ViewGene
Locus Name:
GeneID:
Location:

GeneID:827
TrivialName: PA14_66560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66560
PAO1 Ortholog Locus:PA5035
Sequence Length:1434
Protein Length:477
Start: 5939069
Stop: 5937636
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08420.1|AE004916_9 (AE004916) glutamate synthase small chain [Pseudomonas aeruginosa PAO1]
Identities = 477/477 (100%), Positives = 477/477 (100%)
Sequence:
ATGTCTGAACGTCTGAATTCCGACCGCCTGAACAACGACTTCCAGTTCATCGAAGTCGGGCGCAAGGACCCG
AAGAAGAAGCTGCTGCGCCAGCGCAAGCGCGAGTTCGTGGAGATCCACGACCTGTTCAAGCCGCAGCAGGCC
GCCGACCAGGCCCACCGCTGCCTGGGCTGCGGCAACCCCTACTGCGAGTGGAAGTGCCCGGTGCACAACTTC
ATCCCGAACTGGCTGAAGCTGGTTTCCGAAGGCAACATCCTGGCCGCCGCCGAACTGTCGCACCAGACCAAC
ACCCTGCCGGAAGTCTGCGGCCGGGTCTGCCCGCAGGACCGCCTCTGCGAGGGTGCCTGTACCCTCAACGAC
GGCTTTGGCGCGGTGACCATCGGCTCGGTGGAGAAATACATCACCGATACGGCCTTCGCCATGGGCTGGCGC
CCGGACCTGTCCAAGGTCAAGCCGACCGGCAGGCGCGTCGCGGTGATCGGTGCCGGCCCGGCCGGCCTCGGC
TGCGCCGACGTGCTGGTGCGCAACGGCGTGACCCCGGTGGTGTTCGACCGCAACCCGGAGATCGGCGGCCTG
CTGACCTTCGGCATTCCCGAGTTCAAGCTGGAGAAGCACGTCCTCAGCCGTCGCCGCGAGGTGTTCACCGGC
ATGGGCATCGAGTTCCGCCTGAACACCGAGATCGGCAAGGACGTGACCATGGAGCAGTTGCTCGATGAGTAC
GATGCCGTGTTCATGGGGATGGGCACCTACACCTACATGAAGGGCGGTTTCCCCGGCGAGGACCTGCCGGGC
GTACACGACGCCCTGGACTTCCTGATCGCCAACGTGAACCGCAACCTCGGCTTCGAGAAGTCGGCCGAGGAC
TTCGTCGACATGAAGGGCAAGCGCGTGGTGGTCCTGGGCGGCGGCGACACCGCGATGGACTGCAACCGCACT
TCGATCCGCCAGGGCGCCAAGTCGGTGACCTGTGCCTATCGCCGTGACGAAGAAAACATGCCGGGCTCGCGC
AAGGAAGTGAAGAACGCCAAGGAGGAGGGCGTGAAGTTCCTCTTCAACCGCCAGCCCATCGCCATCGTCGGC
GAGGACCGGGTGGAAGGCGTGAAGGTGGTCGAGACCCGTCTCGGCGAGCCGGACGCCCGCGGACGCCGCAGC
CCCGAACCTATCCCCGGTTCCGAGGAGATCATCCCGGCGGAAGCCGTGCTGATCGCCTTTGGCTTCCGGCCG
AGTCCGGCGCCGTGGTTCGAGCGCTTCGAGATTGCCACCGACAGCCAGGGCCGCGTGGTGGCTCCGGCGCAG
GCGCAGTTCAAGCACCAGACCAGCAACCCGAAGATCTTCGCCGGCGGCGACATGGTCCGTGGTTCCGACCTG
GTGGTGACGGCGATCTTCGAAGGACGTACCGCGGCCGAAGGCATCCTCGACTACCTTGGCGTCTGA
Translation:
MSERLNSDRLNNDFQFIEVGRKDPKKKLLRQRKREFVEIHDLFKPQQAADQAHRCLGCGNPYCEWKCPVHNF
IPNWLKLVSEGNILAAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWR
PDLSKVKPTGRRVAVIGAGPAGLGCADVLVRNGVTPVVFDRNPEIGGLLTFGIPEFKLEKHVLSRRREVFTG
MGIEFRLNTEIGKDVTMEQLLDEYDAVFMGMGTYTYMKGGFPGEDLPGVHDALDFLIANVNRNLGFEKSAED
FVDMKGKRVVVLGGGDTAMDCNRTSIRQGAKSVTCAYRRDEENMPGSRKEVKNAKEEGVKFLFNRQPIAIVG
EDRVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPAEAVLIAFGFRPSPAPWFERFEIATDSQGRVVAPAQ
AQFKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRTAAEGILDYLGV*
AnnotationID:836GeneID:827
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 10:10:26
Gene Name:gltD
Confidence Code:2
GeneProduct:glutamate synthase small chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0493
Secondary Functional Category(ies):
EC Number:1.4.1.13
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58548.1| (AE016874) glutamate synthase, small subunit [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 472

