ViewGene
Locus Name:
GeneID:
Location:

GeneID:899
TrivialName: PA14_58190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58190
PAO1 Ortholog Locus:PA4484
Sequence Length:1446
Protein Length:481
Start: 5179126
Stop: 5180571
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07872.1|AE004862_6 (AE004862) Glu-tRNA(Gln) amidotransferase subunit B [Pseudomonas aeruginosa PAO1]
Identities = 480/481 (99%), Positives = 481/481 (100%)
Sequence:
ATGCAATGGGAAACCGTGATCGGGCTGGAAATCCACGCACAGCTCGCCACCCAATCGAAGATCTTCTCCGGC
AGCTCGACCGCCTTCGGCGCCGCGCCGAATACCCAGGCCAGCCTGGTCGACCTGGCCATGCCGGGCACCCTG
CCGGTGCTAAACGAGGAGGCCGTACGCATGGCCTGCCTGTTCGGCCTGGCGATCGACGCACGGATCGACCGG
CAGAACGTGTTCGCCCGCAAGAACTACTTCTACCCTGACCTGCCGAAGGGCTACCAGACCAGCCAGATGGAC
CATCCCATCGTCGGCAAGGGCCACCTCGACATCACCCTGGAAGACGGCACCACCAAGCGCATCGGCATCACC
CGCGCGCACCTGGAGGAGGACGCCGGCAAGAGCCTGCACGAAGACTTCCAGGGCATGAGCGGCATCGACCTG
AACCGCGCCGGCACGCCGTTGCTGGAGATCGTTTCCGAGCCGGATATCCGCAGCGCCAAGGAAGCCGTGGCC
TACGTCAAGGCGATCCACGCCCTGGTGCGCTACCTGGGCATCTGCGACGGCAACATGGCCGAGGGCTCGCTG
CGCTGCGACTGCAACGTCTCGGTGCGACCGAAGGGCCAGGCCGAATTCGGCACCCGCGCCGAGATCAAGAAC
GTCAACTCCTTCCGCTTCATCGAGAAGGCGATCAACCACGAGATCCAGCGCCAGATCGAGCTGATCGAGGAC
GGCGGCAAGGTGGTCCAGGAAACCCGCCTGTACGACCCGAACAAGGACGAGACCCGCTCCATGCGCGGCAAG
GAAGAAGCCAACGACTACCGTTACTTCCCCTGTCCCGACCTGCTGCCGGTGGTCATCGAACCCGAGTACCTG
GCGAAGCTGCGCGAGCAACTGCCGGAACTGCCGGTGCAGAAGCGCGAACGCTTCGAGAGCCAGTACGGCCTG
TCCGCCTACGATGCCAGCGTGCTGTCCGCCAGCCGCGAGATGGCCGACTACTTCGAGAAGGTCCAGGGTATC
TGCGGCGACGCCAAGCTGGCGGCCAACTGGGTGATGGTCGAGCTGGGCAGCCTGCTCAACAAGGACGGCCTG
GAGATCGAGCAGTCTCCGGTGTCCGCCGAGCAACTGGGCGGCATGATCCTGCGCATCAAGGACAACACCATC
TCCGGCAAGATCGCCAAGATGGTCTTCGAAGCCATGGCCAACGGCGAGGGTTCCGCCGACCAGATCATCGAG
GCCAAGGGCCTGAAGCAGGTCACCGACAGTGGCGCCATCGAGAAGATGCTCGACGAGGTGCTGGCGGCCAAC
GCCGAGCAGGTCGAGCAGTACCGCGCCGCCGACGAGGCCAAGCGCGGCAAGATGTTCGGCTTCTTCGTCGGC
CAGGCGATGAAGGCCTCGAAAGGCAAGGCCAACCCGCAGCAAGTGAACGAGTTGCTGAAGAAAAAGCTCGAA
GCGTGA
Translation:
MQWETVIGLEIHAQLATQSKIFSGSSTAFGAAPNTQASLVDLAMPGTLPVLNEEAVRMACLFGLAIDARIDR
QNVFARKNYFYPDLPKGYQTSQMDHPIVGKGHLDITLEDGTTKRIGITRAHLEEDAGKSLHEDFQGMSGIDL
NRAGTPLLEIVSEPDIRSAKEAVAYVKAIHALVRYLGICDGNMAEGSLRCDCNVSVRPKGQAEFGTRAEIKN
VNSFRFIEKAINHEIQRQIELIEDGGKVVQETRLYDPNKDETRSMRGKEEANDYRYFPCPDLLPVVIEPEYL
AKLREQLPELPVQKRERFESQYGLSAYDASVLSASREMADYFEKVQGICGDAKLAANWVMVELGSLLNKDGL
EIEQSPVSAEQLGGMILRIKDNTISGKIAKMVFEAMANGEGSADQIIEAKGLKQVTDSGAIEKMLDEVLAAN
AEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKKKLEA*
AnnotationID:908GeneID:899
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 14:02:52
Gene Name:gatB
Confidence Code:2
GeneProduct:Glu-tRNA(Gln) amidotransferase subunit B
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0064,KOG2438
Secondary Functional Category(ies):
EC Number:6.3.5.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57924.1| (AE016872) glutamyl-tRNA(Gln) amidotransferase, B subunit
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 481

