ViewGene
Locus Name:
GeneID:
Location:

GeneID:819
TrivialName: PA14_05770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05770
PAO1 Ortholog Locus:PA0441
Sequence Length:1440
Protein Length:479
Start: 511006
Stop: 509567
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03830.1|AE004481_6 (AE004481) dihydropyrimidinase [Pseudomonas aeruginosa PAO1]
Identities = 479/479 (100%), Positives = 479/479 (100%)
Sequence:
ATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACTCACGAAGAGAGTTATCGAGCCGACGTGCTCTGTGCC
AACGGCCTCATCCAGGCCATCGGCGAGAACCTCGAAACCCCCTCCGGCTGCGACGTCCTCGACGGCGGTGGC
CAGTACCTGATGCCGGGCGGCATCGACCCCCATACCCACATGCAGTTGCCCTTCATGGGCACGGTGGCCAGC
GAGGACTTCTTCAGCGGCACCGCGGCCGGGCTGGCCGGAGGGACCACCTCGATCATCGACTTCGTCATCCCC
AACCCGCGACAGTCGCTGCTGGAGGCCTTCCACACCTGGCGCGGCTGGGCGCAGAAATCCGCTGCGGACTAC
GGCTTCCACGTCGCCATCACCTGGTGGAGCGACGAGGTCGCCCGGGAAATGGGCGAGCTGGTGGCACAGCAC
GGGGTGAACAGCTTCAAGCACTTCATGGCCTACAAGAACGCCATCATGGCCGCCGACGATACCCTGGTGGCC
AGCTTCGAGCGCTGCCTGGAGCTGGGCGCGGTGCCGACGGTGCACGCGGAGAACGGCGAACTGGTCTTCCAC
CTGCAGCAGAAACTCCTCGCCCAAGgCCTCACCGGCCCCGAGGCGCATCCGCTGTCGCGTCCGCCGCAAGTC
GAGGGCGAGgCCGCCAGCCGCGCCATCCGCATCGCCGAGACGCTGGGTACGCCGCTGTACCTGGTGCATATT
TCCAGCCGCGAGGCGCTGGACGAGATCGCCTATGCCCGCGCCAAGGGCCAGCCGGTCTACGGCGAGGTGCTG
GCCGGGCACCTGCTGCTCGACGACAGCGTCTACCGCCACCCGGACTGGGCCACCGCGGCCGGCTACGTGATG
AGCCCGCCGTTCCGTCCCGTCGAACACCAGGAAGCGCTGTGGCGCGGCCTGCAGTCCGGCAACCTGCATACC
ACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAGGCCATGGGCCGCGACGACTTCAGCAAGATTCCC
AATGGCACGGCCGGCATTGAGGACCGCATGGCGTTACTGTGGGACGCCGGGGTCAACAGCGGGCGCCTGTCG
ATGCACGAGTTCGTCGCGCTGACCTCCACCAACACCGCGAAGATCTTCAACCTGTTCCCGCGCAAGGGCGCG
ATCCGCGTCGGCGCGGATGCCGACCTGGTGCTCTGGGACCCGCAGGGCAGCCGCACCCTCTCGGCCGCCACC
CACCACCAGCGGGTCGATTTCAACATCTTCGAAGGTCGCACCGTGCGCGGCATTCCCAGCCACACCATCAGC
CAGGGCAAGCTGCTCTGGGCCGCCGGCGACCTGCGCGCCGAACCCGGCGCCGGACGCTACGTGGAGCGCCCG
GCCTACCCGTCGGTGTACGAAGTGCTCGGCCGCCGCGCCGAACGCCAGCGCCCGGTCGCCGTCGAGCGCTGA
Translation:
MSLLIRGATVVTHEESYRADVLCANGLIQAIGENLETPSGCDVLDGGGQYLMPGGIDPHTHMQLPFMGTVAS
EDFFSGTAAGLAGGTTSIIDFVIPNPRQSLLEAFHTWRGWAQKSAADYGFHVAITWWSDEVAREMGELVAQH
GVNSFKHFMAYKNAIMAADDTLVASFERCLELGAVPTVHAENGELVFHLQQKLLAQGLTGPEAHPLSRPPQV
EGEAASRAIRIAETLGTPLYLVHISSREALDEIAYARAKGQPVYGEVLAGHLLLDDSVYRHPDWATAAGYVM
SPPFRPVEHQEALWRGLQSGNLHTTATDHCCFCAEQKAMGRDDFSKIPNGTAGIEDRMALLWDAGVNSGRLS
MHEFVALTSTNTAKIFNLFPRKGAIRVGADADLVLWDPQGSRTLSAATHHQRVDFNIFEGRTVRGIPSHTIS
QGKLLWAAGDLRAEPGAGRYVERPAYPSVYEVLGRRAERQRPVAVER*
AnnotationID:828GeneID:819
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 12:12:03
Gene Name:dhT
Confidence Code:2
GeneProduct:dihydropyrimidinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:D-hydantoinase
Functional Category Confidence Code:5
COGs:cd01314,COG0044
Secondary Functional Category(ies):
EC Number:3.5.2.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC00209.1| (U84197) D-hydantoinase [Pseudomonas putida]
          Length = 495

