ViewGene
Locus Name:
GeneID:
Location:

GeneID:868
TrivialName: PA14_67580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67580
PAO1 Ortholog Locus:PA5118
Sequence Length:1455
Protein Length:484
Start: 6038400
Stop: 6036946
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08503.1|AE004925_1 (AE004925) thiazole biosynthesis protein ThiI [Pseudomonas aeruginosa PAO1]
Identities = 482/484 (99%), Positives = 483/484 (99%)
Sequence:
ATGAAACTCATCGTCAAGACCTTCCAGGAAATCACCATCAAGAGCCGGCCGGTGCGCAAGCGCTTCATCCGG
CAGTTGGCGAAGAACATCCGCGCGGTGCTGCGCGACCTGGACCCGGAGCTGAAGGTCGAGGGCGAGTGGGAC
AACCTTGAGGTAGAGACCGCCGTGGTCGACGCCAAGGTCAGGCGCGAAATGATCGAGCGCCTGACCTGCACC
CCGGGCATCGGTCATTTCCTCGAGGTCCACGAGTATCCGCTGGGCGATTTCGACGACATCCTCGCCAAGTGC
AAGGCGCATTTCGGCGACCAGTTGGCCGGCAAGACCTTCGCCGTGCGCTGCAAGCGCGCCGGCAAGCACGCG
TTCACCTCGATGGAGGTGGAGCGTTACGTCGGCAGCGGCCTGCGCCGCGAATGCGGGGCTGCCGGGATCGAC
CTGAAGCAGCCGGAAGTCGAGGTGCGGATGGAAATCCGCCTCGACCGCCTGTTCGTCATCCATCGCCAGCAT
CCGGGCCTGGGCGGCTATCCGCTGGGCGCGCTGGAACAGGTGCTGGTGCTGATGTCCGGCGGCTTCGACTCG
ACCGTGGCGGCCTACCAGATGATGCGCCGCGGGATGATCAGCCACTTCGTGTTCTTCAACCTCGGCGGGCGC
GCCCACGAACTGGGGGTGATGGAAGTCGCCCACTACCTGTGGGAGAAGTACGGCCGCTCGCAGCGCGTGCTG
TTCATCAGCGTGCCGTTCGAGGAAGTGGTCGGCGAGATCCTCACCAAGGTCGACGACAGCTATATGGGCGTG
ACCCTCAAGCGCATGATGCTGCGCGCCGCCAGCCGCGTGGCCGAGCGCCTGGAGCTGGACGCGCTGGTGACC
GGCGAGGCGATCTCCCAGGTGTCCAGCCAGACCCTGCCGAACCTCTCGGTGATCGACCGGGTTACCGACACC
CTGGTGCTGCGCCCGCTGATCGTCAGCCACAAGCAGGACATCATCGACACCGCCCGGCAGATCGGCACCGCC
GAGTTCGCCCGGCACATGCCGGAATACTGCGGGGTGATCTCGGTGAACCCGACCACCCAGGCCAAGCCCTAC
CGCGTCGAGCACGAAGAGTCGAAATTCGACATGGCGGTGCTCGAGCGCGCCCTGGAGCGCGCCACCCAGCGC
ACCGTCGACCGGGTGATCGACGAACTCGGCCAGGACCTGCAGGTGGAAGAGGTCGGCGAGGTGCTGCCCGGT
CAGATTGTCATCGATATCCGCCATCCTGATGCCCAGGAAGACGAACCCCTGGCCCTGGAAGGCGTCGAAGTC
CAGGCGCTGCCGTTCTACGCGATCAACAGCCGCTTCAAGGAACTGGACGCCAACCGCCAGTACCTCCTGTAT
TGCGACAAAGGGGTGATGAGCCGCCTGCATGCCCATCATCTGCTCAACGAGGGGCACACCAATGTGCGTGTT
TATCGTCCGGCTTAA
Translation:
MKLIVKTFQEITIKSRPVRKRFIRQLAKNIRAVLRDLDPELKVEGEWDNLEVETAVVDAKVRREMIERLTCT
PGIGHFLEVHEYPLGDFDDILAKCKAHFGDQLAGKTFAVRCKRAGKHAFTSMEVERYVGSGLRRECGAAGID
LKQPEVEVRMEIRLDRLFVIHRQHPGLGGYPLGALEQVLVLMSGGFDSTVAAYQMMRRGMISHFVFFNLGGR
AHELGVMEVAHYLWEKYGRSQRVLFISVPFEEVVGEILTKVDDSYMGVTLKRMMLRAASRVAERLELDALVT
GEAISQVSSQTLPNLSVIDRVTDTLVLRPLIVSHKQDIIDTARQIGTAEFARHMPEYCGVISVNPTTQAKPY
RVEHEESKFDMAVLERALERATQRTVDRVIDELGQDLQVEEVGEVLPGQIVIDIRHPDAQEDEPLALEGVEV
QALPFYAINSRFKELDANRQYLLYCDKGVMSRLHAHHLLNEGHTNVRVYRPA*
AnnotationID:877GeneID:868
AnnotatorUID: diggins
Modification Date/Time: 2005-03-15 08:08:49
Gene Name:thiI
Confidence Code:2
GeneProduct:thiamin biosynthesis protein ThiI
Cell Localization:()
Synonyms:yajK
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0301,pfam02568
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70610.1|AE016704_1 (AE016793) thiamine biosynthesis protein ThiI [Pseudomonas putida
           KT2440]
          Length = 484

