ViewGene
Locus Name:
GeneID:
Location:

GeneID:872
TrivialName: PA14_58180
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58180
PAO1 Ortholog Locus:PA4483
Sequence Length:1455
Protein Length:484
Start: 5177565
Stop: 5179019
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07871.1|AE004862_5 (AE004862) Glu-tRNA(Gln) amidotransferase subunit A [Pseudomonas aeruginosa PAO1]
Identities = 482/484 (99%), Positives = 482/484 (99%)
Sequence:
ATGCTGCATCAATTGACCCTCGCCGAGATCGCCCGCGCCCTCGCCGACAAGCAATTTTCCGCCGAAGAGCTG
ACCCGCACCCTGCTCGGTCGCATCCGGCAGCTGGATCCCCAGCTCAACAGCTTCATCAGCATCACCGACGAC
CTGGCCATCGCCCAGGCCAAGGCAGCCGACGAACGGCGCGCCAACGGCGAGAACGGCGCCCTGCTCGGCGCG
CCGATCGCCCACAAGGACCTGTTCTGCACCCAGGGCGTACGCACCAGCTGCGGTTCGAAGATGCTCGACAAC
TTCGTCTCGCCCTACGACGCCACCGTGGTCGAGAAGCTCGCCGCCGCCGGCGCCGTTACCCTCGGCAAGCTG
AACATGGACGAATTCGCCATGGGCTCGTCGAACCAGTCCAGCCACTACGGCGCGGTGAACAACCCCTGGAGC
CTCGACCGCGTGCCTGGCGGCTCCTCCGGCGGCTCCGCCGCGGCAGTCGCCGCGCGCCTGCTGCCGGCCGCC
ACCGGCACCGATACCGGCGGCTCGATCCGCCAGCCGGCGGCGCTGACCAACCTCACCGGGATCAAGCCGACC
TACGGCCGGGTTTCCCGCTGGGGCATGATCGCCTACGCCTCCAGCCTCGACCAGGGCGGCCCGCTGGCGCGC
ACCGCCGAGGACTGCGCGCTGATGCTGGGGGTGATGGCCGGATTCGATCCGAAGGACTCGACCAGCGTCGAA
CAGCCGGTGGACGACTACCTGGCCGCCCTGCAGAAGCCGCTGAGCGGCCTGCGCATCGGCCTGCCGCGGGAA
TACTTCGGCGCCGGCCTCGACAGCCGCATCGCCGACGCGGTGCTGGCCGTGGTCGAGGAGCTGAAGACGCTC
GGCGCCACGGTGAAGGACATTTCCCTGCCGAACATGCAGCACGCCATACCGGCCTACTACGTGATCGCGCCG
GCCGAGGCGTCCTCCAACCTGTCGCGCTTCGACGGCGTGCGCTATGGCTATCGTTGCGACGCCCCGCAGAAC
CTGGAAGACCTGTACAAGCGCTCGCGCGCGGAAGGCTTCGGCAGCGAAGTGAAGAACCGCATCATGGTCGGC
ACCTACGCACTGTCGGCCGGCTACTACGATGCCTATTACCTGCAGGCTCAGAAGATTCGCCGGCTGATCAAG
AACGACTTCGTCAGCGCCTTTGCCGAAGTGGACGTGATCCTCGGCCCGACCACGCCGAACCCGGCCTGGAAG
ATCGGCGAGAAGAACGACGACCCGGTTTCCCAGTACCTGGAAGACATCTACACCATCACCGCCAACCTCGCC
GGCCTGCCGGGGCTGTCCATGCCCGCCGGCTTCGTCGACGGCCTGCCGGTGGGTGTCCAGTTGCTCGCGCCC
TACTTCCAGGAAGGTCGCCTGCTCAACGTCGCGCACCAGTACCAGCAGGTCAGCGACTGGCACACCCGCACC
CCGGCCGGCTTCTAA
Translation:
MLHQLTLAEIARALADKQFSAEELTRTLLGRIRQLDPQLNSFISITDDLAIAQAKAADERRANGENGALLGA
PIAHKDLFCTQGVRTSCGSKMLDNFVSPYDATVVEKLAAAGAVTLGKLNMDEFAMGSSNQSSHYGAVNNPWS
LDRVPGGSSGGSAAAVAARLLPAATGTDTGGSIRQPAALTNLTGIKPTYGRVSRWGMIAYASSLDQGGPLAR
TAEDCALMLGVMAGFDPKDSTSVEQPVDDYLAALQKPLSGLRIGLPREYFGAGLDSRIADAVLAVVEELKTL
GATVKDISLPNMQHAIPAYYVIAPAEASSNLSRFDGVRYGYRCDAPQNLEDLYKRSRAEGFGSEVKNRIMVG
TYALSAGYYDAYYLQAQKIRRLIKNDFVSAFAEVDVILGPTTPNPAWKIGEKNDDPVSQYLEDIYTITANLA
GLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQQVSDWHTRTPAGF*
AnnotationID:881GeneID:872
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 14:02:19
Gene Name:gatA
Confidence Code:2
GeneProduct:Glu-tRNA(Gln) amidotransferase subunit A
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0154,pfam01425,KOG1211
Secondary Functional Category(ies):
EC Number:3.5.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57923.1| (AE016872) glutamyl-tRNA(Gln) amidotransferase, A subunit
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 483

