ViewGene
Locus Name:
GeneID:
Location:

GeneID:913
TrivialName: PA14_62770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62770
PAO1 Ortholog Locus:PA4745
Sequence Length:1482
Protein Length:493
Start: 5603387
Stop: 5601906
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08131.1|AE004888_6 (AE004888) N utilization substance protein A [Pseudomonas aeruginosa PAO1]
Identities = 493/493 (100%), Positives = 493/493 (100%)
Sequence:
ATGAGCAAAGAAGTACTGCTGGTTGTTGAGTCGGTATCCAACGAAAAGGGCGTACCGGCCGGCGTGATTTTC
GAGGCGCTGGAGCTGGCTCTGGCGACCGCCACCAAGAAACGTTTCGAGGACGAGGTCGACCTGCGCGTCGAG
ATCAATCGTCATAACGGCAGCTACGAGACTTTCCGTCGTTGGCACGTCGTCGCCGACGAGGACTACCAGGAC
CCCGCCACCGAGATCACCGTCGAGGACGTCCAGGAGCAGAAGCCGGGCGCGAAGGTCGGCGAGGTCATCGAA
GAAAAGATCGAATCCATCGAGTTCGGCCGCATCGCTGCGCAGACTGCCAAGCAGGTCATCGTGCAGAAGGTC
CGCGAGGCCGAGCGCGCCCAGGTGGTCGATGCCTACCGCGAGAAGGTCGGCGAGATCATTTCCGGTACCGTG
AAGAAGGTCACCCGCGACAACGTCATCGTCGATCTCGGCAACAACGCCGAGGCGCTGCTGGCCCGCGACCAG
ATCATTCCGCGCGAGACCTTCCGCGTTGGCACCCGCGTGCGTGCCCTGCTGAAGGAGATCCGTACCGAGAAC
CGCGGTCCTCAGCTGGTCCTGTCGCGTACCGCGCCGGAAATGCTGATCGAGCTGTTCCGCATCGAAGTACCG
GAAATCGCCGAGCAGTTGATCGACGTGATGGCCGCCGCCCGTGACCCGGGCTCGCGCGCCAAGATCGCCGTT
CGTTCCAAGGACAAGCGCATCGACCCGCAGGGCGCCTGCATCGGCATGCGCGGTTCGCGCGTCCAGGCGGTA
TCCGGCGAGATCGGTGGAGAGCGGGTGGACATCGTCCTGTGGGACGACAACCCGGCGCAATTCGTGATCAAC
GCCATGGCTCCGGCCGAGGTGGCGGCGATCATCGTCGACGAGGATACCCATACCATGGATATCGCCGTCGCC
GAGGACAATCTGGCGCAGGCTATCGGCCGCAGTGGCCAGAACGTCCGTCTGGCCAGCCAGTTGACCGGCTGG
ACCCTGAATGTGATGACCGAGGCGGATATCCAGGCCAAGCAACAGGCCGAGACCGGGGATATCCTGCAGCGC
TTCGTCGACGAGCTGGATGTCGACGAGGAACTGGCCCAGGTACTGGTGGAAGAGGGCTTCACGACTCTTGAG
GAAATCGCCTACGTACCGATGGAAGAGATGCTCAGCATCGATGGCTTCGACGAAGACATCGTCAACGAGCTG
CGCTCCCGTGCCAAGGATCGCCTGCTGACCAAGGCCATTGCGACCGAAGAGAAGCTCGCCGACGCACAACCG
GCAGAAGACCTGCTCAGCCTCGATGGCATGAGCAAGGAGCTGGCCCTGGACCTGGCGCTGCGTGGCGTAACC
ACCCGTGAAGATCTGGCCGAGCAATCGATCGACGATCTGCTCGACATCGACGGCATGGACGAAGAGCGTGCC
GGCAAGTTGATCATGGCCGCCCGGGCCCATTGGTTCGAGTAA
Translation:
MSKEVLLVVESVSNEKGVPAGVIFEALELALATATKKRFEDEVDLRVEINRHNGSYETFRRWHVVADEDYQD
PATEITVEDVQEQKPGAKVGEVIEEKIESIEFGRIAAQTAKQVIVQKVREAERAQVVDAYREKVGEIISGTV
KKVTRDNVIVDLGNNAEALLARDQIIPRETFRVGTRVRALLKEIRTENRGPQLVLSRTAPEMLIELFRIEVP
EIAEQLIDVMAAARDPGSRAKIAVRSKDKRIDPQGACIGMRGSRVQAVSGEIGGERVDIVLWDDNPAQFVIN
AMAPAEVAAIIVDEDTHTMDIAVAEDNLAQAIGRSGQNVRLASQLTGWTLNVMTEADIQAKQQAETGDILQR
FVDELDVDEELAQVLVEEGFTTLEEIAYVPMEEMLSIDGFDEDIVNELRSRAKDRLLTKAIATEEKLADAQP
AEDLLSLDGMSKELALDLALRGVTTREDLAEQSIDDLLDIDGMDEERAGKLIMAARAHWFE*
AnnotationID:922GeneID:913
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 08:08:30
Gene Name:nusA
Confidence Code:2
GeneProduct:N utilization substance protein A
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:transcription pausing; L factor
Functional Category Confidence Code:5
COGs:COG0195
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70285.1|AE016669_3 (AE016791) N utilization substance protein A [Pseudomonas putida
           KT2440]
          Length = 493

 Score =  843 bits (2178), Expect = 0.0
 Identities = 435/493 (88%), Positives = 466/493 (94%)

>gb|AAO57939.1| (AE016872) N utilization substance protein A [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 493

 Score =  840 bits (2170), Expect = 0.0
 Identities = 432/493 (87%), Positives = 466/493 (94%)

>gb|AAG58305.1|AE005545_7 (AE005545) transcription pausing; L factor [Escherichia coli
           O157:H7 EDL933]
          Length = 495

 Score =  617 bits (1592), Expect = e-176
 Identities = 317/493 (64%), Positives = 391/493 (79%), Gaps = 3/493 (0%)






 
Structural Features:
COG0195, NusA, Transcription elongation factor [Transcription]
CD-Length = 190 residues, 100.0% aligned
Score =  180 bits (457), Expect = 4e-46
Identities = 97/196 (49%), Positives = 138/196 (70%), Gaps = 7/196 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1829




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18971




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37668




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:913_cdd.html


    		




 
URL:

















    ReferenceID:37726




  
Author/Investigator(s):
        Saito M, Tsugawa A, Egawa K, Nakamura Y.





  
Title:
        Revised sequence of the nusA gene of Escherichia coli and identification of nusA11 (ts) and nusA1 mutations which cause changes in a hydrophobic amino acid cluster.




  
PubMed:
        3027511


MedLine:
        




    
Source:
Mol Gen Genet. 1986 Nov;205(2):380-2.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3027511

















    ReferenceID:37727




  
Author/Investigator(s):
        Imamoto F, Nakamura Y.





  
Title:
        Escherichia coli proteins involved in regulation of transcription termination: function, structure, and expression of the nusA and nusB genes.




  
PubMed:
        3019094


MedLine:
        




    
Source:
Adv Biophys. 1986;21:175-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3019094

















    ReferenceID:37728




  
Author/Investigator(s):
        Ishii S, Ihara M, Maekawa T, Nakamura Y, Uchida H, Imamoto F.





  
Title:
        The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli.




  
PubMed:
        6326058


MedLine:
        




    
Source:
Nucleic Acids Res. 1984 Apr 11;12(7):3333-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6326058









           
Homologs By Global Alignment 

           

       



     
Gene ID:913
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1623
                           PA4745_tr
                           translation of PA4745
                           493
                           99.99
                           99.99
                           0
                           2431.0
     

     
     
58530
                           gb|AAG08131.1|AE004888_6
                           (AE004888) N utilization substance protein A [Pseudomonas aeruginosa PAO1]
                           493
                           99.99
                           99.99
                           0
                           2431.0
     

     
     
58531
                           gb|AAN70285.1|AE016669_3
                           (AE016791) N utilization substance protein A [Pseudomonas putida KT2440]
                           493
                           88.23
                           94.52
                           0
                           2178.0
     

     
     
