ViewGene
Locus Name:
GeneID:
Location:

GeneID:867
TrivialName: PA14_23030
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23030
PAO1 Ortholog Locus:PA3186
Sequence Length:1365
Protein Length:454
Start: 1996676
Stop: 1998040
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06574.1|AE004742_10 (AE004742) Glucose/carbohydrate outer membrane porin OprB precursor [Pseudomonas aeruginosa PAO1]
Identities = 454/454 (100%), Positives = 454/454 (100%)
Sequence:
ATGTACAAGAACAAGAAAACCAGACCGGCCGCCAGGACCGTTGGCTGCCTGTTTGCCCTTGGAGCGCTGGGC
TTGGGGAGCGCGGCGCACGCCGCCGAGGCGTTCTCCCCGAACTCGAAATGGATGCTCGGCGATTGGGGCGGC
AAGCGCACCGAGCTGCTGGAGAAGGGCTACGACTTCAAGCTGGAGTACGTCGGCGAGGCGGCGGCCAACCTC
GATGGCGGCTATGACGACGACAAGACCGGACGCTACACCGACCAGTTCGCCCTGGGCGTGCACATGGACCTG
GAGAAGATCCTCGGCTGGAAGGCTACCGAGTTCCAGTTCACCGTCACCGAGCGCAACGGCAAGAACCTTTCC
AACGACCGCATCGGCGACCCGCGTGCCGGGCACATCAGCTCGGTGCAGGAGGTCTGGGGCCGCGGGCAGACC
TGGCGGCTGACCCAGCTGTGGCTCAAGCAGCAGTACTTCGACGGCGCGCTGGACGTGAAATTCGGCCGTTTC
GGCGAGGGCGAGGACTTCAACAGCTTCCCCTGCGATTTCCAGAACCTGGCCTTCTGCGGCTCGCAGGTGGGC
AACTGGGCGGGGAGCATCTGGTACAACTGGCCGGTCAGCCAGTGGGCGTTGCGGGTGAAGTACAACTTCGCG
CCGGACTGGTACGTGCAGGTCGGCGCCTACGAGCAGAACCCGTCGAACCTGGAGACCGGCAACGGCTTCAAG
ATGAGCGGCAGCGGGACCAAGGGCGCGCTGCTGCCGGTGGAGCTGATCTGGCAGCCGAAGGTCGGCGCCGAG
CAACTGCCGGGCGAGTACCGGCTGGGCTACTACTACAGCACGGCGAAGGCCGATGACGTCTACGACGACGTC
GACGGCCAGCCGCAGGGGCTGACCGGCAACGACTTCAAGTCGCGCGGCAGCAAGCATGGCTGGTGGGTGGTG
GCGCAGCAGCAGGTCACTTCGCACAACGGCGATGCTTCGCGCGGGTTGAGCCTGTTCGCCAACCTGACGGTC
CACGACAAGGCGACCAACGTGGTGGACAACTACCAGCAGCTCGGGGTGGTCTACAAGGGTCCGTTCGATGCG
CGGCCGAAGGACGACATCGGCCTGGGCATCGCGCGCATCCACGTCAACGACGATGTGAAGAAGCGCCAGCGC
CTGGTGAACCAGGTGAACGGCATCGACGATTACGACAACCCGCTGTACCAGCCGCTGCAGGACACCGAGTAC
AACGCCGAGCTGTACTACGGGGTGCATGTGACCGACTGGCTGACGGTGCGGCCGAACCTGCAATACATCAAG
CAGCCGGGCGGGGTCGACGAGGTCGACAACGCGCTGGTGGCGGGGATCAAGATCCAGACGGTGTTCTGA
Translation:
MYKNKKTRPAARTVGCLFALGALGLGSAAHAAEAFSPNSKWMLGDWGGKRTELLEKGYDFKLEYVGEAAANL
DGGYDDDKTGRYTDQFALGVHMDLEKILGWKATEFQFTVTERNGKNLSNDRIGDPRAGHISSVQEVWGRGQT
WRLTQLWLKQQYFDGALDVKFGRFGEGEDFNSFPCDFQNLAFCGSQVGNWAGSIWYNWPVSQWALRVKYNFA
PDWYVQVGAYEQNPSNLETGNGFKMSGSGTKGALLPVELIWQPKVGAEQLPGEYRLGYYYSTAKADDVYDDV
DGQPQGLTGNDFKSRGSKHGWWVVAQQQVTSHNGDASRGLSLFANLTVHDKATNVVDNYQQLGVVYKGPFDA
RPKDDIGLGIARIHVNDDVKKRQRLVNQVNGIDDYDNPLYQPLQDTEYNAELYYGVHVTDWLTVRPNLQYIK
QPGGVDEVDNALVAGIKIQTVF*
AnnotationID:876GeneID:867
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 06:06:29
Gene Name:oprB
Confidence Code:1
GeneProduct:glucose/carbohydrate outer membrane porin OprB precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:carbohydrate-selective porin
Functional Category Confidence Code:5
COGs:pfam04966,COG3659
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG05679.1|AE004655_1 (AE004655) probable glucose-sensitive porin [Pseudomonas aeruginosa
           PAO1]
          Length = 452

