ViewGene
Locus Name:
GeneID:
Location:

GeneID:856
TrivialName: PA14_13770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_13770
PAO1 Ortholog Locus:PA3876
Sequence Length:1407
Protein Length:468
Start: 1182125
Stop: 1183531
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07263.1|AE004805_1 (AE004805) nitrite extrusion protein 2 [Pseudomonas aeruginosa PAO1]
Identities = 466/468 (99%), Positives = 467/468 (99%)
Sequence:
ATGGGCATGATCGCCAATACCCAACTGAAAGCCGCACGGGGACCGCTGCTGCTCGACTGGCGCCCCGAGGAC
CCCGAGTTCTGGGCGCGCAACGGCAAGCGCATCGCCAGCCGCAACCTGTGGATCTCGATCCCCGCGCTGCTG
CTCGCCTTCGCCGTGTGGATGATCTGGAGCACGGTGACCGTGCGCCTGAACAGCGCCGGCTTCGCCTTCAGC
AACGACCAGCTGTTCCTGCTCGCGGCGCTGCCGTCGATCTCCGGCGCCACCCTGCGGGTGTTCTATTCGTTC
ATGGTGCCGATCTTCGGCGGCCGTCGCTGGACCGCCCTGAGCACCGCCTCGATGCTGATCCCGTGCATCTGG
CTGGGCTTCGCCGTGCAGGACCCGAGCACCCCGTACTGGGTGTTCGCCCTGATCGCGCTGCTCTGCGGCTTC
GGCGGCGGCAACTTCGCCTCGAGCATGTCCAACATCAGCTTCTTCTATCCGAAGAGCCAGCAGGGCACCGCC
CTCGGCCTGAATGCCGGGCTCGGCAACCTCGGCGTCTCGGTGATGCAGTTCGCCGTGCCGCTGGCGGTGGCC
GGCGGCCTGTTCGGCGCCTTCGGCGGCGAGCCGCAGCAGCTCGGCGACGGCGGTCGCCTGTGGCTGCAGAAC
GCCGGCTGGATCTGGGTGCCGTTCATCGCCGTGGTATCGGTCGCGGCCTGGTTCGGGATGAACGACATCGCC
GACGCCAAGGCCTCGTTCCGCGACCAGGCGGTGATCTTCAAGCGCAAGCACAACTGGCTGATGTGCTGGCTG
TACGTGGCCACCTTCGGCTCGTTCATCGGCTTCTCGGCCGGCTTCGCGATGCTCAGCAAGACCCAGTTCCCC
GACATCAACCCGCTGCAATACGCCTTCCTCGGTCCGCTGGTGGGCGCCCTCAGCCGGCCCCTGGGCGGCTGG
CTGGCGGACAAGTTCGGCGGCGCGCGGATCACCCTGTGGAACTACGCACTGATGATCGGCGGGGTGTTCACG
GTGATCGCCTTCCTGCCGGGCGACGGCGGCCAGGGCAACTTCTTCGGCTTCCTTGGCGCCTTCATCCTGCTG
TTCTTCTTCGCCGGCATCGGCAACGGTTCCACCTTCCGCATGATCCCGGTGATCTTCCGCAACGAGTGGGCC
CGGCGGGTGCGCGACGGCCAGGCCGACGAAGCCGGCGCGGCGCGCAACGCGAGCATGGAGTCGGCCGCGGTG
ATCGGCTTCTCCGCCGCCATCGGCGCCTTCGGCGGGTTCTTCATCCCGAAGGCGTTCGGCACCTCGCTGCAC
ATGACCGGCAGCGCCGAGGGCGCCCTCTACGTGTTCATCGCCTACTACCTGAGCTGCATCGTCGCCACCTGG
TGGTGGTACGCGCGCAAGGGTGCGGAGAGTCCCTGTTGA
Translation:
MGMIANTQLKAARGPLLLDWRPEDPEFWARNGKRIASRNLWISIPALLLAFAVWMIWSTVTVRLNSAGFAFS
NDQLFLLAALPSISGATLRVFYSFMVPIFGGRRWTALSTASMLIPCIWLGFAVQDPSTPYWVFALIALLCGF
GGGNFASSMSNISFFYPKSQQGTALGLNAGLGNLGVSVMQFAVPLAVAGGLFGAFGGEPQQLGDGGRLWLQN
AGWIWVPFIAVVSVAAWFGMNDIADAKASFRDQAVIFKRKHNWLMCWLYVATFGSFIGFSAGFAMLSKTQFP
DINPLQYAFLGPLVGALSRPLGGWLADKFGGARITLWNYALMIGGVFTVIAFLPGDGGQGNFFGFLGAFILL
FFFAGIGNGSTFRMIPVIFRNEWARRVRDGQADEAGAARNASMESAAVIGFSAAIGAFGGFFIPKAFGTSLH
MTGSAEGALYVFIAYYLSCIVATWWWYARKGAESPC*
AnnotationID:865GeneID:856
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 09:09:45
Gene Name:narK2
Confidence Code:2
GeneProduct:nitrite extrusion protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2223
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAG34372.1|AF197465_4 (AF197465) putative nitrate/nitrite transporter; NarK [Pseudomonas
           fluorescens]
          Length = 465

 Score =  697 bits (1799), Expect = 0.0
 Identities = 329/457 (71%), Positives = 383/457 (83%), Gaps = 1/457 (0%)

>emb|CAD18124.1| (AL646082) PROBABLE NITRITE/NITRATE TRANSPORTER TRANSMEMBRANE
           PROTEIN [Ralstonia solanacearum]
          Length = 461

 Score =  601 bits (1550), Expect = e-171
 Identities = 286/458 (62%), Positives = 355/458 (77%), Gaps = 6/458 (1%)

>gb|AAN33500.1|AE014530_9 (AE014530) nitrite extrusion protein [Brucella suis 1330]
          Length = 895

 Score =  580 bits (1495), Expect = e-165
 Identities = 276/464 (59%), Positives = 346/464 (74%), Gaps = 6/464 (1%)






 
Structural Features:
COG2223, NarK, Nitrate/nitrite transporter 
[Inorganic ion transport and metabolism]
CD-Length = 417 residues,  96.9% aligned
Score =  360 bits (925), Expect = 5e-100
Identities = 192/445 (43%), Positives = 243/445 (54%), Gaps = 48/445 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1715




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18857




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31688




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:856_cdd.html


    		




 
URL:

















    ReferenceID:34338




  
Author/Investigator(s):
        Ramirez S, Moreno R, Zafra O, Castan P, Valles C, Berenguer J.





