ViewGene
Locus Name:
GeneID:
Location:

GeneID:859
TrivialName: PA14_14470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14470
PAO1 Ortholog Locus:PA3831
Sequence Length:1488
Protein Length:495
Start: 1236846
Stop: 1235359
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07218.1|AE004800_7 (AE004800) leucine aminopeptidase [Pseudomonas aeruginosa PAO1]
Identities = 492/495 (99%), Positives = 493/495 (99%)
Sequence:
ATGGAATTCCTTGTTAAAAGCGTACGCCCGGAAACCCTGAAAACCGCCACCCTGGTGCTCGCCGTAGGCGAA
GGCCGCAAGCTCGGCGCCAGCGCCAAGGCGGTCGACGACGCCACCGGCGGCGCCATCAGCGCAGTGCTCAAG
CGTGGCGACCTGGCCGGCAAGGTCGGCCAGACCCTGCTCCTGCAGAGCCTGCCCAATCTCAAGGCCGAGCGC
GTGCTGCTGGTCGGCGCCGGCAAGGAGCGCGAACTGGGCGACCGCCAGTACCGCAAGCTGGCCTCCGCCGTG
CTGTCGACCCTCAAGGGCCTGGCCGGCGCCGATGCCGCGCTAGCCCTCGGCGATCTCGCGGTGAAAGGCCGC
GGTGCCCATGCGAAGGCGCGTCTGCTGGTGGAAACCCTGGCCGACGGCCTGTACGTGTTCGATCGCTACAAG
AGCCAGAAAGCCGAGCCGCTGAAACTGAAGAAGCTGACCCTGCTCGCCGACAAGGCCGACAGCGCCGCCGTC
GAGCAAGGCAGCAAGGAAGCCCAGGCCATCGCCAATGGCATGGCCCTGACCCGCGATCTCGGCAACCTGCCG
CCGAACGTCTGCCATCCGACGTTCCTCGGCGAGCAGGCCAAGGGCCTGGCCAAGGAGTTCAAGAGCCTGAAG
GTCGAGGTCCTCGACGAGAAGAAACTGCGCGAGCTGGGCATGGGCTCGTTCCTCGCCGTCGCCCAGGGCAGC
GACCAGCCGCCGCGGCTGATCATCCTGCAATACAACGGCGCGAAGAAGGACCAGGCCCCGCACGTGCTGGTC
GGCAAGGGCATCACCTTCGACACCGGCGGCATCAGCCTCAAGCCGGGCCTGGGCATGGACGAGATGAAGTTC
GACATGTGCGGCGCCGCCAGCGTCTTCGGCACCTTCCGCGCGGTGCTCGAACTGCAGTTGCCGATCAACCTG
GTCGGCCTGCTCGCCTGCGCCGAGAACATGCCCAGCGGCGGAGCCACCCGTCCGGGGGACATCGTCACCACC
ATGAGCGGGCAGACCGTCGAGATCCTCAACACCGACGCCGAAGGGCGCCTGGTGCTGTGCGACGCACTGACC
TACGCCGAGCGCTTCAAGCCGCAGTCGGTGATCGACATCGCCACCCTCACCGGCGCCTGCATCGTCGCCCTG
GGCAGCAACACCTCGGGCCTTATGGGCAACAACGAAGCGCTGGTCAGGCAACTGCTCAAGGCCGGCGAGTTC
GCCGACGACCGCGCCTGGCAATTGCCGCTGTTCGACGAATACCAGGAACAGCTGGACAGCCCGTTCGCCGAC
ATCGCCAACATCGGCGGGCCGAAGGCCGGCACCATCACCGCCGGCTGCTTCCTCTCGCGCTTCGCCAAGAAA
TACCACTGGGCGCACCTGGACATCGCCGGCACCGCCTGGATCAGCGGCGGCAAGGACAAAGGCGCCACCGGC
CGCCCGGTTCCGTTGTTGACCCAGTACCTGCTGGAACGGGCCAAGTGA
Translation:
MEFLVKSVRPETLKTATLVLAVGEGRKLGASAKAVDDATGGAISAVLKRGDLAGKVGQTLLLQSLPNLKAER
VLLVGAGKERELGDRQYRKLASAVLSTLKGLAGADAALALGDLAVKGRGAHAKARLLVETLADGLYVFDRYK
SQKAEPLKLKKLTLLADKADSAAVEQGSKEAQAIANGMALTRDLGNLPPNVCHPTFLGEQAKGLAKEFKSLK
VEVLDEKKLRELGMGSFLAVAQGSDQPPRLIILQYNGAKKDQAPHVLVGKGITFDTGGISLKPGLGMDEMKF
DMCGAASVFGTFRAVLELQLPINLVGLLACAENMPSGGATRPGDIVTTMSGQTVEILNTDAEGRLVLCDALT
YAERFKPQSVIDIATLTGACIVALGSNTSGLMGNNEALVRQLLKAGEFADDRAWQLPLFDEYQEQLDSPFAD
IANIGGPKAGTITAGCFLSRFAKKYHWAHLDIAGTAWISGGKDKGATGRPVPLLTQYLLERAK*
AnnotationID:868GeneID:859
AnnotatorUID: diggins
Modification Date/Time: 2005-06-14 10:10:51
Gene Name:pepA
Confidence Code:1
GeneProduct:leucine aminopeptidase
Cell Localization:(1) Cytoplasmic
Synonyms:phpA
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:cytosol aminopeptidase
Functional Category Confidence Code:5
COGs:COG0260,pfam00883,KOG2597
Secondary Functional Category(ies):
EC Number:3.4.11.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66605.1|AE016288_4 (AE016777) cytosol aminopeptidase [Pseudomonas putida KT2440]
          Length = 497