 Score =  813 bits (2100), Expect = 0.0
 Identities = 399/483 (82%), Positives = 439/483 (90%)

>gb|AAN66556.1|AE016284_4 (AE016777) glutamyl-tRNA(Gln) amidotransferase, A subunit
           [Pseudomonas putida KT2440]
          Length = 483

 Score =  810 bits (2091), Expect = 0.0
 Identities = 398/483 (82%), Positives = 438/483 (90%)

>gb|AAF41730.1| (AE002484) Glu-tRNA(Gln) amidotransferase, subunit A [Neisseria
           meningitidis MC58]
          Length = 481

 Score =  570 bits (1468), Expect = e-162
 Identities = 286/479 (59%), Positives = 356/479 (74%), Gaps = 2/479 (0%)







 
Structural Features:
COG0154, GatA, Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit 
and related amidases 
[Translation, ribosomal structure and biogenesis]
CD-Length = 475 residues,  99.6% aligned
Score =  517 bits (1334), Expect = 2e-147
Identities = 270/492 (54%), Positives = 329/492 (66%), Gaps = 29/492 (5%)

pfam01425, Amidase
CD-Length = 433 residues, 100.0% aligned
Score =  464 bits (1195), Expect = 2e-131
Identities = 241/449 (53%), Positives = 305/449 (67%), Gaps = 23/449 (5%)

KOG1211, Amidases 
[Translation, ribosomal structure and biogenesis]
CD-Length = 506 residues,  91.3% aligned
Score =  402 bits (1034), Expect = 1e-112
Identities = 204/463 (44%), Positives = 279/463 (60%), Gaps = 10/463 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1747




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18889




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31525




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:872_cdd.html


    		




 
URL:

















    ReferenceID:32886




  
Author/Investigator(s):
        Nobelmann B, Lengeler JW.





  
Title:
        Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism.




  
PubMed:
        8955298


MedLine:
        




    
Source:
J Bacteriol. 1996 Dec;178(23):6790-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8955298

















    ReferenceID:32887




  
Author/Investigator(s):
        Curnow AW, Hong K, Yuan R, Kim S, Martins O, Winkler W, Henkin TM, Soll D.





  
Title:
        Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.




  
PubMed:
        9342321


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11819-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9342321

















    ReferenceID:32888




  
Author/Investigator(s):
        Salazar JC, Zuniga R, Raczniak G, Becker H, Soll D, Orellana O.





  
Title:
        A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans.




  
PubMed:
        


MedLine:
        




    
Source:
FEBS Lett. 2001 Jul 6;500(3):129-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11445070

















    ReferenceID:32889




  
Author/Investigator(s):
        Raczniak G, Becker HD, Min B, Soll D.





  
Title:
        A single amidotransferase forms asparaginyl-tRNA and glutaminyl-tRNA in Chlamydia trachomatis.




