ViewGene
Locus Name:
GeneID:
Location:

GeneID:865
TrivialName: PA14_48060
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48060
PAO1 Ortholog Locus:PA1249
Sequence Length:1440
Protein Length:479
Start: 4278964
Stop: 4277525
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04638.1|AE004554_6 (AE004554) alkaline metalloproteinase precursor [Pseudomonas aeruginosa PAO1]
Identities = 477/479 (99%), Positives = 479/479 (100%)
Sequence:
ATGTCCAGCAATTCTCTTGCATTGAAAGGTCGTAGTGATGCGTATACCCAGGTAGACAACTTCCTGCATGCC
TATGCGCGGGGCGGGGACGAATTGGTCAATGGCCATCCGTCCTATACCGTAGACCAGGCGGCGGAGCAGATC
CTCCGCGAACAGGCGTCTTGGCAGAAAGCACCGGGCGACTCGGTGCTGACCCTGTCCTATTCGTTCCTGACC
AAGCCGAACGACTTCTTCAATACGCCGTGGAAGTATGTCAGCGATATCTACTCGCTGGGCAAGTTCAGCGCC
TTCTCCGCGCAGCAGCAGGCCCAGGCCAAGTTGTCGCTGCAATCCTGGGCGGACGTCACCAATATCCACTTC
GTCGACGCCGGCCAGGGCGATCAGGGCGACCTGACCTTCGGCAACTTCAGCAGTAGTGTCGGCGGTGCGGCG
TTCGCCTTCCTGCCGGATGTACCGGATGCGCTCAAGGGGCAATCCTGGTACCTGATCAACAGCAGCTACAGC
GCCAACGTCAATCCGGCCAACGGCAACTACGGACGCCAGACGCTGACCCACGAGATCGGCCATACCCTGGGC
CTGAGCCACCCCGGCGACTACAACGCCGGCGAGGGCGATCCCACCTACGCCGACGCTACCTACGCCGAGGAC
ACCCGCGCCTATTCGGTGATGAGCTACTGGGAAGAGCAGAACACCGGCCAGGACTTCAAGGGCGCCTATTCC
TCGGCACCGCTGCTGGACGACATCGCGGCGATCCAGAAGCTCTACGGGGCCAACCTGACCACTCGCACCGGC
GACACGGTGTACGGCTTCAACTCCAACACCGAGCGCGACTTCTACAGCGCCACCTCGTCCAGTTCCAAGCTG
GTGTTCTCGGTGTGGGACGCCGGCGGCAACGACACCCTGGACTTCTCCGGCTTCAGCCAGAACCAGAAGATC
AACCTCAACGAGAAGGCGCTGTCCGATGTCGGCGGGTTGAAGGGCAATGTGTCGATCGCTGCCGGGGTCACC
GTGGAAAACGCCATCGGCGGCTCGGGTAGCGACCTGTTGATCGGCAACGACGTGGCCAACGTGCTCAAGGGC
GGCGCCGGCAACGACATCCTCTACGGCGGCCTCGGCGCGGACCAGCTGTGGGGCGGCGCGGGAGCCGACACC
TTCGTCTACGGCGATATCGCCGAGTCCTCCGCGGCGGCGCCGGATACCCTGCGCGACTTCGTCAGCGGCCAG
GACAAGATCGACCTGTCCGGGCTGGACGCCTTCGTCAACGGCGGGCTGGTGCTGCAATACGTCGACGCCTTC
GCCGGCCAGGCCGGCCAGGCGATCCTGTCCTACGACGCGGCGAGCAAGGCCGGCAGCCTGGCGATCGACTTC
AGCGGGGATGCCCATGCCGATTTCGCGATCAATCTGATCGGCCAGGCGACCCAGGCCGACATCGTCGTCTGA
Translation:
MSSNSLALKGRSDAYTQVDNFLHAYARGGDELVNGHPSYTVDQAAEQILREQASWQKAPGDSVLTLSYSFLT
KPNDFFNTPWKYVSDIYSLGKFSAFSAQQQAQAKLSLQSWADVTNIHFVDAGQGDQGDLTFGNFSSSVGGAA
FAFLPDVPDALKGQSWYLINSSYSANVNPANGNYGRQTLTHEIGHTLGLSHPGDYNAGEGDPTYADATYAED
TRAYSVMSYWEEQNTGQDFKGAYSSAPLLDDIAAIQKLYGANLTTRTGDTVYGFNSNTERDFYSATSSSSKL
VFSVWDAGGNDTLDFSGFSQNQKINLNEKALSDVGGLKGNVSIAAGVTVENAIGGSGSDLLIGNDVANVLKG
GAGNDILYGGLGADQLWGGAGADTFVYGDIAESSAAAPDTLRDFVSGQDKIDLSGLDAFVNGGLVLQYVDAF
AGQAGQAILSYDAASKAGSLAIDFSGDAHADFAINLIGQATQADIVV*
AnnotationID:874GeneID:865
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:15
Gene Name:aprA
Confidence Code:1
GeneProduct:alkaline metalloproteinase precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:alkaline protease
Functional Category Confidence Code:5
COGs:cd00203,smart00235
Secondary Functional Category(ies):
EC Number:3.4.24.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56810.1| (AE016868) protease PrtA [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 475

