ViewGene
Locus Name:
GeneID:
Location:

GeneID:938
TrivialName: PA14_35460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35460
PAO1 Ortholog Locus:PA2252
Sequence Length:1446
Protein Length:481
Start: 3153542
Stop: 3152097
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05640.1|AE004651_2 (AE004651) probable AGCS sodium/alanine/glycine symporter [Pseudomonas aeruginosa PAO1]
Identities = 479/481 (99%), Positives = 479/481 (99%)
Sequence:
ATGCTTGAAATGCTCAACGATTTCCTTTCCGGAAAAGTCCTCATCGTCCTGATCGTCGGCCTGGGCGCCTAC
TTCACGATCCGTTCGCGCTTCGTCCAGTTCCGCCACTTCGGCCACATGTTCGGCGTGTTCAGGGAATCACTG
CGCGGCCAGGCCGGCCAGCTCAGTTCCTTCCAGGCCCTGATGCTGAGCCTCGCCGGCCGGGTCGGCGCCGGC
AACATCGCCGGGGTCGGCATCGCCGTGACCCTCGGCGGTCCGGGGGCGGTGTTCTGGATGTGGGTCACCGCG
CTGGTCGGCATGTCCAGCAGCTTCTTCGAATGCACCCTGGCCCAGGTCTACAAGCGCAGCGACGGCGACGGC
CTGTATCGCGGCGGCCCGTCCTACTACATCCAGCACGGCCTGAAGCTGCGCTCGATGGCGGTGGTCTTCGCG
GTACTCCTGCTGGTGACCTACGGCTTCGCCTTCAACGGCCTGCAGTCCTACACCGTCACCCATTCGCTGCAG
AACGCCTTCGGCTTCGCCCCGCAGCACACCGGGATCGCCCTGGCGGTGCTGCTCGGCGTGGTCTTCATCGGC
GGTATCAAGCGCATCGCCGCGGTCTCCGACCTGCTGGTGCCGATCAAGACCCTGGCCTACATCGGTGTCACC
CTGTACGTCATCGCCAGCCAGATCGAGCACGTCCCGGCAATGCTGGAAACCATCGTCAAGAGCGCCTTCGGC
CTCGACCCGGCGTTCGGCGGCCTACTCGGCAGCGCCATCGTGATGGGCGTCAAGCGCGGCGTCTTCGCCAAC
GAGGCCGGCCTGGGCAGCGCGCCGAACGTCGCCGCGGTCGCCGCCGTGCGCCATCCGGCGGCGCAGGGCGTG
GTCCAGGCCTTCAGCGTGTTCCTCGACACCTTCGTGATCTGCACCTGCACCGCCCTGCTGATCCTGCTCTCG
GGCTTCTACACCCCGGGCTTCGAAGGCGACGGCATCGTCCTCACCCAGAACTCCCTGGCCGCGGTGGTCGGC
GACTGGGGCCGGGTGTTCGTCAGCGTGGCCCTGTCGCTGTTCGTCTTCACCTGCATCCTCTACAACTTCTAC
CTGGGCGAGAACAGCCTGCAGTTCCTCAGCCGCAACCGCCTGGTGCTGATCGGCTACCGCGCGCTGGTGCTG
GCCCTGGTGGTCTGGGGCTCGGTGCAGGACCTGTCCACCGTGTTCGCCTTCGCCGACATCACCATGACCTGC
CTGGCCTTCGTCAACCTGATGGCCCTGGCTCTGCTGTTCAAGGTCGGCCTGCGGGTGATGCGCGACTACGAC
GTGCAGCGCCGCGCCGGGATCAAGCAGCCGGTGTTCGACTCGGCGCAGTTCGCCGACCTCGACCTGGACCGC
GAAGCCTGGCCCGCCGGCCAGCACGCCGAGCCCCCGGCGCAGGCGGACGCCGAGCCGCTGCCGAGCGGCACT
CGCTGA
Translation:
MLEMLNDFLSGKVLIVLIVGLGAYFTIRSRFVQFRHFGHMFGVFRESLRGQAGQLSSFQALMLSLAGRVGAG
NIAGVGIAVTLGGPGAVFWMWVTALVGMSSSFFECTLAQVYKRSDGDGLYRGGPSYYIQHGLKLRSMAVVFA
VLLLVTYGFAFNGLQSYTVTHSLQNAFGFAPQHTGIALAVLLGVVFIGGIKRIAAVSDLLVPIKTLAYIGVT
LYVIASQIEHVPAMLETIVKSAFGLDPAFGGLLGSAIVMGVKRGVFANEAGLGSAPNVAAVAAVRHPAAQGV
VQAFSVFLDTFVICTCTALLILLSGFYTPGFEGDGIVLTQNSLAAVVGDWGRVFVSVALSLFVFTCILYNFY
LGENSLQFLSRNRLVLIGYRALVLALVVWGSVQDLSTVFAFADITMTCLAFVNLMALALLFKVGLRVMRDYD
VQRRAGIKQPVFDSAQFADLDLDREAWPAGQHAEPPAQADAEPLPSGTR*
AnnotationID:947GeneID:938
AnnotatorUID: diggins
Modification Date/Time: 2006-02-05 16:04:56
Gene Name:
Confidence Code:3
GeneProduct:putative sodium/alanine symporter
Cell Localization:()
Synonyms:PA2252
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1115,pfam01235
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66125.1|AE016241_2 (AE016775) sodium/alanine symporter [Pseudomonas putida KT2440]
          Length = 480

