ViewGene
Locus Name:
GeneID:
Location:

GeneID:890
TrivialName: PA14_71970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71970
PAO1 Ortholog Locus:PA5452
Sequence Length:1440
Protein Length:479
Start: 6416025
Stop: 6414586
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08837.1|AE004958_7 (AE004958) phosphomannose isomerase/GDP-mannose WbpW [Pseudomonas aeruginosa PAO1]
Identities = 478/479 (99%), Positives = 478/479 (99%)
Sequence:
ATGCTGATTCCCGTGGTGCTTTCCGGCGGCGCCGGTACCCGCCTGTGGCCGGTGTCGCGTGAAGGACAGCCG
AAGCCGTTCATGCGCCTGCCCGATGGCCAGACCCTGCTGGGCAAGACCTACCGACGTGCCGCCGGGCTCCTG
GCCGGGCATGGCGAAATCGTCACGGTGACCAACCGCGAGCATTACTTCCAGAGCAAGGACCAGTTCCAGGCA
GCCCGCCTGGGGCGGCACCGAGGACATTTCATCCTCGAACCGACCGGGCGCAACACCGCTCCGGCGATTGCC
GTCGCCGCCCTGGCGCTGCAAGCCGAACATGGCGACGCGGCGGTACTGGTGGTGATGCCGGCGGATCACCTG
ATCCGCAACGAGGAGGCCTTCCGCGAGGCGGTAGGGCATGCCGCGCGTCTCGCCGTCGCCGGGCACCTGGTT
ACCTTCGGCGTGGTGCCGGACGCAGCGGAAACCGGTTTCGGCTATATCGAGCTGGGCGACCGGCTGGACGAG
CAGGGGGCGGCCAAGGTGCGCCGCTTCGTCGAGAAACCCGATGAGGAAACCGCCCGTCGCTACGTAGAAAGT
GGCGGTTTCCTGTGGAATTCCGGGATGTTCTGTTTCACGGCCTCGACCCTGGTCGACGAGCTGGCGCAGCAT
GCCCCGGCCCTGCTGGAGCAGGCCAGGGCCTGCCTGGCCGCCAGTGCGGCGGTGAAGATGGCCGATGGCATC
CAGCATGAGCTGGCAGGCGAAGCCTTCGCCGCGCTGCCGGACATTTCCATCGACTATGCGCTGATGGAGCGC
TCCGCGCGGGTCGCTGTGGTGCCGGCGGCCTTCGACTGGAGCGATATCGGTTCCTGGGGCGCGATGAGCGCG
CTGCTCGACGCGGACGCAGAGGGCAATCGCGGCAGCGGCGACACCCTGTTCGTCGACACCCGTAACACCTTC
GTGCAGAGCGACGGGCGCCTGGTCGCCACGGTCGGGGTCGACGACCTGGTCGTGGTGGATACTTCCGATGCG
CTGCTGATCGCCCGCGCCGACCGGGTCCAGGAGGTGCGCAGGGTGGTGCAGCGGTTGAAGGACGAGCGGCAC
GAGGCCTATCGGCTGCATCGGACCGTCAACCGTCCCTGGGGCAGCTATACCGTCCTCGAGGAAGGGCCGCGC
TTCAAGATCAAGCGAATCGTGGTCAGGCCGGGAGAGCGCTTGTCGCTGCAGATGCACCATCACCGCAGCGAG
CACTGGATCGTGGTGCAAGGCATGGCCAGGGTCACCAATGGCGATGGCGCGCGGCTGGTCAACAGCAACGAG
TCGACCTACATTCCCGCGGGCCACCGGCATCGCCTGGAAAACCCGGGGGTCATCGACCTGGTGATGATCGAG
GTGCAGAGCGGCGAATACCTGGGAGAGGACGACATCGTCCGCTTCGAGGACCAGTACGGCAGGGTGGTCTGA
Translation:
MLIPVVLSGGAGTRLWPVSREGQPKPFMRLPDGQTLLGKTYRRAAGLLAGHGEIVTVTNREHYFQSKDQFQA
ARLGRHRGHFILEPTGRNTAPAIAVAALALQAEHGDAAVLVVMPADHLIRNEEAFREAVGHAARLAVAGHLV
TFGVVPDAAETGFGYIELGDRLDEQGAAKVRRFVEKPDEETARRYVESGGFLWNSGMFCFTASTLVDELAQH
APALLEQARACLAASAAVKMADGIQHELAGEAFAALPDISIDYALMERSARVAVVPAAFDWSDIGSWGAMSA
LLDADAEGNRGSGDTLFVDTRNTFVQSDGRLVATVGVDDLVVVDTSDALLIARADRVQEVRRVVQRLKDERH
EAYRLHRTVNRPWGSYTVLEEGPRFKIKRIVVRPGERLSLQMHHHRSEHWIVVQGMARVTNGDGARLVNSNE
STYIPAGHRHRLENPGVIDLVMIEVQSGEYLGEDDIVRFEDQYGRVV*
AnnotationID:899GeneID:890
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 21:09:06
Gene Name:wbpW
Confidence Code:1
GeneProduct:GDP-mannose pyrophosphorylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:phosphomannose isomerase
Functional Category Confidence Code:5
COGs:COG0836,pfam01050,pfam00483,COG0662
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN33545.1|AE014534_7 (AE014534) mannose-1-phosphate
           guanylyltransferase/mannose-6-phosphate isomerase
           [Brucella suis 1330]
          Length = 471

