ViewGene
Locus Name:
GeneID:
Location:

GeneID:888
TrivialName: PA14_57330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57330
PAO1 Ortholog Locus:PA4411
Sequence Length:1443
Protein Length:480
Start: 5108633
Stop: 5107191
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07799.1|AE004856_10 (AE004856) UDP-N-acetylmuramate--alanine ligase [Pseudomonas aeruginosa PAO1]
Identities = 480/480 (100%), Positives = 480/480 (100%)
Sequence:
GTGGTTAAAGAACCGAATGGCGTCACCCGGACCATGCGCCGTATCCGCCGCATCCATTTCGTCGGCATCGGC
GGCGCCGGCATGTGCGGGATCGCCGAAGTGCTGCTGAACCTCGGCTACGAGGTATCCGGCTCGGACCTCAAG
GCCTCGGCGGTGACCGAGCGCCTGGAGAAGTTCGGCGCGCAGATCTTCATCGGCCACCAGGCGGAAAACGCC
GACGGTGCCGACGTGCTGGTGGTGTCCAGTGCCATCAACCGGGCCAACCCGGAAGTGGCCTCGGCCCTGGAA
CGGCGGATTCCGGTGGTGCCGCGTGCGGAGATGCTCGCCGAACTGATGCGCTACCGGCACGGCATCGCGGTA
GCCGGCACCCACGGCAAGACCACCACTACCAGCCTGATCGCCTCGGTGTTCGCCGCCGGCGGCCTGGACCCG
ACCTTCGTCATCGGCGGTCGGCTGAACGCCGCCGGGACCAACGCCCAGCTTGGCGCCAGCCGCTACCTGGTG
GCCGAGGCCGACGAGAGCGACGCCAGCTTCCTGCACCTGCAACCGATGGTCGCGGTGGTCACCAATATCGAC
GCCGACCACATGGCGACCTACGGCGGCGACTTCAACAAGCTGAAGAAGACCTTCGTCGAGTTCCTCCACAAC
CTGCCGTTCTACGGACTGGCAGTGATGTGCGTGGATGACCCGGTGGTGCGCGAGATCCTCCCGCAGATCGCC
CGCCCGACCGTGACCTACGGCCTCAGCGAAGACGCCGACGTGCGCGCGATCAACATCCGCCAGGAAGGCATG
CGCACCTGGTTCACCGTGTTGCGCCCGGAGCGCGAGCCGCTGGACGTCTCGGTGAACATGCCCGGCCTGCAC
AACGTGCTGAATTCCCTGGCGACCATCGTCATCGCTACCGACGAGGGCATCTCCGACGAAGCCATCGTCCAG
GGGCTGTCCGGCTTCCAGGGCGTAGGCCGGCGCTTCCAGGTCTACGGCGAGCTGCAGGTCGAGGGTGGCAGC
GTGATGCTGGTGGACGACTACGGCCACCATCCGCGCGAAGTCGCCGCGGTGATCAAGGCGATCCGTGGCGGT
TGGCCGGAGCGTCGCCTGGTGATGGTCTACCAGCCGCATCGCTATACCCGTACCCGCGACCTGTACGAAGAC
TTCGTGCAGGTGCTGGGCGAAGCCAACGTGCTGCTGTTGATGGAGGTCTATCCGGCCGGCGAAGAGCCGATC
CCGGGAGCCGACAGCCGCCAGCTGTGCCACAGCATCCGCCAGCGCGGTCAGCTCGACCCGATCTACTTCGAG
CGCGACGCCGACCTGGCGCCGCTGGTCAAGCCGCTGCTGCGCGCTGGCGACATCCTGCTTTGCCAGGGCGCT
GGCGATGTCGGCGGCCTGGCCCCGCAACTGATCAAGAACCCGCTGTTCGCCGGCAAGGGAGGGAAGGGCGCA
TGA
Translation:
MVKEPNGVTRTMRRIRRIHFVGIGGAGMCGIAEVLLNLGYEVSGSDLKASAVTERLEKFGAQIFIGHQAENA
DGADVLVVSSAINRANPEVASALERRIPVVPRAEMLAELMRYRHGIAVAGTHGKTTTTSLIASVFAAGGLDP
TFVIGGRLNAAGTNAQLGASRYLVAEADESDASFLHLQPMVAVVTNIDADHMATYGGDFNKLKKTFVEFLHN
LPFYGLAVMCVDDPVVREILPQIARPTVTYGLSEDADVRAINIRQEGMRTWFTVLRPEREPLDVSVNMPGLH
NVLNSLATIVIATDEGISDEAIVQGLSGFQGVGRRFQVYGELQVEGGSVMLVDDYGHHPREVAAVIKAIRGG
WPERRLVMVYQPHRYTRTRDLYEDFVQVLGEANVLLLMEVYPAGEEPIPGADSRQLCHSIRQRGQLDPIYFE
RDADLAPLVKPLLRAGDILLCQGAGDVGGLAPQLIKNPLFAGKGGKGA*
AnnotationID:897GeneID:888
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 17:05:14
Gene Name:murC
Confidence Code:2
GeneProduct:UDP-N-acetylmuramate-alanine ligase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0773,pfam01225
Secondary Functional Category(ies):
EC Number:6.3.2.8
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66961.1|AE016324_11 (AE016779) UDP-N-acetylmuramate--alanine ligase [Pseudomonas putida
           KT2440]
          Length = 482