 Score =  900 bits (2326), Expect = 0.0
 Identities = 432/471 (91%), Positives = 460/471 (97%)

>gb|AAN70640.1|AE016708_1 (AE016793) glutamate synthase, small subunit [Pseudomonas putida
           KT2440]
          Length = 472

 Score =  896 bits (2316), Expect = 0.0
 Identities = 432/471 (91%), Positives = 456/471 (96%)

>gb|AAF95520.1| (AE004308) glutamate synthase, small subunit [Vibrio cholerae]
          Length = 471

 Score =  668 bits (1724), Expect = 0.0
 Identities = 323/468 (69%), Positives = 379/468 (80%), Gaps = 3/468 (0%)






 
Structural Features:
COG0493, GltD, NADPH-dependent glutamate synthase beta chain and related oxidoreductases 
[Amino acid transport and metabolism]
CD-Length = 457 residues,  98.2% aligned
Score =  497 bits (1282), Expect = 9e-142
Identities = 209/454 (46%), Positives = 261/454 (57%), Gaps = 15/454 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1657




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18799




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35039




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:827_cdd.html


    		




 
URL:

















    ReferenceID:35856




  
Author/Investigator(s):
        Pelanda R, Vanoni MA, Perego M, Piubelli L, Galizzi A, Curti B, Zanetti G.





  
Title:
        Glutamate synthase genes of the diazotroph Azospirillum brasilense. Cloning, sequencing, and analysis of functional domains.




  
PubMed:
        8428988


MedLine:
        




    
Source:
J Biol Chem. 1993 Feb 15;268(5):3099-106.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8428988

















    ReferenceID:35857




  
Author/Investigator(s):
        Deane SM, Rawlings DE.





  
Title:
        Cloning and sequencing of the gene for the Thiobacillus ferrooxidans ATCC33020 glutamate synthase (GOGAT) small subunit and complementation of an Escherichia coli gltD mutant.




  
PubMed:
        8921877


MedLine:
        




    
Source:
Gene. 1996 Oct 24;177(1-2):261-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8921877

















    ReferenceID:35858




  
Author/Investigator(s):
        Lu T, Wu YQ, Song HY.





  
Title:
        The Nucleotide Sequence of gltD Gene Encoding the Small Subunit of Rhodobacter sphaeroides Glutamate Synthase.




  
PubMed:
        12219208


MedLine:
        




    
Source:
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29(3):294-302. 	






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12219208









           
Homologs By Global Alignment 

           

       



     
Gene ID:827
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1496
                           PA5035_tr
                           translation of PA5035
                           477
                           99.99
                           99.99
                           0
                           2514.0
     

     
     
54319
                           gb|AAG08420.1|AE004916_9
                           (AE004916) glutamate synthase small chain [Pseudomonas aeruginosa PAO1]
                           477
                           99.99
                           99.99
                           0
                           2514.0
     

     
     
54320
                           gb|AAB39260.1|
                           (U81261) glutamate synthase small subunit [Pseudomonas aeruginosa]
                           477
                           99.99
                           99.99
                           0
                           2514.0
     

     
     
