ViewGene
Locus Name:
GeneID:
Location:

GeneID:939
TrivialName: PA14_32080
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32080
PAO1 Ortholog Locus:PA2518
Sequence Length:1368
Protein Length:455
Start: 2791292
Stop: 2792659
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05906.1|AE004678_12 (AE004678) toluate 1,2-dioxygenase alpha subunit [Pseudomonas aeruginosa PAO1]
Identities = 453/455 (99%), Positives = 454/455 (99%)
Sequence:
ATGTCCCTGGGTAGCGATTACTTGCGTTCTCTGCTCGAGGAAGACCCCGAGCGCGGCGTCTACCGCTGCAAG
CGGGAGATGTTCACCGACCCACGCCTGTTCGAGCTGGAGATGAAGCACATCTTCGAGGGCAACTGGGTCTAT
CTCGCCCACGAAAGCCAGGTTGCCGGAATCAACGACTACCTGACCACCCAGATCGGCCGCCAGTCGATCGTC
ATCGCGCGCAACCGCGACGGCCAGCTCAACGCCTTCATCAACGCCTGCAGTCACCGTGGCGCGATGCTCTGC
CGGCACAAGAGCGGCAATCGCAGCAGCTATACCTGTCCGTTCCACGGCTGGACCTTCAACAACTCCGGCAAG
CTGCTCAAGGTCAAGGACCCGGCCGAGGCCGGCTACCCGCAGGGCTTCAACTGCGAGGGCTCGCACGACCTG
ACCCGGGTGGCGCGCTTCGAGTCCTACCGTGGCTTCCTGTTCGGCAGCCTCAACCCCGATGTCAGGCCGCTG
GCCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGACCAGTCGCCGGAAGGCCTCGAAGTG
CTGCGCGGCTCTTCCAGCTATGTCTACGAAGGCAACTGGAAGCTTACCGCCGAGAACGGCGCCGACGGCTAC
CACGTCAGTTCGGTGCACTGGAACTACGCGGCGACCCAGAGCCAGCGCCAGCAGCGCGACGCCGCGGACCag
CTGCGGACCATGAGCGCCGCCGGCTGGGCCCGGCAGGGCGGCGGCTTCTACTCGTTCGAGCATGGCCACATG
CTGCTCTGGAGTCGCTGGGCCAACCCCGAGgACCGGCCGGCCTTCGAGCGCCGCGCCGAGCTTGCCCGCGAC
TTCGgCGAGGCCCGCGCCGACTGGATGATCGAGAACTCGCGCAACCTCTGCCTGTACCCCAACGTCTACCTG
ATGGACCAGTTCAGCTCGCAGATCCGCATCGCCCGGCCGCTGTCGGTGGACCGCACGGAAATCACCATCTAC
TGCATCGCGCCGAAAGGCGAGAGCGCCGAGGCGCGCGCCCGGCGCATCCGCCAGTACGAGGACTTCTTCAAC
GTCAGCGGCATGGCCACCCCGGACGACCTGGAGGAGTTCCGTTCCTGCCAGCAGGGCTACCAGGGCAGCGTG
GTCGGCTGGAACGACCTGTCGCGCGGCGCCGAACACTGGATAGAGGGCGCCGACGCGGCGGCCGCGGCCATC
GACCTGCGGCCGCGCCTGAGCGGCGTGCGCACCGAGGACGAAGGGCTGTTCGTATTGCAGCATCGCTACTGG
CAGGAGGCGCTGCTCGCCGCCCTGGAGCGCGAACGGACGCAGCGCGTGGCGCTGCGGGAGGTGGCGTCATGA
Translation:
MSLGSDYLRSLLEEDPERGVYRCKREMFTDPRLFELEMKHIFEGNWVYLAHESQVAGINDYLTTQIGRQSIV
IARNRDGQLNAFINACSHRGAMLCRHKSGNRSSYTCPFHGWTFNNSGKLLKVKDPAEAGYPQGFNCEGSHDL
TRVARFESYRGFLFGSLNPDVRPLAEHLGESAKIIDMIVDQSPEGLEVLRGSSSYVYEGNWKLTAENGADGY
HVSSVHWNYAATQSQRQQRDAADQLRTMSAAGWARQGGGFYSFEHGHMLLWSRWANPEDRPAFERRAELARD
FGEARADWMIENSRNLCLYPNVYLMDQFSSQIRIARPLSVDRTEITIYCIAPKGESAEARARRIRQYEDFFN
VSGMATPDDLEEFRSCQQGYQGSVVGWNDLSRGAEHWIEGADAAAAAIDLRPRLSGVRTEDEGLFVLQHRYW
QEALLAALERERTQRVALREVAS*
AnnotationID:948GeneID:939
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 09:09:17
Gene Name:xylX
Confidence Code:2
GeneProduct:toluate 1,2-dioxygenase alpha subunit
Cell Localization:()
Synonyms:benA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:benzoate dioxygenase, alpha subunit
Functional Category Confidence Code:5
COGs:COG4638
Secondary Functional Category(ies):transport of small molecules
EC Number:1.14.12.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK52287.1| (AY026914) benzoate 1,2-dioxygenase large subunit BenA [Pseudomonas
           putida]
          Length = 455