 Score =  833 bits (2152), Expect = 0.0
 Identities = 419/466 (89%), Positives = 444/466 (95%)

>gb|AAO58919.1| (AE016876) sodium/alanine transporter [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 483

 Score =  714 bits (1842), Expect = 0.0
 Identities = 363/479 (75%), Positives = 408/479 (85%), Gaps = 1/479 (0%)

>dbj|BAB33430.1| (AP002550) putative inner membrane transport protein [Escherichia
           coli O157:H7]
          Length = 476

 Score =  366 bits (940), Expect = e-100
 Identities = 201/464 (43%), Positives = 285/464 (61%), Gaps = 10/464 (2%)






 
Structural Features:
COG1115, AlsT, Na+/alanine symporter [Amino acid transport and metabolism].
CD-Length = 452 residues,  98.2% aligned
Score =  501 bits (1292), Expect = 6e-143
Identities = 225/444 (50%), Positives = 291/444 (65%), Gaps = 6/444 (1%)

pfam01235, Na_Ala_symp, Sodium:alanine symporter family.
CD-Length = 415 residues, 100.0% aligned
Score =  406 bits (1046), Expect = 2e-114
Identities = 205/419 (48%), Positives = 282/419 (67%), Gaps = 10/419 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1879




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17018




  
Author/Investigator(s):
        





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:
NCBI - Conserved Domain Search (BLASTP).






    
Reference Type:Blast (text/plain)







    
Data:PA2252_CDS.cgi


    		




 
URL:

















    ReferenceID:19021




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:938
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1657
                           PA2252_tr
                           translation of PA2252
                           481
                           99.58
                           99.58
                           0
                           2415.0
     

     
     
59311
                           gb|AAG05640.1|AE004651_2
                           (AE004651) probable AGCS sodium/alanine/glycine symporter [Pseudomonas aeruginosa PAO1]
                           481
                           99.58
                           99.58
                           0
                           2415.0
     

     
     
59312
                           gb|AAN66125.1|AE016241_2
                           (AE016775) sodium/alanine symporter [Pseudomonas putida KT2440]
                           486
                           86.41
                           91.76
                           11
                           2143.0
     

     
     
59313
                           gb|AAO58919.1|
                           (AE016876) sodium/alanine transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           488
                           75.40
                           84.22
                           12
                           1845.0
     

     
     
