ViewGene
Locus Name:
GeneID:
Location:

GeneID:88
TrivialName: PA14_07770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_07770
PAO1 Ortholog Locus:PA0595
Sequence Length:2775
Protein Length:924
Start: 669348
Stop: 666574
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03984.1|AE004495_8 (AE004495) organic solvent tolerance protein OstA precursor [Pseudomonas aeruginosa PAO1]
Identities = 922/924 (99%), Positives = 924/924 (100%)
Sequence:
ATGGCAGTGAAATCCCTCGTGTTTCGAAGAAAGTTTCCCCTGCTGGTCACCGGCAGCCTGCTGGCTCTGCAG
CCGGTCGCAGCATTGACTGTACAGGCCGCCGATCAGTTCGACTGCAAGGTATCGGCCACCGGCGGCTGGGAT
TGCTCCCCGCTGCAGAACGCCAATGCCAACCTGCCACCGCGCCCGGCGCATACCGCGACCTCGGTCAGCACC
GCCGCGGCGGGCAGCAGCGTGTCCGGCAGCGGAGGCGAAACCGTCGAGGCAGAGCCGACCCAGCGCCTGGTC
ACCGAGTCCGGCGGTCGCGCGCTGAGATCCCGCAGTGCGGACTACTCGCACCTCGACTGGATTCCTCGGGAA
AAACTGACCGCCGCCCAGTTGGCGGAAATCGGCCCCTACTGCGGCGGCTCCTACATCGAGCCGGTGCGTCCG
GGCATGGATGACGGCGCGCCCAGCGACGAGAGCCCGACCTACGTATCCGCCAAGGCTTCGCGCTACGAGCAG
GAAAAACAGATCGCCACCCTCGCCGGTGACGTGGTCCTGCGCCAGGGCAGCATGCAGGTGGAAGGCGACGAG
GCCAACCTGCACCAGCTGGAAAACCGCGGCGAACTGGTCGGCAACGTCAAGCTGCGCGACAAGGGCATGCTG
GTGGTCGGCGACCACGCCCAGGTCCAGCTGGACAACGGCGAGGCCCAGGTCGACAACGCCGAGTACGTGATC
CACAAGGCCCATGCCCGCGGCAGCGCGCTCTACGCCAAGCGCAGCGAGAACGCCATCATCATGCTCAAGGAT
GGCACCTACACCCGCTGCGAACCGAGCAGCAACGCCTGGACCCTGAAGGGCAACAACGTCAAGCTGAACCCG
GCCACCGGCTTCGGCACCGCGACCAACGCGACCCTGCGGGTCAAAGATTTCCCGGTGTTCTACACCCCGTAC
ATCTATTTCCCGATCGACGACCGCCGCCAGTCCGGTTTCCTGCCGCCGAGCTTCAGCAGCACCAGCGACACC
GGCTTCACCCTGGTCACCCCGTACTACTTCAACCTGGCGCCGAACTACGACGCCACGTTGTACCCGCGCTAC
ATGGCCAAGCGCGGCATGATGCTGGAAGGCGAGTTCCGCTACCTGACCCACAGCAGCGAAGGTATCGTCAAC
GCTGCCTACCTTAACGACAAGGACGATCACCGCGAAGGTTTCCCGGACTACAGCAAGGACCGCTGGCTGTAC
GGTCTCAAGAACACCACCGGCCTCGATTCGCGCTGGCTGGCCGAGGTCGACTACACGCGGATCAGTGATCCC
TATTACTTCCAGGATCTGGATACTGACCTTGGCGTAGGCAGCACTACGTACGTCAACCAACGGGGCACTCTG
ACCTATCGCGGGGACACCTTTACTGGCCGTTTGAATGCGCAGGCGTACCAGTTGGCGACCACCACCGATGTA
ACCCCGTACGACCGGCTGCCACAGATTACCTTCGACGGTTTCCTGCCCTACAACCCAGGTGGAATGCAATTC
ACCTATGGCACCGAGTTCGTTCGATTCGATCGAGATCTAGACGAAAATATCTATTTCAATGACGACGGCAGC
ATCCGCGGCAAACGCCCAGATGCGTCACTTCAAGGCCTTGCCCGAGCAACGGGAGACCGTATGCATCTGGAG
CCGGGCATGAGTTTGCCAATGACGCGCAGCTGGGGTTATGTGACACCCACATTGAAATATTTGTATACAAAG
TACGATCTAGACTTGGATAGCCAGGGCAAGACCGACCTGAATAAAAGAGATGAGTCCTTTGACAGCAATCAG
GACCGTTCGCTCCCTTTGGTTAAGGTCGACAGCGGCCTGTATTTCGACCGAGACACCACCTTTGCCGGCACA
