ViewGene
Locus Name:
GeneID:
Location:

GeneID:812
TrivialName: PA14_23990
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23990
PAO1 Ortholog Locus:PA3103
Sequence Length:1509
Protein Length:502
Start: 2084868
Stop: 2086376
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06491.1|AE004734_9 (AE004734) general secretion pathway protein E [Pseudomonas aeruginosa PAO1]
Identities = 502/502 (100%), Positives = 502/502 (100%)
Sequence:
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCC
AAGCGCCAGGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTG
GCGGCGATCGCCGAGGCGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTC
AACCAGGCCCTGGCGGCCTCCTACCAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGC
AGCCTGGACCTCGCCGCGCTGGCCGACCAGGTCCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCG
CCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGATCCGCGAGAACGCCTCGGACATCCACCTGGAA
ACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTGCGCGAGGTGCTGGAGCCCAAGCGC
GAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGCCGAGAAGCGTATTCCC
CAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGCCCTCGGCC
AACGGCGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCC
ACCGGCTCGGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTC
ACCGTCGAGGATCCGATCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATG
ACCTTCGCCCGCGGCCTGCGGGCGATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGAC
CGGGAGACCGCCGAGATCGCGGTGCAGGCTTCGCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAAC
AGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCATCGAGCCGTTCCTGCTGTCCTCCTCCATGCTT
GGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAGGAGCCCTACCGCGCCGACGAGGCC
GAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCGCGGCTGCGGCGAGTGC
CACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGCGCTCGCTG
ATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCAAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACTACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA
Translation:
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAF
NQALAASYQHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLE
TFEKRLVVRFRVDGVLREVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSA
NGERVVLRLLDKQAGRLNLQHLGMSERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNIL
TVEDPIEYHLEGIGQTQVNAKVDMTFARGLRAILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTN
SAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACKEPYRADEAECALLGVDPAAPPTLHRARGCGEC
HQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDDGRRKVLEGVTTVEEVLRVTREE*
AnnotationID:821GeneID:812
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 06:06:24
Gene Name:xcpR
Confidence Code:1
GeneProduct: general secretion pathway protein E
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:ATPase PulE/Tfp pilus assembly pathway protein
Functional Category Confidence Code:5
COGs:COG2804,pfam00437
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC83356.1| (AF092918) outer membrane secretion protein R [Pseudomonas
           alcaligenes]
          Length = 503

 Score =  745 bits (1924), Expect = 0.0
 Identities = 382/495 (77%), Positives = 431/495 (87%), Gaps = 1/495 (0%)

>gb|AAN53254.1|AE015466_14 (AE015466) general secretion pathway protein E [Shewanella
           oneidensis MR-1]
          Length = 521

 Score =  610 bits (1572), Expect = e-174
 Identities = 315/489 (64%), Positives = 387/489 (79%), Gaps = 7/489 (1%)

>gb|AAO09379.1|AE016799_277 (AE016799) Type II secretory pathway, ATPase EpsE [Vibrio
           vulnificus CMCP6]
          Length = 499

 Score =  594 bits (1532), Expect = e-169
 Identities = 314/501 (62%), Positives = 384/501 (76%), Gaps = 12/501 (2%)






 
Structural Features:
COG2804, PulE, Type II secretory pathway, ATPase PulE/Tfp 
pilus assembly pathway, ATPase PilB 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 500 residues,  97.6% aligned
Score =  582 bits (1501), Expect = 3e-167
Identities = 274/493 (55%), Positives = 348/493 (70%), Gaps = 8/493 (1%)

pfam00437, GSPII_E, Type II/IV secretion system protein.
CD-Length = 283 residues, 100.0% aligned
Score =  287 bits (737), Expect = 2e-78
Identities = 143/291 (49%), Positives = 180/291 (61%), Gaps = 20/291 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1627




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18769




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32421




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:812_cdd.html


    		




 
URL:

















    ReferenceID:33467




  
Author/Investigator(s):
        Ball G, Durand E, Lazdunski A, Filloux A.





  
Title:
        A novel type II secretion system in Pseudomonas aeruginosa.




