ViewGene
Locus Name:
GeneID:
Location:

GeneID:869
TrivialName: PA14_67600
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67600
PAO1 Ortholog Locus:PA5119
Sequence Length:1410
Protein Length:469
Start: 6038737
Stop: 6040146
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08504.1|AE004925_2 (AE004925) glutamine synthetase [Pseudomonas aeruginosa PAO1]
Identities = 469/469 (100%), Positives = 469/469 (100%)
Sequence:
ATGTCGTACAAGTCGCACCAACTGATCAAAGACCATGACGTGAAGTGGGTAGACCTGCGCTTCACCGATACC
AAGGGCAAGCAGCAACACGTCACCATGCCGGCTCGCGACGCGCTGGACGATGAGTTCTTCGAAGCCGGCAAG
ATGTTCGACGGCTCCTCCATCGCCGGCTGGAAAGGCATCGAAGCCTCCGACATGATCCTGATGCCGGACGAC
AGCACCGCCGTCCTCGATCCGTTCACCGAAGAGCCGACCCTGATCCTGGTCTGCGACATCATCGAGCCGAGC
ACCATGCAAGGCTACGAGCGCGACCCGCGCAACATCGCCAAGCGCGCCGAGGAGTACCTGAAGTCCACCGGC
ATCGGCGACACCGTGTTCGTCGGCCCGGAGCCGGAGTTCTTCATCTTCGACGAAGTGAAGTTCAAGTCCGAC
ATCTCCGGCTCGATGTTCAAGATCTTCTCCGAGCAGGCTTCCTGGAACACCGACGCCGACATCGAGTCCGGC
AACAAGGGCCATCGCCCGGGCGTGAAGGGCGGCTACTTCCCGGTACCGCCGGTCGACCACGACCATGAAATC
CGCACCGCCATGTGCAACGCCCTGGAAGAAATGGGCCTGGTGGTCGAAGTCCACCACCACGAAGTGGCAACC
GCCGGCCAGAACGAGATCGGCGTGAAGTTCAACACCCTGGTCGCCAAGGCCGACGAAGTGCAGACCCTGAAG
TACTGCGTGCACAACGTCGCCGACGCCTACGGCAAGACCGTGACCTTCATGCCGAAGCCGCTGTACGGCGAC
AACGGTTCGGGCATGCACGTGCACATGTCGATCTCCAAGGACGGCAAGAACACCTTCGCCGGCGAAGGCTAT
GCCGGCCTGTCCGAGACCGCCCTGTACTTCATCGGCGGCATCATCAAGCACGGCAAGGCCCTGAACGGCTTC
ACCAACCCCTCGACCAACTCCTACAAGCGCCTGGTCCCGGGCTTCGAAGCTCCGGTGATGCTGGCCTACTCG
GCGCGCAACCGTTCCGCCTCGATCCGTATCCCCTACGTATCCAGCCCGAAGGCCCGCCGTATCGAAGCGCGC
TTCCCGGACCCGGCAGCCAACCCCTACCTGGCCTTCGCCGCGCTGCTGATGGCCGGCCTGGACGGCATCCAG
AACAAGATCCACCCCGGCGATGCCGCCGACAAGAACCTGTACGACCTGCCGCCGGAAGAGGCGAAGGAAATC
CCGCAGGTTTGCGGCAGCCTGAAAGAGGCGCTGGAAGAACTCGACAAGGGCCGCGCGTTCCTGACCAAGGGC
GGCGTGTTCACCGACGAGTTCATCGATGCCTACATCGAGCTGAAGAGCGAAGAAGAGATCAAGGTGCGCACC
TTCGTGCACCCGCTGGAATACGACCTGTACTACAGCGTCTGA
Translation:
MSYKSHQLIKDHDVKWVDLRFTDTKGKQQHVTMPARDALDDEFFEAGKMFDGSSIAGWKGIEASDMILMPDD
STAVLDPFTEEPTLILVCDIIEPSTMQGYERDPRNIAKRAEEYLKSTGIGDTVFVGPEPEFFIFDEVKFKSD
ISGSMFKIFSEQASWNTDADIESGNKGHRPGVKGGYFPVPPVDHDHEIRTAMCNALEEMGLVVEVHHHEVAT
AGQNEIGVKFNTLVAKADEVQTLKYCVHNVADAYGKTVTFMPKPLYGDNGSGMHVHMSISKDGKNTFAGEGY
AGLSETALYFIGGIIKHGKALNGFTNPSTNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVSSPKARRIEAR
FPDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEELDKGRAFLTKG
GVFTDEFIDAYIELKSEEEIKVRTFVHPLEYDLYYSV*
AnnotationID:878GeneID:869
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:19
Gene Name:glnA
Confidence Code:2
GeneProduct:glutamine synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00120,pfam03951
Secondary Functional Category(ies):
EC Number:6.3.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA62673.1| (M57275) glutamine synthetase [Azotobacter vinelandii]
          Length = 467