CCATTCCGCCAAACTCTCGAGCCGCGTGCCATGTACTTGTACGTTCCATATAAGGACCAAGACAGCCTGCCG
GTCTTCGATACCAGTGAACCGTCCTTCAGTTACGACTCTCTGTGGCGCGAGAACCGCTTTACCGGTAAGGAT
CGTATCGGCGATGCCAACCAGTTGTCCCTGGGCGTCACCAGTCGTTTCATCGAAGAGAACGGTTTCGAACGC
GCCAGCATCAGTGCCGGCCAGATTTACTACTTCCGTGATCGTCGCGTCCAACTACCAGGCCTAACCGAGAAG
GATCTGAAGCGACTCAACCTAGATCCCAGCGGCTTGGACAATGACAGCTGGCGCTCTCCCTACGCCTTTGCC
GGCCAGTACCGTTTCAACCGCGACTGGCGCATCAACTCCGACTTCAATTGGAACCCCAACACCAGTCGTACT
GAGTCCGGTAGCGCCATCTTCCACTACCAGCCCGAAGTCGATCCGGGCAAGGTGGTCAACGTTGGCTATCGC
TATCGTGCAGATGCTCGGCGCTTTGATTCCTCCCGCGGCACATTCCGATATGGCAACGAAGACGACATCATC
AAACAGCACGACTTCTCGGTCATCTGGCCGCTGGTGCCGCAGTGGTCCGTGCTCGCCCGCTGGCAGTACGAC
TACAACAAGAACCGCACACTCGAGGCCTTCGGCGGCTTCGAATACGACAGCTGCTGCTGGAAGCTGCGCCTG
ATCAACCGCTACTGGCTGGATGTGGACGATGACGCCTTCCTCGTGCAGAGCGAGAAGGCCGACCGCGGCATC
TTCCTGCAGATCGTCCTGAAGGGCCTCGGCGGCATCGTAGGCAACAAGACGGAAATGTTCCTCGACAAAGGC
ATCCAAGGTTATCGTCAACGTGAAGATCAAGCTATGTAA
Translation:
MAVKSLVFRRKFPLLVTGSLLALQPVAALTVQAADQFDCKVSATGGWDCSPLQNANANLPPRPAHTATSVST
AAAGSSVSGSGGETVEAEPTQRLVTESGGRALRSRSADYSHLDWIPREKLTAAQLAEIGPYCGGSYIEPVRP
GMDDGAPSDESPTYVSAKASRYEQEKQIATLAGDVVLRQGSMQVEGDEANLHQLENRGELVGNVKLRDKGML
VVGDHAQVQLDNGEAQVDNAEYVIHKAHARGSALYAKRSENAIIMLKDGTYTRCEPSSNAWTLKGNNVKLNP
ATGFGTATNATLRVKDFPVFYTPYIYFPIDDRRQSGFLPPSFSSTSDTGFTLVTPYYFNLAPNYDATLYPRY
MAKRGMMLEGEFRYLTHSSEGIVNAAYLNDKDDHREGFPDYSKDRWLYGLKNTTGLDSRWLAEVDYTRISDP
YYFQDLDTDLGVGSTTYVNQRGTLTYRGDTFTGRLNAQAYQLATTTDVTPYDRLPQITFDGFLPYNPGGMQF
TYGTEFVRFDRDLDENIYFNDDGSIRGKRPDASLQGLARATGDRMHLEPGMSLPMTRSWGYVTPTLKYLYTK
YDLDLDSQGKTDLNKRDESFDSNQDRSLPLVKVDSGLYFDRDTTFAGTPFRQTLEPRAMYLYVPYKDQDSLP
VFDTSEPSFSYDSLWRENRFTGKDRIGDANQLSLGVTSRFIEENGFERASISAGQIYYFRDRRVQLPGLTEK
DLKRLNLDPSGLDNDSWRSPYAFAGQYRFNRDWRINSDFNWNPNTSRTESGSAIFHYQPEVDPGKVVNVGYR
YRADARRFDSSRGTFRYGNEDDIIKQHDFSVIWPLVPQWSVLARWQYDYNKNRTLEAFGGFEYDSCCWKLRL
INRYWLDVDDDAFLVQSEKADRGIFLQIVLKGLGGIVGNKTEMFLDKGIQGYRQREDQAM*
AnnotationID:98GeneID:88
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 11:11:59
Gene Name:ostA
Confidence Code:2
GeneProduct:organic solvent tolerance protein OstA precursor
Cell Localization:()
Synonyms:imp
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1452,pfam04453
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54096.1| (AE016857) organic solvent tolerance protein, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 905

