ViewGene
Locus Name:
GeneID:
Location:

GeneID:839
TrivialName: PA14_41000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41000
PAO1 Ortholog Locus:PA1820
Sequence Length:1503
Protein Length:500
Start: 3654737
Stop: 3656239
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05209.1|AE004608_8 (AE004608) sodium/proton antiporter NhaB [Pseudomonas aeruginosa PAO1]
Identities = 499/500 (99%), Positives = 499/500 (99%)
Sequence:
GTGAGCAGTCCCCTGAGCCAGGCTTTCGCACAGAACTTCCTCGGGCATTCGCCGCGCTGGTACAAGCTGACG
GTGCTCGCCTTCCTCTTGCTCAATCCCCTGCTGCTATGGCTGGCCGGGCCGGTGACAAGCGCCTGGGTGCTG
GTCGGCGAGTTCATCTTCACCCTGGCCATGGCGCTGAAATGCTATCCGCTGCAGCCGGGCGGCCTGCTGGTG
CTGGAGGCGCTGTTGCTCGGCCTGGCTACCCCCGAGGCGCTCTATGCGGAGTTGCAGCACAACTTCCCGGTG
CTCCTGCTGCTGATGTTCATGGTCGCCGGCATCTACTTCATGAAGGACCTGCTGCTCCTGCTGTTTTCCCGC
CTGCTGCTCGGCGTGCGCTCGAAGGCCCTGCTATCGCTCCTGTTCTGCCTGCTGGCCGCCCTCCTCTCGGCC
TTCCTCGACGCCCTCACCGTGACCGCCGTGGTGATCAGCGTGGCGGTGGCATTCTTCGCCGTGTACCACCGG
GTCGCTTCGGGGCAGCGCGCCAGCGAGGACTACGATCCCGCCACCGACCGCCAGGTCCCGGAGCTGCACCGG
GCCCATCTCGAAGAATTCCGCGCCTTCCTCCGCAGCCTGCTCATGCACGCGGCGGTCGGCACCGCACTGGGC
GGCGTCTGCACCCTGGTGGGAGAACCGCAGAACCTGCTGATCGGCCACGAGGCCGGCTGGCATTTCGTCGAG
TTCTTCCGCCAGGTCGCGCCGGTATCCATGCCGGTACTGGCGGCCGGCCTGCTGACCTGCGTGCTGCTGGAG
AAGAGCCGCCGCTTCGGCTATGGCGCCCAGTTGCCGGCGGCGGTGCGCCAGGTGCTCGCCGAGTACGCCGCC
AGCGAAAGCCGCAAACGCGGAGCGCAGCAGAAGGCCGCGCTGCTGGTGCAGGCCCTGGCGGCGCTGGTACTG
ATCGTCGGGCTGGCCCTGCATGTCGCCGAGGTCGGCCTGATCGGCCTGTTGGTGATCGTGCTGATCACTGCC
TTCACCGGCGTCACCGACGAACACCAGATCGGCCGCGCCTTCCAGGAAGCCCTGCCGTTCACTGCGCTGCTG
GTGGTGTTCTTCGCGGTGGTCGCGGTGATCCACCAGCAGCACCTGTTCACCCCGATCATCCAGGCGGTGCTG
GCGCTGCCGGCGGAACGCCAGCCGGGCATGCTGTTCATCGCCAACGGCCTGCTCTCGGCGATCAGCGACAAC
GTATTCGTCGCCACCATCTACATCACCGAGGTGAAACAGGCGCTGGATGCCGGCCACATGAGCCGCGAGCAT
TTCGATACCCTGGCGGTGGCGATCAATACCGGCACCAACCTGCCCAGCGTAGCGACGCCCAACGGCCAGGCG
GCGTTCCTCTTCCTGCTGACCTCCTCCATCGCCCCGCTGGTGCGCCTGTCGTACGGCCGCATGGTCTGGATG
GCGCTACCCTACACCCTGGTGATGGGCGGCCTGGGCTGGTGGGCGGTCAGTCATTGGCTTTGA
Translation:
MSSPLSQAFAQNFLGHSPRWYKLTVLAFLLLNPLLLWLAGPVTSAWVLVGEFIFTLAMALKCYPLQPGGLLV
LEALLLGLATPEALYAELQHNFPVLLLLMFMVAGIYFMKDLLLLLFSRLLLGVRSKALLSLLFCLLAALLSA
FLDALTVTAVVISVAVAFFAVYHRVASGQRASEDYDPATDRQVPELHRAHLEEFRAFLRSLLMHAAVGTALG
GVCTLVGEPQNLLIGHEAGWHFVEFFRQVAPVSMPVLAAGLLTCVLLEKSRRFGYGAQLPAAVRQVLAEYAA
SESRKRGAQQKAALLVQALAALVLIVGLALHVAEVGLIGLLVIVLITAFTGVTDEHQIGRAFQEALPFTALL
VVFFAVVAVIHQQHLFTPIIQAVLALPAERQPGMLFIANGLLSAISDNVFVATIYITEVKQALDAGHMSREH
FDTLAVAINTGTNLPSVATPNGQAAFLFLLTSSIAPLVRLSYGRMVWMALPYTLVMGGLGWWAVSHWL*
AnnotationID:848GeneID:839
AnnotatorUID: diggins
Modification Date/Time: 2005-03-01 19:07:44
Gene Name:nhaB
Confidence Code:1
GeneProduct:sodium/proton antiporter NhaB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3067,pfam06450
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69623.1|AE016596_1 (AE016789) sodium-proton antiporter NhaB [Pseudomonas putida
           KT2440]
          Length = 500

