ViewGene
Locus Name:
GeneID:
Location:

GeneID:832
TrivialName: PA14_18380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18380
PAO1 Ortholog Locus:PA3551
Sequence Length:1446
Protein Length:481
Start: 1580102
Stop: 1578657
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06939.1|AE004776_2 (AE004776) phosphomannose isomerase / guanosine 5'-diphospho-D-mannose pyrophosphorylase [Pseudomonas aeruginosa PAO1]
Identities = 481/481 (100%), Positives = 481/481 (100%)
Sequence:
ATGATCCCAGTAATCCTTTCCGGTGGCAGCGGCTCGCGACTCTGGCCTCTTTCCCGCAAGCAGTACCCCAAG
CAGTTCCTCGCCCTCACCGGCGACGACACCCTGTTCCAGCAGACCATCAAGCGCCTGGCCTTCGACGGCATG
CAGGCGCCGCTGCTGGTGTGCAACAAGGAGCACCGCTTCATCGTCCAGGAACAGCTGGAGGCGCAGAACCTG
GCGAGCCAGGCGATCCTGCTCGAACCCTTCGGCCGCAACACGGCGCCGGCGGTGGCCATCGCCGCGATGAAA
CTGGTCGCCGAAGGCCGCGACGAACTGCTGCTGATCCTTCCCGCCGACCACGTGATCGAGGACCAGCGCGCC
TTCCAGCAGGCCCTGGCGCTGGCCACCAACGCCGCCGAAAAGGGCGAGATGGTGCTCTTCGGCATTCCCGCC
AGCCGCCCCGAGACCGGTTACGGCTACATCCGCGCGAGCGCCGATGCGCAACTGCCGGAAGGCGTCAGCCGG
GTGCAGAGCTTCGTCGAGAAACCCGACGAAGCCCGCGCCCGCGAGTTCGTCGCCGCCGGCGGCTACTACTGG
AACAGCGGCATGTTCCTGTTCCGCGCCAGCCGCTACCTGGAAGAACTGAAGAAGCACGACGCCGACATCTAC
GACACCTGCCTGCTGGCCCTGGAGCGCAGCCAGCACGACGGCGACCTGGTGAACATCGACGCCGCCACCTTC
GAATGCTGCCCGGACAACTCCATCGACTACGCGGTGATGGAGAAGACCTCGCGCGCCTGCGTGGTGCCGCTG
TCCGCCGGCTGGAACGACGTCGGCAGCTGGTCGTCGATCTGGGACGTGCACGCCAAGGACGCCAACGGCAAC
GTCACCAAGGGCGACGTGCTGGTCCACGACAGCCACAACTGCCTGGTGCACGGCAACGGCAAGCTGGTCTCG
GTGATCGGCCTGGAGGACATCGTGGTGGTGGAAACCAAGGACGCCATGATGATCGCCCACAAGGACCGGGTG
CAGGACGTCAAGCACGTGGTCAAGGACCTCGACGCCCAGGGCCGTAGCGAGACCCAGAACCACTGCGAGGTC
TACCGCCCGTGGGGCTCCTACGACTCGGTGGACATGGGCGGCCGCTTCCAGGTCAAGCACATCACCGTGAAG
CCCGGCGCGCGCCTCTCGCTGCAGATGCACCACCACCGCGCCGAGCACTGGATCGTGGTTTCCGGGACCGCC
CAGGTGACCTGCGACGACAAGACCTTCCTGCTCACCGAGAACCAGTCGACCTACATCCCGATCGCCTCCGTG
CACCGCCTGGCCAACCCCGGCAAGATCCCGCTGGAGATCATCGAGGTGCAGTCCGGCAGCTACCTCGGCGAG
GACGACATCGAGCGCCTGGAAGACGTCTACGGGCGCACCGCAGAACCGGCCCTGCAAGTGGTCGCCGGCAGC
CGCTGA
Translation:
MIPVILSGGSGSRLWPLSRKQYPKQFLALTGDDTLFQQTIKRLAFDGMQAPLLVCNKEHRFIVQEQLEAQNL
ASQAILLEPFGRNTAPAVAIAAMKLVAEGRDELLLILPADHVIEDQRAFQQALALATNAAEKGEMVLFGIPA
SRPETGYGYIRASADAQLPEGVSRVQSFVEKPDEARAREFVAAGGYYWNSGMFLFRASRYLEELKKHDADIY
DTCLLALERSQHDGDLVNIDAATFECCPDNSIDYAVMEKTSRACVVPLSAGWNDVGSWSSIWDVHAKDANGN
VTKGDVLVHDSHNCLVHGNGKLVSVIGLEDIVVVETKDAMMIAHKDRVQDVKHVVKDLDAQGRSETQNHCEV
YRPWGSYDSVDMGGRFQVKHITVKPGARLSLQMHHHRAEHWIVVSGTAQVTCDDKTFLLTENQSTYIPIASV
HRLANPGKIPLEIIEVQSGSYLGEDDIERLEDVYGRTAEPALQVVAGSR*
AnnotationID:841GeneID:832
AnnotatorUID: danlee
Modification Date/Time: 2006-03-11 16:04:13
Gene Name:algA
Confidence Code:1
GeneProduct:mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
Cell Localization:()
Synonyms:xanB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:alginate biosynthesis protein AlgA
Functional Category Confidence Code:5
COGs:pfam01050,pfam00483,COG0836
Secondary Functional Category(ies):Adaptation, protection; Cell wall / LPS / capsule
EC Number:5.3.1.8, 2.7.7.22
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66901.1|AE016318_8 (AE016778) mannose-1-phosphate
           guanylyltransferase/mannose-6-phosphate isomerase
           [Pseudomonas putida KT2440]
          Length = 485

