ViewGene
Locus Name:
GeneID:
Location:

GeneID:864
TrivialName: PA14_22690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22690
PAO1 Ortholog Locus:PA3210
Sequence Length:1455
Protein Length:484
Start: 1975013
Stop: 1973559
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06598.1|AE004744_13 (AE004744) potassium uptake protein TrkH [Pseudomonas aeruginosa PAO1]
Identities = 483/484 (99%), Positives = 483/484 (99%)
Sequence:
ATGGCCTTGCCGACCCTACGCATCCTCGGTTTCATCATCGGCATCTTCCTGATCACTCTGGCCGCCAGCATG
CTGGTCCCGATCCTGACCATGCTCGCCTTCGACCGTACCGAAGGCATCCAGGCGTTCATCTGGTCCAGCGTC
GCCACCTTCGTCACCGGCCTCGGCCTGGTCCTGCCGGGGCGTCCCGAGCATGTCCACCTGCGCCCGCGGGAC
ATGTACATGCTCACCGTATCGAGCTGGGTGCTGGTATGCATCTTCGCCGCCCTGCCGTTCGTCCTGCAGCAG
CACATCAGCTACACCGATGCCTTCTTCGAAAGCATGTCGGGGATCACCGCCACCGGCTCCACCGTGCTCAGC
GGGCTGGACGACATGTCCCCGGGCATCCTCATCTGGCGCTCGCTGCTGCACTGGCTGGGCGGCATCGGCTTC
ATCGCCATGGCCGTGGCGATCCTGCCGATGCTGCGGATCGGCGGCATGCGCCTGTTCCAGACCGAATCCTCG
GACCGCTCGGAAAAGGTCCTGCCACGCTCGCACATGGTCGCCAAGTACATCGTCGCCACCTATGTCGGCATC
ACCCTGCTCGGCAGCCTGGCCTTCTGGGGCGCCGGCATGAGCCCGTTCGACGCGGTCAACCACGCCATGTCG
GCGATTTCCACCGGCGGTTTCTCCACCTCCGACCAATCCCTGGCCAAGTGGAAGCAGCCGGCGGTGCACTGG
ACCGCGGTAGTGGTGATGATCCTCGGCAGCCTGCCGTTCGCGCTCTACGTCGCCACCCTGCGCGGACACCGC
AAGGCGCTGATCAAGGACCAGCAGGTCCGCGGCCTGCTCGGCCTGCTGCTGGTGACCTGGCTGCTGATGGGC
accTGGTACGCGGTAACCTCCAAGCTGCCCTgGATGGATGCCTTCCGCATCGTCGCGGTGAATGTCACCTCG
GTGGTCACCACCACCGGCTTCGCCCTCGGCGACTACAGCATGTGGGGGCACTTCTCGATCATGCTGTTCTTC
TACCTCGGCTTCATCGGCGGTTGCTCCGGCTCTACCGCCGGCGGCATCAAGATCTTCCGCTTCCAGGTCGCC
TACACCCTGCTCAAGGCCAACCTCTACCAGTTGATCCACCCGCGCGCGGTGCTCAAGCAGAACTACAACGGC
CATCGGCTGGACGAGGAAATCGTCCGCTCGATCCTGACCTTCTCGTTCTTCTTCGCCATCGTCGTCTGCCTG
CTCGCCCTCTGCCTGTCGCTGATCGGCCTGGACTGGATGACCGCCCTCACCGGCGCCGCCAGCACCGTGGCC
GGCGTAGGCCCGGGGATGGGCCCCATCATCGGCCCCTCGGGCAACTTCGCCAGCCTGCCGGACGCCGCCAAG
TGGCTGCTGGCCGCCGGCATGCTGCTCGGCCGCCTGGAGATCATCACCGTGCTGGTCCTGCTGACCCCGGCG
TTCTGGCGCCACTGA
Translation:
MALPTLRILGFIIGIFLITLAASMLVPILTMLAFDRTEGIQAFIWSSVATFVTGLGLVLPGRPEHVHLRPRD
MYMLTVSSWVLVCIFAALPFVLQQHISYTDAFFESMSGITATGSTVLSGLDDMSPGILIWRSLLHWLGGIGF
IAMAVAILPMLRIGGMRLFQTESSDRSEKVLPRSHMVAKYIVATYVGITLLGSLAFWGAGMSPFDAVNHAMS
AISTGGFSTSDQSLAKWKQPAVHWTAVVVMILGSLPFALYVATLRGHRKALIKDQQVRGLLGLLLVTWLLMG
TWYAVTSKLPWMDAFRIVAVNVTSVVTTTGFALGDYSMWGHFSIMLFFYLGFIGGCSGSTAGGIKIFRFQVA
YTLLKANLYQLIHPRAVLKQNYNGHRLDEEIVRSILTFSFFFAIVVCLLALCLSLIGLDWMTALTGAASTVA
GVGPGMGPIIGPSGNFASLPDAAKWLLAAGMLLGRLEIITVLVLLTPAFWRH*
AnnotationID:873GeneID:864
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 08:08:03
Gene Name:trkH
Confidence Code:2
GeneProduct: potassium uptake protein TrkH
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02386,COG0168
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70081.1|AE016647_11 (AE016790) potassium uptake protein, TrkH family [Pseudomonas
           putida KT2440]
          Length = 484

