ViewGene
Locus Name:
GeneID:
Location:

GeneID:877
TrivialName: PA14_57410
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57410
PAO1 Ortholog Locus:PA4417
Sequence Length:1464
Protein Length:487
Start: 5116162
Stop: 5114699
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07805.1|AE004856_16 (AE004856) UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase [Pseudomonas aeruginosa PAO1]
Identities = 486/487 (99%), Positives = 486/487 (99%)
Sequence:
ATGCCTATGAGCCTGAACCAACTGTTTCCCCAGGCCGAGCGCGATCTGCTGATCCGCGAGCTGACCCTGGAT
AGCCGCGGCGTTCGTCCGGGCGACCTGTTCCTGGCGGTGCCGGGCGGGCGCCAGGATGGTCGTGCGCACATC
GCCGATGCCCTGGCCAAGGGCGCGGCTGCCGTGGCTTACGAGGCGGAAGGCGCCGGAGAGCTGCCGCCCAGC
GATGCGCCGCTGATCGCGGTGAAGGGGCTGGCCGCGCAACTGTCGGCGGTCGCCGGGCGTTTCTACGGCGAG
CCGAGCCGCGGGCTGGACCTGATCGGCGTCACCGGCACCAACGGCAAGACCAGCGTCAGCCAACTGGTGGCC
CAGGCCCTGGATCTGCTCGGCGAGCGCTGCGGCATCGTCGGCACCCTCGGCACCGGTTTCTACGGCGCCCTG
GAGAGCGGCCGGCACACCACGCCGGACCCGCTCGCGGTGCAAGCCACGCTGGCCACGCTGAAGCAGGCCGGC
GCCCGCGCGGTAGCGATGGAAGTGTCTTCCCACGGCCTCGACCAGGGCCGCGTGGCGGCGCTCGGTTTCGAT
ATCGCGGTGTTCACCAATCTGTCCCGCGACCACCTCGACTATCACGGTTCGATGGAAGCCTATGCCGCCGCC
AAGGCCAAGCTGTTCGCCTGGCCGGGCCTGCGCTGCCGGGTGATCAACCTGGACGACGATTTCGGCCGTCGA
CTGGCCGGCGAGGAACAGGACTCGGAGTTGATCACCTACAGCCTCACCGACAGCTCGGCGTTCCTCTATTGC
CGCGAAGCGCGCTTCGGCGACGCCGGCATCGAGGCGGCACTGGTCACTCCGCACGGCGAGGGCCTGCTGCGC
AGCCCGTTGCTCGGCCGCTTCAACCTGAGCAACCTGCTGGCGGCGGTCGGTGCGCTGCTTGGCCTGGGCTAT
CCCCTGGGCGATATCCTCCGCACCTTGCCGCAACTGCAGGGGCCGGTCGGCCGCATGCAGCGCCTGGGAGGC
GGCGACAAGCCGCTGGTGGTGGTGGACTACGCGCATACTCCCGACGCCCTGGAAAAAGTCCTGGAGGCCCTG
CGTCCGCACGCGGCCGCGCGCCTGCTGTGCCTGTTCGGCTGCGGTGGCGATCGCGATGCCGGCAAGCGTCCG
TTGATGGCTGCGATCGCCGAACGCCTGGCGGATGGGGTGCTGGTCACCGACGACAACCCGCGCACCGAGGCC
AGTGCGGCGATCATCGCCGATATCCGCAAAGGCTTCGCTGCCGCTGACAAGGTTACCTTCCTGCCGTCGCGC
GGTGAGGCGATCGCCCATCTGATCGCTTCCGCTGCGGTGGATGACGTGGTGCTCCTGGCTGGCAAGGGTCAC
GAGGATTATCAGGAGATCGACGGCGTACGCCATCCGTTCTCCGACATCGAGCAGGCCGAGCGCGCCCTGGCC
GCCTGGGAGGTGCCGCATGCTTGA
Translation:
MPMSLNQLFPQAERDLLIRELTLDSRGVRPGDLFLAVPGGRQDGRAHIADALAKGAAAVAYEAEGAGELPPS
DAPLIAVKGLAAQLSAVAGRFYGEPSRGLDLIGVTGTNGKTSVSQLVAQALDLLGERCGIVGTLGTGFYGAL
ESGRHTTPDPLAVQATLATLKQAGARAVAMEVSSHGLDQGRVAALGFDIAVFTNLSRDHLDYHGSMEAYAAA
KAKLFAWPGLRCRVINLDDDFGRRLAGEEQDSELITYSLTDSSAFLYCREARFGDAGIEAALVTPHGEGLLR
SPLLGRFNLSNLLAAVGALLGLGYPLGDILRTLPQLQGPVGRMQRLGGGDKPLVVVDYAHTPDALEKVLEAL
RPHAAARLLCLFGCGGDRDAGKRPLMAAIAERLADGVLVTDDNPRTEASAAIIADIRKGFAAADKVTFLPSR
GEAIAHLIASAAVDDVVLLAGKGHEDYQEIDGVRHPFSDIEQAERALAAWEVPHA*
AnnotationID:886GeneID:877
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 18:06:00
Gene Name:murE
Confidence Code:2
GeneProduct:UDP-N-acetylmuramyl tripeptide synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2, 6-diaminopimelate ligase
Functional Category Confidence Code:5
COGs:COG0769,COG0770,pfam01225
Secondary Functional Category(ies):
EC Number:6.3.2.13
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66955.1|AE016324_5 (AE016779) UDP-N-acetylmuramoylalanyl-D-glutamate-2,
           6-diaminopimelate ligase [Pseudomonas putida KT2440]
          Length = 496