  
PubMed:
        11985723


MedLine:
        




    
Source:
Mol Microbiol. 2002 Jan;43(2):475-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11985723

















    ReferenceID:33468




  
Author/Investigator(s):
        Ball G, Chapon-Herve V, Bleves S, Michel G, Bally M.





  
Title:
        Assembly of XcpR in the cytoplasmic membrane is required for extracellular protein secretion in Pseudomonas aeruginosa.




  
PubMed:
        9882649


MedLine:
        




    
Source:
J Bacteriol. 1999 Jan;181(2):382-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9882649

















    ReferenceID:33469




  
Author/Investigator(s):
        Kagami Y, Ratliff M, Surber M, Martinez A, Nunn DN.





  
Title:
        Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin-like component XcpT by the cytoplasmic component XcpR.




  
PubMed:
        9466269


MedLine:
        




    
Source:
Mol Microbiol. 1998 Jan;27(1):221-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9466269

















    ReferenceID:33470




  
Author/Investigator(s):
        Turner LR, Olson JW, Lory S.





  
Title:
        The XcpR protein of Pseudomonas aeruginosa dimerizes via its N-terminus.




  
PubMed:
        9426126


MedLine:
        




    
Source:
Mol Microbiol. 1997 Dec;26(5):877-87.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9426126

















    ReferenceID:33471




  
Author/Investigator(s):
        Parche S, Geissdorfer W, Hillen W.





  
Title:
        Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1.




  
PubMed:
        9226277


MedLine:
        




    
Source:
J Bacteriol. 1997 Jul;179(14):4631-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9226277









           
Homologs By Global Alignment 

           

       



     
Gene ID:812
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1458
                           PA3103_tr
                           translation of PA3103
                           502
                           99.99
                           99.99
                           0
                           2528.0
     

     
     
53159
                           emb|CAA44533.1|
                           (X62666) secretion protein XcpR [Pseudomonas aeruginosa]
                           502
                           99.99
                           99.99
                           0
                           2528.0
     

     
     
53158
                           gb|AAG06491.1|AE004734_9
                           (AE004734) general secretion pathway protein E [Pseudomonas aeruginosa PAO1]
                           502
                           99.99
                           99.99
                           0
                           2528.0
     

     
     
53160
                           gb|AAC83356.1|
                           (AF092918) outer membrane secretion protein R [Pseudomonas alcaligenes]
                           506
                           76.28
                           85.96
                           7
                           1927.0
     

     
     
53161
                           emb|CAA47126.1|
                           (X66504) ExeE [Aeromonas hydrophila]
                           502
                           63.54
                           77.29
                           1
                           1576.0
     

     






      

          
GC ORFID: 43814How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3103Glimmer Score: 

          
Start: 2869063Stop: 2867555

          
Length: 1509


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3103 translation of PA3103Homolog Bit Score 978.0

          
Other Homologs:  t_PA0686 (ORF: BLASTX 496.0), t_PA3103 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCC 
AAGCGCCAGGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTG 
GCGGCGATCGCCGAGGCGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTC 
AACCAGGCCCTGGCGGCCTCCTACCAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGC 
AGCCTGGACCTCGCCGCGCTGGCCGACCAGGTCCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCG 
CCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGATCCGCGAGAACGCCTCGGACATCCACCTGGAA 
ACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTGCGCGAGGTGCTGGAGCCCAAGCGC 
GAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGCCGAGAAGCGTATTCCC 
CAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGCCCTCGGCC 
AACGGCGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACCTGGGCATG 
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCC 
ACCGGCTCGGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTC 
ACCGTCGAGGATCCGATCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATG 
ACCTTCGCCCGCGGCCTGCGGGCGATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGAC 
CGGGAGACCGCCGAGATCGCGGTGCAGGCTTCGCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAAC 
AGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCATCGAGCCGTTCCTGCTGTCCTCCTCCATGCTT 
GGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAGGAGCCCTACCGCGCCGACGAGGCC 
GAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCGCGGCTGCGGCGAGTGC 
CACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGCGCTCGCTG 
ATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCAAGCATCCGCGACGAC 
GGTCGGCGCAAGGTGCTGGAAGGCGTCACTACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA