ViewGene
Locus Name:
GeneID:
Location:

GeneID:906
TrivialName: PA14_05620
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05620
PAO1 Ortholog Locus:PA0432
Sequence Length:1410
Protein Length:469
Start: 498866
Stop: 497457
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03821.1|AE004480_5 (AE004480) S-adenosyl-L-homocysteine hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 468/469 (99%), Positives = 469/469 (100%)
Sequence:
ATGAGCGCTGTCATGACGCCCGCCGGTTTCACCGACTACAAGGTCGCCGACATCACCCTGGCCGCCTGGGGC
CGCCGCGAGCTGATCATCGCCGAATCGGAAATGCCCGCACTGATGGGCCTGCGCCGCAAGTATGCCGGCCAG
CAGCCGCTGAAAGGCGCGAAGATCCTCGGTTGCATCCACATGACCATCCAGACCGGCGTGCTGATCGAGACC
CTGGTCGCGCTGGGCGCCGAAGTCCGCTGGTCGAGCTGCAACATCTTCTCCACCCAGGACCAGGCCGCCGCC
GCCATCGCCGCAGCCGGCATCCCGGTGTTCGCCTGGAAGGGCGAGACCGAGGAAGAGTACGAATGGTGCATC
GAGCAGACCATCCTCAAGGACGGCCAGCCGTGGGACGCCAACATGGTGCTGGACGACGGCGGTGACCTGACC
GAGATCCTGCACAAGAAATACCCGCAGATGCTCGAGCGCATCCACGGCATCACCGAAGAGACCACCACCGGG
GTCCACCGCCTGCTCGACATGCTCAAGAACGGCACCCTGAAAGTCCCGGCGATCAACGTCAACGACTCGGTG
ACCAAGAGCAAGAACGACAACAAGTACGGCTGCCGCCATAGCCTCAACGACGCCATCAAGCGCGGCACCGAC
CACCTGCTGTCGGGCAAGCAGGCCCTGGTGATCGGCTACGGCGACGTGGGCAAGGGCTCCTCGCAGTCGCTG
CGCCAGGAAGGCATGATCGTCAAGGTCGCGGAAGTCGACCCGATCTGCGCCATGCAGGCCTGCATGGACGGC
TTCGAAGTCGTCTCGCCGTACAAGAACGGCATCAACGACGGCACCGAGGCCAGCATCGACGCCGCGCTGCTG
GGCAAGATCGACCTGATCGTCACCACCACCGGCAACGTCAACGTCTGCGACGCCAACATGCTCAAGGCGCTG
AAGAAACGCGCGGTGGTCTGCAACATCGGCCACTTCGACAACGAGATCGACACCGCCTTCATGCGCAAGAAC
TGGGCCTGGGAAGAGGTCAAGCCGCAGGTGCACAAGATCCACCGCACCGGCAAGGACGGTTTCGACGCGTAC
AACGACGACTACCTGATCCTTCTCGCTGAGGGCCGCCTGGTCAACCTGGGCAACGCAACCGGCCATCCGAGC
CGGATCATGGACGGTTCCTTCGCCAACCAGGTGCTGGCGCAGATCCACCTGTTCGAGCAGAAGTACGCCGAC
CTGCCGGCCGCCGAGAAGGCCAAGCGCCTGAGCGTCGAAGTGCTGCCGAAGAAGCTCGACGAGGAAGTGGCC
CTGGAGATGGTCAAGGGCTTCGGCGGCGTGGTCACCCAGCTGACCCCGAAACAGGCCGAGTACATCGGCGTG
AGCGTGGAAGGCCCGTTCAAGCCGGACACCTATCGCTACTAA
Translation:
MSAVMTPAGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKILGCIHMTIQTGVLIET
LVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFAWKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLT
EILHKKYPQMLERIHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSLNDAIKRGTD
HLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICAMQACMDGFEVVSPYKNGINDGTEASIDAALL
GKIDLIVTTTGNVNVCDANMLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGFDAY
NDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYADLPAAEKAKRLSVEVLPKKLDEEVA
LEMVKGFGGVVTQLTPKQAEYIGVSVEGPFKPDTYRY*
AnnotationID:915GeneID:906
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 09:09:58
Gene Name:sahH
Confidence Code:2
GeneProduct:S-adenosyl-L-homocysteine hydrolase
Cell Localization:()
Synonyms:ahcY
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:adenosylhomocysteinase
Functional Category Confidence Code:5
COGs:COG0499,pfam00670,cd00401
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:3.3.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58495.1| (AE016874) adenosylhomocysteinase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 469

