ViewGene
Locus Name:
GeneID:
Location:

GeneID:915
TrivialName: PA14_56240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56240
PAO1 Ortholog Locus:PA4329
Sequence Length:1452
Protein Length:483
Start: 5020400
Stop: 5021851
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07717.1|AE004849_4 (AE004849) pyruvate kinase II [Pseudomonas aeruginosa PAO1]
Identities = 482/483 (99%), Positives = 483/483 (100%)
Sequence:
ATGTCCGTTCGCCGCACCAAAATCGTCGCCACCCTCGGCCCAGCCAGCAATTCCCCGGAAGTGCTGGAGCAA
CTGATCCTGGCCGGGATCGACGTCGCCCGCCTCAACTTCTCCCACGGCACCCCCGATGAACACCGCGCCCGC
GCCAGGCTGGTGCGCGAACTGGCGGCCAAGCACGGCCGCTTCGTCGCCCTGCTCGGCGACCTGCAAGGACCG
AAGATCCGCATCGCCAAGTTCGCCAACAAGCGCATCGAATTGCAGGTCGGCGACAAGTTCCGCTTCTCCACC
AGCCACGCCCGCGACGCCGGTACCCAGGAAGTGGTCGGCATCGACTACCCGGACCTGGTGAAGGACTGCGGG
GTCGGCGATGAACTGCTGCTGGACGACGGCAGGGTGGTGATGGTGGTCGAGGAAGTCGCCGCCGACGAACTG
CGCTGCCGGGTGCTGATCGGCGGCCCGCTGTCCGACCACAAGGGCATCAACCGCCGCGGCGGCGGTCTCACC
GCGCCGGCGCTGACCGACAAGGACAAGGCCGACATCAAGCTGGCCGCGGACATGGACCTGGACTACGTGGCC
GTTTCCTTCCCGCGCGACGCCAAGGACATGGAATACGCCCGCCGCCTGCTCACCGAGGCCGGAGGCAAGGCC
TGGCTGGTAGCCAAGATCGAGCGCGCCGAGGCGGTCGCCGACGACGACGCCCTCGACGGCCTGATTCGTGCC
AGCGACGCGGTGATGGTGGCCCGTGGCGACCTCGGCGTGGAAATCGGCGATGCCGAGCTGGTCGGCATCCAG
AAGAAGATCATCCTGCACGCCCGCCGCAACAATAAAGTGGTGATCACCGCGACCCAGATGATGGAGTCGATG
ATCCACAGCCCGATGCCGACCCGCGCGGAAGTCTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCG
GTGATGCTCTCGGCGGAAAGCGCCGCCGGCGAGTACCCGGTGGAAGCGGTGCAAGCCATGGCCCGCGTCTGC
CAGGGCGCCGAGAAGCACCCGACCAGCCAGAAATCCAGCCACCGCCTCGGCCAGACTTTCGACCGCTGCGAC
GAGAGCATCGCCCTGGCGTCGATGTACACGGCCAACCACTTCCCGGGGATCAAGGCGATCATCTGCCTGACC
GAAAGCGGCTTCACCCCGCTGATCATGTCGCGCATCCGCTCGTCGGTACCGATCTACGCCTACTCCCCGCAC
CGCGAGACCCAGGCTCGCGTGGCGATGTTCCGCGGCGTGGAAACCATCCCCTTCGACCCGGCGGCGCTGCCG
GCGGAGAAGGTCAGCCAGGCCGCGGTCGACGAGTTGCTCAAGCGCGGCGTGGTGACCAAGGGCGACTGGGTG
ATCCTGACCAAGGGCGACAGCTACACCGCCCAGGGCGGCACCAACACCATGAAGGTGCTGCACGTCGGCGAC
CTGCTGGTATAA
Translation:
MSVRRTKIVATLGPASNSPEVLEQLILAGIDVARLNFSHGTPDEHRARARLVRELAAKHGRFVALLGDLQGP
KIRIAKFANKRIELQVGDKFRFSTSHARDAGTQEVVGIDYPDLVKDCGVGDELLLDDGRVVMVVEEVAADEL
RCRVLIGGPLSDHKGINRRGGGLTAPALTDKDKADIKLAADMDLDYVAVSFPRDAKDMEYARRLLTEAGGKA
WLVAKIERAEAVADDDALDGLIRASDAVMVARGDLGVEIGDAELVGIQKKIILHARRNNKVVITATQMMESM
IHSPMPTRAEVSDVANAVLDYTDAVMLSAESAAGEYPVEAVQAMARVCQGAEKHPTSQKSSHRLGQTFDRCD
ESIALASMYTANHFPGIKAIICLTESGFTPLIMSRIRSSVPIYAYSPHRETQARVAMFRGVETIPFDPAALP
AEKVSQAAVDELLKRGVVTKGDWVILTKGDSYTAQGGTNTMKVLHVGDLLV*
AnnotationID:924GeneID:915
AnnotatorUID: danlee
Modification Date/Time: 2006-03-29 14:02:16
Gene Name:pykA
Confidence Code:2
GeneProduct:pyruvate kinase II
Cell Localization:(1) Cytoplasmic
Synonyms:PA4329, pyk-II
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:Phosphoenolpyruvate kinase
Functional Category Confidence Code:5
COGs:cd00288,pfam00224
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57788.1| (AE016871) pyruvate kinase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 483

