ViewGene
Locus Name:
GeneID:
Location:

GeneID:831
TrivialName: PA14_31920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31920
PAO1 Ortholog Locus:PA2525
Sequence Length:1497
Protein Length:498
Start: 2779567
Stop: 2781063
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05913.1|AE004680_1 (AE004680) probable outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 498/498 (100%), Positives = 498/498 (100%)
Sequence:
ATGAAACACACCCCCTCGTTGCTCGCCCTGGCCCTGGTCGCCGCCCTCGGCGGCTGCGCCATCGGCCCCGAC
TACCAGCGACCGGACCTGGCGGTGCCCGCCGAATTCAAGGAAGCCGAAGGCTGGCGCCGCGCCGAGCCGCGC
GACGTGTTCCAGCGCGGCGCCTGGTGGGAGCTGTACGGCGACCAGACCCTGAACGACCTGCAGATGCACCTG
GAACGTTCCAACCAGACCCTGGCCCAGTCGGTGGCGCAGTTCCGCCAGGCCGAGGCGCTGGTGCGCGGCGCG
CGGGCGGCGTTCTTCCCGTCGATCACCGGCAACGTGGGCAAGACCCGCAGCGGCCAGGGCGGCGGCGATAGC
ACCGTGTTGCTGCCGGGAGGCTCGACGGTGAGCAGCGGCGGCTCTGGCGCGATCAGTACCAGCTACTCGACC
AACCTCAGTGTCAGCTGGGAGGTCGACCTCTGGGGCAAGCTGCGCCGGCAACTGGAGGCCAACCAGGCGAGC
CTGCATGCCAGCGCCGCCGACCTCGCCGCGGTGCGCCTCAGCCAGCAGTCGCAACTGGCGCAGAACTACCTG
CAACTGCGGGTGATGGACGAACAGATCCGCCTGCTCAACGACACGGTGACGGCCTACGAGCGTTCGCTGAAG
GTGGCCGAGAACAAATACCGCGCCGGCATCGTCACCAGGGCCGACGTGGCCCAGGCCCGCACCCAGCTGAAA
AGCACCCAGGCCCAGGCCATCGACCTGAAGTACCAGCGCGCCCAGCTGGAGCACGCCATCGCCGTGCTGGTC
GGCCTGCCGCCGGCGCAATTCAACCTGCCGCCGGTGGCGAGCGTGCCGAAGCTGCCGGACCTGCCGGCAGTG
GTGCCGTCGCAATTGCTCGAACGACGGCCGGACATCGCCTCGGCGGAACGCAAGGTGATTTCCGCCAATGCC
CAGATCGGCGTGGCCAAGGCCGCCTATTTCCCCGACCTCACCCTGAGCGCCGCCGGCGGCTACCGCAGCGGC
AGCCTGAGCAACTGGATCAGCACGCCGAACCGCTTCTGGTCGATCGGCCCGCAGTTCGCCATGACCCTGTTC
GACGGCGGCCTGATCGGCTCCCAGGTGGACCAGGCCGAGGCGACCTACGACCAGACCGTGGCGACCTACCGG
CAGACCGTGCTCGACGGTTTCCGCGAGGTGGAGGACTACCTGGTGCAATTGAGCGTCCTCGACGAGGAGAGC
GGGGTGCAGCGCGAAGCCCTGGAGTCGGCCCGCGAGGCACTGCGCCTGGCCGAGAACCAGTACAAGGCCGGC
ACCGTCGACTACACCGACGTGGTCACCAACCAGGCCACCGCGCTGAGCAACGAACGCACCGTGCTGACCCTG
CTCGGCAGCCGCCTGACCGCCAGCGTCCAGTTGATCGCGGCAATGGGCGGCGGCTGGGACAGCGCCGACATC
GAGCGGACCGACGAGCGGCTCGGCCGGGTCGAGGAGGGCCTGCCGCCTTCGCCCTGA
Translation:
MKHTPSLLALALVAALGGCAIGPDYQRPDLAVPAEFKEAEGWRRAEPRDVFQRGAWWELYGDQTLNDLQMHL
ERSNQTLAQSVAQFRQAEALVRGARAAFFPSITGNVGKTRSGQGGGDSTVLLPGGSTVSSGGSGAISTSYST
NLSVSWEVDLWGKLRRQLEANQASLHASAADLAAVRLSQQSQLAQNYLQLRVMDEQIRLLNDTVTAYERSLK
VAENKYRAGIVTRADVAQARTQLKSTQAQAIDLKYQRAQLEHAIAVLVGLPPAQFNLPPVASVPKLPDLPAV
VPSQLLERRPDIASAERKVISANAQIGVAKAAYFPDLTLSAAGGYRSGSLSNWISTPNRFWSIGPQFAMTLF
DGGLIGSQVDQAEATYDQTVATYRQTVLDGFREVEDYLVQLSVLDEESGVQREALESAREALRLAENQYKAG
TVDYTDVVTNQATALSNERTVLTLLGSRLTASVQLIAAMGGGWDSADIERTDERLGRVEEGLPPSP*
AnnotationID:840GeneID:831
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 18:06:26
Gene Name:
Confidence Code:3
GeneProduct:putative outer membrane protein
Cell Localization:()
Synonyms:opmB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1538,pfam02321
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69183.1|AE016552_5 (AE016787) RND efflux transporter, outer membrane protein
           [Pseudomonas putida KT2440]
          Length = 496

 Score =  560 bits (1444), Expect = e-159
 Identities = 290/466 (62%), Positives = 352/466 (75%), Gaps = 8/466 (1%)

>emb|CAE34792.1| (BX640450) putative outer membrane efflux protein [Bordetella
           bronchiseptica]
          Length = 500