 Score =  815 bits (2104), Expect = 0.0
 Identities = 391/484 (80%), Positives = 448/484 (92%)

>gb|AAN69547.1|AE016588_4 (AE016788) potassium uptake protein, TrkH family [Pseudomonas
           putida KT2440]
          Length = 484

 Score =  706 bits (1821), Expect = 0.0
 Identities = 336/483 (69%), Positives = 401/483 (83%)

>dbj|BAC61309.1| (AP005083) potassium uptake protein TrkH [Vibrio parahaemolyticus]
          Length = 481

 Score =  472 bits (1215), Expect = e-132
 Identities = 249/479 (51%), Positives = 318/479 (66%), Gaps = 3/479 (0%)






 
Structural Features:
pfam02386, TrkH, Cation transport protein.
CD-Length = 331 residues, 100.0% aligned
Score =  154 bits (391), Expect = 2e-38
Identities = 101/344 (29%), Positives = 148/344 (43%), Gaps = 28/344 (8%)

COG0168, TrkG, Trk-type K+ transport systems, membrane components 
[Inorganic ion transport and metabolism]
CD-Length = 499 residues,  97.6% aligned
Score =  334 bits (858), Expect = 1e-92
Identities = 191/495 (38%), Positives = 269/495 (54%), Gaps = 23/495 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1731




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18873




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32335




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:864_cdd.html


    		




 
URL:

















    ReferenceID:33977




  
Author/Investigator(s):
        Nakamura T, Yamamuro N, Stumpe S, Unemoto T, Bakker EP.





  
Title:
        Cloning of the trkAH gene cluster and characterization of the Trk K(+)-uptake system of Vibrio alginolyticus.




  
PubMed:
        9720051


MedLine:
        




    
Source:
Microbiology. 1998 Aug;144 ( Pt 8):2281-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9720051

















    ReferenceID:33978




  
Author/Investigator(s):
        Dosch DC, Helmer GL, Sutton SH, Salvacion FF, Epstein W.





  
Title:
        Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium.




  
PubMed:
        1987159


MedLine:
        




    
Source:
J Bacteriol. 1991 Jan;173(2):687-96.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1987159









           
Homologs By Global Alignment 

           

       



     
Gene ID:864
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1553
                           PA3210_tr
                           translation of PA3210
                           484
                           99.79
                           99.79
                           0
                           2474.0
     

     
     
55941
                           gb|AAG06598.1|AE004744_13
                           (AE004744) potassium uptake protein TrkH [Pseudomonas aeruginosa PAO1]
                           484
                           99.79
                           99.79
                           0
                           2474.0
     

     
     
55942
                           gb|AAN70081.1|AE016647_11
                           (AE016790) potassium uptake protein, TrkH family [Pseudomonas putida KT2440]
                           484
                           80.78
                           92.56
                           0
                           2104.0
     

     
     