 Score =  813 bits (2099), Expect = 0.0
 Identities = 390/472 (82%), Positives = 432/472 (91%)

>gb|AAM38423.1| (AE012008) phosphomannose isomerase; GDP-mannose pyrophosphorylase
           [Xanthomonas axonopodis pv. citri str. 306]
          Length = 467

 Score =  573 bits (1477), Expect = e-163
 Identities = 284/468 (60%), Positives = 350/468 (74%), Gaps = 5/468 (1%)

>dbj|BAB36277.1| (AP002559) mannose-1-phosphate guanyltransferase [Escherichia coli
           O157:H7]
          Length = 478

 Score =  522 bits (1344), Expect = e-147
 Identities = 263/472 (55%), Positives = 337/472 (71%), Gaps = 4/472 (0%)






 
Structural Features:
pfam01050, MannoseP_isomer, Mannose-6-phosphate isomerase.
CD-Length = 167 residues, 100.0% aligned
Score =  299 bits (768), Expect = 4e-82
Identities = 114/167 (68%), Positives = 139/167 (83%)

pfam00483, NTP_transferase, Nucleotidyl transferase.
CD-Length = 246 residues, 100.0% aligned
Score =  235 bits (602), Expect = 7e-63
Identities = 99/288 (34%), Positives = 138/288 (47%), Gaps = 44/288 (15%)

COG0836, {ManC}, Mannose-1-phosphate guanylyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 333 residues,  99.7% aligned
Score =  477 bits (1230), Expect = 1e-135
Identities = 190/334 (56%), Positives = 243/334 (72%), Gaps = 4/334 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1667




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18809




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32018




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:832_cdd.html


    		




 
URL:

















    ReferenceID:34143




  
Author/Investigator(s):
        Malhotra S, Silo-Suh LA, Mathee K, Ohman DE.





  
Title:
        Proteome analysis of the effect of mucoid conversion on global protein expression in Pseudomonas aeruginosa strain PAO1 shows induction of the disulfide bond isomerase, dsbA.




  
PubMed:
        11092861


MedLine:
        




    
Source:
J Bacteriol. 2000 Dec;182(24):6999-7006.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11092861

















    ReferenceID:34144




  
Author/Investigator(s):
        May TB, Shinabarger D, Boyd A, Chakrabarty AM.





  
Title:
        Identification of amino acid residues involved in the activity of phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa.




  
PubMed:
        8106458


MedLine:
        




    
Source:
J Biol Chem. 1994 Feb 18;269(7):4872-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8106458









           
Homologs By Global Alignment 

           

       



     
Gene ID:832
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1502
                           PA3551_tr
                           translation of PA3551
                           481
                           99.99
                           99.99
                           0
                           2502.0
     

     
     
54465
                           gb|AAG06939.1|AE004776_2
                           (AE004776) phosphomannose isomerase / guanosine 5'-diphospho-D-mannose pyrophosphorylase [Pseudomonas aeruginosa PAO1]
                           481
                           99.99
                           99.99
                           0
                           2502.0
     

     
     
54466
                           gb|AAA25972.1|
                           (M14037) phosphomannose isomerase [Pseudomonas aeruginosa]
                           481
                           98.75
                           99.79
                           0
                           2482.0
     