 Score =  791 bits (2044), Expect = 0.0
 Identities = 390/483 (80%), Positives = 432/483 (89%)

>gb|AAO53904.1| (AE016857) thiamin biosynthesis protein ThiI [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 484

 Score =  728 bits (1880), Expect = 0.0
 Identities = 352/484 (72%), Positives = 418/484 (86%)

>gb|AAG54773.1|AE005221_10 (AE005221) putative oxidoreductase [Escherichia coli O157:H7
           EDL933]
          Length = 482

 Score =  503 bits (1295), Expect = e-141
 Identities = 256/484 (52%), Positives = 339/484 (70%), Gaps = 3/484 (0%)






 
Structural Features:
COG0301, ThiI, Thiamine biosynthesis ATP pyrophosphatase [Coenzyme metabolism]
CD-Length = 383 residues,  99.7% aligned
Score =  391 bits (1006), Expect = 1e-109
Identities = 164/388 (42%), Positives = 219/388 (56%), Gaps = 8/388 (2%)

pfam02568, ThiI, Thiamine biosynthesis protein (ThiI)
CD-Length = 197 residues,  99.5% aligned
Score =  193 bits (492), Expect = 3e-50
Identities = 81/196 (41%), Positives = 111/196 (56%), Gaps = 2/196 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1739




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18881




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34954




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:868_cdd.html


    		




 
URL:

















    ReferenceID:35802




  
Author/Investigator(s):
        Webb E, Claas K, Downs DM.





  
Title:
        Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.




  
PubMed:
        9209060


MedLine:
        




    
Source:
J Bacteriol. 1997 Jul;179(13):4399-402.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9209060









           
Homologs By Global Alignment 

           

       



     
Gene ID:868
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1559
                           PA5118_tr
                           translation of PA5118
                           484
                           99.58
                           99.79
                           0
                           2472.0
     

     
     
56021
                           gb|AAG08503.1|AE004925_1
                           (AE004925) thiazole biosynthesis protein ThiI [Pseudomonas aeruginosa PAO1]
                           484
                           99.58
                           99.79
                           0
                           2472.0
     

     
     
56022
                           gb|AAN70610.1|AE016704_1
                           (AE016793) thiamine biosynthesis protein ThiI [Pseudomonas putida KT2440]
                           484
                           80.57
                           89.25
                           0
                           2044.0
     

     
     
56023
                           gb|AAO53904.1|
                           (AE016857) thiamin biosynthesis protein ThiI [Pseudomonas syringae pv. tomato str. DC3000]
                           484
                           72.72
                           86.36
                           0
                           1880.0
     