 Score =  819 bits (2116), Expect = 0.0
 Identities = 409/480 (85%), Positives = 440/480 (91%)

>gb|AAN66555.1|AE016284_3 (AE016777) glutamyl-tRNA(Gln) amidotransferase, B subunit
           [Pseudomonas putida KT2440]
          Length = 481

 Score =  816 bits (2109), Expect = 0.0
 Identities = 411/480 (85%), Positives = 441/480 (91%)

>gb|AAF41732.1| (AE002484) Glu-tRNA(Gln) amidotransferase, subunit B [Neisseria
           meningitidis MC58]
          Length = 476

 Score =  573 bits (1476), Expect = e-162
 Identities = 292/480 (60%), Positives = 362/480 (75%), Gaps = 6/480 (1%)






 
Structural Features:
COG0064, GatB, Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit 
(PET112 homolog) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 483 residues,  99.0% aligned
Score =  698 bits (1803), Expect = 0.0
Identities = 292/482 (60%), Positives = 358/482 (74%), Gaps = 6/482 (1%)

KOG2438, Glutamyl-tRNA amidotransferase subunit B 
[Translation, ribosomal structure and biogenesis]
CD-Length = 505 residues,  95.8% aligned
Score =  470 bits (1210), Expect = 5e-133
Identities = 208/489 (42%), Positives = 291/489 (59%), Gaps = 14/489 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1801




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18943




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31526




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:899_cdd.html


    		




 
URL:

















    ReferenceID:32890




  
Author/Investigator(s):
        Raczniak G, Becker HD, Min B, Soll D.





  
Title:
        A single amidotransferase forms asparaginyl-tRNA and glutaminyl-tRNA in Chlamydia trachomatis.




  
PubMed:
        11585842


MedLine:
        




    
Source:
J Biol Chem. 2001 Dec 7;276(49):45862-7. Epub 2001 Oct 03.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11585842

















    ReferenceID:32891




  
Author/Investigator(s):
        Salazar JC, Zuniga R, Raczniak G, Becker H, Soll D, Orellana O.





  
Title:
        A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans.




  
PubMed:
        11445070


MedLine:
        




    
Source:
FEBS Lett. 2001 Jul 6;500(3):129-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11445070

















    ReferenceID:32892




  
Author/Investigator(s):
        Curnow AW, Hong K, Yuan R, Kim S, Martins O, Winkler W, Henkin TM, Soll D.





  
Title:
        Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.




  
PubMed:
        9342321


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11819-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9342321

















    ReferenceID:32893




  
Author/Investigator(s):
        Nobelmann B, Lengeler JW.





  
Title:
        Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism.




  
PubMed:
        8955298


MedLine:
        




    
Source:
J Bacteriol. 1996 Dec;178(23):6790-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8955298









           
Homologs By Global Alignment 

           

       



     
Gene ID:899
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1599
                           PA4484_tr
                           translation of PA4484
                           481
                           99.79
                           99.99
                           0
                           2445.0
     

     
     
57760
                           gb|AAG07872.1|AE004862_6
                           (AE004862) Glu-tRNA(Gln) amidotransferase subunit B [Pseudomonas aeruginosa PAO1]
                           481
                           99.79
                           99.99
                           0
                           2445.0
     

     
     