 Score =  767 bits (1981), Expect = 0.0
 Identities = 394/495 (79%), Positives = 428/495 (86%), Gaps = 1/495 (0%)

>emb|CAA09054.1| (AJ010261) leucine aminopeptidase [Pseudomonas putida]
          Length = 497

 Score =  767 bits (1980), Expect = 0.0
 Identities = 394/495 (79%), Positives = 428/495 (86%), Gaps = 1/495 (0%)







 
Structural Features:
COG0260, PepB, Leucyl aminopeptidase 
[Amino acid transport and metabolism]
CD-Length = 485 residues, 100.0% aligned
Score =  552 bits (1424), Expect = 6e-158
Identities = 254/498 (51%), Positives = 323/498 (64%), Gaps = 16/498 (3%)

pfam00883, Peptidase_M17, Cytosol aminopeptidase family, 
catalytic domain. 
CD-Length = 310 residues,  99.4% aligned
Score =  390 bits (1003), Expect = 4e-109
Identities = 170/309 (55%), Positives = 222/309 (71%), Gaps = 5/309 (1%)

KOG2597, Predicted aminopeptidase of the M17 family 
CD-Length = 513 residues,  98.1% aligned
Score =  346 bits (889), Expect = 7e-96
Identities = 194/513 (37%), Positives = 259/513 (50%), Gaps = 31/513 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1721




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18863




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31726




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:859_cdd.html


    		




 
URL:

















    ReferenceID:34316




  
Author/Investigator(s):
        Hauser AR, Kang PJ, Engel JN.





  
Title:
        PepA, a secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence.




  
PubMed:
        9515706


MedLine:
        




    
Source:
Mol Microbiol. 1998 Feb;27(4):807-18.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9515706

















    ReferenceID:34317




  
Author/Investigator(s):
        Woolwine SC, Wozniak DJ.





  
Title:
        Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.




  
PubMed:
        9864319


MedLine:
        




    
Source:
J Bacteriol. 1999 Jan;181(1):107-16.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9864319









           
Homologs By Global Alignment 

           

       



     
Gene ID:859
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1547
                           PA3831_tr
                           translation of PA3831
                           495
                           99.39
                           99.59
                           0
                           2479.0
     

     
     
55726
                           gb|AAG07218.1|AE004800_7
                           (AE004800) leucine aminopeptidase [Pseudomonas aeruginosa PAO1]
                           495
                           99.39
                           99.59
                           0
                           2479.0
     

     
     
55727
                           gb|AAD04821.1|
                           (AF054622) PhpA [Pseudomonas aeruginosa]
                           495
                           99.39
                           99.59
                           0
                           2479.0
     

     
     