 Score =  850 bits (2197), Expect = 0.0
 Identities = 410/450 (91%), Positives = 431/450 (95%)

>dbj|BAC52880.1| (AP005962) dihydropyrimidinase [Bradyrhizobium japonicum USDA 110]
          Length = 484

 Score =  568 bits (1464), Expect = e-161
 Identities = 269/479 (56%), Positives = 352/479 (73%)






 
Structural Features:
cd01314, D-HYD, D-hydantoinases (D-HYD) also called dihydropyrimidases (DHPase) 
and related proteins
CD-Length = 447 residues, 100.0% aligned
Score =  685 bits (1769), Expect = 0.0

COG0044, PyrC, Dihydroorotase and related cyclic amidohydrolases 
[Nucleotide transport and metabolism]
CD-Length = 430 residues, 100.0% aligned
Score =  387 bits (996), Expect = 1e-108
Identities = 160/449 (35%), Positives = 229/449 (51%), Gaps = 20/449 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1641




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18783




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33763




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:819_cdd.html


    		




 
URL:

















    ReferenceID:35346




  
Author/Investigator(s):
        LaPointe G, Viau S, LeBlanc D, Robert N, Morin A.





  
Title:
        Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms.




  
PubMed:
        8161181


MedLine:
        




    
Source:
Appl Environ Microbiol. 1994 Mar;60(3):888-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8161181

















    ReferenceID:35347




  
Author/Investigator(s):
        Mukohara Y, Ishikawa T, Watabe K, Nakamura H.





  
Title:
        A thermostable hydantoinase of Bacillus stearothermophilus NS1122A: cloning, sequencing, and high expression of the enzyme gene, and some properties of the expressed enzyme.




  
PubMed:
        7765480


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1994 Sep;58(9):1621-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7765480

















    ReferenceID:35348




  
Author/Investigator(s):
        Chien HR, Jih YL, Yang WY, Hsu WH.





  
Title:
        Identification of the open reading frame for the Pseudomonas putida D-hydantoinase gene and expression of the gene in Escherichia coli.




  
PubMed:
        9434154


MedLine:
        




    
Source:
Biochim Biophys Acta. 1998 Jan 7;1395(1):68-77.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9434154

















    ReferenceID:35349




  
Author/Investigator(s):
        Chen HY, Tsai H.





  
Title:
        Cloning, sequencing, and expression in Escherichia coli of D-hydantoinase gene from Pseudomonas putida.




  
PubMed:
        9928097


MedLine:
        




    
Source:
Ann N Y Acad Sci. 1998 Dec 13;864:234-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9928097

















    ReferenceID:35350




  
Author/Investigator(s):
        Hils M, Munch P, Altenbuchner J, Syldatk C, Mattes R.





  
Title:
        Cloning and characterization of genes from Agrobacterium sp. IP I-671 involved in hydantoin degradation.




