ViewGene
Locus Name:
GeneID:
Location:

GeneID:943
TrivialName: PA14_05550
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05550
PAO1 Ortholog Locus:PA0427
Sequence Length:1458
Protein Length:485
Start: 491084
Stop: 492541
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03816.1|AE004479_11 (AE004479) Major intrinsic multiple antibiotic resistance efflux outer membrane protein OprM precursor [Pseudomonas aeruginosa PAO1]
Identities = 485/485 (100%), Positives = 485/485 (100%)
Sequence:
ATGAAACGGTCCTTCCTTTCCCTGGCGGTAGCCGCTGTCGTTCTGTCCGGCTGCTCGCTGATCCCCGACTAC
CAGCGCCCCGAGGCGCCGGTAGCCGCGGCCTACCCGCAAGGGCAGGCCTACGGGCAGAACACCGGCGCGGCG
GCCGTTCCGGCCGCCGACATCGGCTGGCGCGAGTTCTTCCGCGACCCGCAGTTGCAGCAACTGATCGGCGTG
GCGCTGGAAAACAACCGCGACCTGCGGGTCGCCGCGTTGAACGTCGAGGCCTTCCGGGCGCAGTACCGCATC
CAGCGGGCCGACCTGTTCCCGCGGATCGGCGTGGACGGTAGCGGCACCCGCCAGCGTTTGCCGGGCGACCTG
TCGACCACCGGCAGTCCGGCGATTTCCAGCCAGTACGGGGTGACCCTGGGCACTACCGCCTGGGAACTCGAT
CTCTTCGGCCGCCTGCGCAGCCTGCGCGACCAGGCCCTGGAGCAGTACCTGGCGACCGAACAGGCGCAGCGC
AGCGCGCAGACCACCCTGGTGGCCAGCGTGGCGACCGCCTACCTGACGCTGAAGGCCGACCAGGCGCAGTTG
CAACTGACCAAGGACACCCTGGGCACCTACCAGAAGAGTTTCGACCTGACCCAGCGCAGCTACGACGTCGGC
GTCGCCTCCGCGCTCGACCTGCGCCAGGCGCAGACCGCCGTGGAAGGCGCCCGCGCGACCCTGGCGCAGTAC
ACCCGCCTGGTAGCCCAGGACCAGAATGCACTGGTCCTGCTGCTGGGTTCCGGGATCCCGGCGAACCTGCCG
CAAGGCCTGGGCCTGGACCAGACCCTGCTGACCGAAGTGCCGGCGGGCCTGCCGTCGGACCTGCTGCAACGG
CGCCCGGACATCCTCGAGGCCGAGCACCAGCTCATGGCCGCCAACGCCAGCATCGGCGCCGCGCGCGCGGCG
TTCTTCCCGAGCATCAGCCTGACCGCCAACGCCGGCACCATGAGCCGCCAGCTGTCCGGCCTGTTCGACGCC
GGTTCGGGCTCCTGGTTGTTCCAGCCGTCGATCAACCTGCCGATCTTCACCGCCGGCAGCCTGCGCGCCAGC
CTGGACTACGCGAAGATCCAGAAGGACATCAACGTCGCGCAGTACGAGAAGGCGATCCAGACCGCCTTCCAG
GAAGTCGCCGACGGTCTGGCCGCGCGCGGTACCTTCACCGAGCAGTTGCAGGCACAGCGCGATCTGGTCAAG
GCCAGCGACGAGTACTACCAGCTCGCCGACAAGCGCTACCGCACGGGGGTGGACAACTACCTGACCCTGCTC
GACGCGCAACGCTCGCTGTTCACCGCGCAGCAGCAACTGATCACCGACCGCCTCAACCAGCTGACCAGCGAG
GTCAACCTGTACAAGGCCCTCGGCGGCGGCTGGAACCAGCAGACCGTGACCCAGCAGCAGACCGCGAAGAAG
GAAGATCCGCAGGCCTGA
Translation:
MKRSFLSLAVAAVVLSGCSLIPDYQRPEAPVAAAYPQGQAYGQNTGAAAVPAADIGWREFFRDPQLQQLIGV
ALENNRDLRVAALNVEAFRAQYRIQRADLFPRIGVDGSGTRQRLPGDLSTTGSPAISSQYGVTLGTTAWELD
LFGRLRSLRDQALEQYLATEQAQRSAQTTLVASVATAYLTLKADQAQLQLTKDTLGTYQKSFDLTQRSYDVG
VASALDLRQAQTAVEGARATLAQYTRLVAQDQNALVLLLGSGIPANLPQGLGLDQTLLTEVPAGLPSDLLQR
RPDILEAEHQLMAANASIGAARAAFFPSISLTANAGTMSRQLSGLFDAGSGSWLFQPSINLPIFTAGSLRAS
LDYAKIQKDINVAQYEKAIQTAFQEVADGLAARGTFTEQLQAQRDLVKASDEYYQLADKRYRTGVDNYLTLL
DAQRSLFTAQQQLITDRLNQLTSEVNLYKALGGGWNQQTVTQQQTAKKEDPQA*
AnnotationID:952GeneID:943
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 09:09:56
Gene Name:oprM
Confidence Code:1
GeneProduct:major intrinsic multiple antibiotic resistance efflux outer membrane protein OprM precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1538,pfam02321
Secondary Functional Category(ies):antibiotic resistance and susceptibility
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57756.1| (AE016871) outer membrane efflux protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 486

