ViewGene
Locus Name:
GeneID:
Location:

GeneID:949
TrivialName: PA14_44470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44470
PAO1 Ortholog Locus:PA1546
Sequence Length:1383
Protein Length:460
Start: 3959575
Stop: 3960957
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04935.1|AE004582_13 (AE004582) oxygen-independent coproporphyrinogen III oxidase [Pseudomonas aeruginosa PAO1]
Identities = 460/460 (100%), Positives = 460/460 (100%)
Sequence:
ATGCTCGACACCATTCGCTGGGACGCTGACCTGATTCGGCGCTACGACCTTTCCGGCCCGCGTTACACCTCC
TATCCGACCGCCGTGCAGTTCCACGAAGGCATCGGCCCGTTCGACCAGTTGCACGCCCTGCGCGACAGCCGG
AAGGCCGGACACCCCCTGTCGCTCTACGTGCACATCCCGTTCTGCGCCAACATCTGCTACTACTGCGCCTGC
AACAAGGTGATCACCAAGGATCGCGGGCGCAGCGCGCCGTACCTCGCGCGCCTGGTCCGCGAGATCGAGATC
GTCAGCCGCCACCTCTCCCGCGAGCAGGTGGTCGAGCAACTGCACTTCGGTGGCGGAACCCCGACCTTCCTC
AGTCCCGGCCAGTTGCGCGAACTCATGTCGCAATTGCGCACCCACCTCAACCTGCTGGACGACGATTCGGGC
GACTACGGCATCGAGATCGACCCACGCGAGGCCGACTGGTCGACCATGGGCCTGCTCCGCGAACTCGGTTTC
AACCGCGTCAGCCTCGGCGTACAGGACTTCGACATGGAGGTGCAGAAGGCGGTCAACCGCATGCAGACCCCG
GAGGAGACCCGTACCATCGTCGAGGCCGCGCGCACCCTGCAGTACCGCTCGATCAACCTCGACCTGATCTAC
GGACTGCCGAAACAGACGCCGGACAGCTTCGCCCGCACGGTCGACGAAGTGATCGCGCTACAGCCGGATCGC
CTCTCGGTGTTCAACTACGCCCACCTGCCCGAGCGTTTCATGCCGCAGCGGCGGATCAACGCCGACGACCTG
CCGAGCCCGGGCCAGAAGCTGGAAATGCTCCAGCGCACCACCGAGCAACTCGCCGCGGCGGGCTACCGCTAT
ATCGGCATGGATCACTTCGCCCTGCCCGACGACGAGCTGGCCAGCGCCCAGGAAGACGGCACCCTGCAACGC
AATTTCCAGGGCTACACCACGCACGGGCACTGTGACCTGGTAGGTCTCGGGGTCTCGGCGATCAGCCAGATC
GGCGACCTCTACAGCCAGAACAGCAGCGACATCAACGACTACCAGACCAGCCTCGACAACGGCCAGTTGGCG
ATCCGTCGCGGCCTGCACTGCAACAGCGACGACCGGGTGCGGCGCGCGGTGATCCAGCAACTGATCTGCCAC
TTTGAACTGGCCTTCGAGGATATCGAGACGGAGTTCGGCATCGACTTCCGCAGCTACTTCGCCGAGCTGTGG
CCCGACCTCGAACGCTTCGCCGCCGACGGCCTGATCCGCCTGGACGCCAAGGGCATCGACATCACCTCCTCC
GGGCGTCTGCTGGTGCGTTCGATCTGCATGCTGTTCGACCGCTACCTGCCCAGCCTCAACCGGCAGCGCTTC
TCCCGGGTGATCTGA
Translation:
MLDTIRWDADLIRRYDLSGPRYTSYPTAVQFHEGIGPFDQLHALRDSRKAGHPLSLYVHIPFCANICYYCAC
NKVITKDRGRSAPYLARLVREIEIVSRHLSREQVVEQLHFGGGTPTFLSPGQLRELMSQLRTHLNLLDDDSG
DYGIEIDPREADWSTMGLLRELGFNRVSLGVQDFDMEVQKAVNRMQTPEETRTIVEAARTLQYRSINLDLIY
GLPKQTPDSFARTVDEVIALQPDRLSVFNYAHLPERFMPQRRINADDLPSPGQKLEMLQRTTEQLAAAGYRY
IGMDHFALPDDELASAQEDGTLQRNFQGYTTHGHCDLVGLGVSAISQIGDLYSQNSSDINDYQTSLDNGQLA
IRRGLHCNSDDRVRRAVIQQLICHFELAFEDIETEFGIDFRSYFAELWPDLERFAADGLIRLDAKGIDITSS
GRLLVRSICMLFDRYLPSLNRQRFSRVI*
AnnotationID:958GeneID:949
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 06:06:45
Gene Name:hemN
Confidence Code:1
GeneProduct:oxygen-independent coproporphyrinogen III
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0635
Secondary Functional Category(ies):coenzyme metabolism
EC Number:1.3.99.22
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69844.1|AE016623_6 (AE016790) oxygen-independent coproporphyrinogen III oxidase
           [Pseudomonas putida KT2440]
          Length = 460