 Score =  552 bits (1423), Expect = e-156
 Identities = 279/476 (58%), Positives = 353/476 (74%), Gaps = 8/476 (1%)

>gb|AAO57005.1| (AE016868) mannose-1-phosphate
           guanylyltransferase/mannose-6-phosphate isomerase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 485

 Score =  550 bits (1416), Expect = e-155
 Identities = 285/478 (59%), Positives = 344/478 (71%), Gaps = 2/478 (0%)









 
Structural Features:
COG0836, {ManC}, Mannose-1-phosphate guanylyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 333 residues, 100.0% aligned
Score =  451 bits (1161), Expect = 1e-127
Identities = 166/341 (48%), Positives = 228/341 (66%), Gaps = 8/341 (2%)

pfam01050, MannoseP_isomer, Mannose-6-phosphate isomerase.
CD-Length = 167 residues, 100.0% aligned
Score =  258 bits (662), Expect = 8e-70 
Identities = 108/167 (64%), Positives = 135/167 (80%)

pfam00483, NTP_transferase, Nucleotidyl transferase. 
CD-Length = 246 residues,  97.2% aligned
Score =  165 bits (420), Expect = 8e-42
Identities = 85/290 (29%), Positives = 129/290 (44%), Gaps = 51/290 (17%)

COG0662, {ManC}, Mannose-6-phosphate isomerase 
[Carbohydrate transport and metabolism]
CD-Length = 127 residues, 100.0% aligned
Score =  153 bits (388), Expect = 3e-38
Identities = 71/127 (55%), Positives = 87/127 (68%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1783




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18925




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34644




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:890_cdd.html


    		




 
URL:

















    ReferenceID:35621




  
Author/Investigator(s):
        Rocchetta HL, Pacan JC, Lam JS.





  
Title:
        Synthesis of the A-band polysaccharide sugar D-rhamnose requires Rmd and WbpW: identification of multiple AlgA homologues, WbpW and ORF488, in Pseudomonas aeruginosa.




  
PubMed:
        9781879


MedLine:
        




    
Source:
Mol Microbiol. 1998 Sep;29(6):1419-34.

Erratum in:
    * Mol Microbiol 1999 Jan;31(1):397-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9781879









           
Homologs By Global Alignment 

           

       



     
Gene ID:890
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1583
                           PA5452_tr
                           translation of PA5452
                           479
                           99.79
                           99.79
                           0
                           2456.0
     

     
     
57215
                           gb|AAG08837.1|AE004958_7
                           (AE004958) phosphomannose isomerase/GDP-mannose WbpW [Pseudomonas aeruginosa PAO1]
                           479
                           99.79
                           99.79
                           0
                           2456.0
     

     
     
57216
                           gb|AAC72283.1|
                           (AF009956) WbpW [Pseudomonas aeruginosa]
                           479
                           99.16
                           99.16
                           0
                           2443.0
     

     
     
1584
                           PA2232_tr
                           translation of PA2232
                           491
                           58.65
                           71.28
                           15
                           1448.0
     

     
     
