ViewGene
Locus Name:
GeneID:
Location:

GeneID:959
TrivialName: PA14_33500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_33500
PAO1 Ortholog Locus:PA2413
Sequence Length:1410
Protein Length:469
Start: 2947542
Stop: 2948951
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05801.1|AE004668_11 (AE004668) probable class III aminotransferase [Pseudomonas aeruginosa PAO1]
Identities = 469/469 (100%), Positives = 469/469 (100%)
Sequence:
ATGCACGTCGCCACCAGCGTCATCGAAGACCAGCCGCTCCGCGCCACCCCGGCGGAGGGCGAAACGCTCTAC
GAGTTCTCCCAGTCGCCCTTGCTGGAACGGCAAAGCCGCCAGGAGTCCAACGCGCGCAGCTATCCGCGGCGG
ATTCCGCTGGCGCTGAAGAAGGCCAGGGGGCTGCTGGTGGAGGACGTCGAGGGGCGTACCTTCATCGATTGC
CTGGCCGGCGCCGGCACCCTGGCGCTGGGGCACAACCATCCGGTGGTGATCGAGGCGATCCGCCAGGTTCTG
GCCGACGAGCTGCCGCTGCACACCCTCGACCTGACCACCCCGGTGAAGGACCAGTTCGTCCAGGACCTGTTC
GGCCTGCTGCCGCCGGCGCTGGCCGCCGAGGCGAAGATCCAGTTCTGCGGCCCGACCGGCACCGACGCGGTG
GAAGCCGCGCTGAAACTGGTACGCACCGCCACCGGGCGCAGCACCATCCTGTCGTTCCAGGGCGGCTACCAC
GGCATGAGCCAGGGCGCGCTGGGGCTGATGGGCAATCTCGGACCGAAGAAGCCGCTGGGCGCGGTGCTCAGC
ACGGGCGTGCAGTTCCTGCCGTATCCCTACGATTACCGTTGCCCGTTCGGTCTCGGCGGCGAGGCGGGGGTC
AAGGCGAACCTGCACTACCTGGAGAACCTGCTGAACGATCCGGAAGGCGGCGTGCAGTTGCCGGCGGCGGTG
ATCGTCGAGGTGGTCCAGGGCGAGGGCGGCGTGGTCCCGGCCGATCTCGACTGGCTGCGCGGCTTGCGGCGG
ATCACCGAGCAGGCCGGGGTGGCGCTGATCGTCGACGAGATCCAGAGCGGCTTCGCCCGTACCGGCAGGATG
TTCGCCTTCGAGCACGCCGGCATCGTCCCCGACGTGGTGGTGCTGTCCAAGGCCATCGGCGGCAGCCTGCCG
CTGGCGGTGGTGGTCTACCGCGAATGGCTGGACAAATGGCAGCCCGGCGCCCACGCCGGCACCTTCCGCGGC
AACCAGATGGCCATGGCCGCCGGTTCGGCGGTGATGCGCTACCTGAAGGAACACGACCTGGCGGCCCACGCG
GCGGCCATGGGCGAGCGCCTGGCCGAGCACCTGCGCATCCTCCAGCGCGACTATCCGCAACTGGGCGATATC
CGCGGTCGGGGGCTGATGCTCGGGGTCGAGATCGTCGACCCGCAGGGCGAGGCGGATGCCCTCGGTCATCCG
CCGACCGACGGCGCCCTGGCCTCGCGGGTCCAGCGCGAGTGCCTGAGGCGCGGCCTGATCCTCGAACTGGGC
GGCCGCCACGGCAGCGTGGTGCGCTTCCTGCCGCCGCTGATCATCGGCGCCGAACAGATCGACGAGGTGGCC
CGGCGTTTCGCCCGCGCCCTCGGCGCGGCGCTGGCCGGCTAA
Translation:
MHVATSVIEDQPLRATPAEGETLYEFSQSPLLERQSRQESNARSYPRRIPLALKKARGLLVEDVEGRTFIDC
LAGAGTLALGHNHPVVIEAIRQVLADELPLHTLDLTTPVKDQFVQDLFGLLPPALAAEAKIQFCGPTGTDAV
EAALKLVRTATGRSTILSFQGGYHGMSQGALGLMGNLGPKKPLGAVLSTGVQFLPYPYDYRCPFGLGGEAGV
KANLHYLENLLNDPEGGVQLPAAVIVEVVQGEGGVVPADLDWLRGLRRITEQAGVALIVDEIQSGFARTGRM
FAFEHAGIVPDVVVLSKAIGGSLPLAVVVYREWLDKWQPGAHAGTFRGNQMAMAAGSAVMRYLKEHDLAAHA
AAMGERLAEHLRILQRDYPQLGDIRGRGLMLGVEIVDPQGEADALGHPPTDGALASRVQRECLRRGLILELG
GRHGSVVRFLPPLIIGAEQIDEVARRFARALGAALAG*
AnnotationID:968GeneID:959
AnnotatorUID: danlee
Modification Date/Time: 2006-01-30 11:11:43
Gene Name:pvdH
Confidence Code:1
GeneProduct:L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase, PvdH
Cell Localization:(1) Cytoplasmic
Synonyms:daT
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:2,4-diaminobutyrate 4-transaminase
Functional Category Confidence Code:5
COGs:COG0160,pfam00202
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:
Pyoverdine synthesis
Homology:
>gb|AAO55653.1| (AE016863) 2,4-diaminobutyrate 4-transaminase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 488

