ViewGene
Locus Name:
GeneID:
Location:

GeneID:905
TrivialName: PA14_34960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_34960
PAO1 Ortholog Locus:PA2291
Sequence Length:1359
Protein Length:452
Start: 3110344
Stop: 3111702
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05679.1|AE004655_1 (AE004655) probable glucose-sensitive porin [Pseudomonas aeruginosa PAO1]
Identities = 449/452 (99%), Positives = 449/452 (99%)
Sequence:
GTGAAATCCCATCTTCTCCGTCCGGCCCTGCGCCCCGTGGCGGGCAGCCTGCTGTCCGCCTCCCTGCTGTGC
AACGCGGCGCACGCCGCCGAGGCGTTCTCGCCGAACTCGAAATGGATGCTCGGCGATTGGGGCGGCAAGCGC
ACCGAGCTGCTGGAGAAGGGCTACGACTTCAAGCTGGAGTACGTCGGCGAGGCGGCGGCCAACCTCGACGGC
GGCTATGACGACGACAAGACCGGGCGCTACACCGACCAGTTCGCCCTGGGCGTGCACATGGACCTGGAGAAG
ATCCTCGGCTGGAAGGCCACCGAGTTCCAGTTCACCGTCACCGAGCGCAATGGCAAGAACCTTTCCAACGAC
CGCATCGGCGACCCGCGTGCCGGGCACATCAGCTCGGTGCAGGAGGTCTGGGGCCGCGGGCAGACCTGGCGG
CTGACCCAGCTGTGGCTCAAGCAGCAGTACTTCGACGGCGCGCTGGACGTGAAATTCGGCCGTTTCGGCGAG
GGCGAGGACTTCAACAGCTTCCCCTGCGATTTCCAGAACCTGGCCTTCTGCGGCTCGCAGGTGGGCAACTGG
GCGGGGAGCATCTGGTACAACTGGCCGGTCAGCCAGTGGGCGTTGCGGGTGAAGTACAACTTCGCGCCGGAC
TGGTACGTGCAGGTCGGCGCCTACGAGCAGAACCCGTCGAACCTGGAGACCGGCAACGGCTTCAAGATGAGC
GGCAGCGGGACCAAGGGCGCGCTGCTGCCGGTGGAGCTGATCTGGCAGCCGAAGGTCGGCGCCGAGCAACTG
CCGGGCGAGTACCGGCTGGGCTACTACTACAGCACGGCGAAGGCCGATGACGTCTACGACGACGTCGACGGC
CAGCCGCAGGGGCTGACCGGCAACGACTTCAAGTCGCGCGGCAGCAAGCATGGCTGGTGGGTGGTGGCGCAG
CAGCAGGTCACTTCGCACAACGGCGATGCTTCGCGCGGGTTGAGCCTGTTCGCCAACCTGACGGTCCACGAC
AAGGCGACCAACGTGGTGGACAACTACCAGCAGCTCGGGGTGGTCTACAAGGGTCCGTTCGATGCGCGGCCG
AAGGACGACATCGGCCTGGGCATCGCACGCATCCATGTCAACGACGATGTGAAGAAGCGCCAGCGCCTGGTG
AACCAGGTGAACGGCATCGACGACTACGACAACCCGCTGTACCAGCCGCTGCAGGACACCGAGTACAACGCC
GAGCTGTACTACGGGGTGCATGTGACCGACTGGCTGACGGTGCGGCCGAACCTGCAATACATCAAGCAGCCG
GGCGGGGTCGACGAGGTCGACAACGCGCTGGTGGCGGGGATCAAGATCCAGACGGTGTTCTAG
Translation:
MKSHLLRPALRPVAGSLLSASLLCNAAHAAEAFSPNSKWMLGDWGGKRTELLEKGYDFKLEYVGEAAANLDG
GYDDDKTGRYTDQFALGVHMDLEKILGWKATEFQFTVTERNGKNLSNDRIGDPRAGHISSVQEVWGRGQTWR
LTQLWLKQQYFDGALDVKFGRFGEGEDFNSFPCDFQNLAFCGSQVGNWAGSIWYNWPVSQWALRVKYNFAPD
WYVQVGAYEQNPSNLETGNGFKMSGSGTKGALLPVELIWQPKVGAEQLPGEYRLGYYYSTAKADDVYDDVDG
QPQGLTGNDFKSRGSKHGWWVVAQQQVTSHNGDASRGLSLFANLTVHDKATNVVDNYQQLGVVYKGPFDARP
KDDIGLGIARIHVNDDVKKRQRLVNQVNGIDDYDNPLYQPLQDTEYNAELYYGVHVTDWLTVRPNLQYIKQP
GGVDEVDNALVAGIKIQTVF*
AnnotationID:914GeneID:905
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:25
Gene Name:
Confidence Code:3
GeneProduct:probable glucose-sensitive porin
Cell Localization:()
Synonyms:PA2291, opbA, oprB2
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3659,pfam04966
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66644.1|AE016292_4 (AE016777) porin B [Pseudomonas putida KT2440]
          Length = 447

