ViewGene
Locus Name:
GeneID:
Location:

GeneID:818
TrivialName: PA14_71890
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71890
PAO1 Ortholog Locus:PA5445
Sequence Length:1494
Protein Length:497
Start: 6406758
Stop: 6408251
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08830.1|AE004957_5 (AE004957) probable coenzyme A transferase [Pseudomonas aeruginosa PAO1]
Identities = 497/497 (100%), Positives = 497/497 (100%)
Sequence:
ATGTACCGTGATCGCGTGCGCCTGCCCTCCCTGCTCGACAAGGTGATGAGTGCGGCCGAAGCCGCCGACCTG
ATCCAGGACGGCATGACCGTCGGCATGAGCGGTTTCACCCGCGCCGGCGAGGCCAAGGCCGTACCCCAGGCG
CTGGCCATGCGCGCCAAGGAACGTCCGCTGCGTATCAGCCTGATGACCGGCGCCAGCCTCGGCAACGACCTC
GACAAGCAGCTCACCGAGGCCGGCGTGCTCGCCCGGCGCATGCCGTTCCAGGTCGACAGCACCCTGCGCAAG
GCGATCAACGCCGGCGAAGTGATGTTCATCGACCAGCACCTCTCGGAAACCGTCGAGCAGTTGCGCAACCAC
CAGCTGAAGCTGCCGGACATCGCCGTCATCGAAGCCGCCGCGATCACCGAGCAGGGCCACATCGTGCCAACC
ACCTCGGTGGGCAACTCGGCGAGCTTCGCGATCTTCGCCAAGCAGGTCATCGTCGAGATCAACCTGGCCCAC
AGCACCAACCTGGAAGGCCTGCACGACATCTATATCCCGACCTACCGGCCGACCCGCACGCCGATCCCGCTG
ACCCGCGTCGACGACCGCATCGGCAGCACGGCGATCCCGATCCCGCCGGAGAAGATCGTCGCCATCGTCATC
AACGACCAGCCGGACTCGCCATCCACCGTGCTGCCGCCGGACGGCGAGACCCAGGCCATCGCCAACCACCTG
ATCGATTTCTTCAAGCGCGAGGTCGACGCCGGACGCATGAGCAACAGCCTCGGCCCGCTGCAGGCCGGTATC
GGCAGCATCGCCAACGCGGTGATGTGCGGCCTGATCGAGTCACCGTTCGAGAACCTGACCATGTACTCCGAA
GTGCTGCAGGACTCGACCTTCGACCTGATCGACGCCGGCAAGCTGCGCTTCGCCTCGGGCAGCTCGATCACC
CTGTCGCCGCGGCGCAACGCCGACGTGTTCGGTAACCTGGAGCGTTACAAGGACAAGCTGGTGCTGCGCCCG
CAGGAAATCTCCAACCACCCCGAGGTGGTCCGTCGCCTGGGCATCATCGGCATCAATACGGCGCTGGAGTTC
GACATCTACGGCAACGTCAACTCGACCCACGTCGGCGGCACCAAGATGATGAACGGCATCGGCGGCTCCGGC
GACTTCGCCCGCAACGCGCACCTGGCGATCTTCGTCACCAAGTCGATCGCCAAGGGCGGCAACATTTCCAGC
GTGGTGCCGATGGTCAGCCACGTCGACCACACCGAGCACGACGTCGACATCCTCGTCACCGAGCAGGGCCTC
GCCGACCTGCGCGGCCTGGCGCCTCGCGAACGGGCCCGGGTGATCATCGAGAACTGTGTCCACCCGTCCTAC
CAGGCACCGCTGCTGGACTACTTCGAGGCGGCTTGCGCGAAAGGCGGCCACACGCCGCATCTGTTGCGCGAG
GCGCTGGCCTGGCACCTGAACCTGGAAGAACGTGGACACATGCTGGCCGGCTGA
Translation:
MYRDRVRLPSLLDKVMSAAEAADLIQDGMTVGMSGFTRAGEAKAVPQALAMRAKERPLRISLMTGASLGNDL
DKQLTEAGVLARRMPFQVDSTLRKAINAGEVMFIDQHLSETVEQLRNHQLKLPDIAVIEAAAITEQGHIVPT
TSVGNSASFAIFAKQVIVEINLAHSTNLEGLHDIYIPTYRPTRTPIPLTRVDDRIGSTAIPIPPEKIVAIVI
NDQPDSPSTVLPPDGETQAIANHLIDFFKREVDAGRMSNSLGPLQAGIGSIANAVMCGLIESPFENLTMYSE
VLQDSTFDLIDAGKLRFASGSSITLSPRRNADVFGNLERYKDKLVLRPQEISNHPEVVRRLGIIGINTALEF
DIYGNVNSTHVGGTKMMNGIGGSGDFARNAHLAIFVTKSIAKGGNISSVVPMVSHVDHTEHDVDILVTEQGL
ADLRGLAPRERARVIIENCVHPSYQAPLLDYFEAACAKGGHTPHLLREALAWHLNLEERGHMLAG*
AnnotationID:827GeneID:818
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 20:08:49
Gene Name:
Confidence Code:3
GeneProduct:putative coenzyme A transferase
Cell Localization:()
Synonyms:psecoA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative acetyl-CoA hydrolase/transferase
Functional Category Confidence Code:5
COGs:COG0427,pfam02550
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65788.1|AE016205_1 (AE016774) acetyl-CoA hydrolase/transferase family protein
           [Pseudomonas putida KT2440]
          Length = 497