 Score =  801 bits (2070), Expect = 0.0
 Identities = 402/483 (83%), Positives = 441/483 (91%)

>gb|AAN66985.1|AE016326_11 (AE016779) pyruvate kinase II [Pseudomonas putida KT2440]
          Length = 484

 Score =  798 bits (2060), Expect = 0.0
 Identities = 403/483 (83%), Positives = 437/483 (90%)

>emb|CAE34461.1| (BX640449) pyruvate kinase [Bordetella bronchiseptica]
          Length = 480

 Score =  686 bits (1771), Expect = 0.0
 Identities = 345/476 (72%), Positives = 400/476 (84%)








 
Structural Features:
cd00288, Pyruvate_Kinase, Pyruvate kinase (PK)

pfam00224, PK, Pyruvate kinase, barrel domain.






   
Genomic Context:






   
Comment:


















    ReferenceID:1833




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18975




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36685




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:915_cdd.html


    		




 
URL:

















    ReferenceID:36804




  
Author/Investigator(s):
        Ponce E, Flores N, Martinez A, Valle F, Bolivar F.





  
Title:
        Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.




  
PubMed:
        7559366


MedLine:
        




    
Source:
J Bacteriol. 1995 Oct;177(19):5719-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7559366

















    ReferenceID:36805




  
Author/Investigator(s):
        Munoz ME, Le Borgne S, Bolivar F, Valle F.





  
Title:
        Molecular cloning of the gene that codes for the pyruvate kinase of Bacillus subtilis: primary characterization of a strain carrying this gene insertionally inactivated.




  
PubMed:
        10932722


MedLine:
        




    
Source:
Rev Latinoam Microbiol. 1997 Jul-Dec;39(3-4):129-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10932722









           
Homologs By Global Alignment 

           

       



     
Gene ID:915
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1625
                           PA4329_tr
                           translation of PA4329
                           483
                           99.79
                           99.99
                           0
                           2429.0
     

     
     
58618
                           gb|AAG07717.1|AE004849_4
                           (AE004849) pyruvate kinase II [Pseudomonas aeruginosa PAO1]
                           483
                           99.79
                           99.99
                           0
                           2429.0
     

     
     
58620
                           gb|AAN66985.1|AE016326_11
                           (AE016779) pyruvate kinase II [Pseudomonas putida KT2440]
                           484
                           83.26
                           90.28
                           1
                           2060.0
     

     
     