 Score =  646 bits (1667), Expect = 0.0
 Identities = 333/483 (68%), Positives = 400/483 (82%), Gaps = 2/483 (0%)

>gb|AAF73833.1|AF183959_4 (AF183959) outer membrane channel protein [Pseudomonas putida]
          Length = 484

 Score =  642 bits (1656), Expect = 0.0
 Identities = 332/484 (68%), Positives = 396/484 (81%), Gaps = 1/484 (0%)








 
Structural Features:
COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / Intracellular trafficking and secretion]
CD-Length = 457 residues,  99.6% aligned
Score =  264 bits (675), Expect = 2e-71
Identities = 209/466 (44%), Positives = 268/466 (57%), Gaps = 12/466 (2%)

pfam02321, OEP, Outer membrane efflux protein. 
CD-Length = 188 residues, 100.0% aligned
Score =  116 bits (292), Expect = 5e-27
Identities = 66/188 (35%), Positives = 100/188 (53%), Gaps = 1/188 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1889




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19031




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33752




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:943_cdd.html


    		




 
URL:

















    ReferenceID:35359




  
Author/Investigator(s):
        Gotoh N, Tsujimoto H, Poole K, Yamagishi J, Nishino T.





  
Title:
        The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon.




  
PubMed:
        8585747


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1995 Nov;39(11):2567-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8585747

















    ReferenceID:35360




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173









           
Homologs By Global Alignment 

           

       



     
Gene ID:943
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1670
                           PA0427_tr
                           translation of PA0427
                           485
                           99.99
                           99.99
                           0
                           2415.0
     

     
     
59772
                           gb|AAG03816.1|AE004479_11
                           (AE004479) Major intrinsic multiple antibiotic resistance efflux outer membrane protein OprM precursor [Pseudomonas aeruginosa PAO1]
                           485
                           99.99
                           99.99
                           0
                           2415.0
     

     
     
59773
                           dbj|BAA28694.1|
                           (AB011381) OprM [Pseudomonas aeruginosa]
                           485
                           99.99
                           99.99
                           0
                           2415.0
     

     
     
59774
                           gb|AAA74435.1|
                           (L23839) outer membrane protein [Pseudomonas aeruginosa]
                           485
                           96.08
                           96.49
                           8
                           2297.0
     

     
     
59775
                           gb|AAQ92182.1|
                           (AY349612) outer membrane protein [Pseudomonas fluorescens]
                           488
                           69.05
                           82.17
                           5
                           1682.0
     