 Score =  872 bits (2253), Expect = 0.0
 Identities = 442/496 (89%), Positives = 465/496 (93%)

>gb|AAO58884.1| (AE016875) acetyl-CoA hydrolase/transferase family protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 497

 Score =  776 bits (2004), Expect = 0.0
 Identities = 391/495 (78%), Positives = 437/495 (88%)

>gb|AAL41417.1| (AE009010) coenzyme A transferase [Agrobacterium tumefaciens str.
           C58 (U. Washington)]
          Length = 498

 Score =  709 bits (1829), Expect = 0.0
 Identities = 363/495 (73%), Positives = 408/495 (82%)








 
Structural Features:
COG0427, ACH1, Acetyl-CoA hydrolase [Energy production and conversion]
CD-Length = 501 residues, 100.0% aligned
Score =  629 bits (1624), Expect = 0.0
Identities = 282/506 (55%), Positives = 345/506 (68%), Gaps = 15/506 (2%)

pfam02550, AcetylCoA_hydro, Acetyl-CoA hydrolase/transferase
CD-Length = 473 residues,  98.5% aligned
Score =  343 bits (881), Expect = 3e-95
Identities = 185/501 (36%), Positives = 251/501 (50%), Gaps = 55/501 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1639




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18781




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34666




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:818_cdd.html


    		




 
URL:

















    ReferenceID:35628




  
Author/Investigator(s):
        Rocchetta HL, Burrows LL, Pacan JC, Lam JS.





  
Title:
        Three rhamnosyltransferases responsible for assembly of the A-band D-rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for the initiation of both A-band and B-band lipopolysaccharide synthesis.




  
PubMed:
        9680202


MedLine:
        




    
Source:
Mol Microbiol. 1998 Jun;28(6):1103-19.

Erratum in:
    * Mol Microbiol 1998 Dec;30(5):1131.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9680202









           
Homologs By Global Alignment 

           

       



     
Gene ID:818
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1470
                           PA5445_tr
                           translation of PA5445
                           497
                           99.99
                           99.99
                           0
                           2525.0
     

     
     
53506
                           gb|AAG08830.1|AE004957_5
                           (AE004957) probable coenzyme A transferase [Pseudomonas aeruginosa PAO1]
                           497
                           99.99
                           99.99
                           0
                           2525.0
     

     
     
53507
                           gb|AAC38773.1|
                           (AF010184) coenzyme A transferase PsecoA [Pseudomonas aeruginosa]
                           497
                           99.59
                           99.59
                           0
                           2516.0
     

     
     