 Score =  932 bits (2409), Expect = 0.0
 Identities = 452/455 (99%), Positives = 454/455 (99%)

>emb|CAD92319.1| (AJ564846) benzoate 1,2 dioxygenase alpha subunit [Acinetobacter
           calcoaceticus]
          Length = 460

 Score =  657 bits (1694), Expect = 0.0
 Identities = 304/439 (69%), Positives = 357/439 (81%)

>dbj|BAC19115.1| (AP005221) putative benzoate 1,2-dioxygenase reductase
           [Corynebacterium efficiens YS-314]
          Length = 498

 Score =  605 bits (1560), Expect = e-172
 Identities = 277/436 (63%), Positives = 340/436 (77%), Gaps = 1/436 (0%)








 
Structural Features:
COG4638, HcaE, Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, 
large terminal subunit 
[Inorganic ion transport and metabolism]
CD-Length = 367 residues,  99.2% aligned
Score =  215 bits (548), Expect = 1e-56
Identities = 99/428 (23%), Positives = 150/428 (35%), Gaps = 65/428 (15%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1881




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19023




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33075




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:939_cdd.html


    		




 
URL:

















    ReferenceID:34516




  
Author/Investigator(s):
        Harayama S, Rekik M, Bairoch A, Neidle EL, Ornston LN.





  
Title:
        Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate dioxygenases.




  
PubMed:
        1938949


MedLine:
        




    
Source:
J Bacteriol. 1991 Dec;173(23):7540-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1938949

















    ReferenceID:34517




  
Author/Investigator(s):
        Harayama S, Rekik M, Timmis KN.





  
Title:
        Genetic analysis of a relaxed substrate specificity aromatic ring dioxygenase, toluate 1,2-dioxygenase, encoded by TOL plasmid pWW0 of Pseudomonas putida.




  
PubMed:
        3010045


MedLine:
        




    
Source:
Mol Gen Genet. 1986 Feb;202(2):226-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3010045

















    ReferenceID:34518




  
Author/Investigator(s):
        Park HJ, Kim ES.





  
Title:
        An inducible Streptomyces gene cluster involved in aromatic compound metabolism.




  
PubMed:
        13129621


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2003 Sep 12;226(1):151-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=13129621









           
Homologs By Global Alignment 

           

       



     
Gene ID:939
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1659
                           PA2518_tr
                           translation of PA2518
                           455
                           99.56
                           99.78
                           0
                           2414.0
     

     
     
59373
                           gb|AAG05906.1|AE004678_12
                           (AE004678) toluate 1,2-dioxygenase alpha subunit [Pseudomonas aeruginosa PAO1]
                           455
                           99.56
                           99.78
                           0
                           2414.0
     

     
     
59374
                           gb|AAK52287.1|
                           (AY026914) benzoate 1,2-dioxygenase large subunit BenA [Pseudomonas putida]
                           455
                           99.34
                           99.78
                           0
                           2409.0
     

