ViewGene
Locus Name:
GeneID:
Location:

GeneID:937
TrivialName: PA14_41360
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41360
PAO1 Ortholog Locus:PA1795
Sequence Length:1383
Protein Length:460
Start: 3689929
Stop: 3688547
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05184.1|AE004605_6 (AE004605) cysteinyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 459/460 (99%), Positives = 459/460 (99%)
Sequence:
GTGCTGCAGATCTACAACACGCTGAGCAAGACCAAAGAAGTCTTCACGCCGCTGGTCGGCAACCAGGTGCGC
ATGTACGTGTGCGGCATGACCGTGTACGACTACTGCCATCTGGGCCACGGCCGCAGCATGGTGGCGTTCGAC
GTGATCACCCGCTGGTTGCGCCACCGTGGCTACGACTTGACGTACGTGCGCAACATCACCGATATCGATGAC
AAGATCATCAATCGCGCCAACGAGAACGACGAACCGTTCGATGTGCTGACCGAGCGCATGATCGCGGCGATG
CACGAGGACGAGGCCCGGCTGAACATCCTCAAGCCGGACCAGGAGCCGCGCGCCACCGATCACATCGCCGGC
ATGCACGCGATGATCCAGACCCTGATCGACAAGGGCTATGCCTACGCGCCGGGCAACGGTGACGTGTACTAC
CGGGTCGGCAAGTTCGCTGGCTACGGCAAGTTGTCGCGGCGGCGGGTCGAGGACCTGCGCATCGGTGCGCGG
ATCGAACCGGGCGAAGCGAAGGAGGATCCGCTCGATTTCGTGCTCTGGAAGGGCGCCAAGCCGGGCGAGCCG
AGCTGGTCGTCGCCCTGGGGCGAGGGGCGCCCGGGCTGGCACATCGAATGCTCGGTGATGTCCACCTGCTGC
CTGGGCGATTCCTTCGACATCCATGGCGGTGGCAACGACCTGGAGTTCCCTCACCACGAGAACGAGATCGCC
CAGAGCGAGGCCGCGACTGGCAAGCCCTACGCCAAGTCCTGGCTGCACTGCGGGATGATTACCATCAACGGC
GAGAAGATGTCCAAGTCGCTGGGCAATTTCTTCACCATCCGCGAAGTGCTGGAGAAATATCACCCCGAAGTG
GTGCGTTACCTGCTGATCGCCAGCCATTACCGCAGTCCGATCAACTACTCGGAGGAGAACCTGCGCGAGGCC
AAGGCGGCCCTGGACCGTTTCTACAACGCGCTCAAGGGGTTGCCCGAGGCGGCGCCGGCGGAAGCGGCGGAG
TATGTCGAGCGCTTCGCCGCGGCGATGGACGACGACTTCAATACCGCGGGTGCCTGCTCGGTGCTGTTCGAG
CTGGCGCGGGAGGTCAATCGTCTGCGCGAAAGCGACCTGTCGGCGGCGGCCGCGCTGGCCGCGCGCCTGAAG
CAGTTGGCTGGCCTGCTGGGGGTGCTGCAGCTGGAGCCGGAAGCCTTCCTGCAGGCGGGCGCCGAGGGCAAG
GTCGATGCGGCCGAGGTCGAGGCGTTGATCCAGGCGCGCCTGGAGGCGCGGGCGGCGAAGAACTGGGCGGAG
TCCGACCGGATTCGCGACCAGCTCACCGCCATGGGCGTGGTGCTGGAGGATGGCAAGGGCGGCACTACCTGG
CGCCTGGCCGACTGA
Translation:
MLQIYNTLSKTKEVFTPLVGNQVRMYVCGMTVYDYCHLGHGRSMVAFDVITRWLRHRGYDLTYVRNITDIDD
KIINRANENDEPFDVLTERMIAAMHEDEARLNILKPDQEPRATDHIAGMHAMIQTLIDKGYAYAPGNGDVYY
RVGKFAGYGKLSRRRVEDLRIGARIEPGEAKEDPLDFVLWKGAKPGEPSWSSPWGEGRPGWHIECSVMSTCC
LGDSFDIHGGGNDLEFPHHENEIAQSEAATGKPYAKSWLHCGMITINGEKMSKSLGNFFTIREVLEKYHPEV
VRYLLIASHYRSPINYSEENLREAKAALDRFYNALKGLPEAAPAEAAEYVERFAAAMDDDFNTAGACSVLFE
LAREVNRLRESDLSAAAALAARLKQLAGLLGVLQLEPEAFLQAGAEGKVDAAEVEALIQARLEARAAKNWAE
SDRIRDQLTAMGVVLEDGKGGTTWRLAD*
AnnotationID:946GeneID:937
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:12
Gene Name:cysS
Confidence Code:2
GeneProduct:cysteinyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0215,cd00672,pfam01406
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:6.1.1.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57211.1| (AE016869) cysteinyl-tRNA synthetase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 460

 Score =  798 bits (2062), Expect = 0.0
 Identities = 380/460 (82%), Positives = 419/460 (91%)

>gb|AAN68513.1|AE016483_4 (AE016784) cysteinyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 460

 Score =  789 bits (2037), Expect = 0.0
 Identities = 379/460 (82%), Positives = 412/460 (89%)

>dbj|BAC59413.1| (AP005077) cysteinyl-tRNA synthetase [Vibrio parahaemolyticus]
          Length = 460

 Score =  563 bits (1450), Expect = e-159
 Identities = 267/458 (58%), Positives = 347/458 (75%), Gaps = 1/458 (0%)