53508
                           gb|AAN65788.1|AE016205_1
                           (AE016774) acetyl-CoA hydrolase/transferase family protein [Pseudomonas putida KT2440]
                           497
                           88.93
                           93.56
                           0
                           2253.0
     

     
     
53509
                           gb|AAO58884.1|
                           (AE016875) acetyl-CoA hydrolase/transferase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           497
                           78.67
                           87.92
                           0
                           2001.0
     

     






      

          
GC ORFID: 43820How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5445Glimmer Score: 

          
Start: 5670447Stop: 5671940

          
Length: 1494


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5445 translation of PA5445Homolog Bit Score 977.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2886 (ORF: BLASTX 771.0), t_PA5445 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTACCGTGATCGCGTGCGCCTGCCCTCCCTGCTCGACAAGGTGATGAGTGCGGCCGAAGCCGCCGACCTG 
ATCCAGGACGGCATGACCGTCGGCATGAGCGGTTTCACCCGCGCCGGCGAGGCCAAGGCCGTACCCCAGGCG 
CTGGCCATGCGCGCCAAGGAACGTCCGCTGCGTATCAGCCTGATGACCGGCGCCAGCCTCGGCAACGACCTC 
GACAAGCAGCTCACCGAGGCCGGCGTGCTCGCCCGGCGCATGCCGTTCCAGGTCGACAGCACCCTGCGCAAG 
GCGATCAACGCCGGCGAAGTGATGTTCATCGACCAGCACCTCTCGGAAACCGTCGAGCAGTTGCGCAACCAC 
CAGCTGAAGCTGCCGGACATCGCCGTCATCGAAGCCGCCGCGATCACCGAGCAGGGCCACATCGTGCCAACC 
ACCTCGGTGGGCAACTCGGCGAGCTTCGCGATCTTCGCCAAGCAGGTCATCGTCGAGATCAACCTGGCCCAC 
AGCACCAACCTGGAAGGCCTGCACGACATCTATATCCCGACCTACCGGCCGACCCGCACGCCGATCCCGCTG 
ACCCGCGTCGACGACCGCATCGGCAGCACGGCGATCCCGATCCCGCCGGAGAAGATCGTCGCCATCGTCATC 
AACGACCAGCCGGACTCGCCATCCACCGTGCTGCCGCCGGACGGCGAGACCCAGGCCATCGCCAACCACCTG 
ATCGATTTCTTCAAGCGCGAGGTCGACGCCGGACGCATGAGCAACAGCCTCGGCCCGCTGCAGGCCGGTATC 
GGCAGCATCGCCAACGCGGTGATGTGCGGCCTGATCGAGTCACCGTTCGAGAACCTGACCATGTACTCCGAA 
GTGCTGCAGGACTCGACCTTCGACCTGATCGACGCCGGCAAGCTGCGCTTCGCCTCGGGCAGCTCGATCACC 
CTGTCGCCGCGGCGCAACGCCGACGTGTTCGGTAACCTGGAGCGTTACAAGGACAAGCTGGTGCTGCGCCCG 
CAGGAAATCTCCAACCACCCCGAGGTGGTCCGTCGCCTGGGCATCATCGGCATCAATACGGCGCTGGAGTTC 
GACATCTACGGCAACGTCAACTCGACCCACGTCGGCGGCACCAAGATGATGAACGGCATCGGCGGCTCCGGC 
GACTTCGCCCGCAACGCGCACCTGGCGATCTTCGTCACCAAGTCGATCGCCAAGGGCGGCAACATTTCCAGC 
GTGGTGCCGATGGTCAGCCACGTCGACCACACCGAGCACGACGTCGACATCCTCGTCACCGAGCAGGGCCTC 
GCCGACCTGCGCGGCCTGGCGCCTCGCGAACGGGCCCGGGTGATCATCGAGAACTGTGTCCACCCGTCCTAC 
CAGGCACCGCTGCTGGACTACTTCGAGGCGGCTTGCGCGAAAGGCGGCCACACGCCGCATCTGTTGCGCGAG 
GCGCTGGCCTGGCACCTGAACCTGGAAGAACGTGGACACATGCTGGCCGGCTGA