ViewGene
Locus Name:
GeneID:
Location:

GeneID:933
TrivialName: PA14_18790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18790
PAO1 Ortholog Locus:PA3521
Sequence Length:1476
Protein Length:491
Start: 1618641
Stop: 1620116
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06909.1|AE004772_7 (AE004772) probable outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 488/491 (99%), Positives = 489/491 (99%)
Sequence:
ATGAAGCCATACCTGCGGTCTTCGTTGAGCGCGCTGATCCTGCTGGGCGGCTGTGCCGCGGTGGGGCCCGAC
TATGCGCCGCCGAGCGCGTCCGCACCGGCCAGCTTCGGCGCGATGCCGGCGGGTATCGATGGCAGTGGCGTG
GAGATCGAGTGGTGGCGCGGGTTCGACGAGCCAGCCCTGGAGTCTCTGATCCAGCGGGCGCTCGCGGCGAAC
CTCGACATCGCCCTTGCCGGCGCGCGCCTGGACGAAGCGAAGGCGCTGCTGCGCGAGAACCGCGAGGAGTTC
CTGCCGCGCGGCGGGCCGGCGTTCGATTACCAGGCCCGGCGGCGCGGCGAGGTGGAAACACCGGCCGGCCAG
CAGCGCGACATCGAGACCTATCGCGGCGCGCTGGATGCTTCCTGGGAAATCGACCTGTTCGGCCGGGTGCGG
CGCTCGGTGGAGGCCGCCGAGGCGCAGGCGGGTTCGCGCGAGGCCCTGCTGCGCAACGTGCAGGCGAGCGTC
GCGGCTACGGTTGCCATGACCTGGTTCCAGCTACTGGGCATCGAGGCCGAACTTGCGGTGGTCCATGACATC
GCCGGCAACCAGCGCGACAGCCTGGAGATGGTCGAGCGCCTGGTCAGCGCCGGCTCCGCCCACGAGTTCGAC
CGGTTGCGCGCCGAAGCGTTATTGCACAACGTCGAGGCGGCCGTGCCCGACCTCGAACGGCGCCGCGCCGCC
ACCCGCAATGCGCTGGCCGTTCTCCTCGCGGAAGCCCCGCAGGCCTTCAGCCCGCCGGTCGCCCGCGCCTCC
GGGGAAAGGCTGACGCTCCGCACCCTCGGGGTCGGCGACCCGGCGGGACTGCTGGCGCGTCGCGCCGACATC
GCCGCGGCCGAACGCAACCTGGCGGCCGCGACCGCACGTATCGGCGTCGAGACCGCGGGGCTGTATCCGCAG
GTCGAGGTACGCGGCTCGATCGGCCTGGTCGCCGGCAATCTCGATGCTTTGGACGAGAGTGGTACGTCCTTC
AACGTCCTGAACCCGGTGATTCGCTGGGCGTTACTCGACCGCGGTCGCGTGCGGGCGCGGATCGCCGCCAGC
GAAGCCCGTGCGCAGGAGGCATTGATCCTCTACGACCGGACGGTACTGCGCGCCCTGCAGGAGACCGATGAT
GCCTTCAACGGCTATGGCGCTGCCGCTGACAGGCTCCGCCTGCGCCTGCTCGAGGCGACGGCCAATCGCGAG
GCGGCGCGCCTGGCCCGGGAACGCTTCGTCCAGGGGGACGGCGAGTACCTGGACGTGCTCGAGGCGGAACGC
TCGGACTACCTCAGCCGGCGCGCATTGAGCATCGCGCGCACCGAGCAGCGCCTTGCCGTCGTGGGTATCTAC
AAGGCGCTGGGGGGTGGCTGGGAGGCCTGCGCGGGGGCGCGGCGCTGTGGCGTGGCTACCGACGATACCTCT
CCAGGCGTGGCACGGCAGCGCGACAGTCGCTCCTGA
Translation:
MKPYLRSSLSALILLGGCAAVGPDYAPPSASAPASFGAMPAGIDGSGVEIEWWRGFDEPALESLIQRALAAN
LDIALAGARLDEAKALLRENREEFLPRGGPAFDYQARRRGEVETPAGQQRDIETYRGALDASWEIDLFGRVR
RSVEAAEAQAGSREALLRNVQASVAATVAMTWFQLLGIEAELAVVHDIAGNQRDSLEMVERLVSAGSAHEFD
RLRAEALLHNVEAAVPDLERRRAATRNALAVLLAEAPQAFSPPVARASGERLTLRTLGVGDPAGLLARRADI
AAAERNLAAATARIGVETAGLYPQVEVRGSIGLVAGNLDALDESGTSFNVLNPVIRWALLDRGRVRARIAAS
EARAQEALILYDRTVLRALQETDDAFNGYGAAADRLRLRLLEATANREAARLARERFVQGDGEYLDVLEAER
SDYLSRRALSIARTEQRLAVVGIYKALGGGWEACAGARRCGVATDDTSPGVARQRDSRS*
AnnotationID:942GeneID:933
AnnotatorUID: diggins
Modification Date/Time: 2005-07-18 20:08:45
Gene Name:
Confidence Code:3
GeneProduct:putative outer membrane efflux protein
Cell Localization:(6) Outer membrane protein
Synonyms:opmE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02321,COG1538
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM40739.1| (AE012244) outer membrane protein [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 474

