ViewGene
Locus Name:
GeneID:
Location:

GeneID:860
TrivialName: PA14_09210
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_09210
PAO1 Ortholog Locus:PA4231
Sequence Length:1431
Protein Length:476
Start: 781697
Stop: 780267
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07619.1|AE004840_5 (AE004840) salicylate biosynthesis isochorismate synthase [Pseudomonas aeruginosa PAO1]
Identities = 473/476 (99%), Positives = 474/476 (99%)
Sequence:
ATGAGCCGGCTGGCGCCCCTGAGCCAGTGCCTGCACGCCTTGCGCGGCACCTTCGAGCGCGCCATCGGCCAG
GCGCAGGCGCTCGATCGTCCGGTGCTGGTGGCGGCGTCGTTCGAGATCGACCCATTGGACCCGCTGCAGGTA
TTCGGTGCCTGGGACGACCGGCAAACGCCCTGCCTGTACTGGGAACAGCCCGAGCTGGCGTTCTTCGCCTGG
GGCTGCGCCCTGGAGCTGCAAGGCCACGGCGAACAGCGCTTCGCCCGGATCGAGGAAAACTGGCAATTGCTC
TGCGCCGACGCCGTGGTCGAGGGCCCGCTGGCGCCGCGCCTGTGCGGCGGATTCCGCTTCGATCCGCGCGGC
CCGCGCGAGGAACACTGGCAAGCCTTCGCCGATGCCAGCCTGATGCTCGCCGGCATCACCGTGCTGCGCGAG
GGCGAACGCTACCGGGTACTCTGCCAACACCTGGCCAAGCCCGGCGAAGATGCCCTGGCCCTGGCCGCCTAC
CACTGCTCGGCGCTACTGCGCCTGAGGCAGCCGGCCAGGCGCCGGCCCTCGGGGCCGACCGCTGGCGCGCAG
GGCGACGCTTCGGCGCTGGAGCGCAGGCAATGGGAAGCCAAGGTGAGCGACGCGGTAAGCAGTGTCCGCCAG
GGACGCTTCGGCAAGGTCGTGCTGGCCCGCACCCAGGCCCGGCCTCTCGGCGACATCGAGCCGTGGCAGGTC
ATCGAACACCTGCGCCTACAACATGCCGACGCCCAGCTGTTCGCCTGTCGCCGCGGCAACGCCTGCTTCCTC
GGCGCCTCCCCGGAACGCCTGGTCCGCATTCGCGCCGGCGAGGCGCTCACCCATGCCCTGGCCGGGACCATC
GCCCGCGGCGGCGATGCCCAGGAAGATGCGCGGCTCGGACAGGCCCTGCTGGACAGCGCCAAGGACAGGCAC
GAACACCAGTTGGTGGTGGAGGCGATCCGTACGGCCCTGGAACCCTTCAGCGAGGCGCTGGAAATCCCCGAT
GCGCCTGGCCTGAAACGACTGGCGCGAGTCCAGCACCTGAACACGCCGATCCGCGCCCGCCTCGCCGACGCA
GGCGGCATCCTGCGGCTGCTGCAGGCGCTGCATCCGACCCCCGCGGTGGGCGGCTACCCACGCAGCGCGGCG
CTGGACTACATCCGCCAGCACGAAGGGATGGATCGTGGCTGGTACGCCGCGCCGCTGGGCTGGCTCGACGGC
GAAGGCAACGGCGATTTCCTGGTGGCGCTGCGCTCGGCCCTGCTCACGCCGGGCCGGGGCTACCTGTTCGCC
GGCTGCGGTCTGGTAGGCGATTCGGAACCGGCCCACGAGTATCGCGAAACCTGCCTTAAGCTCAGTGCCATG
CGGGACGCTCTGTCCGCCATAGGCGGCCTGGACGAAGTGCCCTTGCAGCGCGGCGTCGCCTGA
Translation:
MSRLAPLSQCLHALRGTFERAIGQAQALDRPVLVAASFEIDPLDPLQVFGAWDDRQTPCLYWEQPELAFFAW
GCALELQGHGEQRFARIEENWQLLCADAVVEGPLAPRLCGGFRFDPRGPREEHWQAFADASLMLAGITVLRE
GERYRVLCQHLAKPGEDALALAAYHCSALLRLRQPARRRPSGPTAGAQGDASALERRQWEAKVSDAVSSVRQ
GRFGKVVLARTQARPLGDIEPWQVIEHLRLQHADAQLFACRRGNACFLGASPERLVRIRAGEALTHALAGTI
ARGGDAQEDARLGQALLDSAKDRHEHQLVVEAIRTALEPFSEALEIPDAPGLKRLARVQHLNTPIRARLADA
GGILRLLQALHPTPAVGGYPRSAALDYIRQHEGMDRGWYAAPLGWLDGEGNGDFLVALRSALLTPGRGYLFA
GCGLVGDSEPAHEYRETCLKLSAMRDALSAIGGLDEVPLQRGVA*
AnnotationID:869GeneID:860
AnnotatorUID: diggins
Modification Date/Time: 2005-06-06 11:11:08
Gene Name:pchA
Confidence Code:1
GeneProduct:salicylate biosynthesis isochorismate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1169,pfam00425
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:5.4.4.2
Status:ACTIVE
Pathway:
Siderophore biosynthesis; salicylate biosynthesis.
Homology:
>gb|AAO56099.1| (AE016865) isochorismate synthase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 469

 Score =  303 bits (777), Expect = 1e-81
 Identities = 188/465 (40%), Positives = 250/465 (53%), Gaps = 23/465 (4%)
Structural Features:
COG1169, MenF, Isochorismate synthase 
[Coenzyme metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 423 residues, 100.0% aligned
Score =  397 bits (1020), Expect = 2e-111
Identities = 178/464 (38%), Positives = 227/464 (48%), Gaps = 47/464 (10%)

pfam00425, Chorismate_bind, chorismate binding enzyme.
CD-Length = 263 residues, 100.0% aligned
Score =  243 bits (621), Expect = 4e-65
Identities = 93/264 (35%), Positives = 140/264 (53%), Gaps = 3/264 (1%)
Genomic Context:






   
Comment:


















    ReferenceID:1723




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18865




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36929




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:860_cdd.html


    		




 
URL:

















    ReferenceID:37017




  
Author/Investigator(s):
        Serino L, Reimmann C, Baur H, Beyeler M, Visca P, Haas D.