 Score =  905 bits (2339), Expect = 0.0
 Identities = 440/466 (94%), Positives = 456/466 (97%), Gaps = 1/466 (0%)

>gb|AAO53903.1| (AE016857) glutamine synthetase, type I [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 468

 Score =  881 bits (2276), Expect = 0.0
 Identities = 424/465 (91%), Positives = 445/465 (95%)

>gb|AAO09392.1|AE016799_290 (AE016799) Glutamine synthetase [Vibrio vulnificus CMCP6]
          Length = 469

 Score =  707 bits (1825), Expect = 0.0
 Identities = 338/462 (73%), Positives = 397/462 (85%), Gaps = 1/462 (0%)






 
Structural Features:
pfam00120, Gln-synt_C, Glutamine synthetase, catalytic domain.
CD-Length = 260 residues, 100.0% aligned
Score =  342 bits (880), Expect = 3e-95
Identities = 142/284 (50%), Positives = 168/284 (59%), Gaps = 26/284 (9%)

pfam03951, Gln-synt_N, Glutamine synthetase, beta-Grasp domain.
CD-Length = 84 residues,  98.8% aligned
Score =  117 bits (295), Expect = 2e-27
Identities = 46/83 (55%), Positives = 59/83 (71%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1741




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18883




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34933




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:869_cdd.html


    		




 
URL:

















    ReferenceID:35797




  
Author/Investigator(s):
        Miranda-Rios J, Sanchez-Pescador R, Urdea M, Covarrubias AA.





  
Title:
        The complete nucleotide sequence of the glnALG operon of Escherichia coli K12.




  
PubMed:
        2882477


MedLine:
        




    
Source:
Nucleic Acids Res. 1987 Mar 25;15(6):2757-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2882477

















    ReferenceID:35798




  
Author/Investigator(s):
        Zinchenko V, Churin Y, Shestopalov V, Shestakov S.





  
Title:
        Nucleotide sequence and characterization of the Rhodobacter sphaeroides glnB and glnA genes.




  
PubMed:
        7921264


MedLine:
        




    
Source:
Microbiology. 1994 Aug;140 ( Pt 8):2143-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7921264

















    ReferenceID:35799




  
Author/Investigator(s):
        Goodman HJ, Woods DR.





  
Title:
        Cloning and nucleotide sequence of the Butyrivibrio fibrisolvens gene encoding a type III glutamine synthetase.




  
PubMed:
        8103789


MedLine:
        




    
Source:
J Gen Microbiol. 1993 Jul;139 ( Pt 7):1487-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8103789

















    ReferenceID:35800




  
Author/Investigator(s):
        Sanangelantoni AM, Barbarini D, Di Pasquale G, Cammarano P, Tiboni O.





  
Title:
        Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: phylogenetic implications.




  
PubMed:
        1973523


MedLine:
        




    
Source:
Mol Gen Genet. 1990 Apr;221(2):187-94.






    
Reference Type: 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1973523

















    ReferenceID:35801




  
Author/Investigator(s):
        Colombo G, Villafranca JJ.





  
Title:
        Amino acid sequence of Escherichia coli glutamine synthetase deduced from the DNA nucleotide sequence.