 
Structural Features:
COG0215, CysS, Cysteinyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 464 residues,  99.6% aligned
Score =  696 bits (1798), Expect = 0.0
Identities = 278/464 (59%), Positives = 343/464 (73%), Gaps = 9/464 (1%)

cd00672, CysRS_core, This is the catalytic core domain of 
cysteinyl tRNA synthetase (CysRS). 
CD-Length = 303 residues, 100.0% aligned
Score =  475 bits (1224), Expect = 5e-135

pfam01406, tRNA-synt_1e, tRNA synthetases class I (C) catalytic domain.
CD-Length = 301 residues,  99.7% aligned
Score =  469 bits (1209), Expect = 2e-133
Identities = 182/300 (60%), Positives = 214/300 (71%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14261




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19019




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36518




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:937_cdd.html


    		




 
URL:

















    ReferenceID:36586




  
Author/Investigator(s):
        Avalos J, Corrochano LM, Brenner S.





  
Title:
        Cysteinyl-tRNA synthetase is a direct descendant of the first aminoacyl-tRNA synthetase.




  
PubMed:
        1864365


MedLine:
        




    
Source:
FEBS Lett. 1991 Jul 29;286(1-2):176-80.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=91323511&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:937
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1656
                           PA1795_tr
                           translation of PA1795
                           460
                           99.78
                           99.78
                           0
                           2413.0
     

     
     
59084
                           gb|AAG05184.1|AE004605_6
                           (AE004605) cysteinyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           460
                           99.56
                           99.78
                           0
                           2410.0
     

     
     
59085
                           gb|AAO57211.1|
                           (AE016869) cysteinyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           460
                           82.39
                           91.08
                           0
                           2058.0
     

     
     
59086
                           gb|AAN68513.1|AE016483_4
                           (AE016784) cysteinyl-tRNA synthetase [Pseudomonas putida KT2440]
                           460
                           82.17
                           89.56
                           0
                           2033.0
     

     
     
59095
                           gb|AAQ59420.1|
                           (AE016916) cysteinyl-tRNA synthetase [Chromobacterium violaceum ATCC 12472]
                           467
                           59.52
                           71.30
                           15
                           1436.0
     

     






      

          
GC ORFID: 43939How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1795Glimmer Score: 

          
Start: 1263980Stop: 1265362

          
Length: 1383


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1795 translation of PA1795Homolog Bit Score 934.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene763 (ORF: BLASTX 798.0), t_PA1795 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCTGCAGATCTACAACACGCTGAGCAAGACCAAAGAAGTCTTCACGCCGCTGGTCGGCAACCAGGTGCGC 
ATGTACGTGTGCGGCATGACCGTGTACGACTACTGCCATCTGGGCCACGGCCGCAGCATGGTGGCGTTCGAC 
GTGATCACCCGCTGGTTGCGCCACCGTGGCTACGACTTGACGTACGTGCGCAACATCACCGATATCGATGAC 
AAGATCATCAATCGCGCCAACGAGAACGACGAACCGTTCGATGTGCTGACCGAGCGCATGATCGCGGCGATG 
CACGAGGACGAGGCCCGGCTGAACATCCTCAAGCCGGACCAGGAGCCGCGCGCCACCGATCACATCGCCGGC 
ATGCACGCGATGATCCAGACCCTGATCGACAAGGGCTATGCCTACGCGCCGGGCAACGGTGACGTGTACTAC 
CGGGTCGGCAAGTTCGCTGGCTACGGCAAGTTGTCGCGGCGGCGGGTCGAGGACCTGCGCATCGGTGCGCGG 
ATCGAACCGGGCGAAGCGAAGGAGGATCCGCTCGATTTCGTGCTCTGGAAGGGCGCCAAGCCGGGCGAGCCG 
AGCTGGTCGTCGCCCTGGGGCGAGGGGCGCCCGGGCTGGCACATCGAATGCTCGGTGATGTCCACCTGCTGC 
CTGGGCGATTCCTTCGACATCCATGGCGGTGGCAACGACCTGGAGTTCCCTCACCACGAGAACGAGATCGCC 
CAGAGCGAGGCCGCGACTGGCAAGCCCTACGCCAAGTCCTGGCTGCACTGCGGGATGATTACCATCAACGGC 
GAGAAGATGTCCAAGTCGCTGGGCAATTTCTTCACCATCCGCGAAGTGCTGGAGAAATATCACCCCGAAGTG 
GTGCGTTACCTGCTGATCGCCAGCCATTACCGCAGTCCGATCAACTACTCGGAGGAGAACCTGCGCGAGGCC 
AAGGCGGCCCTGGACCGTTTCTACAACGCGCTCAAGGGGTTGCCCGAGGCGGCGCCGGCGGAAGCGGCGGAG 
TATGTCGAGCGCTTCGCCGCGGCGATGGACGACGACTTCAATACCGCGGGTGCCTGCTCGGTGCTGTTCGAG 
CTGGCGCGGGAGGTCAATCGTCTGCGCGAAAGCGACCTGTCGGCGGCGGCCGCGCTGGCCGCGCGCCTGAAG 
CAGTTGGCTGGCCTGCTGGGGGTGCTGCAGCTGGAGCCGGAAGCCTTCCTGCAGGCGGGCGCCGAGGGCAAG 
GTCGATGCGGCCGAGGTCGAGGCGTTGATCCAGGCGCGCCTGGAGGCGCGGGCGGCGAAGAACTGGGCGGAG 
TCCGACCGGATTCGCGACCAGCTCACCGCCATGGGCGTGGTGCTGGAGGATGGCAAGGGCGGCACTACCTGG 
CGCCTGGCCGACTGA