55728
                           gb|AAN66605.1|AE016288_4
                           (AE016777) cytosol aminopeptidase [Pseudomonas putida KT2440]
                           497
                           79.27
                           86.11
                           2
                           1981.0
     

     
     
55729
                           emb|CAA09054.1|
                           (AJ010261) leucine aminopeptidase [Pseudomonas putida]
                           497
                           79.27
                           86.11
                           2
                           1980.0
     

     






      

          
GC ORFID: 43861How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3831Glimmer Score: 

          
Start: 3717092Stop: 3718579

          
Length: 1488


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3831 translation of PA3831Homolog Bit Score 959.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4970 (ORF: BLASTX 766.0), t_PA3831 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAATTCCTTGTTAAAAGCGTACGCCCGGAAACCCTGAAAACCGCCACCCTGGTGCTCGCCGTAGGCGAA 
GGCCGCAAGCTCGGCGCCAGCGCCAAGGCGGTCGACGACGCCACCGGCGGCGCCATCAGCGCAGTGCTCAAG 
CGTGGCGACCTGGCCGGCAAGGTCGGCCAGACCCTGCTCCTGCAGAGCCTGCCCAATCTCAAGGCCGAGCGC 
GTGCTGCTGGTCGGCGCCGGCAAGGAGCGCGAACTGGGCGACCGCCAGTACCGCAAGCTGGCCTCCGCCGTG 
CTGTCGACCCTCAAGGGCCTGGCCGGCGCCGATGCCGCGCTAGCCCTCGGCGATCTCGCGGTGAAAGGCCGC 
GGTGCCCATGCGAAGGCGCGTCTGCTGGTGGAAACCCTGGCCGACGGCCTGTACGTGTTCGATCGCTACAAG 
AGCCAGAAAGCCGAGCCGCTGAAACTGAAGAAGCTGACCCTGCTCGCCGACAAGGCCGACAGCGCCGCCGTC 
GAGCAAGGCAGCAAGGAAGCCCAGGCCATCGCCAATGGCATGGCCCTGACCCGCGATCTCGGCAACCTGCCG 
CCGAACGTCTGCCATCCGACGTTCCTCGGCGAGCAGGCCAAGGGCCTGGCCAAGGAGTTCAAGAGCCTGAAG 
GTCGAGGTCCTCGACGAGAAGAAACTGCGCGAGCTGGGCATGGGCTCGTTCCTCGCCGTCGCCCAGGGCAGC 
GACCAGCCGCCGCGGCTGATCATCCTGCAATACAACGGCGCGAAGAAGGACCAGGCCCCGCACGTGCTGGTC 
GGCAAGGGCATCACCTTCGACACCGGCGGCATCAGCCTCAAGCCGGGCCTGGGCATGGACGAGATGAAGTTC 
GACATGTGCGGCGCCGCCAGCGTCTTCGGCACCTTCCGCGCGGTGCTCGAACTGCAGTTGCCGATCAACCTG 
GTCGGCCTGCTCGCCTGCGCCGAGAACATGCCCAGCGGCGGAGCCACCCGTCCGGGGGACATCGTCACCACC 
ATGAGCGGGCAGACCGTCGAGATCCTCAACACCGACGCCGAAGGGCGCCTGGTGCTGTGCGACGCACTGACC 
TACGCCGAGCGCTTCAAGCCGCAGTCGGTGATCGACATCGCCACCCTCACCGGCGCCTGCATCGTCGCCCTG 
GGCAGCAACACCTCGGGCCTTATGGGCAACAACGAAGCGCTGGTCAGGCAACTGCTCAAGGCCGGCGAGTTC 
GCCGACGACCGCGCCTGGCAATTGCCGCTGTTCGACGAATACCAGGAACAGCTGGACAGCCCGTTCGCCGAC 
ATCGCCAACATCGGCGGGCCGAAGGCCGGCACCATCACCGCCGGCTGCTTCCTCTCGCGCTTCGCCAAGAAA 
TACCACTGGGCGCACCTGGACATCGCCGGCACCGCCTGGATCAGCGGCGGCAAGGACAAAGGCGCCACCGGC 
CGCCCGGTTCCGTTGTTGACCCAGTACCTGCTGGAACGGGCCAAGTGA