58532
                           gb|AAO57939.1|
                           (AE016872) N utilization substance protein A [Pseudomonas syringae pv. tomato str. DC3000]
                           493
                           87.62
                           94.52
                           0
                           2170.0
     

     
     
58540
                           dbj|BAB37473.1|
                           (AP002564) transcription termination-antitermination factor NusA [Escherichia coli O157:H7]
                           497
                           63.78
                           78.67
                           6
                           1594.0
     

     






      

          
GC ORFID: 43915How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4745Glimmer Score: 

          
Start: 4867067Stop: 4865586

          
Length: 1482


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4745 translation of PA4745Homolog Bit Score 941.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5492 (ORF: BLASTX 840.0), t_PA4745 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAAGAAGTACTGCTGGTTGTTGAGTCGGTATCCAACGAAAAGGGCGTACCGGCCGGCGTGATTTTC 
GAGGCGCTGGAGCTGGCTCTGGCGACCGCCACCAAGAAACGTTTCGAGGACGAGGTCGACCTGCGCGTCGAG 
ATCAATCGTCATAACGGCAGCTACGAGACTTTCCGTCGTTGGCACGTCGTCGCCGACGAGGACTACCAGGAC 
CCCGCCACCGAGATCACCGTCGAGGACGTCCAGGAGCAGAAGCCGGGCGCGAAGGTCGGCGAGGTCATCGAA 
GAAAAGATCGAATCCATCGAGTTCGGCCGCATCGCTGCGCAGACTGCCAAGCAGGTCATCGTGCAGAAGGTC 
CGCGAGGCCGAGCGCGCCCAGGTGGTCGATGCCTACCGCGAGAAGGTCGGCGAGATCATTTCCGGTACCGTG 
AAGAAGGTCACCCGCGACAACGTCATCGTCGATCTCGGCAACAACGCCGAGGCGCTGCTGGCCCGCGACCAG 
ATCATTCCGCGCGAGACCTTCCGCGTTGGCACCCGCGTGCGTGCCCTGCTGAAGGAGATCCGTACCGAGAAC 
CGCGGTCCTCAGCTGGTCCTGTCGCGTACCGCGCCGGAAATGCTGATCGAGCTGTTCCGCATCGAAGTACCG 
GAAATCGCCGAGCAGTTGATCGACGTGATGGCCGCCGCCCGTGACCCGGGCTCGCGCGCCAAGATCGCCGTT 
CGTTCCAAGGACAAGCGCATCGACCCGCAGGGCGCCTGCATCGGCATGCGCGGTTCGCGCGTCCAGGCGGTA 
TCCGGCGAGATCGGTGGAGAGCGGGTGGACATCGTCCTGTGGGACGACAACCCGGCGCAATTCGTGATCAAC 
GCCATGGCTCCGGCCGAGGTGGCGGCGATCATCGTCGACGAGGATACCCATACCATGGATATCGCCGTCGCC 
GAGGACAATCTGGCGCAGGCTATCGGCCGCAGTGGCCAGAACGTCCGTCTGGCCAGCCAGTTGACCGGCTGG 
ACCCTGAATGTGATGACCGAGGCGGATATCCAGGCCAAGCAACAGGCCGAGACCGGGGATATCCTGCAGCGC 
TTCGTCGACGAGCTGGATGTCGACGAGGAACTGGCCCAGGTACTGGTGGAAGAGGGCTTCACGACTCTTGAG 
GAAATCGCCTACGTACCGATGGAAGAGATGCTCAGCATCGATGGCTTCGACGAAGACATCGTCAACGAGCTG 
CGCTCCCGTGCCAAGGATCGCCTGCTGACCAAGGCCATTGCGACCGAAGAGAAGCTCGCCGACGCACAACCG 
GCAGAAGACCTGCTCAGCCTCGATGGCATGAGCAAGGAGCTGGCCCTGGACCTGGCGCTGCGTGGCGTAACC 
ACCCGTGAAGATCTGGCCGAGCAATCGATCGACGATCTGCTCGACATCGACGGCATGGACGAAGAGCGTGCC 
GGCAAGTTGATCATGGCCGCCCGGGCCCATTGGTTCGAGTAA