 Score =  756 bits (1951), Expect = 0.0
 Identities = 381/500 (76%), Positives = 432/500 (86%)

>gb|AAN55902.1|AE015727_2 (AE015727) Na+/H+ antiporter [Shewanella oneidensis MR-1]
          Length = 533

 Score =  604 bits (1557), Expect = e-172
 Identities = 306/500 (61%), Positives = 385/500 (77%), Gaps = 4/500 (0%)

>gb|AAO10612.1|AE016804_122 (AE016804) Na+/H+-exchanging protein [Vibrio vulnificus CMCP6]
          Length = 529

 Score =  603 bits (1554), Expect = e-171
 Identities = 307/497 (61%), Positives = 383/497 (77%), Gaps = 1/497 (0%)






 
Structural Features:
COG3067, NhaB, Na+/H+ antiporter 
[Inorganic ion transport and metabolism]
CD-Length = 516 residues,  96.9% aligned
Score =  752 bits (1943), Expect = 0.0
Identities = 328/500 (65%), Positives = 391/500 (78%)

pfam06450, NhaB, Bacterial Na+/H+ antiporter B (NhaB).
CD-Length = 515 residues,  97.1% aligned
Score =  622 bits (1606), Expect = 2e-179
Identities = 310/500 (62%), Positives = 386/500 (77%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14235




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18823




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37103




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:839_cdd.html


    		




 
URL:

















    ReferenceID:37795




  
Author/Investigator(s):
        Kuroda T, Fujita N, Utsugi J, Kuroda M, Mizushima T, Tsuchiya T.





  
Title:
        A major Li(+) extrusion system NhaB of Pseudomonas aeruginosa : comparison with the major Na(+) extrusion system NhaP.




  
PubMed:
        15107534


MedLine:
        




    
Source:
Microbiol Immunol. 2004;48(4):243-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15107534

















    ReferenceID:37796




  
Author/Investigator(s):
        Pinner E, Padan E, Schuldiner S.





  
Title:
        Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli.




  
PubMed:
        1317851


MedLine:
        




    
Source:
J Biol Chem. 1992 Jun 5;267(16):11064-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1317851









           
Homologs By Global Alignment 

           

       



     
Gene ID:839
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1511
                           PA1820_tr
                           translation of PA1820
                           500
                           99.80
                           99.80
                           0
                           2496.0
     

     
     
54726
                           gb|AAG05209.1|AE004608_8
                           (AE004608) sodium/proton antiporter NhaB [Pseudomonas aeruginosa PAO1]
                           500
                           99.60
                           99.80
                           0
                           2493.0
     

     
     
54727
                           dbj|BAB07876.2|
                           (AB037930) Na/H antiporter [Pseudomonas aeruginosa]
                           500
                           99.40
                           99.60
                           0
                           2488.0
     

     
     
