ViewGene
Locus Name:
GeneID:
Location:

GeneID:96
TrivialName: PA14_64230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64230
PAO1 Ortholog Locus:PA4856
Sequence Length:2829
Protein Length:942
Start: 5722815
Stop: 5725643
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08241.1|AE004899_2 (AE004899) probable sensor/response regulator hybrid [Pseudomonas aeruginosa PAO1]
Identities = 941/942 (99%), Positives = 942/942 (100%)
Sequence:
GTGGTACGGCTTCGGATCGCCATAGGACTACTCGTCAGCTTCCTGCTGCTTCTGCTCGGTCCGATGTCGCCG
GCGGTCGCGGACGACGCCGGCGTTTCCAGCGTTCCCCTGCAAACCACCGCCACTACTCCCAGCGCCAACCAG
AACTGGCGCCTGCTGCGCGACGAGAGCGCGCAACTGCGGATCGCCGACGTCCTCCAGCGCAAGGAGCAGTTC
CGCCCGCTGGCCAAGCGCTCCTTCATCTTCCCCGCCAGCCCCCAGGCGGTCTGGCTGCAGGTCCAGCTTCCC
GCGCAGAAGGTGCCGAGCTGGCTGTGGATCTTCGCCCCGCGGGTGCAGTATCTGGACTACTACCTGGTGCAG
GACGGCCAACTGGTGCGGGACCAGCACACCGGCGAGTCGCGACCGTTCCAGGAGCGCCCGCTGCCCTCGCGC
TCCTACCTGTTCTCGCTGCCGGTCGACGGCAAGCCGATGACCCTGTACGTGCGGATGACCTCCAACCATCCA
CTGATGGCCTGGTTCGACCAGATCGACGAAGCCGGCCTGGTCGGCCTGGAGAAGCCCGCCTACGCCTTCGGC
ATGCTGCTCGGCGGCATGCTCCTGCTGCTGATGTACAACCTGATCCGCTTCGCCTACTCGCGCAGCGCCAGC
AGCCTGTGGCTGGCGGCGGTGCACGCGGCGCTGGCGGTCTGCGCCGCGGCGAACCTGGGGCTGGTGGCGTTC
TGGCTGCCGGGACTCAAGTTCAACCAGTCGCTGACCGCCGACCTCGGCGCCCTCGGCGCCGCGGTGAGCCTG
CTGTGGTTCGCCTGCAGCTTCTTCCGCGGCACTGCGGAAAGCCGCCTGAACCGCCTCCTGCAAGGCGAGGCG
CTGTTGATCCTGGCGGTCGGCGCGATCATCGCCTTCACCCAGCAACTCTGGTTCAGCTGGCTGATCTACCTG
CTGGTGATCCTCAGTTCGCTCAGCGTCCCGCTGGTCGCGGCCTGGCACTGGTATCGCGGCTACCAGCCGGCG
CGCCTGATCGTTGCCGGGATGATCGTGTTCAACGCCGGCTTCATGGTGTTCCTGCCGGTACTGTTCGGCACC
AAGCAACTCGATCCGGGCTGGCTGGTGCTCGGCGTGTTCAGCTTCGCCACCCTCGCCGGGCTGGTCCTGAGC
GTCTCGCTCACCGAACGCCAGCGGCTGATCCAGCAGCTCAACCTGCAACAGCGCACCAGCGAGGCGGCGCAC
ACCGCGGAATTGCAGACCAAGGCCGAGTTCCTGGCCAAGATCAGCCACGAGATCCGCACGCCCATGAACGGC
GTGCTGGGGATGACCGAACTGCTCCTCGGCACCCCGTTGTCGGCGAAGCAGCGCGACTACGTGCAGACCATC
CACAGCGCCGGCAACGAGCTGCTCACGCTGATCAACGAGATCCTCGACATCTCCAAGCTGGAGTCCGGGCAG
ATCGAGCTGGACGAAGTGCAGTTCGACCTCAACGCGCTGATCGAGGACTGCCTGGATATCTTCCGGGTCAAG
GCCGAGCAGCAGCGCATCGAGCTGATCAGCTTCACCCAGCCGCAGGTGCCGCGGGTCATCGGCGGCGATCCG
ACGCGCCTGCGCCAGGTGGTCCTGAGCCTGCTGGACAACGCGTTCAAGCAGACCGAGGAAGGCGAGATCCTG
CTGGTGGTGGCCCTCGACGACCAGGGCGAGACGCCACGCCTGCGCATCGCCGTGCAGGACAGCGGCCACCCG
TTCGACGCCAAGGAGCGCGAGGCGCTGCTCACCGCCGAGCTGCACAGCGGCGACTTCCTTTCCGCCAGCAAG
CTCGGCAGCCACCTCGGCCTGATCATCGCCCGCCAACTGGTGCGGCTGATGGGCGGGGAGTTCGGCATCCAG
TCCGGCAGCAGCCAGGGCACCACCCTGTCGCTGACCCTGCCGCTCGACCCGCAGCAACTGGAGAACCCCACC