 Score =  858 bits (2218), Expect = 0.0
 Identities = 422/469 (89%), Positives = 445/469 (94%)

>gb|AAN51665.1|AE011599_2 (AE011599) S-adenosylhomocysteine hydrolase [Leptospira interrogans
           serovar lai str. 56601]
          Length = 436

 Score =  486 bits (1250), Expect = e-136
 Identities = 264/469 (56%), Positives = 323/469 (68%), Gaps = 36/469 (7%)

>gb|AAO91518.1| (AE016966) adenosylhomocysteinase [Coxiella burnetii RSA 493]
          Length = 429

 Score =  481 bits (1239), Expect = e-135
 Identities = 259/460 (56%), Positives = 323/460 (70%), Gaps = 36/460 (7%)








 
Structural Features:
COG0499, SAM1, S-adenosylhomocysteine hydrolase [Coenzyme metabolism]
CD-Length = 420 residues, 100.0% aligned
Score =  572 bits (1476), Expect = 3e-164
Identities = 228/455 (50%), Positives = 288/455 (63%), Gaps = 37/455 (8%)

pfam05221, AdoHcyase, S-adenosyl-L-homocysteine hydrolase.
CD-Length = 430 residues, 100.0% aligned
Score =  570 bits (1471), Expect = 9e-164
Identities = 261/460 (56%), Positives = 316/460 (68%), Gaps = 32/460 (6%)

cd00401, AdoHcyase, S-adenosyl-L-homocysteine hydrolase (AdoHycase)
CD-Length = 413 residues, 100.0% aligned
Score =  542 bits (1397), Expect = 4e-155
Identities = 257/445 (57%), Positives = 310/445 (69%), Gaps = 33/445 (7%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1815




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18957




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33756




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:906_cdd.html


    		




 
URL:

















    ReferenceID:35353




  
Author/Investigator(s):
        Bujnicki JM, Prigge ST, Caridha D, Chiang PK.





  
Title:
        Structure, evolution, and inhibitor interaction of S-adenosyl-L-homocysteine hydrolase from Plasmodium falciparum.




  
PubMed:
        12910461


MedLine:
        




    
Source:
Proteins. 2003 Sep 1;52(4):624-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12910461

















    ReferenceID:35354




  
Author/Investigator(s):
        Creedon KA, Rathod PK, Wellems TE.





  
Title:
        Plasmodium falciparum S-adenosylhomocysteine hydrolase. cDNA identification, predicted protein sequence, and expression in Escherichia coli.




  
PubMed:
        8206944


MedLine:
        




    
Source:
J Biol Chem. 1994 Jun 10;269(23):16364-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8206944









           
Homologs By Global Alignment 

           

       



     
Gene ID:906
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1611
                           PA0432_tr
                           translation of PA0432
                           469
                           99.78
                           99.99
                           0
                           2437.0
     

     
     
58365
                           gb|AAG03821.1|AE004480_5
                           (AE004480) S-adenosyl-L-homocysteine hydrolase [Pseudomonas aeruginosa PAO1]
                           469
                           99.78
                           99.99
                           0
                           2437.0
     

     
     
58366
                           gb|AAO58495.1|
                           (AE016874) adenosylhomocysteinase [Pseudomonas syringae pv. tomato str. DC3000]
                           469
                           89.97
                           94.88
                           0
                           2218.0
     

     
     