  
PubMed:
        11585842


MedLine:
        




    
Source:
J Biol Chem. 2001 Dec 7;276(49):45862-7. Epub 2001 Oct 03.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11585842









           
Homologs By Global Alignment 

           

       



     
Gene ID:872
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1563
                           PA4483_tr
                           translation of PA4483
                           484
                           99.58
                           99.58
                           0
                           2469.0
     

     
     
56733
                           gb|AAG07871.1|AE004862_5
                           (AE004862) Glu-tRNA(Gln) amidotransferase subunit A [Pseudomonas aeruginosa PAO1]
                           484
                           99.58
                           99.58
                           0
                           2469.0
     

     
     
56734
                           gb|AAO57923.1|
                           (AE016872) glutamyl-tRNA(Gln) amidotransferase, A subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           484
                           82.43
                           90.70
                           1
                           2100.0
     

     
     
56735
                           gb|AAN66556.1|AE016284_4
                           (AE016777) glutamyl-tRNA(Gln) amidotransferase, A subunit [Pseudomonas putida KT2440]
                           484
                           82.23
                           90.49
                           1
                           2091.0
     

     
     
56736
                           gb|AAO90971.1|
                           (AE016964) glutamyl-tRNA(Gln) amidotransferase, A subunit [Coxiella burnetii RSA 493]
                           484
                           65.08
                           80.57
                           1
                           1687.0
     

     






      

          
GC ORFID: 43874How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4483Glimmer Score: 

          
Start: 4441244Stop: 4442698

          
Length: 1455


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4483 translation of PA4483Homolog Bit Score 955.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5564 (ORF: BLASTX 806.0), t_PA4483 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGCATCAATTGACCCTCGCCGAGATCGCCCGCGCCCTCGCCGACAAGCAATTTTCCGCCGAAGAGCTG 
ACCCGCACCCTGCTCGGTCGCATCCGGCAGCTGGATCCCCAGCTCAACAGCTTCATCAGCATCACCGACGAC 
CTGGCCATCGCCCAGGCCAAGGCAGCCGACGAACGGCGCGCCAACGGCGAGAACGGCGCCCTGCTCGGCGCG 
CCGATCGCCCACAAGGACCTGTTCTGCACCCAGGGCGTACGCACCAGCTGCGGTTCGAAGATGCTCGACAAC 
TTCGTCTCGCCCTACGACGCCACCGTGGTCGAGAAGCTCGCCGCCGCCGGCGCCGTTACCCTCGGCAAGCTG 
AACATGGACGAATTCGCCATGGGCTCGTCGAACCAGTCCAGCCACTACGGCGCGGTGAACAACCCCTGGAGC 
CTCGACCGCGTGCCTGGCGGCTCCTCCGGCGGCTCCGCCGCGGCAGTCGCCGCGCGCCTGCTGCCGGCCGCC 
ACCGGCACCGATACCGGCGGCTCGATCCGCCAGCCGGCGGCGCTGACCAACCTCACCGGGATCAAGCCGACC 
TACGGCCGGGTTTCCCGCTGGGGCATGATCGCCTACGCCTCCAGCCTCGACCAGGGCGGCCCGCTGGCGCGC 
ACCGCCGAGGACTGCGCGCTGATGCTGGGGGTGATGGCCGGATTCGATCCGAAGGACTCGACCAGCGTCGAA 
CAGCCGGTGGACGACTACCTGGCCGCCCTGCAGAAGCCGCTGAGCGGCCTGCGCATCGGCCTGCCGCGGGAA 
TACTTCGGCGCCGGCCTCGACAGCCGCATCGCCGACGCGGTGCTGGCCGTGGTCGAGGAGCTGAAGACGCTC 
GGCGCCACGGTGAAGGACATTTCCCTGCCGAACATGCAGCACGCCATACCGGCCTACTACGTGATCGCGCCG 
GCCGAGGCGTCCTCCAACCTGTCGCGCTTCGACGGCGTGCGCTATGGCTATCGTTGCGACGCCCCGCAGAAC 
CTGGAAGACCTGTACAAGCGCTCGCGCGCGGAAGGCTTCGGCAGCGAAGTGAAGAACCGCATCATGGTCGGC 
ACCTACGCACTGTCGGCCGGCTACTACGATGCCTATTACCTGCAGGCTCAGAAGATTCGCCGGCTGATCAAG 
AACGACTTCGTCAGCGCCTTTGCCGAAGTGGACGTGATCCTCGGCCCGACCACGCCGAACCCGGCCTGGAAG 
ATCGGCGAGAAGAACGACGACCCGGTTTCCCAGTACCTGGAAGACATCTACACCATCACCGCCAACCTCGCC 
GGCCTGCCGGGGCTGTCCATGCCCGCCGGCTTCGTCGACGGCCTGCCGGTGGGTGTCCAGTTGCTCGCGCCC 
TACTTCCAGGAAGGTCGCCTGCTCAACGTCGCGCACCAGTACCAGCAGGTCAGCGACTGGCACACCCGCACC 
CCGGCCGGCTTCTAA