  
Title:
        Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.




  
PubMed:
        7500944


MedLine:
        




    
Source:
Mol Gen Genet. 1995 Nov 15;249(2):217-28.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=96086939&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:860
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1548
                           PA4231_tr
                           translation of PA4231
                           476
                           99.36
                           99.57
                           0
                           2480.0
     

     
     
55807
                           gb|AAG07619.1|AE004840_5
                           (AE004840) salicylate biosynthesis isochorismate synthase [Pseudomonas aeruginosa PAO1]
                           476
                           99.36
                           99.57
                           0
                           2480.0
     

     
     
55808
                           emb|CAA57969.1|
                           (X82644) pchA [Pseudomonas aeruginosa]
                           476
                           99.36
                           99.57
                           0
                           2480.0
     

     
     
55809
                           gb|AAO56099.1|
                           (AE016865) isochorismate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           499
                           39.27
                           51.90
                           53
                           789.0
     

     
     
55811
                           dbj|BAC08765.1|
                           (AP005373) isochorismate synthase [Thermosynechococcus elongatus BP-1]
                           506
                           30.03
                           43.28
                           78
                           477.0
     

     






      

          
GC ORFID: 43862How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4231Glimmer Score: 

          
Start: 4172240Stop: 4173670

          
Length: 1431


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4231 translation of PA4231Homolog Bit Score 959.0

          
Other Homologs:  t_PA4231 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGGCTGGCGCCCCTGAGCCAGTGCCTGCACGCCTTGCGCGGCACCTTCGAGCGCGCCATCGGCCAG 
GCGCAGGCGCTCGATCGTCCGGTGCTGGTGGCGGCGTCGTTCGAGATCGACCCATTGGACCCGCTGCAGGTA 
TTCGGTGCCTGGGACGACCGGCAAACGCCCTGCCTGTACTGGGAACAGCCCGAGCTGGCGTTCTTCGCCTGG 
GGCTGCGCCCTGGAGCTGCAAGGCCACGGCGAACAGCGCTTCGCCCGGATCGAGGAAAACTGGCAATTGCTC 
TGCGCCGACGCCGTGGTCGAGGGCCCGCTGGCGCCGCGCCTGTGCGGCGGATTCCGCTTCGATCCGCGCGGC 
CCGCGCGAGGAACACTGGCAAGCCTTCGCCGATGCCAGCCTGATGCTCGCCGGCATCACCGTGCTGCGCGAG 
GGCGAACGCTACCGGGTACTCTGCCAACACCTGGCCAAGCCCGGCGAAGATGCCCTGGCCCTGGCCGCCTAC 
CACTGCTCGGCGCTACTGCGCCTGAGGCAGCCGGCCAGGCGCCGGCCCTCGGGGCCGACCGCTGGCGCGCAG 
GGCGACGCTTCGGCGCTGGAGCGCAGGCAATGGGAAGCCAAGGTGAGCGACGCGGTAAGCAGTGTCCGCCAG 
GGACGCTTCGGCAAGGTCGTGCTGGCCCGCACCCAGGCCCGGCCTCTCGGCGACATCGAGCCGTGGCAGGTC 
ATCGAACACCTGCGCCTACAACATGCCGACGCCCAGCTGTTCGCCTGTCGCCGCGGCAACGCCTGCTTCCTC 
GGCGCCTCCCCGGAACGCCTGGTCCGCATTCGCGCCGGCGAGGCGCTCACCCATGCCCTGGCCGGGACCATC 
GCCCGCGGCGGCGATGCCCAGGAAGATGCGCGGCTCGGACAGGCCCTGCTGGACAGCGCCAAGGACAGGCAC 
GAACACCAGTTGGTGGTGGAGGCGATCCGTACGGCCCTGGAACCCTTCAGCGAGGCGCTGGAAATCCCCGAT 
GCGCCTGGCCTGAAACGACTGGCGCGAGTCCAGCACCTGAACACGCCGATCCGCGCCCGCCTCGCCGACGCA 
GGCGGCATCCTGCGGCTGCTGCAGGCGCTGCATCCGACCCCCGCGGTGGGCGGCTACCCACGCAGCGCGGCG 
CTGGACTACATCCGCCAGCACGAAGGGATGGATCGTGGCTGGTACGCCGCGCCGCTGGGCTGGCTCGACGGC 
GAAGGCAACGGCGATTTCCTGGTGGCGCTGCGCTCGGCCCTGCTCACGCCGGGCCGGGGCTACCTGTTCGCC 
GGCTGCGGTCTGGTAGGCGATTCGGAACCGGCCCACGAGTATCGCGAAACCTGCCTTAAGCTCAGTGCCATG 
CGGGACGCTCTGTCCGCCATAGGCGGCCTGGACGAAGTGCCCTTGCAGCGCGGCGTCGCCTGA