     






      

          
GC ORFID: 43945How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0427Glimmer Score: 

          
Start: 6292426Stop: 6293883

          
Length: 1458


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0427 translation of PA0427Homolog Bit Score 934.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6179 (ORF: BLASTX 652.0), psyr_15may02_Scaffold1_revised_gene30 (ORF: BLASTX 592.0), psyr_15may02_Scaffold7_revised_gene7338 (ORF: BLASTX 457.0), t_PA4597 (ORF: BLASTX 384.0), t_PA0427 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACGGTCCTTCCTTTCCCTGGCGGTAGCCGCTGTCGTTCTGTCCGGCTGCTCGCTGATCCCCGACTAC 
CAGCGCCCCGAGGCGCCGGTAGCCGCGGCCTACCCGCAAGGGCAGGCCTACGGGCAGAACACCGGCGCGGCG 
GCCGTTCCGGCCGCCGACATCGGCTGGCGCGAGTTCTTCCGCGACCCGCAGTTGCAGCAACTGATCGGCGTG 
GCGCTGGAAAACAACCGCGACCTGCGGGTCGCCGCGTTGAACGTCGAGGCCTTCCGGGCGCAGTACCGCATC 
CAGCGGGCCGACCTGTTCCCGCGGATCGGCGTGGACGGTAGCGGCACCCGCCAGCGTTTGCCGGGCGACCTG 
TCGACCACCGGCAGTCCGGCGATTTCCAGCCAGTACGGGGTGACCCTGGGCACTACCGCCTGGGAACTCGAT 
CTCTTCGGCCGCCTGCGCAGCCTGCGCGACCAGGCCCTGGAGCAGTACCTGGCGACCGAACAGGCGCAGCGC 
AGCGCGCAGACCACCCTGGTGGCCAGCGTGGCGACCGCCTACCTGACGCTGAAGGCCGACCAGGCGCAGTTG 
CAACTGACCAAGGACACCCTGGGCACCTACCAGAAGAGTTTCGACCTGACCCAGCGCAGCTACGACGTCGGC 
GTCGCCTCCGCGCTCGACCTGCGCCAGGCGCAGACCGCCGTGGAAGGCGCCCGCGCGACCCTGGCGCAGTAC 
ACCCGCCTGGTAGCCCAGGACCAGAATGCACTGGTCCTGCTGCTGGGTTCCGGGATCCCGGCGAACCTGCCG 
CAAGGCCTGGGCCTGGACCAGACCCTGCTGACCGAAGTGCCGGCGGGCCTGCCGTCGGACCTGCTGCAACGG 
CGCCCGGACATCCTCGAGGCCGAGCACCAGCTCATGGCCGCCAACGCCAGCATCGGCGCCGCGCGCGCGGCG 
TTCTTCCCGAGCATCAGCCTGACCGCCAACGCCGGCACCATGAGCCGCCAGCTGTCCGGCCTGTTCGACGCC 
GGTTCGGGCTCCTGGTTGTTCCAGCCGTCGATCAACCTGCCGATCTTCACCGCCGGCAGCCTGCGCGCCAGC 
CTGGACTACGCGAAGATCCAGAAGGACATCAACGTCGCGCAGTACGAGAAGGCGATCCAGACCGCCTTCCAG 
GAAGTCGCCGACGGTCTGGCCGCGCGCGGTACCTTCACCGAGCAGTTGCAGGCACAGCGCGATCTGGTCAAG 
GCCAGCGACGAGTACTACCAGCTCGCCGACAAGCGCTACCGCACGGGGGTGGACAACTACCTGACCCTGCTC 
GACGCGCAACGCTCGCTGTTCACCGCGCAGCAGCAACTGATCACCGACCGCCTCAACCAGCTGACCAGCGAG 
GTCAACCTGTACAAGGCCCTCGGCGGCGGCTGGAACCAGCAGACCGTGACCCAGCAGCAGACCGCGAAGAAG 
GAAGATCCGCAGGCCTGA