 Score =  581 bits (1498), Expect = e-165
 Identities = 302/474 (63%), Positives = 357/474 (75%), Gaps = 21/474 (4%)

>gb|AAP44489.1| (AY298902) extracellular alkaline metalloprotease [Pseudomonas
           fluorescens]
          Length = 477

 Score =  560 bits (1443), Expect = e-159
 Identities = 281/472 (59%), Positives = 345/472 (73%), Gaps = 16/472 (3%)

>gb|AAO39138.1| (AY230750) secreted alkaline metalloproteinase [Photorhabdus
           luminescens]
          Length = 486

 Score =  454 bits (1169), Expect = e-127
 Identities = 250/470 (53%), Positives = 321/470 (68%), Gaps = 25/470 (5%)






 
Structural Features:
cd00203, ZnMc, Zinc-dependent metalloprotease; Neutral zinc metallopeptidases.
CD-Length = 165 residues,  97.6% aligned
Score =  114 bits (285), Expect = 3e-26
Identities = 45/169 (26%), Positives = 63/169 (37%), Gaps = 9/169 (5%)

smart00235, ZnMc, Zinc-dependent metalloprotease; Neutral zinc metallopeptidases.
CD-Length = 144 residues,  97.2% aligned
Score = 75.1 bits (184), Expect = 2e-14
Identities = 36/160 (22%), Positives = 56/160 (35%), Gaps = 20/160 (12%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1733




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18875




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37544




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:865_cdd.html


    		




 
URL:

















    ReferenceID:38038




  
Author/Investigator(s):
        Duong F, Lazdunski A, Cami B, Murgier M.





  
Title:
        Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.




  
PubMed:
        1427098


MedLine:
        




    
Source:
Gene. 1992 Nov 2;121(1):47-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1427098









           
Homologs By Global Alignment 

           

       



     
Gene ID:865
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1554
                           PA1249_tr
                           translation of PA1249
                           479
                           99.58
                           99.99
                           0
                           2472.0
     

     
     
55955
                           gb|AAG04638.1|AE004554_6
                           (AE004554) alkaline metalloproteinase precursor [Pseudomonas aeruginosa PAO1]
                           479
                           99.58
                           99.99
                           0
                           2472.0
     

     
     
55956
                           emb|CAA45858.1|
                           (X64558) alkaline protease [Pseudomonas aeruginosa]
                           479
                           99.58
                           99.99
                           0
                           2472.0
     

     
     