 Score =  835 bits (2158), Expect = 0.0
 Identities = 415/463 (89%), Positives = 442/463 (95%)


>gb|AAO57856.1| (AE016871) UDP-N-acetylmuramate--alanine ligase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 486

 Score =  833 bits (2153), Expect = 0.0
 Identities = 410/463 (88%), Positives = 438/463 (94%)






 
Structural Features:
COG0773, MurC, UDP-N-acetylmuramate-alanine ligase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 459 residues,  98.7% aligned
Score =  602 bits (1554), Expect = 6e-173
Identities = 248/461 (53%), Positives = 322/461 (69%), Gaps = 11/461 (2%)

pfam01225, Mur_ligase, Mur ligase family, catalytic domain.
CD-Length = 267 residues,  96.6% aligned
Score =  154 bits (391), Expect = 3e-38
Identities = 86/265 (32%), Positives = 116/265 (43%), Gaps = 20/265 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14251




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18921




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:29532




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:888_cdd.html


    		




 
URL:

















    ReferenceID:32797




  
Author/Investigator(s):
        El Zoeiby A, Sanschagrin F, Darveau A, Brisson JR, Levesque RC.





  
Title:
        Identification of novel inhibitors of Pseudomonas aeruginosa MurC enzyme derived from phage-displayed peptide libraries.




  
PubMed:
        12615853


MedLine:
        




    
Source:
J Antimicrob Chemother. 2003 Mar;51(3):531-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12615853

















    ReferenceID:32798




  
Author/Investigator(s):
        Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M.





  
Title:
        The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes.




  
PubMed:
        11281713


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281713

















    ReferenceID:32799




  
Author/Investigator(s):
        El Zoeiby A, Sanschagrin F, Lamoureux J, Darveau A, Levesque RC.





  
Title:
        Cloning, over-expression and purification of Pseudomonas aeruginosa murC encoding uridine diphosphate N-acetylmuramate: L-alanine ligase.




  
PubMed:
        10675598


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2000 Feb 15;183(2):281-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10675598









           
Homologs By Global Alignment 

           

       



     
Gene ID:888
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1581
                           PA4411_tr
                           translation of PA4411
                           480
                           99.99
                           99.99
                           0
                           2456.0
     

     
     
57137
                           gb|AAG07799.1|AE004856_10
                           (AE004856) UDP-N-acetylmuramate--alanine ligase [Pseudomonas aeruginosa PAO1]
                           480
                           99.79
                           99.99
                           0
                           2453.0
     

     
     
57135
                           gb|AAG48251.1|AF306766_1
                           (AF306766) UDP-N-acetylmuramate:L-alanine ligase MurC [Pseudomonas aeruginosa]
                           487
                           98.56
                           98.56
                           7
                           2456.0
     

     
     