57217
                           gb|AAG05620.1|AE004649_11
                           (AE004649) probable phosphomannose isomerase/GDP-mannose pyrophosphorylase [Pseudomonas aeruginosa PAO1]
                           491
                           58.65
                           71.28
                           15
                           1448.0
     

     






      

          
GC ORFID: 43892How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5452Glimmer Score: 

          
Start: 5679714Stop: 5678275

          
Length: 1440


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5452 translation of PA5452Homolog Bit Score 950.0

          
Other Homologs:  psyr_15may02_Scaffold11_revised_gene1912 (ORF: BLASTX 554.0), psyr_15may02_Scaffold3_revised_gene4287 (ORF: BLASTX 432.0), psyr_15may02_Scaffold3_revised_gene5002 (ORF: BLASTX 418.0), t_PA3551 (ORF: BLASTX 417.0), t_PA5452 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGATTCCCGTGGTGCTTTCCGGCGGCGCCGGTACCCGCCTGTGGCCGGTGTCGCGTGAAGGACAGCCG 
AAGCCGTTCATGCGCCTGCCCGATGGCCAGACCCTGCTGGGCAAGACCTACCGACGTGCCGCCGGGCTCCTG 
GCCGGGCATGGCGAAATCGTCACGGTGACCAACCGCGAGCATTACTTCCAGAGCAAGGACCAGTTCCAGGCA 
GCCCGCCTGGGGCGGCACCGAGGACATTTCATCCTCGAACCGACCGGGCGCAACACCGCTCCGGCGATTGCC 
GTCGCCGCCCTGGCGCTGCAAGCCGAACATGGCGACGCGGCGGTACTGGTGGTGATGCCGGCGGATCACCTG 
ATCCGCAACGAGGAGGCCTTCCGCGAGGCGGTAGGGCATGCCGCGCGTCTCGCCGTCGCCGGGCACCTGGTT 
ACCTTCGGCGTGGTGCCGGACGCAGCGGAAACCGGTTTCGGCTATATCGAGCTGGGCGACCGGCTGGACGAG 
CAGGGGGCGGCCAAGGTGCGCCGCTTCGTCGAGAAACCCGATGAGGAAACCGCCCGTCGCTACGTAGAAAGT 
GGCGGTTTCCTGTGGAATTCCGGGATGTTCTGTTTCACGGCCTCGACCCTGGTCGACGAGCTGGCGCAGCAT 
GCCCCGGCCCTGCTGGAGCAGGCCAGGGCCTGCCTGGCCGCCAGTGCGGCGGTGAAGATGGCCGATGGCATC 
CAGCATGAGCTGGCAGGCGAAGCCTTCGCCGCGCTGCCGGACATTTCCATCGACTATGCGCTGATGGAGCGC 
TCCGCGCGGGTCGCTGTGGTGCCGGCGGCCTTCGACTGGAGCGATATCGGTTCCTGGGGCGCGATGAGCGCG 
CTGCTCGACGCGGACGCAGAGGGCAATCGCGGCAGCGGCGACACCCTGTTCGTCGACACCCGTAACACCTTC 
GTGCAGAGCGACGGGCGCCTGGTCGCCACGGTCGGGGTCGACGACCTGGTCGTGGTGGATACTTCCGATGCG 
CTGCTGATCGCCCGCGCCGACCGGGTCCAGGAGGTGCGCAGGGTGGTGCAGCGGTTGAAGGACGAGCGGCAC 
GAGGCCTATCGGCTGCATCGGACCGTCAACCGTCCCTGGGGCAGCTATACCGTCCTCGAGGAAGGGCCGCGC 
TTCAAGATCAAGCGAATCGTGGTCAGGCCGGGAGAGCGCTTGTCGCTGCAGATGCACCATCACCGCAGCGAG 
CACTGGATCGTGGTGCAAGGCATGGCCAGGGTCACCAATGGCGATGGCGCGCGGCTGGTCAACAGCAACGAG 
TCGACCTACATTCCCGCGGGCCACCGGCATCGCCTGGAAAACCCGGGGGTCATCGACCTGGTGATGATCGAG 
GTGCAGAGCGGCGAATACCTGGGAGAGGACGACATCGTCCGCTTCGAGGACCAGTACGGCAGGGTGGTCTGA