  
PubMed:
        2874141


MedLine:
        




    
Source:
J Biol Chem. 1986 Aug 15;261(23):10587-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2874141









           
Homologs By Global Alignment 

           

       



     
Gene ID:869
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1560
                           PA5119_tr
                           translation of PA5119
                           469
                           99.99
                           99.99
                           0
                           2470.0
     

     
     
56046
                           gb|AAG08504.1|AE004925_2
                           (AE004925) glutamine synthetase [Pseudomonas aeruginosa PAO1]
                           469
                           99.99
                           99.99
                           0
                           2470.0
     

     
     
56047
                           gb|AAA62673.1|
                           (M57275) glutamine synthetase [Azotobacter vinelandii]
                           469
                           94.24
                           97.65
                           2
                           2340.0
     

     
     
56049
                           dbj|BAA90471.1|
                           (AB037422) glutamine synthetase [Pseudomonas syringae pv. tabaci]
                           469
                           91.04
                           95.09
                           1
                           2270.0
     

     
     
56048
                           gb|AAO53903.1|
                           (AE016857) glutamine synthetase, type I [Pseudomonas syringae pv. tomato str. DC3000]
                           469
                           90.83
                           95.30
                           1
                           2274.0
     

     






      

          
GC ORFID: 43871How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5119Glimmer Score: 

          
Start: 5302422Stop: 5303831

          
Length: 1410


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5119 translation of PA5119Homolog Bit Score 956.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6889 (ORF: BLASTX 901.0), t_PA5119 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGTACAAGTCGCACCAACTGATCAAAGACCATGACGTGAAGTGGGTAGACCTGCGCTTCACCGATACC 
AAGGGCAAGCAGCAACACGTCACCATGCCGGCTCGCGACGCGCTGGACGATGAGTTCTTCGAAGCCGGCAAG 
ATGTTCGACGGCTCCTCCATCGCCGGCTGGAAAGGCATCGAAGCCTCCGACATGATCCTGATGCCGGACGAC 
AGCACCGCCGTCCTCGATCCGTTCACCGAAGAGCCGACCCTGATCCTGGTCTGCGACATCATCGAGCCGAGC 
ACCATGCAAGGCTACGAGCGCGACCCGCGCAACATCGCCAAGCGCGCCGAGGAGTACCTGAAGTCCACCGGC 
ATCGGCGACACCGTGTTCGTCGGCCCGGAGCCGGAGTTCTTCATCTTCGACGAAGTGAAGTTCAAGTCCGAC 
ATCTCCGGCTCGATGTTCAAGATCTTCTCCGAGCAGGCTTCCTGGAACACCGACGCCGACATCGAGTCCGGC 
AACAAGGGCCATCGCCCGGGCGTGAAGGGCGGCTACTTCCCGGTACCGCCGGTCGACCACGACCATGAAATC 
CGCACCGCCATGTGCAACGCCCTGGAAGAAATGGGCCTGGTGGTCGAAGTCCACCACCACGAAGTGGCAACC 
GCCGGCCAGAACGAGATCGGCGTGAAGTTCAACACCCTGGTCGCCAAGGCCGACGAAGTGCAGACCCTGAAG 
TACTGCGTGCACAACGTCGCCGACGCCTACGGCAAGACCGTGACCTTCATGCCGAAGCCGCTGTACGGCGAC 
AACGGTTCGGGCATGCACGTGCACATGTCGATCTCCAAGGACGGCAAGAACACCTTCGCCGGCGAAGGCTAT 
GCCGGCCTGTCCGAGACCGCCCTGTACTTCATCGGCGGCATCATCAAGCACGGCAAGGCCCTGAACGGCTTC 
ACCAACCCCTCGACCAACTCCTACAAGCGCCTGGTCCCGGGCTTCGAAGCTCCGGTGATGCTGGCCTACTCG 
GCGCGCAACCGTTCCGCCTCGATCCGTATCCCCTACGTATCCAGCCCGAAGGCCCGCCGTATCGAAGCGCGC 
TTCCCGGACCCGGCAGCCAACCCCTACCTGGCCTTCGCCGCGCTGCTGATGGCCGGCCTGGACGGCATCCAG 
AACAAGATCCACCCCGGCGATGCCGCCGACAAGAACCTGTACGACCTGCCGCCGGAAGAGGCGAAGGAAATC 
CCGCAGGTTTGCGGCAGCCTGAAAGAGGCGCTGGAAGAACTCGACAAGGGCCGCGCGTTCCTGACCAAGGGC 
GGCGTGTTCACCGACGAGTTCATCGATGCCTACATCGAGCTGAAGAGCGAAGAAGAGATCAAGGTGCGCACC 
TTCGTGCACCCGCTGGAATACGACCTGTACTACAGCGTCTGA