GCCGACCTCGACGGCCCGCTGCAGGGCGCCCGGCTGCTGGTGGTGGACGACAACGAAACCTGCCGCAAGGTG
CTAGTGCAGCAGTGCAGCGGCTGGGGCCTGAACGTCAGCGCGGTGTCGTCCGGCAAGGAGGCGCTGGCCCAG
TTGCGCACCAAGGCGCACCTGCGCGAGTACTTCGACGTGGTCCTGCTCGACCAGGACATGCCCGGCATGACC
GGCATGCAACTGGCGGCGAAGATCAAGGAAGACCCCAACCTCAACCACGACATCCTGCTGATCATGCTCACC
GGGATCAGCAACGCGCCGAGCAAGATCATCGCCCGCAACGCCGGGATCAAGCGCATCCTGGCCAAGCCGGTG
GCCGGCTACACGCTGAAGGCGACCCTCGCCGACGAACTCGCCCAGCGCGGCGTCAGCGGCGTCACCAACTAC
CTGCAACCGGCCAAGGAAGCGCAGGCGCCGAGCCTGCCGAGCGATTTCCGGATCCTCGTCGCCGAGGACAAC
AGCATCTCCACCAAGGTCATCCGCGGCATGTTGAACAAGCTCAACCTGCAGCCGGACACCGCCAGCAACGGC
CAGGAGGCGCTCAGCGCGATGAAGGCGACCCAGTACGACCTGGTGCTGATGGACTGCGAGATGCCGGTGCTC
GACGGCTTCTCCGCCACCGAACAGCTACGCGCCTGGGAAGCCCACGAACAGCGCCCGCATACCCCGGTGGTG
GCGCTCACCGCGCACATCCTCAGCGAACACAAGGAACGCGCGCGGCTGGTCGGCATGGACGGGCACATGGCC
AAGCCGGTGGAGCTGTCGCAATTGCGTGAGCTGATCGCCTACTGGGTCGGCGAACGCGATCGCCGCCGCCAG
GGCGACGCCCTGCCCTCCTGA
Translation:
MVRLRIAIGLLVSFLLLLLGPMSPAVADDAGVSSVPLQTTATTPSANQNWRLLRDESAQLRIADVLQRKEQF
RPLAKRSFIFPASPQAVWLQVQLPAQKVPSWLWIFAPRVQYLDYYLVQDGQLVRDQHTGESRPFQERPLPSR
SYLFSLPVDGKPMTLYVRMTSNHPLMAWFDQIDEAGLVGLEKPAYAFGMLLGGMLLLLMYNLIRFAYSRSAS
SLWLAAVHAALAVCAAANLGLVAFWLPGLKFNQSLTADLGALGAAVSLLWFACSFFRGTAESRLNRLLQGEA
LLILAVGAIIAFTQQLWFSWLIYLLVILSSLSVPLVAAWHWYRGYQPARLIVAGMIVFNAGFMVFLPVLFGT
KQLDPGWLVLGVFSFATLAGLVLSVSLTERQRLIQQLNLQQRTSEAAHTAELQTKAEFLAKISHEIRTPMNG
VLGMTELLLGTPLSAKQRDYVQTIHSAGNELLTLINEILDISKLESGQIELDEVQFDLNALIEDCLDIFRVK
AEQQRIELISFTQPQVPRVIGGDPTRLRQVVLSLLDNAFKQTEEGEILLVVALDDQGETPRLRIAVQDSGHP
FDAKEREALLTAELHSGDFLSASKLGSHLGLIIARQLVRLMGGEFGIQSGSSQGTTLSLTLPLDPQQLENPT
ADLDGPLQGARLLVVDDNETCRKVLVQQCSGWGLNVSAVSSGKEALAQLRTKAHLREYFDVVLLDQDMPGMT
GMQLAAKIKEDPNLNHDILLIMLTGISNAPSKIIARNAGIKRILAKPVAGYTLKATLADELAQRGVSGVTNY
LQPAKEAQAPSLPSDFRILVAEDNSISTKVIRGMLNKLNLQPDTASNGQEALSAMKATQYDLVLMDCEMPVL
DGFSATEQLRAWEAHEQRPHTPVVALTAHILSEHKERARLVGMDGHMAKPVELSQLRELIAYWVGERDRRRQ
GDALPS*
AnnotationID:106GeneID:96
AnnotatorUID: danlee
Modification Date/Time: 2006-01-27 15:03:44
Gene Name:retS
Confidence Code:1
GeneProduct:RetS (Regulator of Exopolysaccharide and Type III Secretion)
Cell Localization:()
Synonyms:RtsM
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0642
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70393.1|AE016681_2 (AE016792) sensor histidine kinase/response regulator [Pseudomonas
           putida KT2440]
          Length = 923

