ViewGene
Locus Name:
GeneID:
Location:

GeneID:857
TrivialName: PA14_70950
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70950
PAO1 Ortholog Locus:PA5373
Sequence Length:1473
Protein Length:490
Start: 6323576
Stop: 6322104
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08758.1|AE004950_1 (AE004950) betaine aldehyde dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 487/490 (99%), Positives = 487/490 (99%)
Sequence:
ATGGCCCGATTCGAAGAACAGAAGCTCTACATTGGCGGTCGCTACGTGGAGGCCAGCAGTGGCGCCACCTTC
GAGACCATCAACCCGGCCAACGGCGAAGTGCTCGCCAAGGTCCAGCGGGCTTCCAGGGAAGACGTCGAGCGG
GCCGTGCAGAGCGCCGTCGAGGGGCAGAAGGTGTGGGCGGCGATGACCGCCATGCAGCGCTCGCGCATCCTC
CGTCGCGCCGTCGACATCCTCCGCGAGCGCAACGACGAACTGGCCGCCCTGGAAACCCTGGATACCGGCAAG
CCGCTGGCGGAAACCCGCTCGGTGGACATCGTCACCGGCGCCGACGTGCTCGAGTACTACGCCGGCCTGGTC
CCGGCCATCGAAGGCGAGCAGATTCCCCTGCGCGAAACCAGTTTCGTCTACACCCGCCGCGAGCCGCTGGGC
GTGGTCGCCGGCATTGGCGCCTGGAACTACCCGGTGCAGATCGCCCTGTGGAAATCCGCCCCGGCCCTCGCT
GCCGGCAACGCGATGATCTTCAAGCCCAGCGAAGTCACCCCGCTGACCACCCTCAAGCTGGCCGAGATCTAC
ACCGAGGCCGGCGTGCCGGACGGCGTGTTCAACGTGCTCACCGGCAGCGGACGCGAAGTCGGCCAGTGGCTG
ACCGAGCACCCGCTGATCGAGAAGATCTCCTTCACCGGCGGCACCTCCACCGGCAAGAAAGTCATGGCCAGC
GCTTCCAGCTCGTCGCTGAAGGAAGTGACCATGGAGCTGGGCGGCAAGTCCCCGCTGATCATCTTCCCCGAC
GCAGACCTCGACCGCGCCGCCGACATCGCCGTCATGGCCAACTTCTTCAGCTCCGGCCAGGTCTGCACCAAT
GGCACCCGGGTGTTCATCCACCGTTCCCAGCAGGCCCGCTTCGAAGCCAAGGTACTGGAGCGCGTGCAGCGC
ATCCGTCTCGGCGACCCGCAGGACGAGAACACCAACTTCGGCCCGCTGGTCAGCTTCCCGCACATGGAAAGC
GTGCTCGGCTACATCGAGTCCGGCAAGGCGCAGAAGGCTCGCCTGCTGTGCGGCGGCGAGCGTGTGACCGAT
GGAGCGTTCGGCAATGGCGCCTACGTCGCGCCGACCGTGTTCACCGATTGCAGCGACGACATGACCATCGTC
CGCGAGGAAATCTTCGGCCCGGTGATGAGCATCCTGGTCTATGACGACGAAGACGAGGCGATCCGCCGCGCC
AACGATACCGAGTACGGCCTCGCCGCCGGCGTCGTGACCCAGGACCTGGCCCGCGCCCACCGCGCCATCCAT
CGCCTGGAAGCCGGCATCTGCTGGATCAATACCTGGGGCGAGTCGCCGGCGGAAATGCCGGTCGGCGGTTAC
AAGCAATCCGGTGTCGGTCGCGAGAACGGTCTCACCACCCTGGCTCATTACACTCGCATCAAGTCCGTGCAA
GTGGAGCTGGGCGACTACGCCTCGGTGTTCTGA
Translation:
MARFEEQKLYIGGRYVEASSGATFETINPANGEVLAKVQRASREDVERAVQSAVEGQKVWAAMTAMQRSRIL
RRAVDILRERNDELAALETLDTGKPLAETRSVDIVTGADVLEYYAGLVPAIEGEQIPLRETSFVYTRREPLG
VVAGIGAWNYPVQIALWKSAPALAAGNAMIFKPSEVTPLTTLKLAEIYTEAGVPDGVFNVLTGSGREVGQWL
TEHPLIEKISFTGGTSTGKKVMASASSSSLKEVTMELGGKSPLIIFPDADLDRAADIAVMANFFSSGQVCTN
GTRVFIHRSQQARFEAKVLERVQRIRLGDPQDENTNFGPLVSFPHMESVLGYIESGKAQKARLLCGGERVTD
GAFGNGAYVAPTVFTDCSDDMTIVREEIFGPVMSILVYDDEDEAIRRANDTEYGLAAGVVTQDLARAHRAIH
RLEAGICWINTWGESPAEMPVGGYKQSGVGRENGLTTLAHYTRIKSVQVELGDYASVF*
AnnotationID:866GeneID:857
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 20:08:52
Gene Name:betB
Confidence Code:2
GeneProduct:betaine aldehyde dehydrogenase
Cell Localization:()
Synonyms:badH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00171,COG1012
Secondary Functional Category(ies):adaptation, protection
EC Number:1.2.1.8
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53985.1| (AE016857) betaine aldehyde dehydrogenase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 490