 Score =  902 bits (2330), Expect = 0.0
 Identities = 436/453 (96%), Positives = 438/453 (96%), Gaps = 3/453 (0%)

>gb|AAN66644.1|AE016292_4 (AE016777) porin B [Pseudomonas putida KT2440]
          Length = 447

 Score =  748 bits (1930), Expect = 0.0
 Identities = 349/447 (78%), Positives = 385/447 (86%), Gaps = 3/447 (0%)








 
Structural Features:
pfam04966, OprB, Carbohydrate-selective porin, OprB family.
CD-Length = 355 residues, 100.0% aligned
Score =  397 bits (1020), Expect = 2e-111
Identities = 173/374 (46%), Positives = 225/374 (60%), Gaps = 28/374 (7%)

COG3659, OprB, Carbohydrate-selective porin 
[Cell envelope biogenesis, outer membrane]
CD-Length = 439 residues,  99.8% aligned
Score =  551 bits (1421), Expect = 6e-158
Identities = 313/452 (69%), Positives = 344/452 (76%), Gaps = 14/452 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1737




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18879




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32357




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:867_cdd.html


    		




 
URL:

















    ReferenceID:33968




  
Author/Investigator(s):
        Wylie JL, Worobec EA.





  
Title:
        Cloning and nucleotide sequence of the Pseudomonas aeruginosa glucose-selective OprB porin gene and distribution of OprB within the family Pseudomonadaceae.




  
PubMed:
        8125108


MedLine:
        




    
Source:
Eur J Biochem. 1994 Mar 1;220(2):505-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8125108

















    ReferenceID:33969




  
Author/Investigator(s):
        Wylie JL, Worobec EA.





  
Title:
        The OprB porin plays a central role in carbohydrate uptake in Pseudomonas aeruginosa.




  
PubMed:
        7768797


MedLine:
        




    
Source:
J Bacteriol. 1995 Jun;177(11):3021-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7768797

















    ReferenceID:33970




  
Author/Investigator(s):
        Wylie JL, Bernegger-Egli C, O'Neil JD, Worobec EA.





  
Title:
        Biophysical characterization of OprB, a glucose-inducible porin of Pseudomonas aeruginosa.




  
PubMed:
        8132494


MedLine:
        




    
Source:
J Bioenerg Biomembr. 1993 Oct;25(5):547-56.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8132494









           
Homologs By Global Alignment 

           

       



     
Gene ID:867
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1557
                           PA3186_tr
                           translation of PA3186
                           454
                           99.99
                           99.99
                           0
                           2470.0
     

     
     
56013
                           gb|AAG06574.1|AE004742_10
                           (AE004742) Glucose/carbohydrate outer membrane porin OprB precursor [Pseudomonas aeruginosa PAO1]
                           454
                           99.99
                           99.99
                           0
                           2470.0
     

     
     