 Score = 1021 bits (2640), Expect = 0.0
 Identities = 555/936 (59%), Positives = 659/936 (70%), Gaps = 22/936 (2%)

>gb|AAO58297.1| (AE016873) sensor histidine kinase/response regulator [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 929

 Score =  966 bits (2496), Expect = 0.0
 Identities = 526/914 (57%), Positives = 646/914 (70%), Gaps = 6/914 (0%)








 
Structural Features:
COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms]
CD-Length = 336 residues,  99.1% aligned
Score =  145 bits (366), Expect = 2e-35
Identities = 103/344 (29%), Positives = 168/344 (48%), Gaps = 12/344 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14053




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17337




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35199




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:96_cdd.html


    		




 
URL:

















    ReferenceID:38276




  
Author/Investigator(s):
        Goodman AL, Kulasekara B, Rietsch A, Boyd D, Smith RS, Lory S.





  
Title:
        A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa.




  
PubMed:
        15525535


MedLine:
        




    
Source:
Dev Cell. 2004 Nov;7(5):745-54.






    
Reference Type: 







    
Data:


    		




 
URL:

















    ReferenceID:38277




  
Author/Investigator(s):
        Laskowski MA, Osborn E, Kazmierczak B





  
Title:
        A novel sensor kinase-response regulator hybrid regulates type III secretion and is required for virulence in Pseudomonas aeruginosa.




  
PubMed:
        15522089


MedLine:
        




    
Source:
Mol Microbiol. 2004 Nov;54(4):1090-103.