 Score =  720 bits (1858), Expect = 0.0
 Identities = 366/495 (73%), Positives = 409/495 (82%), Gaps = 8/495 (1%)

>emb|CAA58873.1| (X84053) UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-
           diaminopimelate ligase [Pseudomonas aeruginosa]
          Length = 325

 Score =  620 bits (1599), Expect = e-177
 Identities = 317/325 (97%), Positives = 318/325 (97%)






 
Structural Features:
COG0769, MurE, UDP-N-acetylmuramyl tripeptide synthase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 475 residues,  98.5% aligned
Score =  480 bits (1238), Expect = 3e-136
Identities = 223/471 (47%), Positives = 270/471 (57%), Gaps = 7/471 (1%)

COG0770, MurF, UDP-N-acetylmuramyl pentapeptide synthase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 451 residues,  96.5% aligned
Score =  143 bits (362), Expect = 1e-34
Identities = 120/471 (25%), Positives = 196/471 (41%), Gaps = 54/471 (11%)

pfam01225, Mur_ligase, Mur ligase family, catalytic domain. 
CD-Length = 267 residues,  97.8% aligned
Score =  140 bits (353), Expect = 9e-34
Identities = 90/282 (31%), Positives = 118/282 (41%), Gaps = 25/282 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1757




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18899




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31458




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:877_cdd.html


    		




 
URL:

















    ReferenceID:32803




  
Author/Investigator(s):
        Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M.





  
Title:
        The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes.




  
PubMed:
        11281713


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281713

















    ReferenceID:32804




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173

















    ReferenceID:32805




  
Author/Investigator(s):
        Liao X, Hancock RE.





  
Title:
        Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3.




  
PubMed:
        7486937


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1995 Aug;39(8):1871-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7486937









           
Homologs By Global Alignment 

           

       



     
Gene ID:877
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1569
                           PA4417_tr
                           translation of PA4417
                           487
                           99.79
                           99.79
                           0
                           2463.0
     

     
     
56897
                           gb|AAG07805.1|AE004856_16
                           (AE004856) UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase [Pseudomonas aeruginosa PAO1]
                           487
                           99.79
                           99.79
                           0
                           2463.0
     

     
     
56898
                           gb|AAG45235.1|
                           (AY008276) MurE [Pseudomonas aeruginosa]
                           495
                           93.13
                           93.93
                           16
                           2304.0
     

     
     
56899
                           gb|AAO57862.1|
                           (AE016871) UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase [Pseudomonas syringae pv. tomato str. DC3000]
                           487
                           77.20
                           85.01
                           0
                           1924.0
     