 Score =  819 bits (2116), Expect = 0.0
 Identities = 406/490 (82%), Positives = 450/490 (91%)

>gb|AAN70628.1|AE016706_3 (AE016793) betaine aldehyde dehydrogenase [Pseudomonas putida
           KT2440]
          Length = 490

 Score =  810 bits (2092), Expect = 0.0
 Identities = 406/490 (82%), Positives = 439/490 (89%)

>gb|AAC73415.1| (AE000138) NAD+-dependent betaine aldehyde dehydrogenase
           [Escherichia coli K12]
          Length = 490

 Score =  764 bits (1974), Expect = 0.0
 Identities = 377/490 (76%), Positives = 426/490 (86%)






 
Structural Features:
pfam00171, Aldedh, Aldehyde dehydrogenase family.
CD-Length = 464 residues, 100.0% aligned
Score =  588 bits (1517), Expect = 6e-169
Identities = 247/470 (52%), Positives = 320/470 (68%), Gaps = 7/470 (1%)

COG1012, PutA, NAD-dependent aldehyde dehydrogenases 
[Energy production and conversion]
CD-Length = 472 residues,  99.8% aligned
Score =  529 bits (1365), Expect = 2e-151
Identities = 235/481 (48%), Positives = 314/481 (65%), Gaps = 12/481 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1717




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18859




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34727




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:857_cdd.html


    		




 
URL:

















    ReferenceID:35660




  
Author/Investigator(s):
        Lamark T, Kaasen I, Eshoo MW, Falkenberg P, McDougall J, Strom AR.





  
Title:
        DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli.




  
PubMed:
        1956285


MedLine:
        




    
Source:
Mol Microbiol. 1991 May;5(5):1049-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1956285

















    ReferenceID:35662




  
Author/Investigator(s):
        Canovas D, Vargas C, Kneip S, Moron MJ, Ventosa A, Bremer E, Nieto JJ.





  
Title:
        Genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium Halomonas elongata DSM 3043, USA.




  
PubMed:
        10708384


MedLine:
        




    
Source:
Microbiology. 2000 Feb;146 ( Pt 2):455-63.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10708384









           
Homologs By Global Alignment 

           

       



     
Gene ID:857
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1541
                           PA5373_tr
                           translation of PA5373
                           490
                           99.38
                           99.38
                           0
                           2481.0
     

     
     
55553
                           gb|AAG08758.1|AE004950_1
                           (AE004950) betaine aldehyde dehydrogenase [Pseudomonas aeruginosa PAO1]
                           490
                           99.38
                           99.38
                           0
                           2481.0
     

     
     
55555
                           gb|AAN70628.1|AE016706_3
                           (AE016793) betaine aldehyde dehydrogenase [Pseudomonas putida KT2440]
                           490
                           82.85
                           89.59
                           0
                           2092.0
     

     
     
55554
                           gb|AAO53985.1|
                           (AE016857) betaine aldehyde dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           490
                           82.85
                           91.83
                           0
                           2116.0
     

     
     
55558
                           emb|CAA37092.1|
                           (X52905) betB gene product (AA 1-490) [Escherichia coli]
                           490
                           76.93
                           86.93
                           0
                           1974.0
     