     
     
56024
                           gb|AAG54773.1|AE005221_10
                           (AE005221) putative oxidoreductase [Escherichia coli O157:H7 EDL933]
                           485
                           52.78
                           69.89
                           4
                           1299.0
     

     






      

          
GC ORFID: 43870How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5118Glimmer Score: 

          
Start: 5302085Stop: 5300631

          
Length: 1455


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5118 translation of PA5118Homolog Bit Score 956.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6548 (ORF: BLASTX 729.0), t_PA5118 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACTCATCGTCAAGACCTTCCAGGAAATCACCATCAAGAGCCGGCCGGTGCGCAAGCGCTTCATCCGG 
CAGTTGGCGAAGAACATCCGCGCGGTGCTGCGCGACCTGGACCCGGAGCTGAAGGTCGAGGGCGAGTGGGAC 
AACCTTGAGGTAGAGACCGCCGTGGTCGACGCCAAGGTCAGGCGCGAAATGATCGAGCGCCTGACCTGCACC 
CCGGGCATCGGTCATTTCCTCGAGGTCCACGAGTATCCGCTGGGCGATTTCGACGACATCCTCGCCAAGTGC 
AAGGCGCATTTCGGCGACCAGTTGGCCGGCAAGACCTTCGCCGTGCGCTGCAAGCGCGCCGGCAAGCACGCG 
TTCACCTCGATGGAGGTGGAGCGTTACGTCGGCAGCGGCCTGCGCCGCGAATGCGGGGCTGCCGGGATCGAC 
CTGAAGCAGCCGGAAGTCGAGGTGCGGATGGAAATCCGCCTCGACCGCCTGTTCGTCATCCATCGCCAGCAT 
CCGGGCCTGGGCGGCTATCCGCTGGGCGCGCTGGAACAGGTGCTGGTGCTGATGTCCGGCGGCTTCGACTCG 
ACCGTGGCGGCCTACCAGATGATGCGCCGCGGGATGATCAGCCACTTCGTGTTCTTCAACCTCGGCGGGCGC 
GCCCACGAACTGGGGGTGATGGAAGTCGCCCACTACCTGTGGGAGAAGTACGGCCGCTCGCAGCGCGTGCTG 
TTCATCAGCGTGCCGTTCGAGGAAGTGGTCGGCGAGATCCTCACCAAGGTCGACGACAGCTATATGGGCGTG 
ACCCTCAAGCGCATGATGCTGCGCGCCGCCAGCCGCGTGGCCGAGCGCCTGGAGCTGGACGCGCTGGTGACC 
GGCGAGGCGATCTCCCAGGTGTCCAGCCAGACCCTGCCGAACCTCTCGGTGATCGACCGGGTTACCGACACC 
CTGGTGCTGCGCCCGCTGATCGTCAGCCACAAGCAGGACATCATCGACACCGCCCGGCAGATCGGCACCGCC 
GAGTTCGCCCGGCACATGCCGGAATACTGCGGGGTGATCTCGGTGAACCCGACCACCCAGGCCAAGCCCTAC 
CGCGTCGAGCACGAAGAGTCGAAATTCGACATGGCGGTGCTCGAGCGCGCCCTGGAGCGCGCCACCCAGCGC 
ACCGTCGACCGGGTGATCGACGAACTCGGCCAGGACCTGCAGGTGGAAGAGGTCGGCGAGGTGCTGCCCGGT 
CAGATTGTCATCGATATCCGCCATCCTGATGCCCAGGAAGACGAACCCCTGGCCCTGGAAGGCGTCGAAGTC 
CAGGCGCTGCCGTTCTACGCGATCAACAGCCGCTTCAAGGAACTGGACGCCAACCGCCAGTACCTCCTGTAT 
TGCGACAAAGGGGTGATGAGCCGCCTGCATGCCCATCATCTGCTCAACGAGGGGCACACCAATGTGCGTGTT 
TATCGTCCGGCTTAA