ViewGene
Locus Name:
GeneID:
Location:

GeneID:885
TrivialName: PA14_03430
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03430
PAO1 Ortholog Locus:PA0265
Sequence Length:1452
Protein Length:483
Start: 313663
Stop: 315114
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03654.1|AE004464_7 (AE004464) succinate-semialdehyde dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 482/483 (99%), Positives = 482/483 (99%)
Sequence:
ATGCAACTCAAAGATGCCAAGCTGTTCCGTCAACAAGCCTACGTCGATGGCGCCTGGGTGGATGCCGACAAC
GGCCAGACCATCAAGGTGAACAACCCGGCCACCGGCGAGATCATCGGCAGCGTGCCGAAGATGGGCGCCGCC
GAGACCCGCCGCGCCATCGAGGCTGCCGACAAGGCCCTGCCGGCCTGGCGTGCGCTGACCGCCAAGGAGCGC
GCCAACAAGCTGCGCCGCTGGTTCGACCTGATGATCGAGAACCAGGACGACCTGGCCCGCCTGATGACCATC
GAGCAGGGCAAGCCGCTGGCCGAGGCCAAGGGCGAGATCGCCTACGCCGCCTCCTTCCTCGAATGGTTCGGC
GAAGAAGCCAAGCGCATCTACGGCGACACCATTCCCGGCCACCAGCCGGACAAGCGCATCATCGTGATCAAG
CAGCCGATCGGTGTGACCGCGGCCATCACGCCGTGGAACTTCCCCTCGGCGATGATCACCCGCAAGGCCGGC
CCGGCCCTGGCCGCCGGCTGCACCATGGTGCTCAAGCCTGCTTCGCAGACCCCGTATTCCGCCCTGGCCCTG
GCCGAGCTGGCAGAGCGCGCCGGCATTCCGAAGGGCGTGTTCAGCGTGGTCACCGGTAGCGCCGGCGAAGTC
GGCGGCGAGCTGACCAGCAACCCGATCGTGCGCAAGCTGACCTTCACCGGTTCCACCGAAATCGGCCGCCAA
CTGATGGCCGAGTGCGCCCAGGACATCAAGAAGGTGTCCCTGGAGCTGGGCGGCAACGCGCCGTTCATCGTG
TTCGACGACGCCGACCTGGACGCCGCCGTCGAGGGCGCGCTGATTTCCAAGTACCGCAACAACGGCCAGACC
TGCGTCTGCGCCAACCGCCTGTACGTGCAGGACGGCGTGTACGACGCCTTCGTCGACAAGCTGAAGGCCGCC
GTGGCCAAGCTGAACATCGGCAACGGCCTGGAAGCCGGCGTGACCACCGGTCCGCTGATCGACGCCAAGGCC
GTGACCAAGGTCGAGGAACACATCGCCGACGCGGTCTCCAAGGGCGCCAAGGTGGTTTCCGGCGGCAAGCCG
CACGCCCTCGGCGGCACCTTCTTCGAGCCGACCATCCTGGTCGACGTGCCGAAGAACGCCCTGGTGTCCAAG
GACGAGACCTTCGGCCCGCTGGCGCCGGTGTTCCGCTTCAAGGACGAGGCCGAGGTCATCGCCATGTCCAAC
GACACCGAGTTCGGCCTGGCGTCCTACTTCTACGCCCGCGACCTGGCCCGCGTGTTCCGCGTCGCCGAGCAA
CTGGAGTACGGCATGGTCGGCATCAACACCGGCCTGATCTCCAACGAAGTGGCGCCGTTCGGCGGCATCAAG
GCCTCGGGCCTGGGCCGCGAAGGCTCCAAGTACGGGATCGAGGACTACCTCGAGATCAAGTACCTCTGCCTG
GGCGGTATCTGA
Translation:
MQLKDAKLFRQQAYVDGAWVDADNGQTIKVNNPATGEIIGSVPKMGAAETRRAIEAADKALPAWRALTAKER
ANKLRRWFDLMIENQDDLARLMTIEQGKPLAEAKGEIAYAASFLEWFGEEAKRIYGDTIPGHQPDKRIIVIK
QPIGVTAAITPWNFPSAMITRKAGPALAAGCTMVLKPASQTPYSALALAELAERAGIPKGVFSVVTGSAGEV
GGELTSNPIVRKLTFTGSTEIGRQLMAECAQDIKKVSLELGGNAPFIVFDDADLDAAVEGALISKYRNNGQT
CVCANRLYVQDGVYDAFVDKLKAAVAKLNIGNGLEAGVTTGPLIDAKAVTKVEEHIADAVSKGAKVVSGGKP
HALGGTFFEPTILVDVPKNALVSKDETFGPLAPVFRFKDEAEVIAMSNDTEFGLASYFYARDLARVFRVAEQ
LEYGMVGINTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYLCLGGI*
AnnotationID:894GeneID:885
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 05:05:36
Gene Name:gabD
Confidence Code:2
GeneProduct: succinate-semialdehyde dehydrogenase, NADP-dependent activity
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00171,COG1012
Secondary Functional Category(ies):energy metabolism
EC Number:1.2.1.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65845.1|AE016213_1 (AE016774) succinate-semialdehyde dehydrogenase [Pseudomonas putida
           KT2440]
          Length = 480

