ViewGene
Locus Name:
GeneID:
Location:

GeneID:862
TrivialName: PA14_05460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05460
PAO1 Ortholog Locus:PA0420
Sequence Length:1404
Protein Length:467
Start: 482987
Stop: 481584
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03809.1|AE004479_4 (AE004479) adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Pseudomonas aeruginosa PAO1]
Identities = 466/467 (99%), Positives = 467/467 (100%)
Sequence:
ATGGGCCTTAACGCCGACTGGATGCAGCGCGACCTGAACGTACTCTGGCATCCCTGTACCCAGATGAAAGAC
CACGAACGCCTGCCGGTGATCCCGATCCGCCGCGGCGAAGGCGTCTGGCTGGAGGATTTCGAAGGCAAGCGC
TACATCGACGCGGTCAGTTCCTGGTGGGTCAATGTGTTCGGCCACGCCAACCCGCGCATCAACCAGCGCATC
AAGGACCAGGTCGACCAGTTGGAGCACGTGATCCTCGCCGGATTCAGCCACCAGCCGGTGATCGAGCTGTCG
GAGCGACTGGTGAAGATCACCCCGCCGGGGCTCGACCGGGTGTTCTACGCCGACAGCGGCTCGGCGGGCATC
GAGGTCGCGCTGAAGATGAGCTACCACTTCTGGCTCAATAGCGGCAGGCCGCGCAAGAAGCGCTTCGTCACC
CTGACCAACAGCTACCACGGCGAAACCATCGCGGCGATGTCGGTGGGCGACGTGGCGCTGTTCACCGAAACC
TACAAGTCGCTGCTGCTGGACACCATCAAGGTGCCCAGCCCGGACTGCTTCCTGCGTCCGGACGGCATGTGC
TGGGAAGAACATTCGCGGAACATGTTCGCCCACATGGAGCGCACCCTGGCCGAAGGGCACGACGAGATCGCC
GCGGTCATCATCGAACCGCTGATCCAGGGCGCCGGCGGCATGCGCATGTACCATCCGGTCTACCTCAAGCTG
CTGCGCGAAGCCTGCGACCGCTATGGCGTGCACCTGATCCACGACGAGATCGCGGTGGGCTTCGGCCGCACC
GGGACGATGTTCGccTGCGAGCAGGCCGGCATCGCCCCGGATTTCCTCTGCCTGTCCAAGGCCCTCACCGGC
GGCTACCTGCCGATGTCCGCGGTGCTGACCAGCGAGACCATCTACCGGGGCTTCTACGATGACTACCAGACC
CTGCGCGCCTTCCTCCACTCGCACACCTATACCGGCAACCCGCTGGCCTGCGCCGCCGCCCTGGCGACCCTG
GACATCTTCGAGGAAGACAAGGTGATCGAGGCCAACCGCGCGCTGTCCACCCACATGGCCAGGGCCACCGCG
CACCTGGCCGACCACCCGCATGTCGCCGAGGTGCGCCAGACCGGGATGGTCCTGGCCATCGAAATGGTCCAG
GACAAGGCGTCCAGGACGCCCTACCCCTGGCAGGAGCGCCGCGGCCTGAAGGTCTTCCAGCATGGCCTGGAG
CGCGGCGCGCTGCTGCGCCCGCTGGGCAGCGTGGTGTATTTCCTGCCGCCCTACGTGATTACCCCGGAGCAG
ATCGACTTCCTCGCCGAGGTGGCCAGCGAAGGCATCGACATCGCCACCCGCGACGCGGTCAGCGTGGCGGTC
AGCGACTTCCACCCGGACCACCGCGATCCGGGCTGA
Translation:
MGLNADWMQRDLNVLWHPCTQMKDHERLPVIPIRRGEGVWLEDFEGKRYIDAVSSWWVNVFGHANPRINQRI
KDQVDQLEHVILAGFSHQPVIELSERLVKITPPGLDRVFYADSGSAGIEVALKMSYHFWLNSGRPRKKRFVT
LTNSYHGETIAAMSVGDVALFTETYKSLLLDTIKVPSPDCFLRPDGMCWEEHSRNMFAHMERTLAEGHDEIA
AVIIEPLIQGAGGMRMYHPVYLKLLREACDRYGVHLIHDEIAVGFGRTGTMFACEQAGIAPDFLCLSKALTG
GYLPMSAVLTSETIYRGFYDDYQTLRAFLHSHTYTGNPLACAAALATLDIFEEDKVIEANRALSTHMARATA
HLADHPHVAEVRQTGMVLAIEMVQDKASRTPYPWQERRGLKVFQHGLERGALLRPLGSVVYFLPPYVITPEQ
IDFLAEVASEGIDIATRDAVSVAVSDFHPDHRDPG*
AnnotationID:871GeneID:862
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 08:08:27
Gene Name:bioA
Confidence Code:2
GeneProduct:adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0161,pfam00202,COG0160
Secondary Functional Category(ies):
EC Number:2.6.1.62
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70550.1|AE016697_5 (AE016792) adenosylmethionine--8-amino-7-oxononanoate
           aminotransferase [Pseudomonas putida KT2440]
          Length = 468