58619
                           gb|AAO57788.1|
                           (AE016871) pyruvate kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           483
                           83.22
                           91.30
                           0
                           2070.0
     

     
     
58622
                           emb|CAE38947.1|
                           (BX640434) pyruvate kinase [Bordetella parapertussis]
                           484
                           71.69
                           82.85
                           5
                           1766.0
     

     






      

          
GC ORFID: 43917How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4329Glimmer Score: 

          
Start: 4284076Stop: 4285527

          
Length: 1452


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4329 translation of PA4329Homolog Bit Score 940.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene1952 (ORF: BLASTX 805.0), t_PA4329 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCGTTCGCCGCACCAAAATCGTCGCCACCCTCGGCCCAGCCAGCAATTCCCCGGAAGTGCTGGAGCAA 
CTGATCCTGGCCGGGATCGACGTCGCCCGCCTCAACTTCTCCCACGGCACCCCCGATGAACACCGCGCCCGC 
GCCAGGCTGGTGCGCGAACTGGCGGCCAAGCACGGCCGCTTCGTCGCCCTGCTCGGCGACCTGCAAGGACCG 
AAGATCCGCATCGCCAAGTTCGCCAACAAGCGCATCGAATTGCAGGTCGGCGACAAGTTCCGCTTCTCCACC 
AGCCACGCCCGCGACGCCGGTACCCAGGAAGTGGTCGGCATCGACTACCCGGACCTGGTGAAGGACTGCGGG 
GTCGGCGATGAACTGCTGCTGGACGACGGCAGGGTGGTGATGGTGGTCGAGGAAGTCGCCGCCGACGAACTG 
CGCTGCCGGGTGCTGATCGGCGGCCCGCTGTCCGACCACAAGGGCATCAACCGCCGCGGCGGCGGTCTCACC 
GCGCCGGCGCTGACCGACAAGGACAAGGCCGACATCAAGCTGGCCGCGGACATGGACCTGGACTACGTGGCC 
GTTTCCTTCCCGCGCGACGCCAAGGACATGGAATACGCCCGCCGCCTGCTCACCGAGGCCGGAGGCAAGGCC 
TGGCTGGTAGCCAAGATCGAGCGCGCCGAGGCGGTCGCCGACGACGACGCCCTCGACGGCCTGATTCGTGCC 
AGCGACGCGGTGATGGTGGCCCGTGGCGACCTCGGCGTGGAAATCGGCGATGCCGAGCTGGTCGGCATCCAG 
AAGAAGATCATCCTGCACGCCCGCCGCAACAATAAAGTGGTGATCACCGCGACCCAGATGATGGAGTCGATG 
ATCCACAGCCCGATGCCGACCCGCGCGGAAGTCTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCG 
GTGATGCTCTCGGCGGAAAGCGCCGCCGGCGAGTACCCGGTGGAAGCGGTGCAAGCCATGGCCCGCGTCTGC 
CAGGGCGCCGAGAAGCACCCGACCAGCCAGAAATCCAGCCACCGCCTCGGCCAGACTTTCGACCGCTGCGAC 
GAGAGCATCGCCCTGGCGTCGATGTACACGGCCAACCACTTCCCGGGGATCAAGGCGATCATCTGCCTGACC 
GAAAGCGGCTTCACCCCGCTGATCATGTCGCGCATCCGCTCGTCGGTACCGATCTACGCCTACTCCCCGCAC 
CGCGAGACCCAGGCTCGCGTGGCGATGTTCCGCGGCGTGGAAACCATCCCCTTCGACCCGGCGGCGCTGCCG 
GCGGAGAAGGTCAGCCAGGCCGCGGTCGACGAGTTGCTCAAGCGCGGCGTGGTGACCAAGGGCGACTGGGTG 
ATCCTGACCAAGGGCGACAGCTACACCGCCCAGGGCGGCACCAACACCATGAAGGTGCTGCACGTCGGCGAC 
CTGCTGGTATAA