54321
                           gb|AAO58548.1|
                           (AE016874) glutamate synthase, small subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           477
                           90.56
                           96.43
                           5
                           2324.0
     

     
     
54322
                           gb|AAN70640.1|AE016708_1
                           (AE016793) glutamate synthase, small subunit [Pseudomonas putida KT2440]
                           477
                           90.56
                           95.59
                           5
                           2314.0
     

     






      

          
GC ORFID: 43829How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5035Glimmer Score: 

          
Start: 5202752Stop: 5201319

          
Length: 1434


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5035 translation of PA5035Homolog Bit Score 972.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3299 (ORF: BLASTX 906.0), t_PA5035 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTGAACGTCTGAATTCCGACCGCCTGAACAACGACTTCCAGTTCATCGAAGTCGGGCGCAAGGACCCG 
AAGAAGAAGCTGCTGCGCCAGCGCAAGCGCGAGTTCGTGGAGATCCACGACCTGTTCAAGCCGCAGCAGGCC 
GCCGACCAGGCCCACCGCTGCCTGGGCTGCGGCAACCCCTACTGCGAGTGGAAGTGCCCGGTGCACAACTTC 
ATCCCGAACTGGCTGAAGCTGGTTTCCGAAGGCAACATCCTGGCCGCCGCCGAACTGTCGCACCAGACCAAC 
ACCCTGCCGGAAGTCTGCGGCCGGGTCTGCCCGCAGGACCGCCTCTGCGAGGGTGCCTGTACCCTCAACGAC 
GGCTTTGGCGCGGTGACCATCGGCTCGGTGGAGAAATACATCACCGATACGGCCTTCGCCATGGGCTGGCGC 
CCGGACCTGTCCAAGGTCAAGCCGACCGGCAGGCGCGTCGCGGTGATCGGTGCCGGCCCGGCCGGCCTCGGC 
TGCGCCGACGTGCTGGTGCGCAACGGCGTGACCCCGGTGGTGTTCGACCGCAACCCGGAGATCGGCGGCCTG 
CTGACCTTCGGCATTCCCGAGTTCAAGCTGGAGAAGCACGTCCTCAGCCGTCGCCGCGAGGTGTTCACCGGC 
ATGGGCATCGAGTTCCGCCTGAACACCGAGATCGGCAAGGACGTGACCATGGAGCAGTTGCTCGATGAGTAC 
GATGCCGTGTTCATGGGGATGGGCACCTACACCTACATGAAGGGCGGTTTCCCCGGCGAGGACCTGCCGGGC 
GTACACGACGCCCTGGACTTCCTGATCGCCAACGTGAACCGCAACCTCGGCTTCGAGAAGTCGGCCGAGGAC 
TTCGTCGACATGAAGGGCAAGCGCGTGGTGGTCCTGGGCGGCGGCGACACCGCGATGGACTGCAACCGCACT 
TCGATCCGCCAGGGCGCCAAGTCGGTGACCTGTGCCTATCGCCGTGACGAAGAAAACATGCCGGGCTCGCGC 
AAGGAAGTGAAGAACGCCAAGGAGGAGGGCGTGAAGTTCCTCTTCAACCGCCAGCCCATCGCCATCGTCGGC 
GAGGACCGGGTGGAAGGCGTGAAGGTGGTCGAGACCCGTCTCGGCGAGCCGGACGCCCGCGGACGCCGCAGC 
CCCGAACCTATCCCCGGTTCCGAGGAGATCATCCCGGCGGAAGCCGTGCTGATCGCCTTTGGCTTCCGGCCG 
AGTCCGGCGCCGTGGTTCGAGCGCTTCGAGATTGCCACCGACAGCCAGGGCCGCGTGGTGGCTCCGGCGCAG 
GCGCAGTTCAAGCACCAGACCAGCAACCCGAAGATCTTCGCCGGCGGCGACATGGTCCGTGGTTCCGACCTG 
GTGGTGACGGCGATCTTCGAAGGACGTACCGCGGCCGAAGGCATCCTCGACTACCTTGGCGTCTGA