 Score =  850 bits (2195), Expect = 0.0
 Identities = 424/480 (88%), Positives = 450/480 (93%), Gaps = 2/480 (0%)

>gb|AAN81661.1|AE016765_63 (AE016765) Succinate-semialdehyde dehydrogenase [NADP+]
           [Escherichia coli CFT073]
          Length = 482

 Score =  798 bits (2061), Expect = 0.0
 Identities = 391/481 (81%), Positives = 438/481 (91%)

>gb|AAL21676.1| (AE008827) NADP-dependent succinate-semialdehyde dehydrogenase I
           [Salmonella typhimurium LT2]
          Length = 482

 Score =  791 bits (2042), Expect = 0.0
 Identities = 386/481 (80%), Positives = 435/481 (90%)






 
Structural Features:
pfam00171, Aldedh, Aldehyde dehydrogenase family.
CD-Length = 464 residues,  99.4% aligned
Score =  548 bits (1413), Expect = 7e-157
Identities = 222/461 (48%), Positives = 295/461 (63%)

COG1012, PutA, NAD-dependent aldehyde dehydrogenases 
[Energy production and conversion]
CD-Length = 472 residues,  98.9% aligned
Score =  538 bits (1387), Expect = 6e-154
Identities = 237/472 (50%), Positives = 323/472 (68%), Gaps = 7/472 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1773




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18915




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33617




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:885_cdd.html


    		




 
URL:

















    ReferenceID:35448




  
Author/Investigator(s):
        Niegemann E, Schulz A, Bartsch K.





  
Title:
        Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene.




  
PubMed:
        8297211


MedLine:
        




    
Source:
Arch Microbiol. 1993;160(6):454-60.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8297211

















    ReferenceID:35449




  
Author/Investigator(s):
        Bartsch K, von Johnn-Marteville A, Schulz A.





  
Title:
        Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD).




  
PubMed:
        2254272


MedLine:
        




    
Source:
J Bacteriol. 1990 Dec;172(12):7035-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2254272

















    ReferenceID:35450




  
Author/Investigator(s):
        Lutke-Eversloh T, Steinbuchel A.





  
Title:
        Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha.




  
PubMed:
        10564790


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 Dec 1;181(1):63-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10564790









           
Homologs By Global Alignment 

           

       



     
Gene ID:885
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1577
                           PA0265_tr
                           translation of PA0265
                           483
                           99.79
                           99.79
                           0
                           2459.0
     

     
     
57012
                           gb|AAG03654.1|AE004464_7
                           (AE004464) succinate-semialdehyde dehydrogenase [Pseudomonas aeruginosa PAO1]
                           483
                           99.79
                           99.79
                           0
                           2459.0
     

     
     
57013
                           gb|AAN65845.1|AE016213_1
                           (AE016774) succinate-semialdehyde dehydrogenase [Pseudomonas putida KT2440]
                           483
                           87.78
                           93.16
                           3
                           2194.0
     

     
     
57014
                           gb|AAO53845.1|
                           (AE016857) succinate-semialdehyde dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           483
                           84.47
                           93.37
                           3
                           2142.0
     

     
     