  
PubMed:
        11778878


MedLine:
        




    
Source:
Appl Microbiol Biotechnol. 2001 Dec;57(5-6):680-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11778878









           
Homologs By Global Alignment 

           

       



     
Gene ID:819
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1471
                           PA0441_tr
                           translation of PA0441
                           479
                           99.99
                           99.99
                           0
                           2526.0
     

     
     
53542
                           gb|AAG03830.1|AE004481_6
                           (AE004481) dihydropyrimidinase [Pseudomonas aeruginosa PAO1]
                           479
                           99.99
                           99.99
                           0
                           2526.0
     

     
     
53543
                           gb|AAQ90019.1|
                           (AY387829) D-hydantoinase [Pseudomonas putida]
                           479
                           92.06
                           97.07
                           0
                           2362.0
     

     
     
53544
                           gb|AAC00209.1|
                           (U84197) D-hydantoinase [Pseudomonas putida]
                           497
                           84.90
                           89.73
                           20
                           2199.0
     

     
     
53545
                           dbj|BAC52880.1|
                           (AP005962) dihydropyrimidinase [Bradyrhizobium japonicum USDA 110]
                           484
                           55.57
                           72.72
                           5
                           1464.0
     

     






      

          
GC ORFID: 43821How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0441Glimmer Score: 

          
Start: 6312348Stop: 6310909

          
Length: 1440


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0441 translation of PA0441Homolog Bit Score 977.0

          
Other Homologs:  t_PA0441 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACTCACGAAGAGAGTTATCGAGCCGACGTGCTCTGTGCC 
AACGGCCTCATCCAGGCCATCGGCGAGAACCTCGAAACCCCCTCCGGCTGCGACGTCCTCGACGGCGGTGGC 
CAGTACCTGATGCCGGGCGGCATCGACCCCCATACCCACATGCAGTTGCCCTTCATGGGCACGGTGGCCAGC 
GAGGACTTCTTCAGCGGCACCGCGGCCGGGCTGGCCGGAGGGACCACCTCGATCATCGACTTCGTCATCCCC 
AACCCGCGACAGTCGCTGCTGGAGGCCTTCCACACCTGGCGCGGCTGGGCGCAGAAATCCGCTGCGGACTAC 
GGCTTCCACGTCGCCATCACCTGGTGGAGCGACGAGGTCGCCCGGGAAATGGGCGAGCTGGTGGCACAGCAC 
GGGGTGAACAGCTTCAAGCACTTCATGGCCTACAAGAACGCCATCATGGCCGCCGACGATACCCTGGTGGCC 
AGCTTCGAGCGCTGCCTGGAGCTGGGCGCGGTGCCGACGGTGCACGCGGAGAACGGCGAACTGGTCTTCCAC 
CTGCAGCAGAAACTCCTCGCCCAAGgCCTCACCGGCCCCGAGGCGCATCCGCTGTCGCGTCCGCCGCAAGTC 
GAGGGCGAGgCCGCCAGCCGCGCCATCCGCATCGCCGAGACGCTGGGTACGCCGCTGTACCTGGTGCATATT 
TCCAGCCGCGAGGCGCTGGACGAGATCGCCTATGCCCGCGCCAAGGGCCAGCCGGTCTACGGCGAGGTGCTG 
GCCGGGCACCTGCTGCTCGACGACAGCGTCTACCGCCACCCGGACTGGGCCACCGCGGCCGGCTACGTGATG 
AGCCCGCCGTTCCGTCCCGTCGAACACCAGGAAGCGCTGTGGCGCGGCCTGCAGTCCGGCAACCTGCATACC 
ACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAGGCCATGGGCCGCGACGACTTCAGCAAGATTCCC 
AATGGCACGGCCGGCATTGAGGACCGCATGGCGTTACTGTGGGACGCCGGGGTCAACAGCGGGCGCCTGTCG 
ATGCACGAGTTCGTCGCGCTGACCTCCACCAACACCGCGAAGATCTTCAACCTGTTCCCGCGCAAGGGCGCG 
ATCCGCGTCGGCGCGGATGCCGACCTGGTGCTCTGGGACCCGCAGGGCAGCCGCACCCTCTCGGCCGCCACC 
CACCACCAGCGGGTCGATTTCAACATCTTCGAAGGTCGCACCGTGCGCGGCATTCCCAGCCACACCATCAGC 
CAGGGCAAGCTGCTCTGGGCCGCCGGCGACCTGCGCGCCGAACCCGGCGCCGGACGCTACGTGGAGCGCCCG 
GCCTACCCGTCGGTGTACGAAGTGCTCGGCCGCCGCGCCGAACGCCAGCGCCCGGTCGCCGTCGAGCGCTGA