 Score =  743 bits (1917), Expect = 0.0
 Identities = 345/446 (77%), Positives = 383/446 (85%), Gaps = 2/446 (0%)

>gb|AAN67067.1|AE016335_7 (AE016779) porin B [Pseudomonas putida KT2440]
          Length = 444

 Score =  653 bits (1684), Expect = 0.0
 Identities = 299/435 (68%), Positives = 350/435 (80%), Gaps = 2/435 (0%)

>gb|AAC37160.1| (L05770) putative porin [Acinetobacter sp. ADP1]
          Length = 439

 Score =  395 bits (1015), Expect = e-109
 Identities = 201/443 (45%), Positives = 275/443 (62%), Gaps = 19/443 (4%)






 
Structural Features:
COG3659, OprB, Carbohydrate-selective porin 
[Cell envelope biogenesis, outer membrane]
CD-Length = 439 residues, 100.0% aligned
Score =  575 bits (1482), Expect = 5e-165
Identities = 312/452 (69%), Positives = 344/452 (76%), Gaps = 13/452 (2%)

pfam04966, OprB, Carbohydrate-selective porin, OprB family.
CD-Length = 355 residues, 100.0% aligned
Score =  400 bits (1028), Expect = 3e-112
Identities = 173/374 (46%), Positives = 225/374 (60%), Gaps = 28/374 (7%)








   
Genomic Context:






   
Comment:


















    ReferenceID:14253




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18955




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32496




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:905_cdd.html


    		




 
URL:

















    ReferenceID:34461




  
Author/Investigator(s):
        Wylie JL, Worobec EA.





  
Title:
        Cloning and nucleotide sequence of the Pseudomonas aeruginosa glucose-selective OprB porin gene and distribution of OprB within the family Pseudomonadaceae.




  
PubMed:
        8125108


MedLine:
        




    
Source:
Eur J Biochem. 1994 Mar 1;220(2):505-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8125108









           
Homologs By Global Alignment 

           

       



     
Gene ID:905
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1609
                           PA2291_tr
                           translation of PA2291
                           452
                           99.33
                           99.33
                           0
                           2437.0
     

     
     
58357
                           gb|AAG05679.1|AE004655_1
                           (AE004655) probable glucose-sensitive porin [Pseudomonas aeruginosa PAO1]
                           452
                           99.11
                           99.33
                           0
                           2434.0
     

     
     
1610
                           PA3186_tr
                           translation of PA3186
                           455
                           96.26
                           96.70
                           4
                           2347.0
     

     
     
58358
                           gb|AAG06574.1|AE004742_10
                           (AE004742) Glucose/carbohydrate outer membrane porin OprB precursor [Pseudomonas aeruginosa PAO1]
                           455
                           96.26
                           96.70
                           4
                           2347.0
     

