ViewGene
Locus Name:
GeneID:
Location:

GeneID:914
TrivialName: PA14_21410
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21410
PAO1 Ortholog Locus:PA3296
Sequence Length:1431
Protein Length:476
Start: 1857703
Stop: 1856273
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06684.1|AE004751_10 (AE004751) alkaline phosphatase [Pseudomonas aeruginosa PAO1]
Identities = 474/476 (99%), Positives = 475/476 (99%)
Sequence:
ATGACCCCAGGTTATCCCCTCGCCCTCTCTCTTGCCGTCTCCATGGCCGTGCTCGGCAGCGCCTTGCCGGCC
CAGGCGCGCCAGGACGATCCGTCGCTGTTCAACCGCCAGGCCCGTGGCGAACTCAGCGAGTACGGCGGCGCA
CGGCGCGTCGAGCAGGACCTGACCCAGGCCCTGAAGCAGTCGCTGTCGAAGAAGAAGGCGAAGAACGTGATC
CTGCTGATCGGCGACGGCATGGGCGACTCCGAGATCACCGTGGCGCGCAACTACGCGCGCGGCGCGGGCGGC
TACTTCAAGGGTATCGATGCGCTGCCGCTGACCGGCCAGTACACCCACTACTCCCTGCACAAGGACAGCGGC
CTGCCGGACTACGTGACCGATTCCGCCGCCTCCGCCACCGCCTGGACCACCGGGGTCAAGTCGTACAACGGC
GCAATCGGCGTGGATATCCACGAACAGCCACACCGCAACCTGCTGGAGCTGGCCAAGCTCAACGGCAAGGCC
ACCGGCAACGTCTCCACCGCCGAGCTGCAGGACGCCACCCCGGCCGCCCTGCTCGCCCACGTCACCGCTCGC
AAGTGCTACGGCCCCGAAGCCACCAGCAAGCAGTGCCCGAGCAATGCCCTGGAGAACGGCGGCGCCGGCTCG
ATCACCGAGCAATGGCTGAAGACCCGCCCTGACGTGGTCCTCGGCGGCGGCGCCGCGACCTTCGCGGAAACC
GCCAAGGCTGGCCGCTATGCCGGCAAGACCCTCCGCGCCCAGGCCGAGGCCCGCGGCTACCGGATCGTCGAG
AACCTCGACGAGCTGAAAGCCGTGCGCCGCGCCAACCAGAAGCAGCCGCTGATCGGCCTGTTCGCGCCGGGC
AACATGCCGGTGCGCTGGCTCGGTCCGACCGCCACCTACCACGGCAACCTGAACCAGCCGGCGGTGAGCTGC
GAGGCGAACCCGAAGCGCACCGCCGACATCCCGACCCTGGCGCAAATGACCAGCAAGGCCATCGAGCTGCTG
AAGGACAATCCGAACGGCTTCTTCCTGCAGGTCGAGGGCGCGTCCATCGACAAACAGGACCACGCCGCGAAT
CCGTGCGGCCAGATCGGCGAGACCGTCGACCTCGACGAAGCCGTGCAGAAGGCCCTGGCCTTCGCCAAGGCC
GATGGCGAGACCCTGGTGATCGTCACCGCCGACCACGCCCACTCCAGCCAGATCATCCCGCCGGAAACCGCC
GCGCCGGGGCTGACCCAACTGCTCACGACCAAGGACGGCGCGCCGCTGGCGATCAGCTACGGCAACTCCGAG
GAAGGCTCCCAGGAGCACACCGGCACCCAGTTGCGCATCGCCGCCTACGGCCCGCAGGCCGCCAATGTCACC
GGCCTGACCGACCAGACCGACCTGTTCTTCACCATCCGTCGCGCACTGAACCTGCGCGACTGA
Translation:
MTPGYPLALSLAVSMAVLGSALPAQARQDDPSLFNRQARGELSEYGGARRVEQDLTQALKQSLSKKKAKNVI
LLIGDGMGDSEITVARNYARGAGGYFKGIDALPLTGQYTHYSLHKDSGLPDYVTDSAASATAWTTGVKSYNG
AIGVDIHEQPHRNLLELAKLNGKATGNVSTAELQDATPAALLAHVTARKCYGPEATSKQCPSNALENGGAGS
ITEQWLKTRPDVVLGGGAATFAETAKAGRYAGKTLRAQAEARGYRIVENLDELKAVRRANQKQPLIGLFAPG
NMPVRWLGPTATYHGNLNQPAVSCEANPKRTADIPTLAQMTSKAIELLKDNPNGFFLQVEGASIDKQDHAAN
PCGQIGETVDLDEAVQKALAFAKADGETLVIVTADHAHSSQIIPPETAAPGLTQLLTTKDGAPLAISYGNSE
EGSQEHTGTQLRIAAYGPQAANVTGLTDQTDLFFTIRRALNLRD*
AnnotationID:923GeneID:914
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 07:07:40
Gene Name:phoA
Confidence Code:2
GeneProduct:alkaline phosphatase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:alkaline phosphatase signal peptide
Functional Category Confidence Code:5
COGs:cd00016,smart00098,pfam00245,COG1785
Secondary Functional Category(ies):transport of small molecules
EC Number:3.1.3.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA26563.1| (M33965) alkaline phosphatase (phoA) (EC 3.1.3.1) [Serratia
           marcescens]
          Length = 475

