
Author/Investigator(s):
        Engels A, Pistorius EK.





  
Title:
        Characterization of a gene encoding dihydrolipoamide dehydrogenase of the cyanobacterium Synechocystis sp. strain PCC 6803.




  
PubMed:
        9387233


MedLine:
        




    
Source:
Microbiology. 1997 Nov;143 ( Pt 11):3543-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9387233









           
Homologs By Global Alignment 

           

       



     
Gene ID:963
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1704
                           PA1587_tr
                           translation of PA1587
                           478
                           99.99
                           99.99
                           0
                           2396.0
     

     
     
60461
                           gb|AAG04976.1|AE004586_14
                           (AE004586) lipoamide dehydrogenase-glc [Pseudomonas aeruginosa PAO1]
                           478
                           99.99
                           99.99
                           0
                           2396.0
     

     
     
60462
                           gb|AAA99234.1|
                           (M28356) dihydrolipoamide dehydrogenase [Pseudomonas fluorescens]
                           478
                           99.79
                           99.99
                           0
                           2392.0
     

     
     
60464
                           gb|AAA96437.1|
                           (M80189) lipoamide dehydrogenase [Pseudomonas putida]
                           478
                           84.30
                           91.21
                           0
                           2045.0
     

     
     
60465
                           gb|AAN69768.1|AE016613_3
                           (AE016789) 2-oxoglutarate dehydrogenase, lipoamide dehydrogenase component [Pseudomonas putida KT2440]
                           478
                           84.10
                           91.21
                           0
                           2042.0
     

     






      

          
GC ORFID: 43965How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1587Glimmer Score: 

          
Start: 1038816Stop: 1040252

          
Length: 1437


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1587 translation of PA1587Homolog Bit Score 927.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene999 (ORF: BLASTX 783.0), t_PA4829 (ORF: BLASTX 402.0), t_PA2250 (ORF: BLASTX 324.0), t_PA1587 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGAAATTCGACGTGGTAGTGATTGGTGCCGGCCCCGGCGGTTACGTTGCCGCCATCCGCGCTGCC 
CAGCTGGGCCTGAAGACCGCCTGCATCGAGAAGTACATCGGCAAGGAAGGCAAGGTGGCGCTCGGCGGTACC 
TGCCTGAACGTTGGCTGCATCCCGTCCAAGGCGCTGCTGGACAGCTCCTACAAGTACCATGAGGCCAAAGAG 
GCCTTCAAAGTCCATGGTATCGAAGCCAAGGGCGTCACCATCGACGTTCCGGCGATGGTTGCGCGCAAGGCC 
AACATCGTCAAGAACCTGACCGGCGGCATCGCTACCCTGTTCAAGGCCAACGGCGTGACCTCCTTCGAAGGC 
CACGGCAAGCTGCTGGCCAACAAGCAGGTGGAAGTGACCGGTCTGGACGGCAAGACCCAGGTCCTGGAAGCC 
GAGAACGTGATCATCGCTTCCGGCTCCCGCCCGGTGGAGATCCCGCCGGCCCCGCTGACCGACGACATCATC 
GTCGACTCCACCGGCGCCCTGGAATTCCAGGCCGTGCCGAAGAAGCTGGGTGTGATCGGCGCCGGCGTCATT 
GGCCTGGAGCTGGGTTCGGTATGGGCCCGCCTGGGTGCCGAGGTGACCGTGCTGGAAGCCCTGGACAAGTTC 
CTCCCGGCTGCCGACGAGCAGATCGCCAAGGAAGCCCTGAAGGTCCTGACCAAGCAAGGCCTGAACATTCGC 
CTGGGCGCCCGCGTGACCGCTTCGGAAGTGAAGAAGAAGCAGGTCACCGTGACCTTCACCGATGCCAACGGC 
GAGCAGAAGGAAACCTTCGACAAGCTGATCGTCGCGGTCGGCCGTCGCCCGGTGACCACCGACCTGCTGGCC 
GCCGACAGCGGCGTGACCCTGGACGAGCGTGGCTTCATCTACGTCGACGACCACTGCAAGACCAGCGTTCCG 
GGCGTCTTCGCCATCGGTGACGTGGTTCGCGGCGCGATGCTCGCGCACAAGGCCTCGGAAGAGGGCGTGATG 
GTCGCCGAGCGCATCGCCGGCCACAAGGCGCAGATGAACTACGACCTGATCCCGTCGGTCATCTACACCCAC 
CCGGAAATCGCCTGGGTCGGCAAGACCGAGCAGACCCTCAAGGCCGAGGGCGTCGAAGTCAACGTCGGCACC 
TTCCCGTTCGCCGCCAGCGGTCGCGCGATGGCTGCCAACGACACCACCGGCCTGGTCAAGGTCATCGCCGAT 
GCGAAGACCGACCGCGTCCTGGGCGTCCACGTGATCGGCCCGAGCGCCGCCGAGCTGGTACAGCAAGGCGCG 
ATCGGCATGGAATTCGGCACCAGCGCCGAAGACCTGGGCATGATGGTCTTCTCCCACCCGACGCTGTCCGAG 
GCCCTGCACGAAGCTGCCCTGGCGGTGAATGGCCATGCCATCCACATCGCCAACCGCAAGAAGCGCTGA