58367
                           gb|AAN51665.1|AE011599_2
                           (AE011599) S-adenosylhomocysteine hydrolase [Leptospira interrogans serovar lai str. 56601]
                           472
                           56.35
                           68.64
                           39
                           1280.0
     

     
     
58368
                           gb|AAO91518.1|
                           (AE016966) adenosylhomocysteinase [Coxiella burnetii RSA 493]
                           472
                           55.50
                           68.85
                           46
                           1267.0
     

     






      

          
GC ORFID: 43908How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0432Glimmer Score: 

          
Start: 6300208Stop: 6298799

          
Length: 1410


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0432 translation of PA0432Homolog Bit Score 943.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3733 (ORF: BLASTX 866.0), t_PA0432 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCTGTCATGACGCCCGCCGGTTTCACCGACTACAAGGTCGCCGACATCACCCTGGCCGCCTGGGGC 
CGCCGCGAGCTGATCATCGCCGAATCGGAAATGCCCGCACTGATGGGCCTGCGCCGCAAGTATGCCGGCCAG 
CAGCCGCTGAAAGGCGCGAAGATCCTCGGTTGCATCCACATGACCATCCAGACCGGCGTGCTGATCGAGACC 
CTGGTCGCGCTGGGCGCCGAAGTCCGCTGGTCGAGCTGCAACATCTTCTCCACCCAGGACCAGGCCGCCGCC 
GCCATCGCCGCAGCCGGCATCCCGGTGTTCGCCTGGAAGGGCGAGACCGAGGAAGAGTACGAATGGTGCATC 
GAGCAGACCATCCTCAAGGACGGCCAGCCGTGGGACGCCAACATGGTGCTGGACGACGGCGGTGACCTGACC 
GAGATCCTGCACAAGAAATACCCGCAGATGCTCGAGCGCATCCACGGCATCACCGAAGAGACCACCACCGGG 
GTCCACCGCCTGCTCGACATGCTCAAGAACGGCACCCTGAAAGTCCCGGCGATCAACGTCAACGACTCGGTG 
ACCAAGAGCAAGAACGACAACAAGTACGGCTGCCGCCATAGCCTCAACGACGCCATCAAGCGCGGCACCGAC 
CACCTGCTGTCGGGCAAGCAGGCCCTGGTGATCGGCTACGGCGACGTGGGCAAGGGCTCCTCGCAGTCGCTG 
CGCCAGGAAGGCATGATCGTCAAGGTCGCGGAAGTCGACCCGATCTGCGCCATGCAGGCCTGCATGGACGGC 
TTCGAAGTCGTCTCGCCGTACAAGAACGGCATCAACGACGGCACCGAGGCCAGCATCGACGCCGCGCTGCTG 
GGCAAGATCGACCTGATCGTCACCACCACCGGCAACGTCAACGTCTGCGACGCCAACATGCTCAAGGCGCTG 
AAGAAACGCGCGGTGGTCTGCAACATCGGCCACTTCGACAACGAGATCGACACCGCCTTCATGCGCAAGAAC 
TGGGCCTGGGAAGAGGTCAAGCCGCAGGTGCACAAGATCCACCGCACCGGCAAGGACGGTTTCGACGCGTAC 
AACGACGACTACCTGATCCTTCTCGCTGAGGGCCGCCTGGTCAACCTGGGCAACGCAACCGGCCATCCGAGC 
CGGATCATGGACGGTTCCTTCGCCAACCAGGTGCTGGCGCAGATCCACCTGTTCGAGCAGAAGTACGCCGAC 
CTGCCGGCCGCCGAGAAGGCCAAGCGCCTGAGCGTCGAAGTGCTGCCGAAGAAGCTCGACGAGGAAGTGGCC 
CTGGAGATGGTCAAGGGCTTCGGCGGCGTGGTCACCCAGCTGACCCCGAAACAGGCCGAGTACATCGGCGTG 
AGCGTGGAAGGCCCGTTCAAGCCGGACACCTATCGCTACTAA