57136
                           gb|AAF14862.1|AF110740_1
                           (AF110740) UDP-N-acetylmuramate:L-alanine ligase MurC [Pseudomonas aeruginosa]
                           487
                           98.56
                           98.56
                           7
                           2456.0
     

     
     
57138
                           gb|AAN66961.1|AE016324_11
                           (AE016779) UDP-N-acetylmuramate--alanine ligase [Pseudomonas putida KT2440]
                           487
                           85.83
                           91.78
                           12
                           2150.0
     

     






      

          
GC ORFID: 43890How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4411Glimmer Score: 

          
Start: 4372311Stop: 4370869

          
Length: 1443


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4411 translation of PA4411Homolog Bit Score 950.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1129 (ORF: BLASTX 833.0), t_PA4411 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGTTAAAGAACCGAATGGCGTCACCCGGACCATGCGCCGTATCCGCCGCATCCATTTCGTCGGCATCGGC 
GGCGCCGGCATGTGCGGGATCGCCGAAGTGCTGCTGAACCTCGGCTACGAGGTATCCGGCTCGGACCTCAAG 
GCCTCGGCGGTGACCGAGCGCCTGGAGAAGTTCGGCGCGCAGATCTTCATCGGCCACCAGGCGGAAAACGCC 
GACGGTGCCGACGTGCTGGTGGTGTCCAGTGCCATCAACCGGGCCAACCCGGAAGTGGCCTCGGCCCTGGAA 
CGGCGGATTCCGGTGGTGCCGCGTGCGGAGATGCTCGCCGAACTGATGCGCTACCGGCACGGCATCGCGGTA 
GCCGGCACCCACGGCAAGACCACCACTACCAGCCTGATCGCCTCGGTGTTCGCCGCCGGCGGCCTGGACCCG 
ACCTTCGTCATCGGCGGTCGGCTGAACGCCGCCGGGACCAACGCCCAGCTTGGCGCCAGCCGCTACCTGGTG 
GCCGAGGCCGACGAGAGCGACGCCAGCTTCCTGCACCTGCAACCGATGGTCGCGGTGGTCACCAATATCGAC 
GCCGACCACATGGCGACCTACGGCGGCGACTTCAACAAGCTGAAGAAGACCTTCGTCGAGTTCCTCCACAAC 
CTGCCGTTCTACGGACTGGCAGTGATGTGCGTGGATGACCCGGTGGTGCGCGAGATCCTCCCGCAGATCGCC 
CGCCCGACCGTGACCTACGGCCTCAGCGAAGACGCCGACGTGCGCGCGATCAACATCCGCCAGGAAGGCATG 
CGCACCTGGTTCACCGTGTTGCGCCCGGAGCGCGAGCCGCTGGACGTCTCGGTGAACATGCCCGGCCTGCAC 
AACGTGCTGAATTCCCTGGCGACCATCGTCATCGCTACCGACGAGGGCATCTCCGACGAAGCCATCGTCCAG 
GGGCTGTCCGGCTTCCAGGGCGTAGGCCGGCGCTTCCAGGTCTACGGCGAGCTGCAGGTCGAGGGTGGCAGC 
GTGATGCTGGTGGACGACTACGGCCACCATCCGCGCGAAGTCGCCGCGGTGATCAAGGCGATCCGTGGCGGT 
TGGCCGGAGCGTCGCCTGGTGATGGTCTACCAGCCGCATCGCTATACCCGTACCCGCGACCTGTACGAAGAC 
TTCGTGCAGGTGCTGGGCGAAGCCAACGTGCTGCTGTTGATGGAGGTCTATCCGGCCGGCGAAGAGCCGATC 
CCGGGAGCCGACAGCCGCCAGCTGTGCCACAGCATCCGCCAGCGCGGTCAGCTCGACCCGATCTACTTCGAG 
CGCGACGCCGACCTGGCGCCGCTGGTCAAGCCGCTGCTGCGCGCTGGCGACATCCTGCTTTGCCAGGGCGCT 
GGCGATGTCGGCGGCCTGGCCCCGCAACTGATCAAGAACCCGCTGTTCGCCGGCAAGGGAGGGAAGGGCGCA 
TGA