 Score = 1189 bits (3076), Expect = 0.0
 Identities = 576/919 (62%), Positives = 708/919 (77%), Gaps = 33/919 (3%)








 
Structural Features:
COG1452, Imp, Organic solvent tolerance protein OstA 
[Cell envelope biogenesis, outer membrane]
CD-Length = 784 residues,  99.7% aligned
Score =  619 bits (1597), Expect = 5e-178
Identities = 280/826 (33%), Positives = 393/826 (47%), Gaps = 57/826 (6%)

pfam04453, OstA_C, Organic solvent tolerance protein. 
CD-Length = 393 residues, 100.0% aligned
Score =  309 bits (793), Expect = 1e-84
Identities = 141/446 (31%), Positives = 205/446 (45%), Gaps = 58/446 (13%)






   
Genomic Context:






   
Comment:


















    ReferenceID:179




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17321




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33886




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:88_cdd.html


    		




 
URL:

















    ReferenceID:35265




  
Author/Investigator(s):
        Aono R, Negishi T, Nakajima H.





  
Title:
        Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli.




  
PubMed:
        7811102


MedLine:
        




    
Source:
Appl Environ Microbiol. 1994 Dec;60(12):4624-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7811102

















    ReferenceID:35266




  
Author/Investigator(s):
        Aono R, Negishi T, Aibe K, Inoue A, Horikoshi K.





  
Title:
        Mapping of organic solvent tolerance gene ostA in Escherichia coli K-12.




  
PubMed:
        7765247


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1994 Jul;58(7):1231-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7765247

















    ReferenceID:35267




  
Author/Investigator(s):
        Ohtsu I, Kakuda N, Tsukagoshi N, Dokyu N, Takagi H, Wachi M, Aono R.





  
Title:
         
Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli.




  
PubMed:
        14981317


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 2004 Feb;68(2):458-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14981317

















    ReferenceID:35268




  
Author/Investigator(s):
        Abe S, Okutsu T, Nakajima H, Kakuda N, Ohtsu I, Aono R.





  
Title:
        n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane.




  
PubMed:
        12724388


MedLine:
        




    
Source:
Microbiology. 2003 May;149(Pt 5):1265-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12724388









           
Homologs By Global Alignment 

           

       



     
Gene ID:88
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
167
                           PA0595_tr
                           translation of PA0595
                           924
                           99.78
                           99.99
                           0
                           4907.0
     

     
     
14084
                           gb|AAG03984.1|AE004495_8
                           (AE004495) organic solvent tolerance protein OstA precursor [Pseudomonas aeruginosa PAO1]
                           924
                           99.78
                           99.99
                           0
                           4907.0
     

     
     
14085
                           gb|AAO54096.1|
                           (AE016857) organic solvent tolerance protein, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           942
                           61.35
                           75.37
                           55
                           3107.0
     

     






      

          
GC ORFID: 43090How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0595Glimmer Score: 