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:96
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
180
                           PA4856_tr
                           translation of PA4856
                           942
                           99.89
                           99.99
                           0
                           4757.0
     

     
     
14447
                           gb|AAG08241.1|AE004899_2
                           (AE004899) probable sensor/response regulator hybrid [Pseudomonas aeruginosa PAO1]
                           942
                           99.78
                           99.99
                           0
                           4754.0
     

     
     
14448
                           gb|AAN70393.1|AE016681_2
                           (AE016792) sensor histidine kinase/response regulator [Pseudomonas putida KT2440]
                           956
                           58.57
                           69.76
                           47
                           2649.0
     

     
     
14449
                           gb|AAO58297.1|
                           (AE016873) sensor histidine kinase/response regulator [Pseudomonas syringae pv. tomato str. DC3000]
                           963
                           56.49
                           69.26
                           55
                           2515.0
     

     
     
181
                           PA3974_tr
                           translation of PA3974
                           995
                           25.62
                           39.29
                           253
                           702.0
     

     






      

          
GC ORFID: 43098How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4856Glimmer Score: 

          
Start: 4986495Stop: 4989323

          
Length: 2829


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4856 translation of PA4856Homolog Bit Score 1837.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5778 (ORF: BLASTX 971.0), t_PA4856 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGTACGGCTTCGGATCGCCATAGGACTACTCGTCAGCTTCCTGCTGCTTCTGCTCGGTCCGATGTCGCCG 
GCGGTCGCGGACGACGCCGGCGTTTCCAGCGTTCCCCTGCAAACCACCGCCACTACTCCCAGCGCCAACCAG 
AACTGGCGCCTGCTGCGCGACGAGAGCGCGCAACTGCGGATCGCCGACGTCCTCCAGCGCAAGGAGCAGTTC 
CGCCCGCTGGCCAAGCGCTCCTTCATCTTCCCCGCCAGCCCCCAGGCGGTCTGGCTGCAGGTCCAGCTTCCC 
GCGCAGAAGGTGCCGAGCTGGCTGTGGATCTTCGCCCCGCGGGTGCAGTATCTGGACTACTACCTGGTGCAG 