 Score =  724 bits (1869), Expect = 0.0
 Identities = 352/460 (76%), Positives = 395/460 (85%)

>gb|AAO55511.1| (AE016862) oxygen-independent coproporphyrinogen III oxidase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 460

 Score =  691 bits (1782), Expect = 0.0
 Identities = 337/460 (73%), Positives = 380/460 (82%)

>gb|AAG59056.1|AE005617_5 (AE005617) O2-independent coproporphyrinogen III oxidase
           [Escherichia coli O157:H7 EDL933]
          Length = 459

 Score =  444 bits (1142), Expect = e-124
 Identities = 235/457 (51%), Positives = 302/457 (66%), Gaps = 6/457 (1%)






 
Structural Features:
COG0635, HemN, Coproporphyrinogen III oxidase and related Fe-S oxidoreductases 
[Coenzyme metabolism]
CD-Length = 416 residues,  98.6% aligned
Score =  430 bits (1107), Expect = 2e-121
Identities = 167/432 (38%), Positives = 229/432 (53%), Gaps = 22/432 (5%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1901




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19043




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37312




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:949_cdd.html


    		




 
URL:

















    ReferenceID:37929




  
Author/Investigator(s):
        Vollack KU, Xie J, Hartig E, Romling U, Zumft WG.





  
Title:
        Localization of denitrification genes on the chromosomal map of Pseudomonas aeruginosa.




  
PubMed:
        9493381


MedLine:
        




    
Source:
Microbiology. 1998 Feb;144 ( Pt 2):441-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9493381

















    ReferenceID:37930




  
Author/Investigator(s):
        Xu K, Elliott T.





  
Title:
        Cloning, DNA sequence, and complementation analysis of the Salmonella typhimurium hemN gene encoding a putative oxygen-independent coproporphyrinogen III oxidase.




  
PubMed:
        8195073


MedLine:
        




    
Source:
J Bacteriol. 1994 Jun;176(11):3196-203.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8195073









           
Homologs By Global Alignment 

           

       



     
Gene ID:949
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1680
                           PA1546_tr
                           translation of PA1546
                           460
                           99.99
                           99.99
                           0
                           2405.0
     

     
     
60027
                           gb|AAG04935.1|AE004582_13
                           (AE004582) oxygen-independent coproporphyrinogen III oxidase [Pseudomonas aeruginosa PAO1]
                           460
                           99.99
                           99.99
                           0
                           2405.0
     

     
     
60028
                           emb|CAA66617.1|
                           (X97981) oxygen-independent coproporphyrinogen III dehyrogenase [Pseudomonas aeruginosa]
                           460
                           99.56
                           99.56
                           0
                           2392.0
     

     
     