     
     
58359
                           emb|CAA54387.1|
                           (X77131) porin [Pseudomonas aeruginosa]
                           455
                           96.26
                           96.70
                           4
                           2347.0
     

     






      

          
GC ORFID: 43907How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2291Glimmer Score: 

          
Start: 1843572Stop: 1842214

          
Length: 1359


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2291 translation of PA2291Homolog Bit Score 943.0

          
Other Homologs:  t_PA3186 (ORF: BLASTX 907.0), psyr_15may02_Scaffold3_revised_gene4422 (ORF: BLASTX 697.0), t_PA2291 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAAATCCCATCTTCTCCGTCCGGCCCTGCGCCCCGTGGCGGGCAGCCTGCTGTCCGCCTCCCTGCTGTGC 
AACGCGGCGCACGCCGCCGAGGCGTTCTCGCCGAACTCGAAATGGATGCTCGGCGATTGGGGCGGCAAGCGC 
ACCGAGCTGCTGGAGAAGGGCTACGACTTCAAGCTGGAGTACGTCGGCGAGGCGGCGGCCAACCTCGACGGC 
GGCTATGACGACGACAAGACCGGGCGCTACACCGACCAGTTCGCCCTGGGCGTGCACATGGACCTGGAGAAG 
ATCCTCGGCTGGAAGGCCACCGAGTTCCAGTTCACCGTCACCGAGCGCAATGGCAAGAACCTTTCCAACGAC 
CGCATCGGCGACCCGCGTGCCGGGCACATCAGCTCGGTGCAGGAGGTCTGGGGCCGCGGGCAGACCTGGCGG 
CTGACCCAGCTGTGGCTCAAGCAGCAGTACTTCGACGGCGCGCTGGACGTGAAATTCGGCCGTTTCGGCGAG 
GGCGAGGACTTCAACAGCTTCCCCTGCGATTTCCAGAACCTGGCCTTCTGCGGCTCGCAGGTGGGCAACTGG 
GCGGGGAGCATCTGGTACAACTGGCCGGTCAGCCAGTGGGCGTTGCGGGTGAAGTACAACTTCGCGCCGGAC 
TGGTACGTGCAGGTCGGCGCCTACGAGCAGAACCCGTCGAACCTGGAGACCGGCAACGGCTTCAAGATGAGC 
GGCAGCGGGACCAAGGGCGCGCTGCTGCCGGTGGAGCTGATCTGGCAGCCGAAGGTCGGCGCCGAGCAACTG 
CCGGGCGAGTACCGGCTGGGCTACTACTACAGCACGGCGAAGGCCGATGACGTCTACGACGACGTCGACGGC 
CAGCCGCAGGGGCTGACCGGCAACGACTTCAAGTCGCGCGGCAGCAAGCATGGCTGGTGGGTGGTGGCGCAG 
CAGCAGGTCACTTCGCACAACGGCGATGCTTCGCGCGGGTTGAGCCTGTTCGCCAACCTGACGGTCCACGAC 
AAGGCGACCAACGTGGTGGACAACTACCAGCAGCTCGGGGTGGTCTACAAGGGTCCGTTCGATGCGCGGCCG 
AAGGACGACATCGGCCTGGGCATCGCACGCATCCATGTCAACGACGATGTGAAGAAGCGCCAGCGCCTGGTG 
AACCAGGTGAACGGCATCGACGACTACGACAACCCGCTGTACCAGCCGCTGCAGGACACCGAGTACAACGCC 
GAGCTGTACTACGGGGTGCATGTGACCGACTGGCTGACGGTGCGGCCGAACCTGCAATACATCAAGCAGCCG 
GGCGGGGTCGACGAGGTCGACAACGCGCTGGTGGCGGGGATCAAGATCCAGACGGTGTTCTAG