     
     
56900
                           gb|AAN66955.1|AE016324_5
                           (AE016779) UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase [Pseudomonas putida KT2440]
                           496
                           73.79
                           82.45
                           9
                           1863.0
     

     






      

          
GC ORFID: 43879How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4417Glimmer Score: 

          
Start: 4379840Stop: 4378377

          
Length: 1464


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4417 translation of PA4417Homolog Bit Score 953.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1123 (ORF: BLASTX 211.0), t_PA4417 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTATGAGCCTGAACCAACTGTTTCCCCAGGCCGAGCGCGATCTGCTGATCCGCGAGCTGACCCTGGAT 
AGCCGCGGCGTTCGTCCGGGCGACCTGTTCCTGGCGGTGCCGGGCGGGCGCCAGGATGGTCGTGCGCACATC 
GCCGATGCCCTGGCCAAGGGCGCGGCTGCCGTGGCTTACGAGGCGGAAGGCGCCGGAGAGCTGCCGCCCAGC 
GATGCGCCGCTGATCGCGGTGAAGGGGCTGGCCGCGCAACTGTCGGCGGTCGCCGGGCGTTTCTACGGCGAG 
CCGAGCCGCGGGCTGGACCTGATCGGCGTCACCGGCACCAACGGCAAGACCAGCGTCAGCCAACTGGTGGCC 
CAGGCCCTGGATCTGCTCGGCGAGCGCTGCGGCATCGTCGGCACCCTCGGCACCGGTTTCTACGGCGCCCTG 
GAGAGCGGCCGGCACACCACGCCGGACCCGCTCGCGGTGCAAGCCACGCTGGCCACGCTGAAGCAGGCCGGC 
GCCCGCGCGGTAGCGATGGAAGTGTCTTCCCACGGCCTCGACCAGGGCCGCGTGGCGGCGCTCGGTTTCGAT 
ATCGCGGTGTTCACCAATCTGTCCCGCGACCACCTCGACTATCACGGTTCGATGGAAGCCTATGCCGCCGCC 
AAGGCCAAGCTGTTCGCCTGGCCGGGCCTGCGCTGCCGGGTGATCAACCTGGACGACGATTTCGGCCGTCGA 
CTGGCCGGCGAGGAACAGGACTCGGAGTTGATCACCTACAGCCTCACCGACAGCTCGGCGTTCCTCTATTGC 
CGCGAAGCGCGCTTCGGCGACGCCGGCATCGAGGCGGCACTGGTCACTCCGCACGGCGAGGGCCTGCTGCGC 
AGCCCGTTGCTCGGCCGCTTCAACCTGAGCAACCTGCTGGCGGCGGTCGGTGCGCTGCTTGGCCTGGGCTAT 
CCCCTGGGCGATATCCTCCGCACCTTGCCGCAACTGCAGGGGCCGGTCGGCCGCATGCAGCGCCTGGGAGGC 
GGCGACAAGCCGCTGGTGGTGGTGGACTACGCGCATACTCCCGACGCCCTGGAAAAAGTCCTGGAGGCCCTG 
CGTCCGCACGCGGCCGCGCGCCTGCTGTGCCTGTTCGGCTGCGGTGGCGATCGCGATGCCGGCAAGCGTCCG 
TTGATGGCTGCGATCGCCGAACGCCTGGCGGATGGGGTGCTGGTCACCGACGACAACCCGCGCACCGAGGCC 
AGTGCGGCGATCATCGCCGATATCCGCAAAGGCTTCGCTGCCGCTGACAAGGTTACCTTCCTGCCGTCGCGC 
GGTGAGGCGATCGCCCATCTGATCGCTTCCGCTGCGGTGGATGACGTGGTGCTCCTGGCTGGCAAGGGTCAC 
GAGGATTATCAGGAGATCGACGGCGTACGCCATCCGTTCTCCGACATCGAGCAGGCCGAGCGCGCCCTGGCC 
GCCTGGGAGGTGCCGCATGCTTGA