57016
                           gb|AAG57768.1|AE005495_3
                           (AE005495) succinate-semialdehyde dehydrogenase, NADP-dependent activity [Escherichia coli O157:H7 EDL933]
                           483
                           81.15
                           90.68
                           1
                           2056.0
     

     






      

          
GC ORFID: 43887How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0265Glimmer Score: 

          
Start: 6115004Stop: 6116455

          
Length: 1452


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0265 translation of PA0265Homolog Bit Score 951.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4041 (ORF: BLASTX 829.0), psyr_15may02_Scaffold2_revised_gene3988 (ORF: BLASTX 599.0), psyr_15may02_Scaffold1_revised_gene1348 (ORF: BLASTX 374.0), psyr_15may02_Scaffold1_revised_gene155 (ORF: BLASTX 365.0), t_PA0265 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAACTCAAAGATGCCAAGCTGTTCCGTCAACAAGCCTACGTCGATGGCGCCTGGGTGGATGCCGACAAC 
GGCCAGACCATCAAGGTGAACAACCCGGCCACCGGCGAGATCATCGGCAGCGTGCCGAAGATGGGCGCCGCC 
GAGACCCGCCGCGCCATCGAGGCTGCCGACAAGGCCCTGCCGGCCTGGCGTGCGCTGACCGCCAAGGAGCGC 
GCCAACAAGCTGCGCCGCTGGTTCGACCTGATGATCGAGAACCAGGACGACCTGGCCCGCCTGATGACCATC 
GAGCAGGGCAAGCCGCTGGCCGAGGCCAAGGGCGAGATCGCCTACGCCGCCTCCTTCCTCGAATGGTTCGGC 
GAAGAAGCCAAGCGCATCTACGGCGACACCATTCCCGGCCACCAGCCGGACAAGCGCATCATCGTGATCAAG 
CAGCCGATCGGTGTGACCGCGGCCATCACGCCGTGGAACTTCCCCTCGGCGATGATCACCCGCAAGGCCGGC 
CCGGCCCTGGCCGCCGGCTGCACCATGGTGCTCAAGCCTGCTTCGCAGACCCCGTATTCCGCCCTGGCCCTG 
GCCGAGCTGGCAGAGCGCGCCGGCATTCCGAAGGGCGTGTTCAGCGTGGTCACCGGTAGCGCCGGCGAAGTC 
GGCGGCGAGCTGACCAGCAACCCGATCGTGCGCAAGCTGACCTTCACCGGTTCCACCGAAATCGGCCGCCAA 
CTGATGGCCGAGTGCGCCCAGGACATCAAGAAGGTGTCCCTGGAGCTGGGCGGCAACGCGCCGTTCATCGTG 
TTCGACGACGCCGACCTGGACGCCGCCGTCGAGGGCGCGCTGATTTCCAAGTACCGCAACAACGGCCAGACC 
TGCGTCTGCGCCAACCGCCTGTACGTGCAGGACGGCGTGTACGACGCCTTCGTCGACAAGCTGAAGGCCGCC 
GTGGCCAAGCTGAACATCGGCAACGGCCTGGAAGCCGGCGTGACCACCGGTCCGCTGATCGACGCCAAGGCC 
GTGACCAAGGTCGAGGAACACATCGCCGACGCGGTCTCCAAGGGCGCCAAGGTGGTTTCCGGCGGCAAGCCG 
CACGCCCTCGGCGGCACCTTCTTCGAGCCGACCATCCTGGTCGACGTGCCGAAGAACGCCCTGGTGTCCAAG 
GACGAGACCTTCGGCCCGCTGGCGCCGGTGTTCCGCTTCAAGGACGAGGCCGAGGTCATCGCCATGTCCAAC 
GACACCGAGTTCGGCCTGGCGTCCTACTTCTACGCCCGCGACCTGGCCCGCGTGTTCCGCGTCGCCGAGCAA 
CTGGAGTACGGCATGGTCGGCATCAACACCGGCCTGATCTCCAACGAAGTGGCGCCGTTCGGCGGCATCAAG 
GCCTCGGGCCTGGGCCGCGAAGGCTCCAAGTACGGGATCGAGGACTACCTCGAGATCAAGTACCTCTGCCTG 
GGCGGTATCTGA