 Score =  538 bits (1387), Expect = e-152
 Identities = 276/479 (57%), Positives = 349/479 (72%), Gaps = 14/479 (2%)

>emb|CAE39239.1| (BX640435) putative outer membrane efflux protein [Bordetella
           parapertussis]
          Length = 500

 Score =  538 bits (1387), Expect = e-152
 Identities = 276/479 (57%), Positives = 349/479 (72%), Gaps = 14/479 (2%)






 
Structural Features:
COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / Intracellular trafficking and secretion]
CD-Length = 457 residues, 100.0% aligned
Score =  269 bits (689), Expect = 5e-73
Identities = 174/476 (36%), Positives = 245/476 (51%), Gaps = 21/476 (4%)

pfam02321, OEP, Outer membrane efflux protein. 
CD-Length = 188 residues, 100.0% aligned
Score =  117 bits (294), Expect = 3e-27
Identities = 61/188 (32%), Positives = 88/188 (46%), Gaps = 1/188 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1665




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18807




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33080




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:831_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:831
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1501
                           PA2525_tr
                           translation of PA2525
                           498
                           99.99
                           99.99
                           0
                           2503.0
     

     
     
54455
                           gb|AAG05913.1|AE004680_1
                           (AE004680) probable outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
                           498
                           99.99
                           99.99
                           0
                           2503.0
     

     
     
54456
                           dbj|BAC24096.1|
                           (AB085581) outer membrane efflux protein [Pseudomonas aeruginosa]
                           498
                           99.99
                           99.99
                           0
                           2503.0
     

     
     
54457
                           gb|AAO56256.1|
                           (AE016865) outer membrane efflux protein [Pseudomonas syringae pv. tomato str. DC3000]
                           509
                           59.13
                           71.31
                           30
                           1488.0
     

     
     
54458
                           gb|AAN69183.1|AE016552_5
                           (AE016787) RND efflux transporter, outer membrane protein [Pseudomonas putida KT2440]
                           518
                           57.33
                           69.69
                           42
                           1442.0
     

     






      

          
GC ORFID: 43833How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2525Glimmer Score: 

          
Start: 2174359Stop: 2172863

          
Length: 1497


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2525 translation of PA2525Homolog Bit Score 968.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1405 (ORF: BLASTX 571.0), t_PA2525 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACACACCCCCTCGTTGCTCGCCCTGGCCCTGGTCGCCGCCCTCGGCGGCTGCGCCATCGGCCCCGAC 
TACCAGCGACCGGACCTGGCGGTGCCCGCCGAATTCAAGGAAGCCGAAGGCTGGCGCCGCGCCGAGCCGCGC 
GACGTGTTCCAGCGCGGCGCCTGGTGGGAGCTGTACGGCGACCAGACCCTGAACGACCTGCAGATGCACCTG 
GAACGTTCCAACCAGACCCTGGCCCAGTCGGTGGCGCAGTTCCGCCAGGCCGAGGCGCTGGTGCGCGGCGCG 
CGGGCGGCGTTCTTCCCGTCGATCACCGGCAACGTGGGCAAGACCCGCAGCGGCCAGGGCGGCGGCGATAGC 
ACCGTGTTGCTGCCGGGAGGCTCGACGGTGAGCAGCGGCGGCTCTGGCGCGATCAGTACCAGCTACTCGACC 
AACCTCAGTGTCAGCTGGGAGGTCGACCTCTGGGGCAAGCTGCGCCGGCAACTGGAGGCCAACCAGGCGAGC 
CTGCATGCCAGCGCCGCCGACCTCGCCGCGGTGCGCCTCAGCCAGCAGTCGCAACTGGCGCAGAACTACCTG 
CAACTGCGGGTGATGGACGAACAGATCCGCCTGCTCAACGACACGGTGACGGCCTACGAGCGTTCGCTGAAG 
GTGGCCGAGAACAAATACCGCGCCGGCATCGTCACCAGGGCCGACGTGGCCCAGGCCCGCACCCAGCTGAAA 
AGCACCCAGGCCCAGGCCATCGACCTGAAGTACCAGCGCGCCCAGCTGGAGCACGCCATCGCCGTGCTGGTC 
GGCCTGCCGCCGGCGCAATTCAACCTGCCGCCGGTGGCGAGCGTGCCGAAGCTGCCGGACCTGCCGGCAGTG 
GTGCCGTCGCAATTGCTCGAACGACGGCCGGACATCGCCTCGGCGGAACGCAAGGTGATTTCCGCCAATGCC 
CAGATCGGCGTGGCCAAGGCCGCCTATTTCCCCGACCTCACCCTGAGCGCCGCCGGCGGCTACCGCAGCGGC 
AGCCTGAGCAACTGGATCAGCACGCCGAACCGCTTCTGGTCGATCGGCCCGCAGTTCGCCATGACCCTGTTC 
GACGGCGGCCTGATCGGCTCCCAGGTGGACCAGGCCGAGGCGACCTACGACCAGACCGTGGCGACCTACCGG 
CAGACCGTGCTCGACGGTTTCCGCGAGGTGGAGGACTACCTGGTGCAATTGAGCGTCCTCGACGAGGAGAGC 
GGGGTGCAGCGCGAAGCCCTGGAGTCGGCCCGCGAGGCACTGCGCCTGGCCGAGAACCAGTACAAGGCCGGC 
ACCGTCGACTACACCGACGTGGTCACCAACCAGGCCACCGCGCTGAGCAACGAACGCACCGTGCTGACCCTG 
CTCGGCAGCCGCCTGACCGCCAGCGTCCAGTTGATCGCGGCAATGGGCGGCGGCTGGGACAGCGCCGACATC 
GAGCGGACCGACGAGCGGCTCGGCCGGGTCGAGGAGGGCCTGCCGCCTTCGCCCTGA