55957
                           gb|AAQ75527.1|
                           (AY367764) organic solvent-tolerant protease [Pseudomonas aeruginosa]
                           479
                           99.16
                           99.79
                           0
                           2465.0
     

     
     
55958
                           dbj|BAA13503.1|
                           (D87921) alkaline proteinase [Pseudomonas aeruginosa]
                           479
                           94.78
                           96.03
                           3
                           2317.0
     

     






      

          
GC ORFID: 43867How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1249Glimmer Score: 

          
Start: 674945Stop: 676384

          
Length: 1440


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1249 translation of PA1249Homolog Bit Score 956.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2825 (ORF: BLASTX 591.0), t_PA1249 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCAGCAATTCTCTTGCATTGAAAGGTCGTAGTGATGCGTATACCCAGGTAGACAACTTCCTGCATGCC 
TATGCGCGGGGCGGGGACGAATTGGTCAATGGCCATCCGTCCTATACCGTAGACCAGGCGGCGGAGCAGATC 
CTCCGCGAACAGGCGTCTTGGCAGAAAGCACCGGGCGACTCGGTGCTGACCCTGTCCTATTCGTTCCTGACC 
AAGCCGAACGACTTCTTCAATACGCCGTGGAAGTATGTCAGCGATATCTACTCGCTGGGCAAGTTCAGCGCC 
TTCTCCGCGCAGCAGCAGGCCCAGGCCAAGTTGTCGCTGCAATCCTGGGCGGACGTCACCAATATCCACTTC 
GTCGACGCCGGCCAGGGCGATCAGGGCGACCTGACCTTCGGCAACTTCAGCAGTAGTGTCGGCGGTGCGGCG 
TTCGCCTTCCTGCCGGATGTACCGGATGCGCTCAAGGGGCAATCCTGGTACCTGATCAACAGCAGCTACAGC 
GCCAACGTCAATCCGGCCAACGGCAACTACGGACGCCAGACGCTGACCCACGAGATCGGCCATACCCTGGGC 
CTGAGCCACCCCGGCGACTACAACGCCGGCGAGGGCGATCCCACCTACGCCGACGCTACCTACGCCGAGGAC 
ACCCGCGCCTATTCGGTGATGAGCTACTGGGAAGAGCAGAACACCGGCCAGGACTTCAAGGGCGCCTATTCC 
TCGGCACCGCTGCTGGACGACATCGCGGCGATCCAGAAGCTCTACGGGGCCAACCTGACCACTCGCACCGGC 
GACACGGTGTACGGCTTCAACTCCAACACCGAGCGCGACTTCTACAGCGCCACCTCGTCCAGTTCCAAGCTG 
GTGTTCTCGGTGTGGGACGCCGGCGGCAACGACACCCTGGACTTCTCCGGCTTCAGCCAGAACCAGAAGATC 
AACCTCAACGAGAAGGCGCTGTCCGATGTCGGCGGGTTGAAGGGCAATGTGTCGATCGCTGCCGGGGTCACC 
GTGGAAAACGCCATCGGCGGCTCGGGTAGCGACCTGTTGATCGGCAACGACGTGGCCAACGTGCTCAAGGGC 
GGCGCCGGCAACGACATCCTCTACGGCGGCCTCGGCGCGGACCAGCTGTGGGGCGGCGCGGGAGCCGACACC 
TTCGTCTACGGCGATATCGCCGAGTCCTCCGCGGCGGCGCCGGATACCCTGCGCGACTTCGTCAGCGGCCAG 
GACAAGATCGACCTGTCCGGGCTGGACGCCTTCGTCAACGGCGGGCTGGTGCTGCAATACGTCGACGCCTTC 
GCCGGCCAGGCCGGCCAGGCGATCCTGTCCTACGACGCGGCGAGCAAGGCCGGCAGCCTGGCGATCGACTTC 
AGCGGGGATGCCCATGCCGATTTCGCGATCAATCTGATCGGCCAGGCGACCCAGGCCGACATCGTCGTCTGA