60029
                           gb|AAN69844.1|AE016623_6
                           (AE016790) oxygen-independent coproporphyrinogen III oxidase [Pseudomonas putida KT2440]
                           460
                           76.52
                           85.86
                           0
                           1869.0
     

     
     
60030
                           gb|AAK49511.1|AF321091_1
                           (AF321091) oxygen-independent coproporphyrinogen III dehydrogenase [Pseudomonas putida]
                           463
                           73.65
                           84.01
                           5
                           1803.0
     

     






      

          
GC ORFID: 43951How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1546Glimmer Score: 

          
Start: 994334Stop: 992952

          
Length: 1383


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1546 translation of PA1546Homolog Bit Score 931.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7536 (ORF: BLASTX 697.0), t_PA1546 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGACACCATTCGCTGGGACGCTGACCTGATTCGGCGCTACGACCTTTCCGGCCCGCGTTACACCTCC 
TATCCGACCGCCGTGCAGTTCCACGAAGGCATCGGCCCGTTCGACCAGTTGCACGCCCTGCGCGACAGCCGG 
AAGGCCGGACACCCCCTGTCGCTCTACGTGCACATCCCGTTCTGCGCCAACATCTGCTACTACTGCGCCTGC 
AACAAGGTGATCACCAAGGATCGCGGGCGCAGCGCGCCGTACCTCGCGCGCCTGGTCCGCGAGATCGAGATC 
GTCAGCCGCCACCTCTCCCGCGAGCAGGTGGTCGAGCAACTGCACTTCGGTGGCGGAACCCCGACCTTCCTC 
AGTCCCGGCCAGTTGCGCGAACTCATGTCGCAATTGCGCACCCACCTCAACCTGCTGGACGACGATTCGGGC 
GACTACGGCATCGAGATCGACCCACGCGAGGCCGACTGGTCGACCATGGGCCTGCTCCGCGAACTCGGTTTC 
AACCGCGTCAGCCTCGGCGTACAGGACTTCGACATGGAGGTGCAGAAGGCGGTCAACCGCATGCAGACCCCG 
GAGGAGACCCGTACCATCGTCGAGGCCGCGCGCACCCTGCAGTACCGCTCGATCAACCTCGACCTGATCTAC 
GGACTGCCGAAACAGACGCCGGACAGCTTCGCCCGCACGGTCGACGAAGTGATCGCGCTACAGCCGGATCGC 
CTCTCGGTGTTCAACTACGCCCACCTGCCCGAGCGTTTCATGCCGCAGCGGCGGATCAACGCCGACGACCTG 
CCGAGCCCGGGCCAGAAGCTGGAAATGCTCCAGCGCACCACCGAGCAACTCGCCGCGGCGGGCTACCGCTAT 
ATCGGCATGGATCACTTCGCCCTGCCCGACGACGAGCTGGCCAGCGCCCAGGAAGACGGCACCCTGCAACGC 
AATTTCCAGGGCTACACCACGCACGGGCACTGTGACCTGGTAGGTCTCGGGGTCTCGGCGATCAGCCAGATC 
GGCGACCTCTACAGCCAGAACAGCAGCGACATCAACGACTACCAGACCAGCCTCGACAACGGCCAGTTGGCG 
ATCCGTCGCGGCCTGCACTGCAACAGCGACGACCGGGTGCGGCGCGCGGTGATCCAGCAACTGATCTGCCAC 
TTTGAACTGGCCTTCGAGGATATCGAGACGGAGTTCGGCATCGACTTCCGCAGCTACTTCGCCGAGCTGTGG 
CCCGACCTCGAACGCTTCGCCGCCGACGGCCTGATCCGCCTGGACGCCAAGGGCATCGACATCACCTCCTCC 
GGGCGTCTGCTGGTGCGTTCGATCTGCATGCTGTTCGACCGCTACCTGCCCAGCCTCAACCGGCAGCGCTTC 
TCCCGGGTGATCTGA