  
Title:
        Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8.




  
PubMed:
        10735860


MedLine:
        




    
Source:
J Bacteriol. 2000 Apr;182(8):2179-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10735860









           
Homologs By Global Alignment 

           

       



     
Gene ID:856
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1540
                           PA3876_tr
                           translation of PA3876
                           468
                           99.57
                           99.78
                           0
                           2481.0
     

     
     
55509
                           gb|AAG07263.1|AE004805_1
                           (AE004805) nitrite extrusion protein 2 [Pseudomonas aeruginosa PAO1]
                           468
                           99.57
                           99.78
                           0
                           2481.0
     

     
     
55510
                           emb|CAA75539.1|
                           (Y15252) nitrate extrusion protein [Pseudomonas aeruginosa]
                           468
                           99.14
                           99.35
                           2
                           2470.0
     

     
     
55511
                           gb|AAG34372.1|AF197465_4
                           (AF197465) putative nitrate/nitrite transporter; NarK [Pseudomonas fluorescens]
                           470
                           70.85
                           82.76
                           7
                           1799.0
     

     
     
55512
                           gb|AAP86018.1|
                           (AY305378) putative nitrite/nitrate transporter [Ralstonia eutropha]
                           476
                           63.23
                           76.26
                           20
                           1610.0
     

     






      

          
GC ORFID: 43858How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3876Glimmer Score: 

          
Start: 3771813Stop: 3770407

          
Length: 1407


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3876 translation of PA3876Homolog Bit Score 960.0

          
Other Homologs:  t_PA3876 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCATGATCGCCAATACCCAACTGAAAGCCGCACGGGGACCGCTGCTGCTCGACTGGCGCCCCGAGGAC 
CCCGAGTTCTGGGCGCGCAACGGCAAGCGCATCGCCAGCCGCAACCTGTGGATCTCGATCCCCGCGCTGCTG 
CTCGCCTTCGCCGTGTGGATGATCTGGAGCACGGTGACCGTGCGCCTGAACAGCGCCGGCTTCGCCTTCAGC 
AACGACCAGCTGTTCCTGCTCGCGGCGCTGCCGTCGATCTCCGGCGCCACCCTGCGGGTGTTCTATTCGTTC 
ATGGTGCCGATCTTCGGCGGCCGTCGCTGGACCGCCCTGAGCACCGCCTCGATGCTGATCCCGTGCATCTGG 
CTGGGCTTCGCCGTGCAGGACCCGAGCACCCCGTACTGGGTGTTCGCCCTGATCGCGCTGCTCTGCGGCTTC 
GGCGGCGGCAACTTCGCCTCGAGCATGTCCAACATCAGCTTCTTCTATCCGAAGAGCCAGCAGGGCACCGCC 
CTCGGCCTGAATGCCGGGCTCGGCAACCTCGGCGTCTCGGTGATGCAGTTCGCCGTGCCGCTGGCGGTGGCC 
GGCGGCCTGTTCGGCGCCTTCGGCGGCGAGCCGCAGCAGCTCGGCGACGGCGGTCGCCTGTGGCTGCAGAAC 
GCCGGCTGGATCTGGGTGCCGTTCATCGCCGTGGTATCGGTCGCGGCCTGGTTCGGGATGAACGACATCGCC 
GACGCCAAGGCCTCGTTCCGCGACCAGGCGGTGATCTTCAAGCGCAAGCACAACTGGCTGATGTGCTGGCTG 
TACGTGGCCACCTTCGGCTCGTTCATCGGCTTCTCGGCCGGCTTCGCGATGCTCAGCAAGACCCAGTTCCCC 
GACATCAACCCGCTGCAATACGCCTTCCTCGGTCCGCTGGTGGGCGCCCTCAGCCGGCCCCTGGGCGGCTGG 
CTGGCGGACAAGTTCGGCGGCGCGCGGATCACCCTGTGGAACTACGCACTGATGATCGGCGGGGTGTTCACG 
GTGATCGCCTTCCTGCCGGGCGACGGCGGCCAGGGCAACTTCTTCGGCTTCCTTGGCGCCTTCATCCTGCTG 
TTCTTCTTCGCCGGCATCGGCAACGGTTCCACCTTCCGCATGATCCCGGTGATCTTCCGCAACGAGTGGGCC 
CGGCGGGTGCGCGACGGCCAGGCCGACGAAGCCGGCGCGGCGCGCAACGCGAGCATGGAGTCGGCCGCGGTG 
ATCGGCTTCTCCGCCGCCATCGGCGCCTTCGGCGGGTTCTTCATCCCGAAGGCGTTCGGCACCTCGCTGCAC 
ATGACCGGCAGCGCCGAGGGCGCCCTCTACGTGTTCATCGCCTACTACCTGAGCTGCATCGTCGCCACCTGG 
TGGTGGTACGCGCGCAAGGGTGCGGAGAGTCCCTGTTGA