     






      

          
GC ORFID: 43859How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5373Glimmer Score: 

          
Start: 5587265Stop: 5585793

          
Length: 1473


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5373 translation of PA5373Homolog Bit Score 960.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6625 (ORF: BLASTX 820.0), psyr_15may02_Scaffold1_revised_gene155 (ORF: BLASTX 381.0), t_PA5312 (ORF: BLASTX 375.0), psyr_15may02_Scaffold2_revised_gene3152 (ORF: BLASTX 373.0), t_PA0219 (ORF: BLASTX 359.0), t_PA4073 (ORF: BLASTX 353.0), t_PA3504 (ORF: BLASTX 351.0), t_PA5373 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCCGATTCGAAGAACAGAAGCTCTACATTGGCGGTCGCTACGTGGAGGCCAGCAGTGGCGCCACCTTC 
GAGACCATCAACCCGGCCAACGGCGAAGTGCTCGCCAAGGTCCAGCGGGCTTCCAGGGAAGACGTCGAGCGG 
GCCGTGCAGAGCGCCGTCGAGGGGCAGAAGGTGTGGGCGGCGATGACCGCCATGCAGCGCTCGCGCATCCTC 
CGTCGCGCCGTCGACATCCTCCGCGAGCGCAACGACGAACTGGCCGCCCTGGAAACCCTGGATACCGGCAAG 
CCGCTGGCGGAAACCCGCTCGGTGGACATCGTCACCGGCGCCGACGTGCTCGAGTACTACGCCGGCCTGGTC 
CCGGCCATCGAAGGCGAGCAGATTCCCCTGCGCGAAACCAGTTTCGTCTACACCCGCCGCGAGCCGCTGGGC 
GTGGTCGCCGGCATTGGCGCCTGGAACTACCCGGTGCAGATCGCCCTGTGGAAATCCGCCCCGGCCCTCGCT 
GCCGGCAACGCGATGATCTTCAAGCCCAGCGAAGTCACCCCGCTGACCACCCTCAAGCTGGCCGAGATCTAC 
ACCGAGGCCGGCGTGCCGGACGGCGTGTTCAACGTGCTCACCGGCAGCGGACGCGAAGTCGGCCAGTGGCTG 
ACCGAGCACCCGCTGATCGAGAAGATCTCCTTCACCGGCGGCACCTCCACCGGCAAGAAAGTCATGGCCAGC 
GCTTCCAGCTCGTCGCTGAAGGAAGTGACCATGGAGCTGGGCGGCAAGTCCCCGCTGATCATCTTCCCCGAC 
GCAGACCTCGACCGCGCCGCCGACATCGCCGTCATGGCCAACTTCTTCAGCTCCGGCCAGGTCTGCACCAAT 
GGCACCCGGGTGTTCATCCACCGTTCCCAGCAGGCCCGCTTCGAAGCCAAGGTACTGGAGCGCGTGCAGCGC 
ATCCGTCTCGGCGACCCGCAGGACGAGAACACCAACTTCGGCCCGCTGGTCAGCTTCCCGCACATGGAAAGC 
GTGCTCGGCTACATCGAGTCCGGCAAGGCGCAGAAGGCTCGCCTGCTGTGCGGCGGCGAGCGTGTGACCGAT 
GGAGCGTTCGGCAATGGCGCCTACGTCGCGCCGACCGTGTTCACCGATTGCAGCGACGACATGACCATCGTC 
CGCGAGGAAATCTTCGGCCCGGTGATGAGCATCCTGGTCTATGACGACGAAGACGAGGCGATCCGCCGCGCC 
AACGATACCGAGTACGGCCTCGCCGCCGGCGTCGTGACCCAGGACCTGGCCCGCGCCCACCGCGCCATCCAT 
CGCCTGGAAGCCGGCATCTGCTGGATCAATACCTGGGGCGAGTCGCCGGCGGAAATGCCGGTCGGCGGTTAC 
AAGCAATCCGGTGTCGGTCGCGAGAACGGTCTCACCACCCTGGCTCATTACACTCGCATCAAGTCCGTGCAA 
GTGGAGCTGGGCGACTACGCCTCGGTGTTCTGA