 Score =  797 bits (2059), Expect = 0.0
 Identities = 394/468 (84%), Positives = 426/468 (91%)

>gb|AAN69804.1|AE016619_2 (AE016789) diaminobutyrate-2-oxoglutarate transaminase [Pseudomonas
           putida KT2440]
          Length = 452

 Score =  763 bits (1969), Expect = 0.0
 Identities = 374/447 (83%), Positives = 413/447 (92%)

>dbj|BAC70178.1| (AP005031) putative class III aminotransferase [Streptomyces
           avermitilis MA-4680]
          Length = 484

 Score =  496 bits (1277), Expect = e-139
 Identities = 256/454 (56%), Positives = 316/454 (69%), Gaps = 24/454 (5%)
Structural Features:
COG0160, GabT, 4-aminobutyrate aminotransferase and related aminotransferases 
[Amino acid transport and metabolism]
CD-Length = 447 residues,  95.7% aligned
Score =  488 bits (1258), Expect = 6e-139
Identities = 190/439 (43%), Positives = 264/439 (60%), Gaps = 12/439 (2%)

pfam00202, Aminotran_3, Aminotransferase class-III.
CD-Length = 402 residues, 100.0% aligned
Score =  332 bits (853), Expect = 5e-92
Identities = 156/430 (36%), Positives = 230/430 (53%), Gaps = 35/430 (8%)
Genomic Context:






   
Comment:


















    ReferenceID:1921




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19063




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32959




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:959_cdd.html


    		




 
URL:

















    ReferenceID:38112




  
Author/Investigator(s):
        Vandenende CS, Vlasschaert M, Seah SY.





  
Title:
        Functional characterization of an aminotransferase required for pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa PAO1.




  
PubMed:
        15317763


MedLine:
        




    
Source:
J Bacteriol. 2004 Sep;186(17):5596-602.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=15317763

















    ReferenceID:38113




  
Author/Investigator(s):
        Ikai H, Yamamoto S.





  
Title:
        Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii.




  
PubMed:
        9260954


MedLine:
        




    
Source:
J Bacteriol. 1997 Aug;179(16):5118-25.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9260954

















    ReferenceID:38114




  
Author/Investigator(s):
        Lamont IL, Martin LW.





  
Title:
        Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.




  
PubMed:
        12686626


MedLine:
        




    
Source:
Microbiology. 2003 Apr;149(Pt 4):833-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12686626&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:959
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1700
                           PA2413_tr
                           translation of PA2413
                           469
                           99.99
                           99.99
                           0
                           2401.0
     

     
     
60379
                           gb|AAG05801.1|AE004668_11
                           (AE004668) probable class III aminotransferase [Pseudomonas aeruginosa PAO1]
                           469
                           99.99
                           99.99
                           0
                           2401.0
     

     
     
60380
                           gb|AAO55653.1|
                           (AE016863) 2,4-diaminobutyrate 4-transaminase [Pseudomonas syringae pv. tomato str. DC3000]
                           488
                           80.73
                           87.29
                           19
                           2057.0
     

     
     