 Score =  833 bits (2153), Expect = 0.0
 Identities = 401/468 (85%), Positives = 434/468 (92%), Gaps = 1/468 (0%)

>gb|AAO58502.1| (AE016874) adenosylmethionine--8-amino-7-oxononanoate
           aminotransferase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 468

 Score =  832 bits (2149), Expect = 0.0
 Identities = 398/468 (85%), Positives = 432/468 (92%), Gaps = 1/468 (0%)

>gb|AAO28049.1| (AE012553) adenosylmethionine-8-amino-7-oxononanoate
           aminotransferase [Xylella fastidiosa Temecula1]
          Length = 486

 Score =  502 bits (1292), Expect = e-141
 Identities = 247/460 (53%), Positives = 319/460 (69%), Gaps = 20/460 (4%)






 
Structural Features:
COG0161, BioA, Adenosylmethionine-8-amino-7-oxononanoate aminotransferase 
[Coenzyme metabolism]
CD-Length = 449 residues,  99.8% aligned
Score =  567 bits (1463), Expect = 1e-162
Identities = 217/453 (47%), Positives = 293/453 (64%), Gaps = 9/453 (1%)

pfam00202, Aminotran_3, Aminotransferase class-III.
CD-Length = 402 residues,  98.0% aligned
Score =  372 bits (956), Expect = 6e-104
Identities = 143/418 (34%), Positives = 217/418 (51%), Gaps = 26/418 (6%)

COG0160, GabT, 4-aminobutyrate aminotransferase and related aminotransferases 
[Amino acid transport and metabolism]
CD-Length = 447 residues,  96.2% aligned
Score =  305 bits (783), Expect = 7e-84
Identities = 152/454 (33%), Positives = 243/454 (53%), Gaps = 33/454 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1727




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18869




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33747




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:862_cdd.html


    		




 
URL:

















    ReferenceID:35365




  
Author/Investigator(s):
        Kiyasu T, Nagahashi Y, Hoshino T.





  
Title:
        Cloning and characterization of biotin biosynthetic genes of Kurthia sp.




  
PubMed:
        11255013


MedLine:
        




    
Source:
Gene. 2001 Mar 7;265(1-2):103-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11255013

















    ReferenceID:35366




  
Author/Investigator(s):
        Sakurai N, Akatsuka H, Kawai E, Imai Y, Komatsubara S.





  
Title:
        Complete sequence and organization of the Serratia marcescens biotin operon.




  
PubMed:
        8969526


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3295-303.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969526

















    ReferenceID:35367




  
Author/Investigator(s):
        Wu CH, Chen HY, Shiuan D.





  
Title:
        Isolation and characterization of the Erwinia herbicola bio operon and the sequences of the bioA and bioB genes.




  
PubMed:
        8890743


MedLine:
        




    
Source:
Gene. 1996 Oct 3;174(2):251-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8890743

















    ReferenceID:35368




  
Author/Investigator(s):
        Hatakeyama K, Hohama K, Vertes AA, Kobayashi M, Kurusu Y, Yukawa H.





  
Title:
        Genomic organization of the biotin biosynthetic genes of coryneform bacteria: cloning and sequencing of the bioA-bioD genes from Brevibacterium flavum.




  
PubMed:
        8161820


MedLine:
        




    
Source:
DNA Seq. 1993;4(3):177-84.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8161820

















    ReferenceID:35369




  
Author/Investigator(s):
        Otsuka AJ, Buoncristiani MR, Howard PK, Flamm J, Johnson C, Yamamoto R, Uchida K, Cook C, Ruppert J, Matsuzaki J.





  
Title:
        The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon.