 Score =  289 bits (740), Expect = 2e-77
 Identities = 183/478 (38%), Positives = 270/478 (56%), Gaps = 10/478 (2%)

>gb|AAN69029.1|AE016535_3 (AE016786) multidrug efflux RND outer membrane protein OprN
           [Pseudomonas putida KT2440]
          Length = 471

 Score =  270 bits (691), Expect = 1e-71
 Identities = 174/468 (37%), Positives = 256/468 (54%), Gaps = 12/468 (2%)







 
Structural Features:
pfam02321, OEP, Outer membrane efflux protein.
CD-Length = 188 residues,  96.3% aligned
Score = 84.7 bits (209), Expect = 2e-17
Identities = 53/181 (29%), Positives = 83/181 (45%), Gaps = 1/181 (0%)

COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / 
Intracellular trafficking and secretion]
CD-Length = 457 residues,  98.9% aligned
Score =  221 bits (563), Expect = 2e-58
Identities = 163/458 (35%), Positives = 227/458 (49%), Gaps = 6/458 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1869




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19011




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32045




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:933_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:933
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1652
                           PA3521_tr
                           translation of PA3521
                           491
                           99.38
                           99.59
                           0
                           2419.0
     

     
     
59012
                           gb|AAG06909.1|AE004772_7
                           (AE004772) probable outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
                           491
                           99.38
                           99.59
                           0
                           2419.0
     

     
     
59013
                           dbj|BAC24098.1|
                           (AB085583) putative outer membrane efflux protein [Pseudomonas aeruginosa]
                           491
                           99.38
                           99.59
                           0
                           2419.0
     

     






      

          
GC ORFID: 43935How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3521Glimmer Score: 

          
Start: 3335293Stop: 3333818

          
Length: 1476


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3521 translation of PA3521Homolog Bit Score 936.0

          
Other Homologs:  t_PA3521 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCCATACCTGCGGTCTTCGTTGAGCGCGCTGATCCTGCTGGGCGGCTGTGCCGCGGTGGGGCCCGAC 
TATGCGCCGCCGAGCGCGTCCGCACCGGCCAGCTTCGGCGCGATGCCGGCGGGTATCGATGGCAGTGGCGTG 
GAGATCGAGTGGTGGCGCGGGTTCGACGAGCCAGCCCTGGAGTCTCTGATCCAGCGGGCGCTCGCGGCGAAC 
CTCGACATCGCCCTTGCCGGCGCGCGCCTGGACGAAGCGAAGGCGCTGCTGCGCGAGAACCGCGAGGAGTTC 
CTGCCGCGCGGCGGGCCGGCGTTCGATTACCAGGCCCGGCGGCGCGGCGAGGTGGAAACACCGGCCGGCCAG 
CAGCGCGACATCGAGACCTATCGCGGCGCGCTGGATGCTTCCTGGGAAATCGACCTGTTCGGCCGGGTGCGG 
CGCTCGGTGGAGGCCGCCGAGGCGCAGGCGGGTTCGCGCGAGGCCCTGCTGCGCAACGTGCAGGCGAGCGTC 
GCGGCTACGGTTGCCATGACCTGGTTCCAGCTACTGGGCATCGAGGCCGAACTTGCGGTGGTCCATGACATC 
GCCGGCAACCAGCGCGACAGCCTGGAGATGGTCGAGCGCCTGGTCAGCGCCGGCTCCGCCCACGAGTTCGAC 
CGGTTGCGCGCCGAAGCGTTATTGCACAACGTCGAGGCGGCCGTGCCCGACCTCGAACGGCGCCGCGCCGCC 
ACCCGCAATGCGCTGGCCGTTCTCCTCGCGGAAGCCCCGCAGGCCTTCAGCCCGCCGGTCGCCCGCGCCTCC 
GGGGAAAGGCTGACGCTCCGCACCCTCGGGGTCGGCGACCCGGCGGGACTGCTGGCGCGTCGCGCCGACATC 
GCCGCGGCCGAACGCAACCTGGCGGCCGCGACCGCACGTATCGGCGTCGAGACCGCGGGGCTGTATCCGCAG 
GTCGAGGTACGCGGCTCGATCGGCCTGGTCGCCGGCAATCTCGATGCTTTGGACGAGAGTGGTACGTCCTTC 
AACGTCCTGAACCCGGTGATTCGCTGGGCGTTACTCGACCGCGGTCGCGTGCGGGCGCGGATCGCCGCCAGC 
GAAGCCCGTGCGCAGGAGGCATTGATCCTCTACGACCGGACGGTACTGCGCGCCCTGCAGGAGACCGATGAT 
GCCTTCAACGGCTATGGCGCTGCCGCTGACAGGCTCCGCCTGCGCCTGCTCGAGGCGACGGCCAATCGCGAG 
GCGGCGCGCCTGGCCCGGGAACGCTTCGTCCAGGGGGACGGCGAGTACCTGGACGTGCTCGAGGCGGAACGC 
TCGGACTACCTCAGCCGGCGCGCATTGAGCATCGCGCGCACCGAGCAGCGCCTTGCCGTCGTGGGTATCTAC 
AAGGCGCTGGGGGGTGGCTGGGAGGCCTGCGCGGGGGCGCGGCGCTGTGGCGTGGCTACCGACGATACCTCT 
CCAGGCGTGGCACGGCAGCGCGACAGTCGCTCCTGA