 Score =  674 bits (1740), Expect = 0.0
 Identities = 340/475 (71%), Positives = 388/475 (81%)

>gb|AAA24363.1| (M29663) alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
           coli]
          Length = 471

 Score =  671 bits (1731), Expect = 0.0
 Identities = 330/445 (74%), Positives = 377/445 (84%)

>gb|AAM85692.1|AE013816_2 (AE013816) alkaline phosphatase [Yersinia pestis KIM]
          Length = 491

 Score =  663 bits (1711), Expect = 0.0
 Identities = 335/473 (70%), Positives = 391/473 (82%), Gaps = 1/473 (0%)








 
Structural Features:
COG1785, PhoA, Alkaline phosphatase 
[Inorganic ion transport and metabolism]
CD-Length = 482 residues,  99.6% aligned
Score =  432 bits (1112), Expect = 4e-122
Identities = 214/516 (41%), Positives = 260/516 (50%), Gaps = 87/516 (16%)

smart00098, alkPPc, Alkaline phosphatase homologues
CD-Length = 420 residues, 100.0% aligned
Score =  363 bits (934), Expect = 2e-101
Identities = 172/450 (38%), Positives = 217/450 (48%), Gaps = 73/450 (16%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1831




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18973




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32257




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:914_cdd.html


    		




 
URL:

















    ReferenceID:34015




  
Author/Investigator(s):
        Kamitani S, Akiyama Y, Ito K.





  
Title:
        Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme.




  
PubMed:
        1740115


MedLine:
        




    
Source:
EMBO J. 1992 Jan;11(1):57-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1740115

















    ReferenceID:34016




  
Author/Investigator(s):
        Chang CN, Kuang WJ, Chen EY.





  
Title:
        Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli.




  
PubMed:
        3533724


MedLine:
        




    
Source:
Gene. 1986;44(1):121-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3533724

















    ReferenceID:34017




  
Author/Investigator(s):
        Inouye H, Barnes W, Beckwith J.





  
Title:
        Signal sequence of alkaline phosphatase of Escherichia coli.




  
PubMed:
        7035431


MedLine:
        




    
Source:
J Bacteriol. 1982 Feb;149(2):434-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7035431

















    ReferenceID:34018




  
Author/Investigator(s):
        Kikuchi Y, Yoda K, Yamasaki M, Tamura G.





  
Title:
        The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli.




  
PubMed:
        6273802


MedLine:
        




    
Source:
Nucleic Acids Res. 1981 Nov 11;9(21):5671-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6273802









           
Homologs By Global Alignment 

           

       



     
Gene ID:914
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1624
                           PA3296_tr
                           translation of PA3296
                           476
                           99.57
                           99.78
                           0
                           2430.0
     

     
     
58586
                           gb|AAG06684.1|AE004751_10
                           (AE004751) alkaline phosphatase [Pseudomonas aeruginosa PAO1]
                           476
                           99.57
                           99.78
                           0
                           2430.0
     

     
     