  
PubMed:
        3058702


MedLine:
        




    
Source:
J Biol Chem. 1988 Dec 25;263(36):19577-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3058702









           
Homologs By Global Alignment 

           

       



     
Gene ID:862
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1550
                           PA0420_tr
                           translation of PA0420
                           467
                           99.78
                           99.99
                           0
                           2479.0
     

     
     
55856
                           gb|AAG03809.1|AE004479_4
                           (AE004479) adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Pseudomonas aeruginosa PAO1]
                           467
                           99.78
                           99.99
                           0
                           2479.0
     

     
     
55857
                           gb|AAN70550.1|AE016697_5
                           (AE016792) adenosylmethionine--8-amino-7-oxononanoate aminotransferase [Pseudomonas putida KT2440]
                           468
                           85.68
                           92.73
                           1
                           2155.0
     

     
     
55858
                           gb|AAO58502.1|
                           (AE016874) adenosylmethionine--8-amino-7-oxononanoate aminotransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           468
                           85.04
                           92.30
                           1
                           2151.0
     

     
     
55867
                           emb|CAE09391.1|
                           (BX571657) OXONONANOATE AMINOTRANSFERASE [Wolinella succinogenes]
                           473
                           50.31
                           65.75
                           47
                           1184.0
     

     






      

          
GC ORFID: 43864How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0420Glimmer Score: 

          
Start: 6284329Stop: 6282926

          
Length: 1404


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0420 translation of PA0420Homolog Bit Score 959.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3739 (ORF: BLASTX 832.0), t_PA0420 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCCTTAACGCCGACTGGATGCAGCGCGACCTGAACGTACTCTGGCATCCCTGTACCCAGATGAAAGAC 
CACGAACGCCTGCCGGTGATCCCGATCCGCCGCGGCGAAGGCGTCTGGCTGGAGGATTTCGAAGGCAAGCGC 
TACATCGACGCGGTCAGTTCCTGGTGGGTCAATGTGTTCGGCCACGCCAACCCGCGCATCAACCAGCGCATC 
AAGGACCAGGTCGACCAGTTGGAGCACGTGATCCTCGCCGGATTCAGCCACCAGCCGGTGATCGAGCTGTCG 
GAGCGACTGGTGAAGATCACCCCGCCGGGGCTCGACCGGGTGTTCTACGCCGACAGCGGCTCGGCGGGCATC 
GAGGTCGCGCTGAAGATGAGCTACCACTTCTGGCTCAATAGCGGCAGGCCGCGCAAGAAGCGCTTCGTCACC 
CTGACCAACAGCTACCACGGCGAAACCATCGCGGCGATGTCGGTGGGCGACGTGGCGCTGTTCACCGAAACC 
TACAAGTCGCTGCTGCTGGACACCATCAAGGTGCCCAGCCCGGACTGCTTCCTGCGTCCGGACGGCATGTGC 
TGGGAAGAACATTCGCGGAACATGTTCGCCCACATGGAGCGCACCCTGGCCGAAGGGCACGACGAGATCGCC 
GCGGTCATCATCGAACCGCTGATCCAGGGCGCCGGCGGCATGCGCATGTACCATCCGGTCTACCTCAAGCTG 
CTGCGCGAAGCCTGCGACCGCTATGGCGTGCACCTGATCCACGACGAGATCGCGGTGGGCTTCGGCCGCACC 
GGGACGATGTTCGccTGCGAGCAGGCCGGCATCGCCCCGGATTTCCTCTGCCTGTCCAAGGCCCTCACCGGC 
GGCTACCTGCCGATGTCCGCGGTGCTGACCAGCGAGACCATCTACCGGGGCTTCTACGATGACTACCAGACC 
CTGCGCGCCTTCCTCCACTCGCACACCTATACCGGCAACCCGCTGGCCTGCGCCGCCGCCCTGGCGACCCTG 
GACATCTTCGAGGAAGACAAGGTGATCGAGGCCAACCGCGCGCTGTCCACCCACATGGCCAGGGCCACCGCG 
CACCTGGCCGACCACCCGCATGTCGCCGAGGTGCGCCAGACCGGGATGGTCCTGGCCATCGAAATGGTCCAG 
GACAAGGCGTCCAGGACGCCCTACCCCTGGCAGGAGCGCCGCGGCCTGAAGGTCTTCCAGCATGGCCTGGAG 
CGCGGCGCGCTGCTGCGCCCGCTGGGCAGCGTGGTGTATTTCCTGCCGCCCTACGTGATTACCCCGGAGCAG 
ATCGACTTCCTCGCCGAGGTGGCCAGCGAAGGCATCGACATCGCCACCCGCGACGCGGTCAGCGTGGCGGTC 
AGCGACTTCCACCCGGACCACCGCGATCCGGGCTGA