          
Start: 6470691Stop: 6467917

          
Length: 2775


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0595 translation of PA0595Homolog Bit Score 1894.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6752 (ORF: BLASTX 1233.0), t_PA0595 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCAGTGAAATCCCTCGTGTTTCGAAGAAAGTTTCCCCTGCTGGTCACCGGCAGCCTGCTGGCTCTGCAG 
CCGGTCGCAGCATTGACTGTACAGGCCGCCGATCAGTTCGACTGCAAGGTATCGGCCACCGGCGGCTGGGAT 
TGCTCCCCGCTGCAGAACGCCAATGCCAACCTGCCACCGCGCCCGGCGCATACCGCGACCTCGGTCAGCACC 
GCCGCGGCGGGCAGCAGCGTGTCCGGCAGCGGAGGCGAAACCGTCGAGGCAGAGCCGACCCAGCGCCTGGTC 
ACCGAGTCCGGCGGTCGCGCGCTGAGATCCCGCAGTGCGGACTACTCGCACCTCGACTGGATTCCTCGGGAA 
AAACTGACCGCCGCCCAGTTGGCGGAAATCGGCCCCTACTGCGGCGGCTCCTACATCGAGCCGGTGCGTCCG 
GGCATGGATGACGGCGCGCCCAGCGACGAGAGCCCGACCTACGTATCCGCCAAGGCTTCGCGCTACGAGCAG 
GAAAAACAGATCGCCACCCTCGCCGGTGACGTGGTCCTGCGCCAGGGCAGCATGCAGGTGGAAGGCGACGAG 
GCCAACCTGCACCAGCTGGAAAACCGCGGCGAACTGGTCGGCAACGTCAAGCTGCGCGACAAGGGCATGCTG 
GTGGTCGGCGACCACGCCCAGGTCCAGCTGGACAACGGCGAGGCCCAGGTCGACAACGCCGAGTACGTGATC 
CACAAGGCCCATGCCCGCGGCAGCGCGCTCTACGCCAAGCGCAGCGAGAACGCCATCATCATGCTCAAGGAT 
GGCACCTACACCCGCTGCGAACCGAGCAGCAACGCCTGGACCCTGAAGGGCAACAACGTCAAGCTGAACCCG 
GCCACCGGCTTCGGCACCGCGACCAACGCGACCCTGCGGGTCAAAGATTTCCCGGTGTTCTACACCCCGTAC 
ATCTATTTCCCGATCGACGACCGCCGCCAGTCCGGTTTCCTGCCGCCGAGCTTCAGCAGCACCAGCGACACC 
GGCTTCACCCTGGTCACCCCGTACTACTTCAACCTGGCGCCGAACTACGACGCCACGTTGTACCCGCGCTAC 
ATGGCCAAGCGCGGCATGATGCTGGAAGGCGAGTTCCGCTACCTGACCCACAGCAGCGAAGGTATCGTCAAC 
GCTGCCTACCTTAACGACAAGGACGATCACCGCGAAGGTTTCCCGGACTACAGCAAGGACCGCTGGCTGTAC 
GGTCTCAAGAACACCACCGGCCTCGATTCGCGCTGGCTGGCCGAGGTCGACTACACGCGGATCAGTGATCCC 
TATTACTTCCAGGATCTGGATACTGACCTTGGCGTAGGCAGCACTACGTACGTCAACCAACGGGGCACTCTG 
ACCTATCGCGGGGACACCTTTACTGGCCGTTTGAATGCGCAGGCGTACCAGTTGGCGACCACCACCGATGTA 
ACCCCGTACGACCGGCTGCCACAGATTACCTTCGACGGTTTCCTGCCCTACAACCCAGGTGGAATGCAATTC 
ACCTATGGCACCGAGTTCGTTCGATTCGATCGAGATCTAGACGAAAATATCTATTTCAATGACGACGGCAGC 
ATCCGCGGCAAACGCCCAGATGCGTCACTTCAAGGCCTTGCCCGAGCAACGGGAGACCGTATGCATCTGGAG 
CCGGGCATGAGTTTGCCAATGACGCGCAGCTGGGGTTATGTGACACCCACATTGAAATATTTGTATACAAAG 
TACGATCTAGACTTGGATAGCCAGGGCAAGACCGACCTGAATAAAAGAGATGAGTCCTTTGACAGCAATCAG 
GACCGTTCGCTCCCTTTGGTTAAGGTCGACAGCGGCCTGTATTTCGACCGAGACACCACCTTTGCCGGCACA 
CCATTCCGCCAAACTCTCGAGCCGCGTGCCATGTACTTGTACGTTCCATATAAGGACCAAGACAGCCTGCCG 
GTCTTCGATACCAGTGAACCGTCCTTCAGTTACGACTCTCTGTGGCGCGAGAACCGCTTTACCGGTAAGGAT 
CGTATCGGCGATGCCAACCAGTTGTCCCTGGGCGTCACCAGTCGTTTCATCGAAGAGAACGGTTTCGAACGC 
GCCAGCATCAGTGCCGGCCAGATTTACTACTTCCGTGATCGTCGCGTCCAACTACCAGGCCTAACCGAGAAG 
GATCTGAAGCGACTCAACCTAGATCCCAGCGGCTTGGACAATGACAGCTGGCGCTCTCCCTACGCCTTTGCC 
GGCCAGTACCGTTTCAACCGCGACTGGCGCATCAACTCCGACTTCAATTGGAACCCCAACACCAGTCGTACT 
GAGTCCGGTAGCGCCATCTTCCACTACCAGCCCGAAGTCGATCCGGGCAAGGTGGTCAACGTTGGCTATCGC 
TATCGTGCAGATGCTCGGCGCTTTGATTCCTCCCGCGGCACATTCCGATATGGCAACGAAGACGACATCATC 
AAACAGCACGACTTCTCGGTCATCTGGCCGCTGGTGCCGCAGTGGTCCGTGCTCGCCCGCTGGCAGTACGAC 
TACAACAAGAACCGCACACTCGAGGCCTTCGGCGGCTTCGAATACGACAGCTGCTGCTGGAAGCTGCGCCTG 
ATCAACCGCTACTGGCTGGATGTGGACGATGACGCCTTCCTCGTGCAGAGCGAGAAGGCCGACCGCGGCATC 
TTCCTGCAGATCGTCCTGAAGGGCCTCGGCGGCATCGTAGGCAACAAGACGGAAATGTTCCTCGACAAAGGC 
ATCCAAGGTTATCGTCAACGTGAAGATCAAGCTATGTAA