58587
                           gb|AAA26563.1|
                           (M33965) alkaline phosphatase (phoA) (EC 3.1.3.1) [Serratia marcescens]
                           476
                           71.42
                           81.51
                           1
                           1740.0
     

     
     
58588
                           gb|AAA24371.1|
                           (M33966) alkaline phosphatase (phoA) (EC 3.1.3.1) [Escherichia fergusonii]
                           476
                           70.79
                           80.67
                           4
                           1727.0
     

     
     
58589
                           gb|AAA24363.1|
                           (M29663) alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia coli]
                           476
                           70.16
                           81.51
                           5
                           1725.0
     

     






      

          
GC ORFID: 43916How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3296Glimmer Score: 

          
Start: 3096228Stop: 3097658

          
Length: 1431


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3296 translation of PA3296Homolog Bit Score 940.0

          
Other Homologs:  t_PA3296 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCCAGGTTATCCCCTCGCCCTCTCTCTTGCCGTCTCCATGGCCGTGCTCGGCAGCGCCTTGCCGGCC 
CAGGCGCGCCAGGACGATCCGTCGCTGTTCAACCGCCAGGCCCGTGGCGAACTCAGCGAGTACGGCGGCGCA 
CGGCGCGTCGAGCAGGACCTGACCCAGGCCCTGAAGCAGTCGCTGTCGAAGAAGAAGGCGAAGAACGTGATC 
CTGCTGATCGGCGACGGCATGGGCGACTCCGAGATCACCGTGGCGCGCAACTACGCGCGCGGCGCGGGCGGC 
TACTTCAAGGGTATCGATGCGCTGCCGCTGACCGGCCAGTACACCCACTACTCCCTGCACAAGGACAGCGGC 
CTGCCGGACTACGTGACCGATTCCGCCGCCTCCGCCACCGCCTGGACCACCGGGGTCAAGTCGTACAACGGC 
GCAATCGGCGTGGATATCCACGAACAGCCACACCGCAACCTGCTGGAGCTGGCCAAGCTCAACGGCAAGGCC 
ACCGGCAACGTCTCCACCGCCGAGCTGCAGGACGCCACCCCGGCCGCCCTGCTCGCCCACGTCACCGCTCGC 
AAGTGCTACGGCCCCGAAGCCACCAGCAAGCAGTGCCCGAGCAATGCCCTGGAGAACGGCGGCGCCGGCTCG 
ATCACCGAGCAATGGCTGAAGACCCGCCCTGACGTGGTCCTCGGCGGCGGCGCCGCGACCTTCGCGGAAACC 
GCCAAGGCTGGCCGCTATGCCGGCAAGACCCTCCGCGCCCAGGCCGAGGCCCGCGGCTACCGGATCGTCGAG 
AACCTCGACGAGCTGAAAGCCGTGCGCCGCGCCAACCAGAAGCAGCCGCTGATCGGCCTGTTCGCGCCGGGC 
AACATGCCGGTGCGCTGGCTCGGTCCGACCGCCACCTACCACGGCAACCTGAACCAGCCGGCGGTGAGCTGC 
GAGGCGAACCCGAAGCGCACCGCCGACATCCCGACCCTGGCGCAAATGACCAGCAAGGCCATCGAGCTGCTG 
AAGGACAATCCGAACGGCTTCTTCCTGCAGGTCGAGGGCGCGTCCATCGACAAACAGGACCACGCCGCGAAT 
CCGTGCGGCCAGATCGGCGAGACCGTCGACCTCGACGAAGCCGTGCAGAAGGCCCTGGCCTTCGCCAAGGCC 
GATGGCGAGACCCTGGTGATCGTCACCGCCGACCACGCCCACTCCAGCCAGATCATCCCGCCGGAAACCGCC 
GCGCCGGGGCTGACCCAACTGCTCACGACCAAGGACGGCGCGCCGCTGGCGATCAGCTACGGCAACTCCGAG 
GAAGGCTCCCAGGAGCACACCGGCACCCAGTTGCGCATCGCCGCCTACGGCCCGCAGGCCGCCAATGTCACC 
GGCCTGACCGACCAGACCGACCTGTTCTTCACCATCCGTCGCGCACTGAACCTGCGCGACTGA