GACGGCCAACTGGTGCGGGACCAGCACACCGGCGAGTCGCGACCGTTCCAGGAGCGCCCGCTGCCCTCGCGC 
TCCTACCTGTTCTCGCTGCCGGTCGACGGCAAGCCGATGACCCTGTACGTGCGGATGACCTCCAACCATCCA 
CTGATGGCCTGGTTCGACCAGATCGACGAAGCCGGCCTGGTCGGCCTGGAGAAGCCCGCCTACGCCTTCGGC 
ATGCTGCTCGGCGGCATGCTCCTGCTGCTGATGTACAACCTGATCCGCTTCGCCTACTCGCGCAGCGCCAGC 
AGCCTGTGGCTGGCGGCGGTGCACGCGGCGCTGGCGGTCTGCGCCGCGGCGAACCTGGGGCTGGTGGCGTTC 
TGGCTGCCGGGACTCAAGTTCAACCAGTCGCTGACCGCCGACCTCGGCGCCCTCGGCGCCGCGGTGAGCCTG 
CTGTGGTTCGCCTGCAGCTTCTTCCGCGGCACTGCGGAAAGCCGCCTGAACCGCCTCCTGCAAGGCGAGGCG 
CTGTTGATCCTGGCGGTCGGCGCGATCATCGCCTTCACCCAGCAACTCTGGTTCAGCTGGCTGATCTACCTG 
CTGGTGATCCTCAGTTCGCTCAGCGTCCCGCTGGTCGCGGCCTGGCACTGGTATCGCGGCTACCAGCCGGCG 
CGCCTGATCGTTGCCGGGATGATCGTGTTCAACGCCGGCTTCATGGTGTTCCTGCCGGTACTGTTCGGCACC 
AAGCAACTCGATCCGGGCTGGCTGGTGCTCGGCGTGTTCAGCTTCGCCACCCTCGCCGGGCTGGTCCTGAGC 
GTCTCGCTCACCGAACGCCAGCGGCTGATCCAGCAGCTCAACCTGCAACAGCGCACCAGCGAGGCGGCGCAC 
ACCGCGGAATTGCAGACCAAGGCCGAGTTCCTGGCCAAGATCAGCCACGAGATCCGCACGCCCATGAACGGC 
GTGCTGGGGATGACCGAACTGCTCCTCGGCACCCCGTTGTCGGCGAAGCAGCGCGACTACGTGCAGACCATC 
CACAGCGCCGGCAACGAGCTGCTCACGCTGATCAACGAGATCCTCGACATCTCCAAGCTGGAGTCCGGGCAG 
ATCGAGCTGGACGAAGTGCAGTTCGACCTCAACGCGCTGATCGAGGACTGCCTGGATATCTTCCGGGTCAAG 
GCCGAGCAGCAGCGCATCGAGCTGATCAGCTTCACCCAGCCGCAGGTGCCGCGGGTCATCGGCGGCGATCCG 
ACGCGCCTGCGCCAGGTGGTCCTGAGCCTGCTGGACAACGCGTTCAAGCAGACCGAGGAAGGCGAGATCCTG 
CTGGTGGTGGCCCTCGACGACCAGGGCGAGACGCCACGCCTGCGCATCGCCGTGCAGGACAGCGGCCACCCG 
TTCGACGCCAAGGAGCGCGAGGCGCTGCTCACCGCCGAGCTGCACAGCGGCGACTTCCTTTCCGCCAGCAAG 
CTCGGCAGCCACCTCGGCCTGATCATCGCCCGCCAACTGGTGCGGCTGATGGGCGGGGAGTTCGGCATCCAG 
TCCGGCAGCAGCCAGGGCACCACCCTGTCGCTGACCCTGCCGCTCGACCCGCAGCAACTGGAGAACCCCACC 
GCCGACCTCGACGGCCCGCTGCAGGGCGCCCGGCTGCTGGTGGTGGACGACAACGAAACCTGCCGCAAGGTG 
CTAGTGCAGCAGTGCAGCGGCTGGGGCCTGAACGTCAGCGCGGTGTCGTCCGGCAAGGAGGCGCTGGCCCAG 
TTGCGCACCAAGGCGCACCTGCGCGAGTACTTCGACGTGGTCCTGCTCGACCAGGACATGCCCGGCATGACC 
GGCATGCAACTGGCGGCGAAGATCAAGGAAGACCCCAACCTCAACCACGACATCCTGCTGATCATGCTCACC 
GGGATCAGCAACGCGCCGAGCAAGATCATCGCCCGCAACGCCGGGATCAAGCGCATCCTGGCCAAGCCGGTG 
GCCGGCTACACGCTGAAGGCGACCCTCGCCGACGAACTCGCCCAGCGCGGCGTCAGCGGCGTCACCAACTAC 
CTGCAACCGGCCAAGGAAGCGCAGGCGCCGAGCCTGCCGAGCGATTTCCGGATCCTCGTCGCCGAGGACAAC 
AGCATCTCCACCAAGGTCATCCGCGGCATGTTGAACAAGCTCAACCTGCAGCCGGACACCGCCAGCAACGGC 
CAGGAGGCGCTCAGCGCGATGAAGGCGACCCAGTACGACCTGGTGCTGATGGACTGCGAGATGCCGGTGCTC 
GACGGCTTCTCCGCCACCGAACAGCTACGCGCCTGGGAAGCCCACGAACAGCGCCCGCATACCCCGGTGGTG 
GCGCTCACCGCGCACATCCTCAGCGAACACAAGGAACGCGCGCGGCTGGTCGGCATGGACGGGCACATGGCC 
AAGCCGGTGGAGCTGTCGCAATTGCGTGAGCTGATCGCCTACTGGGTCGGCGAACGCGATCGCCGCCGCCAG 
GGCGACGCCCTGCCCTCCTGA