ViewGene
Locus Name:
GeneID:
Location:

GeneID:830
TrivialName: PA14_71220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71220
PAO1 Ortholog Locus:PA5394
Sequence Length:1473
Protein Length:490
Start: 6346302
Stop: 6344830
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08779.1|AE004952_1 (AE004952) cardiolipin synthase [Pseudomonas aeruginosa PAO1]
Identities = 490/490 (100%), Positives = 490/490 (100%)
Sequence:
ATGGCCGCCACCGGAGTGGACAAGGATCGACCTCGCGCAATGACCTCCACCACCTACCTCGGCCTGCTGCTG
GTCGGCATCCAGGTACTCGGCTTCGTCGCCGCCATCCATGCCGTGCTCACCGTGCGCACCGCACAGGGCGCG
ATCGCCTGGGCCACCTCGCTGGTATTCATGCCGTACCTGACCCTGCTGCCGTACCTGGTGTTCGGTCGCAGC
CGTTTCGACGCCTACATCGAGGCGCGGCGCCAGGCCAACCGCGAGATGCACCTGGCGGCCGCCGAGCTGGAC
TGGCGTCCCTGGGTCGAGGAGGCCCTGGCGGCCCGCCAGGTCAGCGGCTACAAGGGGCTCAAGGCGCTGGTG
CGGATGACCCGCACGCCGACCCTGGCGAACAATCGGGTGCGCCTGCTGGTCAACGGCGAGGCCAGCTTCGAG
GCGATGTTCAAGGCGATTTCCGCCGCCCGCCAGGTGATCCTGGTGCAGTTCTTCATCGTCCGCGACGACGCC
CTCGGCCAGCGCCTCCAGCAACTGCTGCTGGAACGCGCGGCCAACGGCGTCGAGGTGTTCTTCCTCTATGAC
GCCATCGGCAGCCACGCGCTGCCGCACCGCTATGTCGAGCGGCTGCGCCAGGGCGGCGTGCAGATGCATGGC
TTCAGCACCGGCAGCGGCATGCTCAACCGCTTCCAGGTGAATTTCCGCAACCACCGCAAGGTCGTGGTGGTG
GATGGCGAGTGCGGTTTCGTCGGCGGGCATAACGTCGGCGTCGAGTACCTCGGCGAGAAGCCGCCGCTGGCG
CCCTGGCGCGACACCCACATGGAACTGCGCGGGCCGGCGGTGGCGTGCCTGCAGGAAAGCTTCGCCGAGGAC
TGGTACTGGGCGACCCACAGCCTGCCGCCGCTGATCCTGCCGCCGCAGTACGACAGCGAGGGCGCGCTCTGC
CAGGTGGTCGCCAGCGGCCCGGCGGATGCCCAGGAAACCTGCTCGCTGTTCTTCGTCGAGATGATCAACGCC
GCCCACGAGCGGGTCTGGATCACCTCGCCGTATTTCGTTCCCGACGAAGCGGTGATGGCCGCCCTGCGCCTG
GCGGTGCTGCGCGGCGTCGACGTGCGCCTGCTGATCCCCTCGCGCCCGGACCACCGCACGGTGTATGCCGCG
TCCAGCCTCTACGCGCTGGAAGCGATCCGTGCCGGGGTCAAGGTGTTCCGCTACCAGCCCGGCTTCCTCCAC
CAGAAGGTGGTGCTGGTGGACCGCGACACCGCCGCCGTCGGCAGCGCCAACCTGGACAACCGTTCGTTCCGG
CTGAATTTCGAGGTGATGGTGGTGACCGTCGACGAGGGCTTCGCCGGCGAGGTGGAGGCGATGCTCGAGGCG
GACTTCGCCGAGTCGCTCGAATTCACTCCGGAAGACCGCCGCAGCGTGCGCCGCCTGCAGCAACTGGGGATG
CGCGTGGCGCGGCTGGTCTCGCCGATCCTCTGA
Translation:
MAATGVDKDRPRAMTSTTYLGLLLVGIQVLGFVAAIHAVLTVRTAQGAIAWATSLVFMPYLTLLPYLVFGRS
RFDAYIEARRQANREMHLAAAELDWRPWVEEALAARQVSGYKGLKALVRMTRTPTLANNRVRLLVNGEASFE
AMFKAISAARQVILVQFFIVRDDALGQRLQQLLLERAANGVEVFFLYDAIGSHALPHRYVERLRQGGVQMHG
FSTGSGMLNRFQVNFRNHRKVVVVDGECGFVGGHNVGVEYLGEKPPLAPWRDTHMELRGPAVACLQESFAED
WYWATHSLPPLILPPQYDSEGALCQVVASGPADAQETCSLFFVEMINAAHERVWITSPYFVPDEAVMAALRL
AVLRGVDVRLLIPSRPDHRTVYAASSLYALEAIRAGVKVFRYQPGFLHQKVVLVDRDTAAVGSANLDNRSFR
LNFEVMVVTVDEGFAGEVEAMLEADFAESLEFTPEDRRSVRRLQQLGMRVARLVSPIL*
AnnotationID:839GeneID:830
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 09:09:03
Gene Name:cls
Confidence Code:2
GeneProduct:cardiolipin synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1502
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70929.1|AE016736_12 (AE016794) cardiolipin synthase [Pseudomonas putida KT2440]
          Length = 481

 Score =  679 bits (1752), Expect = 0.0
 Identities = 327/475 (68%), Positives = 389/475 (81%)

>gb|AAO58949.1| (AE016876) cardiolipin synthetase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 479

 Score =  671 bits (1730), Expect = 0.0
 Identities = 323/472 (68%), Positives = 388/472 (82%)








 
Structural Features:
COG1502, Cls, 
Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin
synthases and related enzymes 
[Lipid metabolism]
CD-Length = 438 residues, 100.0% aligned
Score =  319 bits (818), Expect = 4e-88
Identities = 173/452 (38%), Positives = 243/452 (53%), Gaps = 33/452 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1663




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18805




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34702




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:830_cdd.html


    		




 
URL:

















    ReferenceID:35656




  
Author/Investigator(s):
        Guo D, Tropp BE.