55943
                           gb|AAO55321.1|
                           (AE016862) potassium uptake protein TrkH [Pseudomonas syringae pv. tomato str. DC3000]
                           484
                           79.95
                           91.11
                           0
                           2085.0
     

     
     
55944
                           gb|AAN69547.1|AE016588_4
                           (AE016788) potassium uptake protein, TrkH family [Pseudomonas putida KT2440]
                           484
                           69.42
                           82.85
                           0
                           1820.0
     

     






      

          
GC ORFID: 43866How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3210Glimmer Score: 

          
Start: 2978918Stop: 2980372

          
Length: 1455


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3210 translation of PA3210Homolog Bit Score 957.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7648 (ORF: BLASTX 813.0), t_PA3210 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCTTGCCGACCCTACGCATCCTCGGTTTCATCATCGGCATCTTCCTGATCACTCTGGCCGCCAGCATG 
CTGGTCCCGATCCTGACCATGCTCGCCTTCGACCGTACCGAAGGCATCCAGGCGTTCATCTGGTCCAGCGTC 
GCCACCTTCGTCACCGGCCTCGGCCTGGTCCTGCCGGGGCGTCCCGAGCATGTCCACCTGCGCCCGCGGGAC 
ATGTACATGCTCACCGTATCGAGCTGGGTGCTGGTATGCATCTTCGCCGCCCTGCCGTTCGTCCTGCAGCAG 
CACATCAGCTACACCGATGCCTTCTTCGAAAGCATGTCGGGGATCACCGCCACCGGCTCCACCGTGCTCAGC 
GGGCTGGACGACATGTCCCCGGGCATCCTCATCTGGCGCTCGCTGCTGCACTGGCTGGGCGGCATCGGCTTC 
ATCGCCATGGCCGTGGCGATCCTGCCGATGCTGCGGATCGGCGGCATGCGCCTGTTCCAGACCGAATCCTCG 
GACCGCTCGGAAAAGGTCCTGCCACGCTCGCACATGGTCGCCAAGTACATCGTCGCCACCTATGTCGGCATC 
ACCCTGCTCGGCAGCCTGGCCTTCTGGGGCGCCGGCATGAGCCCGTTCGACGCGGTCAACCACGCCATGTCG 
GCGATTTCCACCGGCGGTTTCTCCACCTCCGACCAATCCCTGGCCAAGTGGAAGCAGCCGGCGGTGCACTGG 
ACCGCGGTAGTGGTGATGATCCTCGGCAGCCTGCCGTTCGCGCTCTACGTCGCCACCCTGCGCGGACACCGC 
AAGGCGCTGATCAAGGACCAGCAGGTCCGCGGCCTGCTCGGCCTGCTGCTGGTGACCTGGCTGCTGATGGGC 
accTGGTACGCGGTAACCTCCAAGCTGCCCTgGATGGATGCCTTCCGCATCGTCGCGGTGAATGTCACCTCG 
GTGGTCACCACCACCGGCTTCGCCCTCGGCGACTACAGCATGTGGGGGCACTTCTCGATCATGCTGTTCTTC 
TACCTCGGCTTCATCGGCGGTTGCTCCGGCTCTACCGCCGGCGGCATCAAGATCTTCCGCTTCCAGGTCGCC 
TACACCCTGCTCAAGGCCAACCTCTACCAGTTGATCCACCCGCGCGCGGTGCTCAAGCAGAACTACAACGGC 
CATCGGCTGGACGAGGAAATCGTCCGCTCGATCCTGACCTTCTCGTTCTTCTTCGCCATCGTCGTCTGCCTG 
CTCGCCCTCTGCCTGTCGCTGATCGGCCTGGACTGGATGACCGCCCTCACCGGCGCCGCCAGCACCGTGGCC 
GGCGTAGGCCCGGGGATGGGCCCCATCATCGGCCCCTCGGGCAACTTCGCCAGCCTGCCGGACGCCGCCAAG 
TGGCTGCTGGCCGCCGGCATGCTGCTCGGCCGCCTGGAGATCATCACCGTGCTGGTCCTGCTGACCCCGGCG 
TTCTGGCGCCACTGA