56014
                           emb|CAA54387.1|
                           (X77131) porin [Pseudomonas aeruginosa]
                           454
                           99.99
                           99.99
                           0
                           2470.0
     

     
     
1558
                           PA2291_tr
                           translation of PA2291
                           455
                           95.82
                           96.26
                           4
                           2333.0
     

     
     
56015
                           gb|AAG05679.1|AE004655_1
                           (AE004655) probable glucose-sensitive porin [Pseudomonas aeruginosa PAO1]
                           455
                           95.82
                           96.26
                           4
                           2333.0
     

     






      

          
GC ORFID: 43869How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3186Glimmer Score: 

          
Start: 2957255Stop: 2955891

          
Length: 1365


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3186 translation of PA3186Homolog Bit Score 956.0

          
Other Homologs:  t_PA2291 (ORF: BLASTX 902.0), psyr_15may02_Scaffold3_revised_gene4422 (ORF: BLASTX 706.0), t_PA3186 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTACAAGAACAAGAAAACCAGACCGGCCGCCAGGACCGTTGGCTGCCTGTTTGCCCTTGGAGCGCTGGGC 
TTGGGGAGCGCGGCGCACGCCGCCGAGGCGTTCTCCCCGAACTCGAAATGGATGCTCGGCGATTGGGGCGGC 
AAGCGCACCGAGCTGCTGGAGAAGGGCTACGACTTCAAGCTGGAGTACGTCGGCGAGGCGGCGGCCAACCTC 
GATGGCGGCTATGACGACGACAAGACCGGACGCTACACCGACCAGTTCGCCCTGGGCGTGCACATGGACCTG 
GAGAAGATCCTCGGCTGGAAGGCTACCGAGTTCCAGTTCACCGTCACCGAGCGCAACGGCAAGAACCTTTCC 
AACGACCGCATCGGCGACCCGCGTGCCGGGCACATCAGCTCGGTGCAGGAGGTCTGGGGCCGCGGGCAGACC 
TGGCGGCTGACCCAGCTGTGGCTCAAGCAGCAGTACTTCGACGGCGCGCTGGACGTGAAATTCGGCCGTTTC 
GGCGAGGGCGAGGACTTCAACAGCTTCCCCTGCGATTTCCAGAACCTGGCCTTCTGCGGCTCGCAGGTGGGC 
AACTGGGCGGGGAGCATCTGGTACAACTGGCCGGTCAGCCAGTGGGCGTTGCGGGTGAAGTACAACTTCGCG 
CCGGACTGGTACGTGCAGGTCGGCGCCTACGAGCAGAACCCGTCGAACCTGGAGACCGGCAACGGCTTCAAG 
ATGAGCGGCAGCGGGACCAAGGGCGCGCTGCTGCCGGTGGAGCTGATCTGGCAGCCGAAGGTCGGCGCCGAG 
CAACTGCCGGGCGAGTACCGGCTGGGCTACTACTACAGCACGGCGAAGGCCGATGACGTCTACGACGACGTC 
GACGGCCAGCCGCAGGGGCTGACCGGCAACGACTTCAAGTCGCGCGGCAGCAAGCATGGCTGGTGGGTGGTG 
GCGCAGCAGCAGGTCACTTCGCACAACGGCGATGCTTCGCGCGGGTTGAGCCTGTTCGCCAACCTGACGGTC 
CACGACAAGGCGACCAACGTGGTGGACAACTACCAGCAGCTCGGGGTGGTCTACAAGGGTCCGTTCGATGCG 
CGGCCGAAGGACGACATCGGCCTGGGCATCGCGCGCATCCACGTCAACGACGATGTGAAGAAGCGCCAGCGC 
CTGGTGAACCAGGTGAACGGCATCGACGATTACGACAACCCGCTGTACCAGCCGCTGCAGGACACCGAGTAC 
AACGCCGAGCTGTACTACGGGGTGCATGTGACCGACTGGCTGACGGTGCGGCCGAACCTGCAATACATCAAG 
CAGCCGGGCGGGGTCGACGAGGTCGACAACGCGCTGGTGGCGGGGATCAAGATCCAGACGGTGTTCTGA