  
Title:
        Cloning of the Bacillus firmus OF4 cls gene and characterization of its gene product.




  
PubMed:
        9443601


MedLine:
        




    
Source:
Biochim Biophys Acta. 1998 Jan 5;1389(1):34-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9443601

















    ReferenceID:35657




  
Author/Investigator(s):
        Tropp BE.





  
Title:
        Cardiolipin synthase from Escherichia coli.




  
PubMed:
        9370333


MedLine:
        




    
Source:
Biochim Biophys Acta. 1997 Sep 4;1348(1-2):192-200.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9370333









           
Homologs By Global Alignment 

           

       



     
Gene ID:830
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1500
                           PA5394_tr
                           translation of PA5394
                           490
                           99.99
                           99.99
                           0
                           2504.0
     

     
     
54445
                           gb|AAG08779.1|AE004952_1
                           (AE004952) cardiolipin synthase [Pseudomonas aeruginosa PAO1]
                           490
                           99.99
                           99.99
                           0
                           2504.0
     

     
     
54446
                           gb|AAN70929.1|AE016736_12
                           (AE016794) cardiolipin synthase [Pseudomonas putida KT2440]
                           494
                           66.39
                           78.94
                           17
                           1743.0
     

     
     
54448
                           gb|AAO58949.1|
                           (AE016876) cardiolipin synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           490
                           66.32
                           79.79
                           11
                           1724.0
     

     
     
54447
                           emb|CAA39233.1|
                           (X55704) unnamed protein product [Pseudomonas putida]
                           494
                           66.19
                           78.34
                           17
                           1726.0
     

     






      

          
GC ORFID: 43832How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5394Glimmer Score: 

          
Start: 5609991Stop: 5608519

          
Length: 1473


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5394 translation of PA5394Homolog Bit Score 969.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2927 (ORF: BLASTX 664.0), t_PA5394 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGCCACCGGAGTGGACAAGGATCGACCTCGCGCAATGACCTCCACCACCTACCTCGGCCTGCTGCTG 
GTCGGCATCCAGGTACTCGGCTTCGTCGCCGCCATCCATGCCGTGCTCACCGTGCGCACCGCACAGGGCGCG 
ATCGCCTGGGCCACCTCGCTGGTATTCATGCCGTACCTGACCCTGCTGCCGTACCTGGTGTTCGGTCGCAGC 
CGTTTCGACGCCTACATCGAGGCGCGGCGCCAGGCCAACCGCGAGATGCACCTGGCGGCCGCCGAGCTGGAC 
TGGCGTCCCTGGGTCGAGGAGGCCCTGGCGGCCCGCCAGGTCAGCGGCTACAAGGGGCTCAAGGCGCTGGTG 
CGGATGACCCGCACGCCGACCCTGGCGAACAATCGGGTGCGCCTGCTGGTCAACGGCGAGGCCAGCTTCGAG 
GCGATGTTCAAGGCGATTTCCGCCGCCCGCCAGGTGATCCTGGTGCAGTTCTTCATCGTCCGCGACGACGCC 
CTCGGCCAGCGCCTCCAGCAACTGCTGCTGGAACGCGCGGCCAACGGCGTCGAGGTGTTCTTCCTCTATGAC 
GCCATCGGCAGCCACGCGCTGCCGCACCGCTATGTCGAGCGGCTGCGCCAGGGCGGCGTGCAGATGCATGGC 
TTCAGCACCGGCAGCGGCATGCTCAACCGCTTCCAGGTGAATTTCCGCAACCACCGCAAGGTCGTGGTGGTG 
GATGGCGAGTGCGGTTTCGTCGGCGGGCATAACGTCGGCGTCGAGTACCTCGGCGAGAAGCCGCCGCTGGCG 
CCCTGGCGCGACACCCACATGGAACTGCGCGGGCCGGCGGTGGCGTGCCTGCAGGAAAGCTTCGCCGAGGAC 
TGGTACTGGGCGACCCACAGCCTGCCGCCGCTGATCCTGCCGCCGCAGTACGACAGCGAGGGCGCGCTCTGC 
CAGGTGGTCGCCAGCGGCCCGGCGGATGCCCAGGAAACCTGCTCGCTGTTCTTCGTCGAGATGATCAACGCC 
GCCCACGAGCGGGTCTGGATCACCTCGCCGTATTTCGTTCCCGACGAAGCGGTGATGGCCGCCCTGCGCCTG 
GCGGTGCTGCGCGGCGTCGACGTGCGCCTGCTGATCCCCTCGCGCCCGGACCACCGCACGGTGTATGCCGCG 
TCCAGCCTCTACGCGCTGGAAGCGATCCGTGCCGGGGTCAAGGTGTTCCGCTACCAGCCCGGCTTCCTCCAC 
CAGAAGGTGGTGCTGGTGGACCGCGACACCGCCGCCGTCGGCAGCGCCAACCTGGACAACCGTTCGTTCCGG 
CTGAATTTCGAGGTGATGGTGGTGACCGTCGACGAGGGCTTCGCCGGCGAGGTGGAGGCGATGCTCGAGGCG 
GACTTCGCCGAGTCGCTCGAATTCACTCCGGAAGACCGCCGCAGCGTGCGCCGCCTGCAGCAACTGGGGATG 
CGCGTGGCGCGGCTGGTCTCGCCGATCCTCTGA