57762
                           gb|AAN66555.1|AE016284_3
                           (AE016777) glutamyl-tRNA(Gln) amidotransferase, B subunit [Pseudomonas putida KT2440]
                           481
                           85.44
                           91.68
                           0
                           2109.0
     

     
     
57761
                           gb|AAO57924.1|
                           (AE016872) glutamyl-tRNA(Gln) amidotransferase, B subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           481
                           85.03
                           91.47
                           0
                           2116.0
     

     
     
57765
                           emb|CAD13582.1|
                           (AL646057) PROBABLE GLU-TRNAGLN AMIDOTRANSFERASE SUBUNIT B PROTEIN [Ralstonia solanacearum]
                           492
                           65.04
                           77.03
                           15
                           1571.0
     

     






      

          
GC ORFID: 43901How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4484Glimmer Score: 

          
Start: 4442805Stop: 4444250

          
Length: 1446


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4484 translation of PA4484Homolog Bit Score 946.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5565 (ORF: BLASTX 819.0), t_PA4484 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAATGGGAAACCGTGATCGGGCTGGAAATCCACGCACAGCTCGCCACCCAATCGAAGATCTTCTCCGGC 
AGCTCGACCGCCTTCGGCGCCGCGCCGAATACCCAGGCCAGCCTGGTCGACCTGGCCATGCCGGGCACCCTG 
CCGGTGCTAAACGAGGAGGCCGTACGCATGGCCTGCCTGTTCGGCCTGGCGATCGACGCACGGATCGACCGG 
CAGAACGTGTTCGCCCGCAAGAACTACTTCTACCCTGACCTGCCGAAGGGCTACCAGACCAGCCAGATGGAC 
CATCCCATCGTCGGCAAGGGCCACCTCGACATCACCCTGGAAGACGGCACCACCAAGCGCATCGGCATCACC 
CGCGCGCACCTGGAGGAGGACGCCGGCAAGAGCCTGCACGAAGACTTCCAGGGCATGAGCGGCATCGACCTG 
AACCGCGCCGGCACGCCGTTGCTGGAGATCGTTTCCGAGCCGGATATCCGCAGCGCCAAGGAAGCCGTGGCC 
TACGTCAAGGCGATCCACGCCCTGGTGCGCTACCTGGGCATCTGCGACGGCAACATGGCCGAGGGCTCGCTG 
CGCTGCGACTGCAACGTCTCGGTGCGACCGAAGGGCCAGGCCGAATTCGGCACCCGCGCCGAGATCAAGAAC 
GTCAACTCCTTCCGCTTCATCGAGAAGGCGATCAACCACGAGATCCAGCGCCAGATCGAGCTGATCGAGGAC 
GGCGGCAAGGTGGTCCAGGAAACCCGCCTGTACGACCCGAACAAGGACGAGACCCGCTCCATGCGCGGCAAG 
GAAGAAGCCAACGACTACCGTTACTTCCCCTGTCCCGACCTGCTGCCGGTGGTCATCGAACCCGAGTACCTG 
GCGAAGCTGCGCGAGCAACTGCCGGAACTGCCGGTGCAGAAGCGCGAACGCTTCGAGAGCCAGTACGGCCTG 
TCCGCCTACGATGCCAGCGTGCTGTCCGCCAGCCGCGAGATGGCCGACTACTTCGAGAAGGTCCAGGGTATC 
TGCGGCGACGCCAAGCTGGCGGCCAACTGGGTGATGGTCGAGCTGGGCAGCCTGCTCAACAAGGACGGCCTG 
GAGATCGAGCAGTCTCCGGTGTCCGCCGAGCAACTGGGCGGCATGATCCTGCGCATCAAGGACAACACCATC 
TCCGGCAAGATCGCCAAGATGGTCTTCGAAGCCATGGCCAACGGCGAGGGTTCCGCCGACCAGATCATCGAG 
GCCAAGGGCCTGAAGCAGGTCACCGACAGTGGCGCCATCGAGAAGATGCTCGACGAGGTGCTGGCGGCCAAC 
GCCGAGCAGGTCGAGCAGTACCGCGCCGCCGACGAGGCCAAGCGCGGCAAGATGTTCGGCTTCTTCGTCGGC 
CAGGCGATGAAGGCCTCGAAAGGCAAGGCCAACCCGCAGCAAGTGAACGAGTTGCTGAAGAAAAAGCTCGAA 
GCGTGA