54728
                           gb|AAN69623.1|AE016596_1
                           (AE016789) sodium-proton antiporter NhaB [Pseudomonas putida KT2440]
                           500
                           76.00
                           86.40
                           0
                           1947.0
     

     
     
54740
                           emb|CAD05489.1|
                           (AL627272) regulator of intracellular pH; Na+/H+ antiporter [Salmonella enterica subsp. enterica serovar Typhi]
                           517
                           59.18
                           72.92
                           20
                           1524.0
     

     






      

          
GC ORFID: 43841How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1820Glimmer Score: 

          
Start: 1299173Stop: 1297671

          
Length: 1503


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1820 translation of PA1820Homolog Bit Score 966.0

          
Other Homologs:  t_PA1820 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGCAGTCCCCTGAGCCAGGCTTTCGCACAGAACTTCCTCGGGCATTCGCCGCGCTGGTACAAGCTGACG 
GTGCTCGCCTTCCTCTTGCTCAATCCCCTGCTGCTATGGCTGGCCGGGCCGGTGACAAGCGCCTGGGTGCTG 
GTCGGCGAGTTCATCTTCACCCTGGCCATGGCGCTGAAATGCTATCCGCTGCAGCCGGGCGGCCTGCTGGTG 
CTGGAGGCGCTGTTGCTCGGCCTGGCTACCCCCGAGGCGCTCTATGCGGAGTTGCAGCACAACTTCCCGGTG 
CTCCTGCTGCTGATGTTCATGGTCGCCGGCATCTACTTCATGAAGGACCTGCTGCTCCTGCTGTTTTCCCGC 
CTGCTGCTCGGCGTGCGCTCGAAGGCCCTGCTATCGCTCCTGTTCTGCCTGCTGGCCGCCCTCCTCTCGGCC 
TTCCTCGACGCCCTCACCGTGACCGCCGTGGTGATCAGCGTGGCGGTGGCATTCTTCGCCGTGTACCACCGG 
GTCGCTTCGGGGCAGCGCGCCAGCGAGGACTACGATCCCGCCACCGACCGCCAGGTCCCGGAGCTGCACCGG 
GCCCATCTCGAAGAATTCCGCGCCTTCCTCCGCAGCCTGCTCATGCACGCGGCGGTCGGCACCGCACTGGGC 
GGCGTCTGCACCCTGGTGGGAGAACCGCAGAACCTGCTGATCGGCCACGAGGCCGGCTGGCATTTCGTCGAG 
TTCTTCCGCCAGGTCGCGCCGGTATCCATGCCGGTACTGGCGGCCGGCCTGCTGACCTGCGTGCTGCTGGAG 
AAGAGCCGCCGCTTCGGCTATGGCGCCCAGTTGCCGGCGGCGGTGCGCCAGGTGCTCGCCGAGTACGCCGCC 
AGCGAAAGCCGCAAACGCGGAGCGCAGCAGAAGGCCGCGCTGCTGGTGCAGGCCCTGGCGGCGCTGGTACTG 
ATCGTCGGGCTGGCCCTGCATGTCGCCGAGGTCGGCCTGATCGGCCTGTTGGTGATCGTGCTGATCACTGCC 
TTCACCGGCGTCACCGACGAACACCAGATCGGCCGCGCCTTCCAGGAAGCCCTGCCGTTCACTGCGCTGCTG 
GTGGTGTTCTTCGCGGTGGTCGCGGTGATCCACCAGCAGCACCTGTTCACCCCGATCATCCAGGCGGTGCTG 
GCGCTGCCGGCGGAACGCCAGCCGGGCATGCTGTTCATCGCCAACGGCCTGCTCTCGGCGATCAGCGACAAC 
GTATTCGTCGCCACCATCTACATCACCGAGGTGAAACAGGCGCTGGATGCCGGCCACATGAGCCGCGAGCAT 
TTCGATACCCTGGCGGTGGCGATCAATACCGGCACCAACCTGCCCAGCGTAGCGACGCCCAACGGCCAGGCG 
GCGTTCCTCTTCCTGCTGACCTCCTCCATCGCCCCGCTGGTGCGCCTGTCGTACGGCCGCATGGTCTGGATG 
GCGCTACCCTACACCCTGGTGATGGGCGGCCTGGGCTGGTGGGCGGTCAGTCATTGGCTTTGA