60381
                           gb|AAN69804.1|AE016619_2
                           (AE016789) diaminobutyrate-2-oxoglutarate transaminase [Pseudomonas putida KT2440]
                           470
                           79.57
                           87.87
                           19
                           1965.0
     

     
     
60383
                           dbj|BAC90164.1|
                           (AP006575) diaminobutyrate--pyruvate transaminase [Gloeobacter violaceus PCC 7421]
                           490
                           59.38
                           70.81
                           28
                           1457.0
     

     






      

          
GC ORFID: 43961How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2413Glimmer Score: 

          
Start: 2006374Stop: 2004965

          
Length: 1410


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2413 translation of PA2413Homolog Bit Score 929.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene935 (ORF: BLASTX 797.0), psyr_15may02_Scaffold1_revised_gene1460 (ORF: BLASTX 510.0), psyr_15may02_Scaffold7_revised_gene7129 (ORF: BLASTX 451.0), t_PA2413 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCACGTCGCCACCAGCGTCATCGAAGACCAGCCGCTCCGCGCCACCCCGGCGGAGGGCGAAACGCTCTAC 
GAGTTCTCCCAGTCGCCCTTGCTGGAACGGCAAAGCCGCCAGGAGTCCAACGCGCGCAGCTATCCGCGGCGG 
ATTCCGCTGGCGCTGAAGAAGGCCAGGGGGCTGCTGGTGGAGGACGTCGAGGGGCGTACCTTCATCGATTGC 
CTGGCCGGCGCCGGCACCCTGGCGCTGGGGCACAACCATCCGGTGGTGATCGAGGCGATCCGCCAGGTTCTG 
GCCGACGAGCTGCCGCTGCACACCCTCGACCTGACCACCCCGGTGAAGGACCAGTTCGTCCAGGACCTGTTC 
GGCCTGCTGCCGCCGGCGCTGGCCGCCGAGGCGAAGATCCAGTTCTGCGGCCCGACCGGCACCGACGCGGTG 
GAAGCCGCGCTGAAACTGGTACGCACCGCCACCGGGCGCAGCACCATCCTGTCGTTCCAGGGCGGCTACCAC 
GGCATGAGCCAGGGCGCGCTGGGGCTGATGGGCAATCTCGGACCGAAGAAGCCGCTGGGCGCGGTGCTCAGC 
ACGGGCGTGCAGTTCCTGCCGTATCCCTACGATTACCGTTGCCCGTTCGGTCTCGGCGGCGAGGCGGGGGTC 
AAGGCGAACCTGCACTACCTGGAGAACCTGCTGAACGATCCGGAAGGCGGCGTGCAGTTGCCGGCGGCGGTG 
ATCGTCGAGGTGGTCCAGGGCGAGGGCGGCGTGGTCCCGGCCGATCTCGACTGGCTGCGCGGCTTGCGGCGG 
ATCACCGAGCAGGCCGGGGTGGCGCTGATCGTCGACGAGATCCAGAGCGGCTTCGCCCGTACCGGCAGGATG 
TTCGCCTTCGAGCACGCCGGCATCGTCCCCGACGTGGTGGTGCTGTCCAAGGCCATCGGCGGCAGCCTGCCG 
CTGGCGGTGGTGGTCTACCGCGAATGGCTGGACAAATGGCAGCCCGGCGCCCACGCCGGCACCTTCCGCGGC 
AACCAGATGGCCATGGCCGCCGGTTCGGCGGTGATGCGCTACCTGAAGGAACACGACCTGGCGGCCCACGCG 
GCGGCCATGGGCGAGCGCCTGGCCGAGCACCTGCGCATCCTCCAGCGCGACTATCCGCAACTGGGCGATATC 
CGCGGTCGGGGGCTGATGCTCGGGGTCGAGATCGTCGACCCGCAGGGCGAGGCGGATGCCCTCGGTCATCCG 
CCGACCGACGGCGCCCTGGCCTCGCGGGTCCAGCGCGAGTGCCTGAGGCGCGGCCTGATCCTCGAACTGGGC 
GGCCGCCACGGCAGCGTGGTGCGCTTCCTGCCGCCGCTGATCATCGGCGCCGAACAGATCGACGAGGTGGCC 
CGGCGTTTCGCCCGCGCCCTCGGCGCGGCGCTGGCCGGCTAA