59314
                           gb|AAO09054.1|AE016798_214
                           (AE016798) Na+/alanine symporter [Vibrio vulnificus CMCP6]
                           493
                           45.23
                           65.31
                           29
                           1137.0
     

     






      

          
GC ORFID: 43940How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2252Glimmer Score: 

          
Start: 1800374Stop: 1801819

          
Length: 1446


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2252 translation of PA2252Homolog Bit Score 934.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2910 (ORF: BLASTX 714.0), t_PA2252 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTTGAAATGCTCAACGATTTCCTTTCCGGAAAAGTCCTCATCGTCCTGATCGTCGGCCTGGGCGCCTAC 
TTCACGATCCGTTCGCGCTTCGTCCAGTTCCGCCACTTCGGCCACATGTTCGGCGTGTTCAGGGAATCACTG 
CGCGGCCAGGCCGGCCAGCTCAGTTCCTTCCAGGCCCTGATGCTGAGCCTCGCCGGCCGGGTCGGCGCCGGC 
AACATCGCCGGGGTCGGCATCGCCGTGACCCTCGGCGGTCCGGGGGCGGTGTTCTGGATGTGGGTCACCGCG 
CTGGTCGGCATGTCCAGCAGCTTCTTCGAATGCACCCTGGCCCAGGTCTACAAGCGCAGCGACGGCGACGGC 
CTGTATCGCGGCGGCCCGTCCTACTACATCCAGCACGGCCTGAAGCTGCGCTCGATGGCGGTGGTCTTCGCG 
GTACTCCTGCTGGTGACCTACGGCTTCGCCTTCAACGGCCTGCAGTCCTACACCGTCACCCATTCGCTGCAG 
AACGCCTTCGGCTTCGCCCCGCAGCACACCGGGATCGCCCTGGCGGTGCTGCTCGGCGTGGTCTTCATCGGC 
GGTATCAAGCGCATCGCCGCGGTCTCCGACCTGCTGGTGCCGATCAAGACCCTGGCCTACATCGGTGTCACC 
CTGTACGTCATCGCCAGCCAGATCGAGCACGTCCCGGCAATGCTGGAAACCATCGTCAAGAGCGCCTTCGGC 
CTCGACCCGGCGTTCGGCGGCCTACTCGGCAGCGCCATCGTGATGGGCGTCAAGCGCGGCGTCTTCGCCAAC 
GAGGCCGGCCTGGGCAGCGCGCCGAACGTCGCCGCGGTCGCCGCCGTGCGCCATCCGGCGGCGCAGGGCGTG 
GTCCAGGCCTTCAGCGTGTTCCTCGACACCTTCGTGATCTGCACCTGCACCGCCCTGCTGATCCTGCTCTCG 
GGCTTCTACACCCCGGGCTTCGAAGGCGACGGCATCGTCCTCACCCAGAACTCCCTGGCCGCGGTGGTCGGC 
GACTGGGGCCGGGTGTTCGTCAGCGTGGCCCTGTCGCTGTTCGTCTTCACCTGCATCCTCTACAACTTCTAC 
CTGGGCGAGAACAGCCTGCAGTTCCTCAGCCGCAACCGCCTGGTGCTGATCGGCTACCGCGCGCTGGTGCTG 
GCCCTGGTGGTCTGGGGCTCGGTGCAGGACCTGTCCACCGTGTTCGCCTTCGCCGACATCACCATGACCTGC 
CTGGCCTTCGTCAACCTGATGGCCCTGGCTCTGCTGTTCAAGGTCGGCCTGCGGGTGATGCGCGACTACGAC 
GTGCAGCGCCGCGCCGGGATCAAGCAGCCGGTGTTCGACTCGGCGCAGTTCGCCGACCTCGACCTGGACCGC 
GAAGCCTGGCCCGCCGGCCAGCACGCCGAGCCCCCGGCGCAGGCGGACGCCGAGCCGCTGCCGAGCGGCACT 
CGCTGA