     
     
54467
                           gb|AAN66901.1|AE016318_8
                           (AE016778) mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase [Pseudomonas putida KT2440]
                           487
                           80.28
                           89.73
                           8
                           2095.0
     

     
     
54468
                           gb|AAO54757.1|
                           (AE016860) alginate biosynthesis protein AlgA [Pseudomonas syringae pv. tomato str. DC3000]
                           487
                           79.26
                           88.91
                           10
                           2053.0
     

     






      

          
GC ORFID: 43834How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3551Glimmer Score: 

          
Start: 3373833Stop: 3375278

          
Length: 1446


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3551 translation of PA3551Homolog Bit Score 968.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5002 (ORF: BLASTX 798.0), psyr_15may02_Scaffold3_revised_gene4287 (ORF: BLASTX 524.0), t_PA5452 (ORF: BLASTX 416.0), t_PA2232 (ORF: BLASTX 412.0), psyr_15may02_Scaffold11_revised_gene1912 (ORF: BLASTX 402.0), t_PA3551 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCCAGTAATCCTTTCCGGTGGCAGCGGCTCGCGACTCTGGCCTCTTTCCCGCAAGCAGTACCCCAAG 
CAGTTCCTCGCCCTCACCGGCGACGACACCCTGTTCCAGCAGACCATCAAGCGCCTGGCCTTCGACGGCATG 
CAGGCGCCGCTGCTGGTGTGCAACAAGGAGCACCGCTTCATCGTCCAGGAACAGCTGGAGGCGCAGAACCTG 
GCGAGCCAGGCGATCCTGCTCGAACCCTTCGGCCGCAACACGGCGCCGGCGGTGGCCATCGCCGCGATGAAA 
CTGGTCGCCGAAGGCCGCGACGAACTGCTGCTGATCCTTCCCGCCGACCACGTGATCGAGGACCAGCGCGCC 
TTCCAGCAGGCCCTGGCGCTGGCCACCAACGCCGCCGAAAAGGGCGAGATGGTGCTCTTCGGCATTCCCGCC 
AGCCGCCCCGAGACCGGTTACGGCTACATCCGCGCGAGCGCCGATGCGCAACTGCCGGAAGGCGTCAGCCGG 
GTGCAGAGCTTCGTCGAGAAACCCGACGAAGCCCGCGCCCGCGAGTTCGTCGCCGCCGGCGGCTACTACTGG 
AACAGCGGCATGTTCCTGTTCCGCGCCAGCCGCTACCTGGAAGAACTGAAGAAGCACGACGCCGACATCTAC 
GACACCTGCCTGCTGGCCCTGGAGCGCAGCCAGCACGACGGCGACCTGGTGAACATCGACGCCGCCACCTTC 
GAATGCTGCCCGGACAACTCCATCGACTACGCGGTGATGGAGAAGACCTCGCGCGCCTGCGTGGTGCCGCTG 
TCCGCCGGCTGGAACGACGTCGGCAGCTGGTCGTCGATCTGGGACGTGCACGCCAAGGACGCCAACGGCAAC 
GTCACCAAGGGCGACGTGCTGGTCCACGACAGCCACAACTGCCTGGTGCACGGCAACGGCAAGCTGGTCTCG 
GTGATCGGCCTGGAGGACATCGTGGTGGTGGAAACCAAGGACGCCATGATGATCGCCCACAAGGACCGGGTG 
CAGGACGTCAAGCACGTGGTCAAGGACCTCGACGCCCAGGGCCGTAGCGAGACCCAGAACCACTGCGAGGTC 
TACCGCCCGTGGGGCTCCTACGACTCGGTGGACATGGGCGGCCGCTTCCAGGTCAAGCACATCACCGTGAAG 
CCCGGCGCGCGCCTCTCGCTGCAGATGCACCACCACCGCGCCGAGCACTGGATCGTGGTTTCCGGGACCGCC 
CAGGTGACCTGCGACGACAAGACCTTCCTGCTCACCGAGAACCAGTCGACCTACATCCCGATCGCCTCCGTG 
CACCGCCTGGCCAACCCCGGCAAGATCCCGCTGGAGATCATCGAGGTGCAGTCCGGCAGCTACCTCGGCGAG 
GACGACATCGAGCGCCTGGAAGACGTCTACGGGCGCACCGCAGAACCGGCCCTGCAAGTGGTCGCCGGCAGC 
CGCTGA