     
     
59375
                           gb|AAF63448.2|AF218267_5
                           (AF218267) benzoate dioxygenase - alpha subunit [Pseudomonas putida]
                           455
                           80.00
                           90.98
                           3
                           2038.0
     

     
     
59376
                           gb|AAN68769.1|AE016509_2
                           (AE016785) benzoate dioxygenase, alpha subunit [Pseudomonas putida KT2440]
                           455
                           80.00
                           90.98
                           3
                           2037.0
     

     






      

          
GC ORFID: 43941How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2518Glimmer Score: 

          
Start: 2162632Stop: 2161265

          
Length: 1368


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2518 translation of PA2518Homolog Bit Score 934.0

          
Other Homologs:  t_PA2512 (ORF: BLASTX 428.0), t_PA2518 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCTGGGTAGCGATTACTTGCGTTCTCTGCTCGAGGAAGACCCCGAGCGCGGCGTCTACCGCTGCAAG 
CGGGAGATGTTCACCGACCCACGCCTGTTCGAGCTGGAGATGAAGCACATCTTCGAGGGCAACTGGGTCTAT 
CTCGCCCACGAAAGCCAGGTTGCCGGAATCAACGACTACCTGACCACCCAGATCGGCCGCCAGTCGATCGTC 
ATCGCGCGCAACCGCGACGGCCAGCTCAACGCCTTCATCAACGCCTGCAGTCACCGTGGCGCGATGCTCTGC 
CGGCACAAGAGCGGCAATCGCAGCAGCTATACCTGTCCGTTCCACGGCTGGACCTTCAACAACTCCGGCAAG 
CTGCTCAAGGTCAAGGACCCGGCCGAGGCCGGCTACCCGCAGGGCTTCAACTGCGAGGGCTCGCACGACCTG 
ACCCGGGTGGCGCGCTTCGAGTCCTACCGTGGCTTCCTGTTCGGCAGCCTCAACCCCGATGTCAGGCCGCTG 
GCCGAGCACCTGGGCGAGTCGGCGAAGATCATCGACATGATCGTCGACCAGTCGCCGGAAGGCCTCGAAGTG 
CTGCGCGGCTCTTCCAGCTATGTCTACGAAGGCAACTGGAAGCTTACCGCCGAGAACGGCGCCGACGGCTAC 
CACGTCAGTTCGGTGCACTGGAACTACGCGGCGACCCAGAGCCAGCGCCAGCAGCGCGACGCCGCGGACCag 
CTGCGGACCATGAGCGCCGCCGGCTGGGCCCGGCAGGGCGGCGGCTTCTACTCGTTCGAGCATGGCCACATG 
CTGCTCTGGAGTCGCTGGGCCAACCCCGAGgACCGGCCGGCCTTCGAGCGCCGCGCCGAGCTTGCCCGCGAC 
TTCGgCGAGGCCCGCGCCGACTGGATGATCGAGAACTCGCGCAACCTCTGCCTGTACCCCAACGTCTACCTG 
ATGGACCAGTTCAGCTCGCAGATCCGCATCGCCCGGCCGCTGTCGGTGGACCGCACGGAAATCACCATCTAC 
TGCATCGCGCCGAAAGGCGAGAGCGCCGAGGCGCGCGCCCGGCGCATCCGCCAGTACGAGGACTTCTTCAAC 
GTCAGCGGCATGGCCACCCCGGACGACCTGGAGGAGTTCCGTTCCTGCCAGCAGGGCTACCAGGGCAGCGTG 
GTCGGCTGGAACGACCTGTCGCGCGGCGCCGAACACTGGATAGAGGGCGCCGACGCGGCGGCCGCGGCCATC 
GACCTGCGGCCGCGCCTGAGCGGCGTGCGCACCGAGGACGAAGGGCTGTTCGTATTGCAGCATCGCTACTGG 
CAGGAGGCGCTGCTCGCCGCCCTGGAGCGCGAACGGACGCAGCGCGTGGCGCTGCGGGAGGTGGCGTCATGA